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The map label for this gene is mltD [H]

Identifier: 148358702

GI number: 148358702

Start: 1356006

End: 1357334

Strand: Direct

Name: mltD [H]

Synonym: LPC_0580

Alternate gene names: 148358702

Gene position: 1356006-1357334 (Clockwise)

Preceding gene: 148358700

Following gene: 148358705

Centisome position: 37.91

GC content: 38.37

Gene sequence:

>1329_bases
TTGATTAAGACATTCATCGATAAAAAAATGCAGCTGCTATTCAGTTGCCTTATTTTTTTTATCTTCAGTACTCATGCTAA
ATCATTTGAAACACCTGATGTATGGGATGTTTTGCGCAGTGAATTCAAGATGAATCATGAAGCATATCGCCCAGAGGTTC
AAGATCAAATTCGCTGGCTACTGGATCATCCAGGGTTTGTGACTAAGGCCTGTAAACAGGCTGAGCCTTATCTTTATCAT
GTAACTCGAGAAATACAAAGAAGAAATTTACCCGGTGAGTTGGCATTACTCCCTATGATAGAAAGCGCTTACGATCCTTT
TGCTTACTCAAAAGTTGGTGCTGCAGGTTTATGGCAAATAATGCCGGTGACAGGGAATCAATTAGGTTTAATTCAGGATT
GGTGGTTTGATGGAAGACGAAGCATTAATCATTCAACTGATGCAGCCCTGACTCATCTGATGCATTTAAATAAAGTGTTT
AACGGAAACTGGATATTATCCATCGCAGCTTACGATGCTGGCGAAGGAGCCATAGGCAGAGCTGTAAAAGCAAATAACAC
TAATGGAGGTAGTGTCAGTTTTTGGAATCTGGATGTACCTAAAGAAACTCAAATTTATGTTCCTCGATTTTTAGCTCTGG
CTGAAATCATGAGCAACCCTCGGGCTTATAAAATAAATCTTCCTGCAATGCCATTTCGACCTTATTTTGAAGAGGTGAAT
ATTGGCAGTCAAATTGACTTGAATCATGCGGCTAGTTTAGCAGGAATTTCGGTTAGTGAATTAACCAAATTAAATCCGGG
TTATAATAGATGGGCTACCGCACCCTATAAACCCTTCACCTTGCTTATCCCTATAGCAAAAGTGACTCAATTTTATTTCA
ATTTATCCCATTCACCTGTTGATAAACGAGTGAGCTGGAGAAAACATCTCGTCCTTCCGAGTGATAGTTTACAAAGTATT
GCCAGAAAATATCATACGACTGTTTCATTAATAAAAAAATTAAATCGTCTGTCAGATCACTCGATAAGACCCAATCAGAT
CTTGTTAATTCCGAGCGCCAAAAGTACTCCATCATTGCCTTTCCACGAAATTTCAAAGTCAAAATTCTTAAGCCAGACAC
CAACAAAAGGTAAAGTATACAGGGTCATACATATTGTCCAAGCCAATGAAACGTATAATACCCTTGAGCGACTTTATAAA
GTTACAGCTCAGGATATTATGAAATGGAATCATATTGGCTCGGGAGAAGCGTTGGTAAAAGGGCGACAGTTAATTATTTG
GAAAAAGTCAAGCAATCTTGAATGGAACAGGACTTTCAAAAAACCTTAA

Upstream 100 bases:

>100_bases
GAGTATATTTTATAAGACGCGCTCAGGGCCGGTTCAGGCGAAGAGAAACAATACATTATAAACGGGCTCCAGAGAAGCTT
TATAGAGCAGGAATAGTAAT

Downstream 100 bases:

>100_bases
GTTTCCGGCAATCCTGCATTCTAAACATAACCCGTGTTCGGTCAAAATTTCACGCTTAACTATTCGATAACTTATTAATA
AATTTGGTTAAATTTCTCAT

Product: membrane bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MIKTFIDKKMQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKMNHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYH
VTREIQRRNLPGELALLPMIESAYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
NGNWILSIAAYDAGEGAIGRAVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMSNPRAYKINLPAMPFRPYFEEVN
IGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPYKPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSI
ARKYHTTVSLIKKLNRLSDHSIRPNQILLIPSAKSTPSLPFHEISKSKFLSQTPTKGKVYRVIHIVQANETYNTLERLYK
VTAQDIMKWNHIGSGEALVKGRQLIIWKKSSNLEWNRTFKKP

Sequences:

>Translated_442_residues
MIKTFIDKKMQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKMNHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYH
VTREIQRRNLPGELALLPMIESAYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
NGNWILSIAAYDAGEGAIGRAVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMSNPRAYKINLPAMPFRPYFEEVN
IGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPYKPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSI
ARKYHTTVSLIKKLNRLSDHSIRPNQILLIPSAKSTPSLPFHEISKSKFLSQTPTKGKVYRVIHIVQANETYNTLERLYK
VTAQDIMKWNHIGSGEALVKGRQLIIWKKSSNLEWNRTFKKP
>Mature_442_residues
MIKTFIDKKMQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKMNHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYH
VTREIQRRNLPGELALLPMIESAYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
NGNWILSIAAYDAGEGAIGRAVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMSNPRAYKINLPAMPFRPYFEEVN
IGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPYKPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSI
ARKYHTTVSLIKKLNRLSDHSIRPNQILLIPSAKSTPSLPFHEISKSKFLSQTPTKGKVYRVIHIVQANETYNTLERLYK
VTAQDIMKWNHIGSGEALVKGRQLIIWKKSSNLEWNRTFKKP

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=418, Percent_Identity=30.8612440191388, Blast_Score=227, Evalue=9e-61,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 50581; Mature: 50581

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTFIDKKMQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKMNHEAYRPEVQDQIRWL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCCCCHHHHHHHH
LDHPGFVTKACKQAEPYLYHVTREIQRRNLPGELALLPMIESAYDPFAYSKVGAAGLWQI
HCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCHHHHHCCCCCCEEE
MPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVFNGNWILSIAAYDAGEGAIGR
EECCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
AVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMSNPRAYKINLPAMPFRPYFEEVN
EEECCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCC
IGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPYKPFTLLIPIAKVTQFYFNLSHSPV
CCCEECCHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCH
DKRVSWRKHLVLPSDSLQSIARKYHTTVSLIKKLNRLSDHSIRPNQILLIPSAKSTPSLP
HHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
FHEISKSKFLSQTPTKGKVYRVIHIVQANETYNTLERLYKVTAQDIMKWNHIGSGEALVK
HHHHHHHHHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEC
GRQLIIWKKSSNLEWNRTFKKP
CCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MIKTFIDKKMQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKMNHEAYRPEVQDQIRWL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCCCCHHHHHHHH
LDHPGFVTKACKQAEPYLYHVTREIQRRNLPGELALLPMIESAYDPFAYSKVGAAGLWQI
HCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCHHHHHCCCCCCEEE
MPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVFNGNWILSIAAYDAGEGAIGR
EECCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
AVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMSNPRAYKINLPAMPFRPYFEEVN
EEECCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCC
IGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPYKPFTLLIPIAKVTQFYFNLSHSPV
CCCEECCHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCH
DKRVSWRKHLVLPSDSLQSIARKYHTTVSLIKKLNRLSDHSIRPNQILLIPSAKSTPSLP
HHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
FHEISKSKFLSQTPTKGKVYRVIHIVQANETYNTLERLYKVTAQDIMKWNHIGSGEALVK
HHHHHHHHHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEC
GRQLIIWKKSSNLEWNRTFKKP
CCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]