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The map label for this gene is citH [H]

Identifier: 148358749

GI number: 148358749

Start: 1406171

End: 1407457

Strand: Direct

Name: citH [H]

Synonym: LPC_0628

Alternate gene names: 148358749

Gene position: 1406171-1407457 (Clockwise)

Preceding gene: 148358746

Following gene: 148358750

Centisome position: 39.32

GC content: 38.54

Gene sequence:

>1287_bases
ATGAATACATCAAATACCTCCTTTAAAACATTGGGAGCAATATGTATTGGTAATGCTATGGAATGGTATGATTTCATGAT
TTATAACTTCATGCTGCTTTTTATTGCCCAAGCTTTTTTTCCCAATCATAGTCCTGTGACTTCCCTCTTGGCTACAATGG
CAAGCTCCGGAGTGGCTCTGGTAGTCCGGCCTCTTGGTGGTTTTATTTTAGCAGTTTGGGCAGATAAGTATGGGCATCAT
AAAGCATTAATGTTCGTTTTATATTTGATGGCTGTCGCAACCATGCTTATTACTTTTACACCATCCTACTCCACAATAGG
TCTGGCGGCTACTTGTATCATTGTATTTGCAAGATTGTTGCAAGGGCTTGCTACTGGTGGCGAGTTTGGCATATCCAGCG
CTCTATTAGTTAGCTTATCGCCAAGTGATAAAAAGGGGTTTTACACTTCCTTACAAATGGTAGGTCAGCTTTTGGCAATA
TTAATGGGATCTTCAGCGTGCCTTATTTTAGTTTTCTATTGTTCCAATGAAACCCTATATCAATTTGCCTGGAGAATACC
TTTTGCTCTAGGACTGTTGATTCTACCTTTAGGATGGATTTTAAGACGTCGATTGCAACGAGAGTTTACTTGGAAGAAAG
AGCAACAGGACACAGGAAAGTTACTTATCTTGGTAAAGCAACAAAAACGTTCGCTGTTGATAGCTTTTAGTCTTGTAGCC
GGGTGTACAGGCTCGATTTATACCTTATTCAGCTATATGCCTACCTATTGTAAATTGTATTTGAATTTATCGCTGACAGA
GGCGTACTTAGGCACTGCATCAGGAATATTAGTCAGTATTCTTACTATTCCTCTTTTTGGCGCATTGTCGGATAAAATAG
GCAAAAAAATAGTTATGTTATGTGCTTTGGCCTGTTATTTTGGATTAATTTATCCTTTACTTTTCTTGCTCAACCAATAC
CCTGGTGTAACTATTCTCATTTTGGTTGAAAACATTTTAGGTTTGTGTATCGGCGCTTATTTTGGAGTGTTGACCATTAC
GGTTAGCTCCTTATTTCCACCATCAGTACGTTCTACTTGTTTAGCCGTCAGCTATAATTTAGCTGTCCTGTTGTTTGGTG
GTTTTGCTCCATTTATTATAACGGCATTGATTGAATACACTAAAAACCCTATGGTTTTTACTTATTATCAAATGACGACG
GTCTTTATCAGTTTGCTAGCAGTCCTTTCTTATCAGGAGGAAAGGAAGCTATCTGAGAATCAGCCAGAACATCTCGTTGC
AATTTAA

Upstream 100 bases:

>100_bases
AGTAAAATTAATATTGATGTTAGTTTCTTATAAAGCATGGTAATTTTTTTTATATTCTACTAGTATGTGCTTTGCTCTAA
ATTTACCCTCGTAAGGAAAC

Downstream 100 bases:

>100_bases
AAGGTAGGAATATGACAACTTTAGATTGGTTAAAACAACTCATTTCTTTTGATACTACTTCGCGGAACTCTAATTTATCA
TTGATTGAATATTTGGCGAA

Product: MFS transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVALVVRPLGGFILAVWADKYGHH
KALMFVLYLMAVATMLITFTPSYSTIGLAATCIIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSACLILVFYCSNETLYQFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDTGKLLILVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPLFGALSDKIGKKIVMLCALACYFGLIYPLLFLLNQY
PGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNLAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLSYQEERKLSENQPEHLVAI

Sequences:

>Translated_428_residues
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVALVVRPLGGFILAVWADKYGHH
KALMFVLYLMAVATMLITFTPSYSTIGLAATCIIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSACLILVFYCSNETLYQFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDTGKLLILVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPLFGALSDKIGKKIVMLCALACYFGLIYPLLFLLNQY
PGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNLAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLSYQEERKLSENQPEHLVAI
>Mature_428_residues
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVALVVRPLGGFILAVWADKYGHH
KALMFVLYLMAVATMLITFTPSYSTIGLAATCIIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSACLILVFYCSNETLYQFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDTGKLLILVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPLFGALSDKIGKKIVMLCALACYFGLIYPLLFLLNQY
PGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNLAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLSYQEERKLSENQPEHLVAI

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=28.6374133949192, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788942, Length=430, Percent_Identity=29.3023255813954, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788292, Length=433, Percent_Identity=29.3302540415704, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789941, Length=433, Percent_Identity=27.0207852193995, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI87082404, Length=369, Percent_Identity=24.9322493224932, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47210; Mature: 47210

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVAL
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
VVRPLGGFILAVWADKYGHHKALMFVLYLMAVATMLITFTPSYSTIGLAATCIIVFARLL
EEEHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAILMGSSACLILVFYCSNETLY
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHH
QFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDTGKLLILVKQQKRSLLIAFSLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPLFGALSDKIGKKIVML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CALACYFGLIYPLLFLLNQYPGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTC
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LAVSYNLAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTTVFISLLAVLSYQEERKLSEN
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
QPEHLVAI
CCCCCCCC
>Mature Secondary Structure
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVAL
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
VVRPLGGFILAVWADKYGHHKALMFVLYLMAVATMLITFTPSYSTIGLAATCIIVFARLL
EEEHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAILMGSSACLILVFYCSNETLY
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHH
QFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDTGKLLILVKQQKRSLLIAFSLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPLFGALSDKIGKKIVML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CALACYFGLIYPLLFLLNQYPGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTC
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LAVSYNLAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTTVFISLLAVLSYQEERKLSEN
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
QPEHLVAI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]