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The map label for this gene is rlmB [H]

Identifier: 148358250

GI number: 148358250

Start: 115201

End: 115950

Strand: Direct

Name: rlmB [H]

Synonym: LPC_0112

Alternate gene names: 148358250

Gene position: 115201-115950 (Clockwise)

Preceding gene: 148358249

Following gene: 148358251

Centisome position: 3.22

GC content: 41.6

Gene sequence:

>750_bases
ATGAATGAGCAGTATGTTTATGGGTTGCATGCAGTTTCTGCATTATTAGCAAACCCGCATCGAATGATCAAAAAACTCTT
TGTTAATCAAGATAGAATAGATAAAAGACTTCATGAGTTTTTGGTCAAGGCAGAAAACGCCCATGTCCCTATCGAAAAGC
TAAGCGCACAAAAAATGAATCAGCAGTTTTCAGAGTTTACGCATCAGGGCGTTGTTGCACTCGCTTCGGCATTACCCGAA
TACGGTGAAAGTGATTTGGTTACTTTATTAGAAAGAAGTAAAAAGCCTGTGTTGATTCTTATTCTGGATGGTATTACAGA
TCCCCATAATTTAGGCGCTTGTTTGCGGTCGGCTGATGCAACCGGTGTTGATTTTGTTGTGATTCCTAAAGATAAAAGTG
CGAATATTACTCCCGTGGTCAGTAAAGTGGCTTGCGGGGCTGCTGAATCAATTCCTTTGGTGAGAGTAACTAATTTAGCA
AGAGCTATGGACATCTTAAAACAACAGGGTGTCTGGATCTTTGGAGCAGCTGGCGAAGCAGAGCATTTACTTTATGCAAT
GGATTGTTCTGGAAACATCGCTCTTGTAATGGGAGCAGAAGGAACCGGGTTAAGAAGATTGACTCGCGAGCATTGCGATG
GATTGTTTTCTTTGCCTATGATGGGAAGTGTAGAGAGTTTAAATGTGTCAGTGGCGACTGGTGTTTCATTGTATGAAGTA
ATTAGGCAAAGAGGGCAATTTCGTGAGTGA

Upstream 100 bases:

>100_bases
CAGGTTTATCGTTTGGGAGATAAAATGACTGTGTTGGTAGCTCGTGTTGATTTAAACGAACGTAAAATAGATTTTGAGCC
TGTCGAGGATGAAGTTTGTA

Downstream 100 bases:

>100_bases
ATTAAAAATCAAAGCAGCCAAAGCAGCTATAGCTTATATTGAAGATGATATGGTTATAGGTGTTGGAACAGGCTCTACAG
TTAATTTTTTTATAAAAGAA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNEQYVYGLHAVSALLANPHRMIKKLFVNQDRIDKRLHEFLVKAENAHVPIEKLSAQKMNQQFSEFTHQGVVALASALPE
YGESDLVTLLERSKKPVLILILDGITDPHNLGACLRSADATGVDFVVIPKDKSANITPVVSKVACGAAESIPLVRVTNLA
RAMDILKQQGVWIFGAAGEAEHLLYAMDCSGNIALVMGAEGTGLRRLTREHCDGLFSLPMMGSVESLNVSVATGVSLYEV
IRQRGQFRE

Sequences:

>Translated_249_residues
MNEQYVYGLHAVSALLANPHRMIKKLFVNQDRIDKRLHEFLVKAENAHVPIEKLSAQKMNQQFSEFTHQGVVALASALPE
YGESDLVTLLERSKKPVLILILDGITDPHNLGACLRSADATGVDFVVIPKDKSANITPVVSKVACGAAESIPLVRVTNLA
RAMDILKQQGVWIFGAAGEAEHLLYAMDCSGNIALVMGAEGTGLRRLTREHCDGLFSLPMMGSVESLNVSVATGVSLYEV
IRQRGQFRE
>Mature_249_residues
MNEQYVYGLHAVSALLANPHRMIKKLFVNQDRIDKRLHEFLVKAENAHVPIEKLSAQKMNQQFSEFTHQGVVALASALPE
YGESDLVTLLERSKKPVLILILDGITDPHNLGACLRSADATGVDFVVIPKDKSANITPVVSKVACGAAESIPLVRVTNLA
RAMDILKQQGVWIFGAAGEAEHLLYAMDCSGNIALVMGAEGTGLRRLTREHCDGLFSLPMMGSVESLNVSVATGVSLYEV
IRQRGQFRE

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=261, Percent_Identity=29.8850574712644, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=52.6970954356847, Blast_Score=260, Evalue=5e-71,
Organism=Escherichia coli, GI1788935, Length=244, Percent_Identity=25.8196721311475, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790083, Length=162, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17555908, Length=259, Percent_Identity=27.4131274131274, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324775, Length=292, Percent_Identity=28.4246575342466, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27193; Mature: 27193

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQYVYGLHAVSALLANPHRMIKKLFVNQDRIDKRLHEFLVKAENAHVPIEKLSAQKMN
CCCCEEHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
QQFSEFTHQGVVALASALPEYGESDLVTLLERSKKPVLILILDGITDPHNLGACLRSADA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCC
TGVDFVVIPKDKSANITPVVSKVACGAAESIPLVRVTNLARAMDILKQQGVWIFGAAGEA
CCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
EHLLYAMDCSGNIALVMGAEGTGLRRLTREHCDGLFSLPMMGSVESLNVSVATGVSLYEV
CEEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCEECCCCCCCHHHCEEEHHHCHHHHHH
IRQRGQFRE
HHHHCCCCC
>Mature Secondary Structure
MNEQYVYGLHAVSALLANPHRMIKKLFVNQDRIDKRLHEFLVKAENAHVPIEKLSAQKMN
CCCCEEHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
QQFSEFTHQGVVALASALPEYGESDLVTLLERSKKPVLILILDGITDPHNLGACLRSADA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCC
TGVDFVVIPKDKSANITPVVSKVACGAAESIPLVRVTNLARAMDILKQQGVWIFGAAGEA
CCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
EHLLYAMDCSGNIALVMGAEGTGLRRLTREHCDGLFSLPMMGSVESLNVSVATGVSLYEV
CEEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCEECCCCCCCHHHCEEEHHHCHHHHHH
IRQRGQFRE
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]