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The map label for this gene is vacB [H]

Identifier: 148358249

GI number: 148358249

Start: 113109

End: 115208

Strand: Direct

Name: vacB [H]

Synonym: LPC_0111

Alternate gene names: 148358249

Gene position: 113109-115208 (Clockwise)

Preceding gene: 148358248

Following gene: 148358250

Centisome position: 3.16

GC content: 43.05

Gene sequence:

>2100_bases
ATGGAAATCCTGGATGAATACGGTAGGCCAATGTCTCGCAGTAAACTGTTTGATAAATTGGATTTGAGCTCTGAGAGCCA
GCAGGAATCCTTGGGTTTTCGCTTGAAGGCCATGCTGCGTGATGGCCAAATCATGCAGGACAGGCGTGGACGCTTTTGTT
TATTGCAAAAAATTAATTTATTGCGTGGAACGGTTCAAGGCCATCCTGATGGTTTTGGATTTTTCATCCCGGATGATGGT
TCTGAGGATATGGTGCTCTCTGCTAAAGAAATGAGGGCTGTCATGCATGGAGATATGGTGCTTGCCTATCAATCTGGATT
GGATAGAAGAGGAAGGCCAGAAGCTAAAATCCACGAAGTCCTGGAACATGCGAATACCACGGTTGTAGGTCGATTTTTTA
CTGATCATGGCGTGAATTTTGTCATTCCTGATAGCAAACGTCTGACTCAGGATATTTCAATTCCCCAAGAATTCATTGGC
GAGGCCAAAAATGGCCAAATAGTACTGGTTGAGTTAATCGCATACCCTAACAAGCGTTCGCAAGCTATAGGCAGAGTAAT
CCATATTTTGGGTGAGCATATGGCTCCTGGCATGGAAATTCAGGTGGCAATCCATGCCCACGGCATTCCTTTCGAATGGC
CAGATGATGTTCTTGCGGAAGTGGGTAAAATTCCACAACAGATTTCCGAGGAACAATTAAAGGGTCGAACCGATTTGCGT
AATCTTCCTTTTGTAACCATTGACGGGGAAGATGCAAAAGATTTTGATGATGCTGTGTATTGTTACAAAAAGCCCAAAGG
CGGCTTCCAATTGTATGTCGCTATCGCAGACGTCAGTCATTATGTGGCGAAGGATTCTGCTTTGGATCAAGAAGCAGCAA
GGCGAGGAAATTCAGTTTATTTTCCCGGTAAAGTGGTGCCCATGCTACCAGAGGCCTTATCCAATGGCGTTTGCTCATTA
AATCCTCATGTTGACCGCTTATGTATGGTGGCTGAGTTATCGATTACCCAAGAAGGAAAAATATCCCGATCCCGCTTTTA
TCGGGCAGTGATTCATTCTCATGCTCGTTTGACCTACACGTCAGTTGGGGCATGGTTGGAACAAGGCAAGGCAGATGAAA
AACATCAAGCCTTGTGGCCTATGCTTGAGTCTCTTTATGATTTATATCATGTTCTGCTCGATACCCGTAGACTTAGAGGT
GCTATGGATTTCGAGACTACGGAAACTCGTATCGAATTTGATGAAAATAAGAAAATAAAATGTATCGTGCCTGTAATTCG
TAATGACGCTCATAAATTAATTGAAGAATGCATGCTGGCAGCGAATGTTGCGACAGCAAGATTTTTAGAAAAGGCTGAAA
TTCCTACTTTATACCGAGTTCATGCGGCTCCTGAAGAGGATAAAATTACTGCATTGAGGCAGTTTTTAGGGGAGCTGGGA
TTGTCATTGGGAGGGGGGAGAAAACCTGGACCCAAAGATTTTCAGCGCACCATGAACATGATTGGTGACCGACCAGAGAA
GCATCTTATTGAAACGGTAATGCTGCGTTCTTTAAAACAGGCTCAGTACGTTGAATCGAATGAAGGTCATTTTGGTTTGG
CCTATTCCGCTTACACGCATTTTACATCACCAATCAGAAGATTTCCTGATCTATTAATTCACAGAGCAATAGGGCATTTA
CTGGATAATCATCCTGTTTATGAGTTTGAATATAGCCATGAAGATATGAATCGTTTGGGTAAACATTCCTCGATGACGGA
GCGCCGTGCTGATGAAGCGACCCGGGAAGTGATTACCTGGTTAAAGTGCGAGTATATGCAAGACAAATTGGGGCAGGTAT
TTAAGGGTCGAATATCCGCTGTGACCAGCTTTGGTATTTTTGTGGAGCTTGATGAAATTTATGTAGAAGGCTTGGTTCAT
GTCACTTCTTTAAAAAACGATTATTATACGTTTGACTCTGCAAAACATCGTTTAATAGGGGCTCGTGGCGGTCAGGTTTA
TCGTTTGGGAGATAAAATGACTGTGTTGGTAGCTCGTGTTGATTTAAACGAACGTAAAATAGATTTTGAGCCTGTCGAGG
ATGAAGTTTGTAATGAATGA

Upstream 100 bases:

>100_bases
AAGAGAAAAATGGTGAAAAGTGAGTAAAAAATCCAAAGATCCCTTTTATAAGAGGGAGCGCGAAAAATACGCCGAGCCAA
TTCCAAGCCGGGAATACATC

Downstream 100 bases:

>100_bases
GCAGTATGTTTATGGGTTGCATGCAGTTTCTGCATTATTAGCAAACCCGCATCGAATGATCAAAAAACTCTTTGTTAATC
AAGATAGAATAGATAAAAGA

Product: (exo)ribonuclease R

Products: NA

Alternate protein names: RNase R [H]

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MEILDEYGRPMSRSKLFDKLDLSSESQQESLGFRLKAMLRDGQIMQDRRGRFCLLQKINLLRGTVQGHPDGFGFFIPDDG
SEDMVLSAKEMRAVMHGDMVLAYQSGLDRRGRPEAKIHEVLEHANTTVVGRFFTDHGVNFVIPDSKRLTQDISIPQEFIG
EAKNGQIVLVELIAYPNKRSQAIGRVIHILGEHMAPGMEIQVAIHAHGIPFEWPDDVLAEVGKIPQQISEEQLKGRTDLR
NLPFVTIDGEDAKDFDDAVYCYKKPKGGFQLYVAIADVSHYVAKDSALDQEAARRGNSVYFPGKVVPMLPEALSNGVCSL
NPHVDRLCMVAELSITQEGKISRSRFYRAVIHSHARLTYTSVGAWLEQGKADEKHQALWPMLESLYDLYHVLLDTRRLRG
AMDFETTETRIEFDENKKIKCIVPVIRNDAHKLIEECMLAANVATARFLEKAEIPTLYRVHAAPEEDKITALRQFLGELG
LSLGGGRKPGPKDFQRTMNMIGDRPEKHLIETVMLRSLKQAQYVESNEGHFGLAYSAYTHFTSPIRRFPDLLIHRAIGHL
LDNHPVYEFEYSHEDMNRLGKHSSMTERRADEATREVITWLKCEYMQDKLGQVFKGRISAVTSFGIFVELDEIYVEGLVH
VTSLKNDYYTFDSAKHRLIGARGGQVYRLGDKMTVLVARVDLNERKIDFEPVEDEVCNE

Sequences:

>Translated_699_residues
MEILDEYGRPMSRSKLFDKLDLSSESQQESLGFRLKAMLRDGQIMQDRRGRFCLLQKINLLRGTVQGHPDGFGFFIPDDG
SEDMVLSAKEMRAVMHGDMVLAYQSGLDRRGRPEAKIHEVLEHANTTVVGRFFTDHGVNFVIPDSKRLTQDISIPQEFIG
EAKNGQIVLVELIAYPNKRSQAIGRVIHILGEHMAPGMEIQVAIHAHGIPFEWPDDVLAEVGKIPQQISEEQLKGRTDLR
NLPFVTIDGEDAKDFDDAVYCYKKPKGGFQLYVAIADVSHYVAKDSALDQEAARRGNSVYFPGKVVPMLPEALSNGVCSL
NPHVDRLCMVAELSITQEGKISRSRFYRAVIHSHARLTYTSVGAWLEQGKADEKHQALWPMLESLYDLYHVLLDTRRLRG
AMDFETTETRIEFDENKKIKCIVPVIRNDAHKLIEECMLAANVATARFLEKAEIPTLYRVHAAPEEDKITALRQFLGELG
LSLGGGRKPGPKDFQRTMNMIGDRPEKHLIETVMLRSLKQAQYVESNEGHFGLAYSAYTHFTSPIRRFPDLLIHRAIGHL
LDNHPVYEFEYSHEDMNRLGKHSSMTERRADEATREVITWLKCEYMQDKLGQVFKGRISAVTSFGIFVELDEIYVEGLVH
VTSLKNDYYTFDSAKHRLIGARGGQVYRLGDKMTVLVARVDLNERKIDFEPVEDEVCNE
>Mature_699_residues
MEILDEYGRPMSRSKLFDKLDLSSESQQESLGFRLKAMLRDGQIMQDRRGRFCLLQKINLLRGTVQGHPDGFGFFIPDDG
SEDMVLSAKEMRAVMHGDMVLAYQSGLDRRGRPEAKIHEVLEHANTTVVGRFFTDHGVNFVIPDSKRLTQDISIPQEFIG
EAKNGQIVLVELIAYPNKRSQAIGRVIHILGEHMAPGMEIQVAIHAHGIPFEWPDDVLAEVGKIPQQISEEQLKGRTDLR
NLPFVTIDGEDAKDFDDAVYCYKKPKGGFQLYVAIADVSHYVAKDSALDQEAARRGNSVYFPGKVVPMLPEALSNGVCSL
NPHVDRLCMVAELSITQEGKISRSRFYRAVIHSHARLTYTSVGAWLEQGKADEKHQALWPMLESLYDLYHVLLDTRRLRG
AMDFETTETRIEFDENKKIKCIVPVIRNDAHKLIEECMLAANVATARFLEKAEIPTLYRVHAAPEEDKITALRQFLGELG
LSLGGGRKPGPKDFQRTMNMIGDRPEKHLIETVMLRSLKQAQYVESNEGHFGLAYSAYTHFTSPIRRFPDLLIHRAIGHL
LDNHPVYEFEYSHEDMNRLGKHSSMTERRADEATREVITWLKCEYMQDKLGQVFKGRISAVTSFGIFVELDEIYVEGLVH
VTSLKNDYYTFDSAKHRLIGARGGQVYRLGDKMTVLVARVDLNERKIDFEPVEDEVCNE

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014623, Length=409, Percent_Identity=29.5843520782396, Blast_Score=178, Evalue=2e-44,
Organism=Homo sapiens, GI190014625, Length=409, Percent_Identity=29.5843520782396, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI134288890, Length=441, Percent_Identity=29.9319727891156, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI219521928, Length=538, Percent_Identity=24.3494423791822, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI19115966, Length=538, Percent_Identity=24.3494423791822, Blast_Score=146, Evalue=7e-35,
Organism=Homo sapiens, GI156105695, Length=210, Percent_Identity=29.5238095238095, Blast_Score=72, Evalue=3e-12,
Organism=Homo sapiens, GI156105693, Length=210, Percent_Identity=29.5238095238095, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI87082383, Length=705, Percent_Identity=49.7872340425532, Blast_Score=720, Evalue=0.0,
Organism=Escherichia coli, GI1787542, Length=648, Percent_Identity=26.3888888888889, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI212645896, Length=566, Percent_Identity=25.9717314487633, Blast_Score=168, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17553506, Length=468, Percent_Identity=29.0598290598291, Blast_Score=166, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324552, Length=624, Percent_Identity=25.3205128205128, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24649634, Length=413, Percent_Identity=29.7820823244552, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19922976, Length=372, Percent_Identity=30.1075268817204, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24654597, Length=372, Percent_Identity=30.1075268817204, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24654592, Length=372, Percent_Identity=30.1075268817204, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- InterPro:   IPR013668 [H]

Pfam domain/function: PF08461 HTH_12; PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 79379; Mature: 79379

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEILDEYGRPMSRSKLFDKLDLSSESQQESLGFRLKAMLRDGQIMQDRRGRFCLLQKINL
CCCHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCEEHHHHHHH
LRGTVQGHPDGFGFFIPDDGSEDMVLSAKEMRAVMHGDMVLAYQSGLDRRGRPEAKIHEV
HHHHCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHH
LEHANTTVVGRFFTDHGVNFVIPDSKRLTQDISIPQEFIGEAKNGQIVLVELIAYPNKRS
HHHCCCEEEEEEHHCCCCEEEECCCHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCCHHH
QAIGRVIHILGEHMAPGMEIQVAIHAHGIPFEWPDDVLAEVGKIPQQISEEQLKGRTDLR
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
NLPFVTIDGEDAKDFDDAVYCYKKPKGGFQLYVAIADVSHYVAKDSALDQEAARRGNSVY
CCCEEEECCCCCCCCCCCEEEEECCCCCEEEEEEEEHHHHHHHHHHCCCHHHHHCCCCEE
FPGKVVPMLPEALSNGVCSLNPHVDRLCMVAELSITQEGKISRSRFYRAVIHSHARLTYT
ECCCHHHHCHHHHCCCCEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEHH
SVGAWLEQGKADEKHQALWPMLESLYDLYHVLLDTRRLRGAMDFETTETRIEFDENKKIK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEE
CIVPVIRNDAHKLIEECMLAANVATARFLEKAEIPTLYRVHAAPEEDKITALRQFLGELG
EEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHC
LSLGGGRKPGPKDFQRTMNMIGDRPEKHLIETVMLRSLKQAQYVESNEGHFGLAYSAYTH
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
FTSPIRRFPDLLIHRAIGHLLDNHPVYEFEYSHEDMNRLGKHSSMTERRADEATREVITW
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKCEYMQDKLGQVFKGRISAVTSFGIFVELDEIYVEGLVHVTSLKNDYYTFDSAKHRLIG
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHEECCCCCCEECCCCCHHEEE
ARGGQVYRLGDKMTVLVARVDLNERKIDFEPVEDEVCNE
CCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MEILDEYGRPMSRSKLFDKLDLSSESQQESLGFRLKAMLRDGQIMQDRRGRFCLLQKINL
CCCHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCEEHHHHHHH
LRGTVQGHPDGFGFFIPDDGSEDMVLSAKEMRAVMHGDMVLAYQSGLDRRGRPEAKIHEV
HHHHCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHH
LEHANTTVVGRFFTDHGVNFVIPDSKRLTQDISIPQEFIGEAKNGQIVLVELIAYPNKRS
HHHCCCEEEEEEHHCCCCEEEECCCHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCCHHH
QAIGRVIHILGEHMAPGMEIQVAIHAHGIPFEWPDDVLAEVGKIPQQISEEQLKGRTDLR
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
NLPFVTIDGEDAKDFDDAVYCYKKPKGGFQLYVAIADVSHYVAKDSALDQEAARRGNSVY
CCCEEEECCCCCCCCCCCEEEEECCCCCEEEEEEEEHHHHHHHHHHCCCHHHHHCCCCEE
FPGKVVPMLPEALSNGVCSLNPHVDRLCMVAELSITQEGKISRSRFYRAVIHSHARLTYT
ECCCHHHHCHHHHCCCCEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEHH
SVGAWLEQGKADEKHQALWPMLESLYDLYHVLLDTRRLRGAMDFETTETRIEFDENKKIK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEE
CIVPVIRNDAHKLIEECMLAANVATARFLEKAEIPTLYRVHAAPEEDKITALRQFLGELG
EEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHC
LSLGGGRKPGPKDFQRTMNMIGDRPEKHLIETVMLRSLKQAQYVESNEGHFGLAYSAYTH
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
FTSPIRRFPDLLIHRAIGHLLDNHPVYEFEYSHEDMNRLGKHSSMTERRADEATREVITW
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKCEYMQDKLGQVFKGRISAVTSFGIFVELDEIYVEGLVHVTSLKNDYYTFDSAKHRLIG
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHEECCCCCCEECCCCCHHEEE
ARGGQVYRLGDKMTVLVARVDLNERKIDFEPVEDEVCNE
CCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]