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The map label for this gene is rlmI [H]

Identifier: 148358776

GI number: 148358776

Start: 1440954

End: 1442126

Strand: Direct

Name: rlmI [H]

Synonym: LPC_0657

Alternate gene names: 148358776

Gene position: 1440954-1442126 (Clockwise)

Preceding gene: 148358772

Following gene: 148358777

Centisome position: 40.29

GC content: 39.13

Gene sequence:

>1173_bases
ATGAAAGCGAAAGTTATTTTAGTTAGTGGAAAGCAAAACACTGTATTAAGAGGTCATCCCTGGATTTTCCCCAAAGCCAT
AGCTCAATATTCTGGCAAGCTAATTACAGGTGAATTGGTTGAGATAATTGGCTCTGAAGGTGAGAGTTTAGGATTCGGAG
CCTATAATGAGCACTCTTTATATAGAGTAAGGGTTCTTGCTCTCTCCAATGAACAGATAAATAGTTTCGATTACAAGTCT
TTAATTACTCATAGAATCAAGCAAGCCAGTCTGGTGAGACGATGTCTTGATTTACCAAATGAACAGACTAATGCTTATCG
ATTGTTCAACAGTGAAGCGGATGGACTCTCTGGTTTAACGATTGATTGCTTTAATGACTATTGTGTTATAGCAAGTTCTG
CCTATTGGGTAGAATTGAATAAATCGATTATTGTTCATGTATTACAAGAGTTACTCCCGCATAATCATATCATTTGGCTT
CCTCAAAACAAGCCATTATCTCAAGATGGCTGGAAAGAAATTCATACAGAGAAAAGTCAGGGTAGCACTCAGGTTTTGGA
AGAAGGTGTTCTGTATCATGTTGAATTTGCGCAAACCCAAAAAACTGGATTGTTTCTTGACCAAAGAGAAAATCACAAAA
GGATAGCAAAACTATCCAGGGGAAAAAGAGTCCTGGATCTTTATACTTTTACGGGAGGGTTTGCTTTGCACGCAGCTAAA
GCGGGAGCTCTTCATGTTACGGCTGTAGATAGCTCGGTGCAGGCAATAGAGCAGGCTAAAAATAATGCTGTTTTAAATCA
CTTAAATACCATTGAATTTATTAAGGCAGATGCCAGAGAATATCTGAAAATGGCTGGCGAATATGATGTGGTCATTCTTG
ACCCGCCAAAACTGGTTCCTTCACAAAAACATTTACAGCAAGCTAAAAATTATTACCGATTTTTACATAGAGAAGCATTT
AAATATATGAAACCAGGATCTTTATTGATGACTTGCAACTGTTCTTCAGCGCTGTCATCCCAGGATTTTTGTTCCCTGGT
AAGCGCTCAGGCTGTTGCGATGGGAAAGCAGGCGCGTATTTTAGGCGTATACGGACCAGCAAGCTGCCATCCTACCTTGG
CAGCTTTTCCAGAGGGAAATTATTTAACAGCTATTTTATTGGCATTGGTATAA

Upstream 100 bases:

>100_bases
AGGTATTTTGTAAATTGCCGCATATTTGTCCTTCTTTCCATTGCAAGTATAATTCTTGATAGTGTAGATTAACTGACTTT
TAGGTTTTGGGAAATAGAGA

Downstream 100 bases:

>100_bases
AAGGAACCTTGATTTTTAAATCAAGGATGGTGTTAGGTTGATTTTTTTTGTATAACATTTGATTAGTTTGGCTTTAATAT
GTATAATTGGCGCAAATCGC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKAKVILVSGKQNTVLRGHPWIFPKAIAQYSGKLITGELVEIIGSEGESLGFGAYNEHSLYRVRVLALSNEQINSFDYKS
LITHRIKQASLVRRCLDLPNEQTNAYRLFNSEADGLSGLTIDCFNDYCVIASSAYWVELNKSIIVHVLQELLPHNHIIWL
PQNKPLSQDGWKEIHTEKSQGSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKLSRGKRVLDLYTFTGGFALHAAK
AGALHVTAVDSSVQAIEQAKNNAVLNHLNTIEFIKADAREYLKMAGEYDVVILDPPKLVPSQKHLQQAKNYYRFLHREAF
KYMKPGSLLMTCNCSSALSSQDFCSLVSAQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAILLALV

Sequences:

>Translated_390_residues
MKAKVILVSGKQNTVLRGHPWIFPKAIAQYSGKLITGELVEIIGSEGESLGFGAYNEHSLYRVRVLALSNEQINSFDYKS
LITHRIKQASLVRRCLDLPNEQTNAYRLFNSEADGLSGLTIDCFNDYCVIASSAYWVELNKSIIVHVLQELLPHNHIIWL
PQNKPLSQDGWKEIHTEKSQGSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKLSRGKRVLDLYTFTGGFALHAAK
AGALHVTAVDSSVQAIEQAKNNAVLNHLNTIEFIKADAREYLKMAGEYDVVILDPPKLVPSQKHLQQAKNYYRFLHREAF
KYMKPGSLLMTCNCSSALSSQDFCSLVSAQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAILLALV
>Mature_390_residues
MKAKVILVSGKQNTVLRGHPWIFPKAIAQYSGKLITGELVEIIGSEGESLGFGAYNEHSLYRVRVLALSNEQINSFDYKS
LITHRIKQASLVRRCLDLPNEQTNAYRLFNSEADGLSGLTIDCFNDYCVIASSAYWVELNKSIIVHVLQELLPHNHIIWL
PQNKPLSQDGWKEIHTEKSQGSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKLSRGKRVLDLYTFTGGFALHAAK
AGALHVTAVDSSVQAIEQAKNNAVLNHLNTIEFIKADAREYLKMAGEYDVVILDPPKLVPSQKHLQQAKNYYRFLHREAF
KYMKPGSLLMTCNCSSALSSQDFCSLVSAQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAILLALV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=392, Percent_Identity=30.1020408163265, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787181, Length=156, Percent_Identity=31.4102564102564, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43444; Mature: 43444

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKVILVSGKQNTVLRGHPWIFPKAIAQYSGKLITGELVEIIGSEGESLGFGAYNEHSL
CCEEEEEEECCCCCEEECCCCCCHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCCCCCCE
YRVRVLALSNEQINSFDYKSLITHRIKQASLVRRCLDLPNEQTNAYRLFNSEADGLSGLT
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCE
IDCFNDYCVIASSAYWVELNKSIIVHVLQELLPHNHIIWLPQNKPLSQDGWKEIHTEKSQ
EEECCCEEEEECCEEEEEECHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHCCCCC
GSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKLSRGKRVLDLYTFTGGFALHAAK
CHHHHHHCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHCCCEEEEEEEECCCEEEEECC
AGALHVTAVDSSVQAIEQAKNNAVLNHLNTIEFIKADAREYLKMAGEYDVVILDPPKLVP
CCEEEEEEECHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
SQKHLQQAKNYYRFLHREAFKYMKPGSLLMTCNCSSALSSQDFCSLVSAQAVAMGKQARI
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHCCCCEEE
LGVYGPASCHPTLAAFPEGNYLTAILLALV
EEEECCCCCCCCEEECCCCCHHHHHHHHHC
>Mature Secondary Structure
MKAKVILVSGKQNTVLRGHPWIFPKAIAQYSGKLITGELVEIIGSEGESLGFGAYNEHSL
CCEEEEEEECCCCCEEECCCCCCHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCCCCCCE
YRVRVLALSNEQINSFDYKSLITHRIKQASLVRRCLDLPNEQTNAYRLFNSEADGLSGLT
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCE
IDCFNDYCVIASSAYWVELNKSIIVHVLQELLPHNHIIWLPQNKPLSQDGWKEIHTEKSQ
EEECCCEEEEECCEEEEEECHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHCCCCC
GSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKLSRGKRVLDLYTFTGGFALHAAK
CHHHHHHCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHCCCEEEEEEEECCCEEEEECC
AGALHVTAVDSSVQAIEQAKNNAVLNHLNTIEFIKADAREYLKMAGEYDVVILDPPKLVP
CCEEEEEEECHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
SQKHLQQAKNYYRFLHREAFKYMKPGSLLMTCNCSSALSSQDFCSLVSAQAVAMGKQARI
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHCCCCEEE
LGVYGPASCHPTLAAFPEGNYLTAILLALV
EEEECCCCCCCCEEECCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA