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The map label for this gene is hisH [H]

Identifier: 148358782

GI number: 148358782

Start: 1446449

End: 1447048

Strand: Reverse

Name: hisH [H]

Synonym: LPC_0663

Alternate gene names: 148358782

Gene position: 1447048-1446449 (Counterclockwise)

Preceding gene: 148358783

Following gene: 148358781

Centisome position: 40.46

GC content: 37.33

Gene sequence:

>600_bases
ATGATTGCTGTGATTGATGTCAGTGGTAATAATTTAACTTCTCTGACAAATGCATTGATTCGATTGGGAGGGCATTTTGC
ACTGACCCATGATGCTGAAGAAATACAAAAAGCAAGCCATGTCATTTTGCCAGGGGTTGGAACAGCACGTTCTGGTATGA
AAGCTTTGCAGCAAAATGGGCTAATAGACGTTTTAAGAACTTTAACCCAACCGCTTCTTGGCATCTGTCTTGGAATGCAA
TTATTGCTTGAATACAGCGAAGAAGACGACATACCCTGCCTGGGTTTGATTCCTGGAGTTGCAGAGTTATTAAAAGCTGA
AAGAAATCATCCTGTTCCTCATATGGGATGGAACAATCTTCATTGGCAAAAAACATCATCATTACAACAAGGATTAAATA
ATAGTGATTATGTTTATTTTGTTCATAGCTATGCTCTAAAAGCAGATGATTATGCATTGGCTCGATGTCAGTATCATGAA
GAGTTCACTGCAGTTGTAAAAAAAGGCAATTTTTATGGTATGCAATTTCATCCAGAAAAATCAGCTGATGTTGGCATGGT
ATTACTAAATAACTTTTTATCTTTGGAGTCTACATGTTAG

Upstream 100 bases:

>100_bases
ATCACCACATGATCGAAGCATGCTTCAAAGTTTTAGGTAGAGCCTTGCGACAGGCATGCTCAAGAACGAATAATTATCTA
CCTTCAACCAAGGGTGTATT

Downstream 100 bases:

>100_bases
TTATTCCAGCTATTGATTTGCAATCTGGACGATGTGTGCGCTTAAAACAAGGACGGTTTGATCAAGTAACGCAATTTAGC
GTTTTTCCCATAGAAAGGGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit 2; IGP synthase subunit hisH 2; ImGP synthase subunit hisH 2; IGPS subunit hisH 2 [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAEEIQKASHVILPGVGTARSGMKALQQNGLIDVLRTLTQPLLGICLGMQ
LLLEYSEEDDIPCLGLIPGVAELLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCQYHE
EFTAVVKKGNFYGMQFHPEKSADVGMVLLNNFLSLESTC

Sequences:

>Translated_199_residues
MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAEEIQKASHVILPGVGTARSGMKALQQNGLIDVLRTLTQPLLGICLGMQ
LLLEYSEEDDIPCLGLIPGVAELLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCQYHE
EFTAVVKKGNFYGMQFHPEKSADVGMVLLNNFLSLESTC
>Mature_199_residues
MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAEEIQKASHVILPGVGTARSGMKALQQNGLIDVLRTLTQPLLGICLGMQ
LLLEYSEEDDIPCLGLIPGVAELLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCQYHE
EFTAVVKKGNFYGMQFHPEKSADVGMVLLNNFLSLESTC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=42.0512820512821, Blast_Score=166, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=35.0467289719626, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 21965; Mature: 21965

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAEEIQKASHVILPGVGTARSGMKALQQNG
CEEEEEECCCCHHHHHHHHHHHCCCEEEECCHHHHHHHCCEEECCCCCHHHHHHHHHHCC
LIDVLRTLTQPLLGICLGMQLLLEYSEEDDIPCLGLIPGVAELLKAERNHPVPHMGWNNL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCC
HWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCQYHEEFTAVVKKGNFYGMQFHPEK
CCCCHHHHHCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
SADVGMVLLNNFLSLESTC
CCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAEEIQKASHVILPGVGTARSGMKALQQNG
CEEEEEECCCCHHHHHHHHHHHCCCEEEECCHHHHHHHCCEEECCCCCHHHHHHHHHHCC
LIDVLRTLTQPLLGICLGMQLLLEYSEEDDIPCLGLIPGVAELLKAERNHPVPHMGWNNL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCC
HWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCQYHEEFTAVVKKGNFYGMQFHPEK
CCCCHHHHHCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
SADVGMVLLNNFLSLESTC
CCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA