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The map label for this gene is artJ [H]

Identifier: 148358705

GI number: 148358705

Start: 1359329

End: 1360024

Strand: Direct

Name: artJ [H]

Synonym: LPC_0583

Alternate gene names: 148358705

Gene position: 1359329-1360024 (Clockwise)

Preceding gene: 148358702

Following gene: 148358706

Centisome position: 38.01

GC content: 34.91

Gene sequence:

>696_bases
ATGATTCTAAAGCCGGGAAAATTATTGGTGGCTACTGCTTATCCAGATCCACCGTTTGATATTGAGCCAGGTGATGGTTT
TGATGGTGATTTAATAAAAGAAATTTGTCGAATTTTAAATTTGCAGCTAGTCCAGATCAAATATGAGGGAGAAAATTTTA
ATGATATTTTTTTAGGCTTGGCTAATAAAGAATACGATGTTGTCATTTCAGGAACGACTATTACACCAAACCGATCAAAA
CAAGTACTTTTTTCGAATCCCTATTTAGAGTTTAATCAGGGTATCGCGGTTAATACTGAATTAAAGCCAAATGTTTCTTC
TTTTGAAGATTTGAAAGGTTTAGTGGCTGGTATACAGAAAGGCAACACATCAGATGAAGTTGCTCAGGATTTATTACGTA
AGAACATACTCAGTGAAATTAAATATTATGCATACAATGAAATAGAATTAGCCATACAGGATCTGTCTGCCGGTAAAATT
GGTTTGGTCATTAAATTATATCCTGTTATTTCCTGTTTAATTCGAAACTATACAAACCTGGCTGTTCCTTTGCAGGCTGA
AACACATGAAAAACTTGGTATCGCGGTTGCTAAAGATAATTTTAAACTCCGGGATAAAGTCAATCTTATTTTGGCCGATC
TTGTGCAAAATGGTACTTTAAGTCGCCTGCAACATAAGTGGATAAGTACAACCTAG

Upstream 100 bases:

>100_bases
GTAAATTATTCATTTAAATCAATGGCATAAAAAATATTTGGCTGATTTTTTTCTGATTAATCTATACTTAGTGAATCGAT
TCCAAACAATAGTTATATCT

Downstream 100 bases:

>100_bases
GGTAAATTAGGCCAATGTCAGTTTCAACTCAAGAAATAACCTTATTTAAGGAATCTTATTTTTCTTGAATCGTTCTGTAA
TTATTTTTCAAATCCTCTTG

Product: cystine/glutamine-binding periplasmic protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSK
QVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKNILSEIKYYAYNEIELAIQDLSAGKI
GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAVAKDNFKLRDKVNLILADLVQNGTLSRLQHKWISTT

Sequences:

>Translated_231_residues
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSK
QVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKNILSEIKYYAYNEIELAIQDLSAGKI
GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAVAKDNFKLRDKVNLILADLVQNGTLSRLQHKWISTT
>Mature_231_residues
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSK
QVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKNILSEIKYYAYNEIELAIQDLSAGKI
GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAVAKDNFKLRDKVNLILADLVQNGTLSRLQHKWISTT

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=221, Percent_Identity=32.1266968325792, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1787031, Length=232, Percent_Identity=31.4655172413793, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787088, Length=227, Percent_Identity=28.6343612334802, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788228, Length=231, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 25723; Mature: 25723

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGL
CCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEEECCCCCCEEEEEE
ANKEYDVVISGTTITPNRSKQVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQK
CCCCEEEEEECCEECCCCCCEEEEECCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHC
GNTSDEVAQDLLRKNILSEIKYYAYNEIELAIQDLSAGKIGLVIKLYPVISCLIRNYTNL
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEHHHHHHHHHHCCCCE
AVPLQAETHEKLGIAVAKDNFKLRDKVNLILADLVQNGTLSRLQHKWISTT
EEEEECCCCCCEEEEEECCCEEEHHHHHHHHHHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGL
CCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEEECCCCCCEEEEEE
ANKEYDVVISGTTITPNRSKQVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQK
CCCCEEEEEECCEECCCCCCEEEEECCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHC
GNTSDEVAQDLLRKNILSEIKYYAYNEIELAIQDLSAGKIGLVIKLYPVISCLIRNYTNL
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEHHHHHHHHHHCCCCE
AVPLQAETHEKLGIAVAKDNFKLRDKVNLILADLVQNGTLSRLQHKWISTT
EEEEECCCCCCEEEEEECCCEEEHHHHHHHHHHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]