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The map label for this gene is rpoS [H]

Identifier: 148358826

GI number: 148358826

Start: 1492775

End: 1493800

Strand: Direct

Name: rpoS [H]

Synonym: LPC_0709

Alternate gene names: 148358826

Gene position: 1492775-1493800 (Clockwise)

Preceding gene: 148358825

Following gene: 148358828

Centisome position: 41.74

GC content: 39.18

Gene sequence:

>1026_bases
ATGCAAGATGAAGAAGAGCCAATTAAGGACAAGGAATTTAAAGAAGAGGAATGGTCTGAGCCAGATGATGACTCTCTGTT
GTCAGAAGAAGATGTTGAACCTGATATCGAAAAAATGGAAGAAGATCTTGATGAATTTCCAGAGTTTACTGATGATGAAG
TTTTTCCTTCATTTCAGCGCGGGAAAAACATTGCTAAGGCTTTAGATGCGACTCAACTTTACTTGGGTGAAATTGGCTTT
TCTCCTTTGTTAACGGCTGAAGAAGAAATCCATTATGCGACTCTTGCTTTAAAAGGTGATATGGAAGCACGCAAGAAAAT
GATTGAGTCGAATTTGCGCCTGGTGGTTAAAATAGCCAGACGTTATCTTAATAGAGGATTACCTCTTCTGGATCTAATCG
AAGAGGGTAACCTGGGTTTAATGAAGTCTGTTGAGAAATTTGACCCCAAGCGAGGATTCCGTTTTTCAACTTATGCGACC
TGGTGGATTAGGCAAACTATTGAGCGCGCAATTATGAATCAAACGAGAACTATCCGCTTGCCTATTCATGTCGTGAAAGA
ACTTAATGTTTATCTCAGAGCAGCAAGACAATTGACGCAAAAACTGGATCATGAACCATCACCTGAAGAAATAGCAGAAA
TGGTGGATAAGCCTTTGGAGGATGTCCAAAAATTATTGGGATTGAATGATAAAGTGGCTTCAGTTGATACTCCAATTGGA
TTTGATGAAACCAAATCTTTATTAGATACAATTGCTGATGAAAACAGTTTAAATCCTGCAGAGCTGCTAACCAATGAGAA
TTTAAGGCAGCATATAGAATCTCTTCTGGATAAATTAACTGAGAATCAACAGCAGGTTATCGCAAGACGTTTTGGGTTAA
GAGGTTTTGAAAAAGCGACTCTGGAAGACGTTGGTAAGGAAATTGATTTGACCCGAGAACGCGTTCGCCAAATTCAAGTT
GAAGCTTTGAAGACACTTAGAACTTTGTTAGAGCGGGTTGGACTAACACAAGAGGATTTGTTTTGA

Upstream 100 bases:

>100_bases
CTACAAAAAGGTTGACATATAACTCATAGTTATAGCAACAATAGGCTTGTTAAGAAGTAAAAAACTATTTCAAGGCATAG
CTAAACATATAGGTGATACA

Downstream 100 bases:

>100_bases
TTTAACCTAGTTAACTATATGCAAGTTGGGTCGAAATTGACCCAACTGAAGCCAAATTAATTAATTTCCGTCAATTTTAC
ATCTTAAAAAGCAGTCAATT

Product: stationary phase specific sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MQDEEEPIKDKEFKEEEWSEPDDDSLLSEEDVEPDIEKMEEDLDEFPEFTDDEVFPSFQRGKNIAKALDATQLYLGEIGF
SPLLTAEEEIHYATLALKGDMEARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYAT
WWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKPLEDVQKLLGLNDKVASVDTPIG
FDETKSLLDTIADENSLNPAELLTNENLRQHIESLLDKLTENQQQVIARRFGLRGFEKATLEDVGKEIDLTRERVRQIQV
EALKTLRTLLERVGLTQEDLF

Sequences:

>Translated_341_residues
MQDEEEPIKDKEFKEEEWSEPDDDSLLSEEDVEPDIEKMEEDLDEFPEFTDDEVFPSFQRGKNIAKALDATQLYLGEIGF
SPLLTAEEEIHYATLALKGDMEARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYAT
WWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKPLEDVQKLLGLNDKVASVDTPIG
FDETKSLLDTIADENSLNPAELLTNENLRQHIESLLDKLTENQQQVIARRFGLRGFEKATLEDVGKEIDLTRERVRQIQV
EALKTLRTLLERVGLTQEDLF
>Mature_341_residues
MQDEEEPIKDKEFKEEEWSEPDDDSLLSEEDVEPDIEKMEEDLDEFPEFTDDEVFPSFQRGKNIAKALDATQLYLGEIGF
SPLLTAEEEIHYATLALKGDMEARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYAT
WWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKPLEDVQKLLGLNDKVASVDTPIG
FDETKSLLDTIADENSLNPAELLTNENLRQHIESLLDKLTENQQQVIARRFGLRGFEKATLEDVGKEIDLTRERVRQIQV
EALKTLRTLLERVGLTQEDLF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=276, Percent_Identity=68.4782608695652, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1789448, Length=225, Percent_Identity=48.4444444444444, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1789871, Length=279, Percent_Identity=29.7491039426523, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788231, Length=207, Percent_Identity=27.0531400966184, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 39468; Mature: 39468

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDEEEPIKDKEFKEEEWSEPDDDSLLSEEDVEPDIEKMEEDLDEFPEFTDDEVFPSFQR
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHH
GKNIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDMEARKKMIESNLRLVVKIAR
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHH
RYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYATWWIRQTIERAIMNQTRTIRL
HHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEH
PIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKPLEDVQKLLGLNDKVASVDTPIG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCC
FDETKSLLDTIADENSLNPAELLTNENLRQHIESLLDKLTENQQQVIARRFGLRGFEKAT
HHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LEDVGKEIDLTRERVRQIQVEALKTLRTLLERVGLTQEDLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MQDEEEPIKDKEFKEEEWSEPDDDSLLSEEDVEPDIEKMEEDLDEFPEFTDDEVFPSFQR
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHH
GKNIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDMEARKKMIESNLRLVVKIAR
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHH
RYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYATWWIRQTIERAIMNQTRTIRL
HHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEH
PIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKPLEDVQKLLGLNDKVASVDTPIG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCC
FDETKSLLDTIADENSLNPAELLTNENLRQHIESLLDKLTENQQQVIARRFGLRGFEKAT
HHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LEDVGKEIDLTRERVRQIQVEALKTLRTLLERVGLTQEDLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]