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The map label for this gene is tilS

Identifier: 148285169

GI number: 148285169

Start: 2112725

End: 2114050

Strand: Direct

Name: tilS

Synonym: OTBS_2167

Alternate gene names: 148285169

Gene position: 2112725-2114050 (Clockwise)

Preceding gene: 148285168

Following gene: 148285170

Centisome position: 99.33

GC content: 29.79

Gene sequence:

>1326_bases
ATGAATATTGAGGTGAATTTTTGTCATAATATGGAGCATTTTGGAGCTTTTGAGGAGAATCCAAAGCTCGCTATAGCTGT
CTCTGGAGGAACTGACTCATTGGCTTTAATGTTACTAGTGAAACACTGGAACGAGAAGGTAAAAGGAGAAATAACTGTTT
TAACAATAGATCATCACTTGCGTTCTGAATCAACTAGCGAAGCAGATTATGTTAGTAGTATATGTCAGAATATAAAACTT
CAGCATGTTACTCTACACTGGATACATAAAGGAATTACAGGAAATATTCAAGCTCAGGCTCGTAAAGCCAGATATCATTT
GTTAACTAACTATTGTCAAGAGCATGACATTTTACATTTGATTACAGGACATCATGCAGATGATATAGTTGAAAATTTTT
TCATAAGATTACTTCGTGGAGCAGGACTAGCTGGATTGAGTTCTCATAATATTTTCTTTGTTAATAATGTTCGAATTATT
CGTCCACTATTTAATATTACTAAACAAGATTTAAAAAAATATTTAGAACAGCAGAATATTAAATGGATAAATGATCCATC
TAATAACTCAAATAAGTATCTTCGTACTCAAGTTCGGGATTTATTAAAATCTATGCTTATTTCTTTTCAGAATAATTTTA
CAGTAGAATTACTAAAAAAAAGAATAATGCTTAGCCAAATGCATTTAACACGAGCTTTAGACAGTGTAAATAATGAAATA
ATTCACTATGTAGTTTATGCAGTTAAAATTTATTCAGCCGGTTTTGCTGTAATCGATAGAAAGCTTTTTAGGCAAGCTTC
ACCTGAGGCTCGATATGCTATATTATCATACTTACTGATGATTGTTGGTGCTAATACAAAACCTCAAAGATTTTCTTCTT
TACAACATATTATTTTGCATGATATACAAGAGTATAATACTTATAAAACTTTACATGGATGCATTGTTGAATATTCTATA
CAATATATTATAATATATAGAGAGTTTGGGCGTTGCTATCCTAGATCAAAGGTTGTAAGTAATTCAGTAGTTTGGGACTA
TAGATTTAAAGTAGTTGATAATAGAAAAAATAATCGTATGAATTTAACTATTGATTATTTAAAAAAAAGCGACTATCACT
TAATTAAATCCTATGTTGAAAGTAATCAGAGAAATGCTTACTTTAACTACAGCAGGAAAATATTATTTACTTTTCCTGTG
ATTAAACATCTTGAAAAAGTCATAGCGATTCCACATATAAAATACTATAGTGATAAAACAATTCAAGAAAGTGTGAGCTT
TGTTTTTGAACCGAAGTTAATATCGCGTTGGTTTCATTATTGTTAA

Upstream 100 bases:

>100_bases
AAGCGTTATTGTATAATGCTTAGTCAAAATGAGATATGGATTATTCAAGTTTGAAACTATTGATAGTATGCAGACAGAAG
GGTTTAAAGCTATTTAATCA

Downstream 100 bases:

>100_bases
AAAATTTAGGGTTAATATGAATAATCAAAACAGAAATGTATTATTTTGGGCAACAGTATTTATTGTACTTGTTGTAGCAT
TTAATTTGCTGCAAGGAGAA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNIEVNFCHNMEHFGAFEENPKLAIAVSGGTDSLALMLLVKHWNEKVKGEITVLTIDHHLRSESTSEADYVSSICQNIKL
QHVTLHWIHKGITGNIQAQARKARYHLLTNYCQEHDILHLITGHHADDIVENFFIRLLRGAGLAGLSSHNIFFVNNVRII
RPLFNITKQDLKKYLEQQNIKWINDPSNNSNKYLRTQVRDLLKSMLISFQNNFTVELLKKRIMLSQMHLTRALDSVNNEI
IHYVVYAVKIYSAGFAVIDRKLFRQASPEARYAILSYLLMIVGANTKPQRFSSLQHIILHDIQEYNTYKTLHGCIVEYSI
QYIIIYREFGRCYPRSKVVSNSVVWDYRFKVVDNRKNNRMNLTIDYLKKSDYHLIKSYVESNQRNAYFNYSRKILFTFPV
IKHLEKVIAIPHIKYYSDKTIQESVSFVFEPKLISRWFHYC

Sequences:

>Translated_441_residues
MNIEVNFCHNMEHFGAFEENPKLAIAVSGGTDSLALMLLVKHWNEKVKGEITVLTIDHHLRSESTSEADYVSSICQNIKL
QHVTLHWIHKGITGNIQAQARKARYHLLTNYCQEHDILHLITGHHADDIVENFFIRLLRGAGLAGLSSHNIFFVNNVRII
RPLFNITKQDLKKYLEQQNIKWINDPSNNSNKYLRTQVRDLLKSMLISFQNNFTVELLKKRIMLSQMHLTRALDSVNNEI
IHYVVYAVKIYSAGFAVIDRKLFRQASPEARYAILSYLLMIVGANTKPQRFSSLQHIILHDIQEYNTYKTLHGCIVEYSI
QYIIIYREFGRCYPRSKVVSNSVVWDYRFKVVDNRKNNRMNLTIDYLKKSDYHLIKSYVESNQRNAYFNYSRKILFTFPV
IKHLEKVIAIPHIKYYSDKTIQESVSFVFEPKLISRWFHYC
>Mature_441_residues
MNIEVNFCHNMEHFGAFEENPKLAIAVSGGTDSLALMLLVKHWNEKVKGEITVLTIDHHLRSESTSEADYVSSICQNIKL
QHVTLHWIHKGITGNIQAQARKARYHLLTNYCQEHDILHLITGHHADDIVENFFIRLLRGAGLAGLSSHNIFFVNNVRII
RPLFNITKQDLKKYLEQQNIKWINDPSNNSNKYLRTQVRDLLKSMLISFQNNFTVELLKKRIMLSQMHLTRALDSVNNEI
IHYVVYAVKIYSAGFAVIDRKLFRQASPEARYAILSYLLMIVGANTKPQRFSSLQHIILHDIQEYNTYKTLHGCIVEYSI
QYIIIYREFGRCYPRSKVVSNSVVWDYRFKVVDNRKNNRMNLTIDYLKKSDYHLIKSYVESNQRNAYFNYSRKILFTFPV
IKHLEKVIAIPHIKYYSDKTIQESVSFVFEPKLISRWFHYC

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=190, Percent_Identity=30, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_ORITB (A5CFP2)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001249259.1
- STRING:   A5CFP2
- GeneID:   5219209
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_2167
- eggNOG:   COG0037
- HOGENOM:   HBG586536
- OMA:   NIQIIRP
- ProtClustDB:   CLSK2502390
- BioCyc:   OTSU357244:OTBS_2167-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 51768; Mature: 51768

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEVNFCHNMEHFGAFEENPKLAIAVSGGTDSLALMLLVKHWNEKVKGEITVLTIDHHL
CCEEEEEECCHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCHHHCCEEEEEEEECHH
RSESTSEADYVSSICQNIKLQHVTLHWIHKGITGNIQAQARKARYHLLTNYCQEHDILHL
CCCCCCHHHHHHHHHHCCEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
ITGHHADDIVENFFIRLLRGAGLAGLSSHNIFFVNNVRIIRPLFNITKQDLKKYLEQQNI
ECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
KWINDPSNNSNKYLRTQVRDLLKSMLISFQNNFTVELLKKRIMLSQMHLTRALDSVNNEI
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHYVVYAVKIYSAGFAVIDRKLFRQASPEARYAILSYLLMIVGANTKPQRFSSLQHIILH
HHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DIQEYNTYKTLHGCIVEYSIQYIIIYREFGRCYPRSKVVSNSVVWDYRFKVVDNRKNNRM
HHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCHHHCCCEEEEEEEEEEECCCCCEE
NLTIDYLKKSDYHLIKSYVESNQRNAYFNYSRKILFTFPVIKHLEKVIAIPHIKYYSDKT
EEEEEECCCCCHHHHHHHHHCCCCCEEEECCCEEEEEHHHHHHHHHHHHCCCEEECCCCH
IQESVSFVFEPKLISRWFHYC
HHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MNIEVNFCHNMEHFGAFEENPKLAIAVSGGTDSLALMLLVKHWNEKVKGEITVLTIDHHL
CCEEEEEECCHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCHHHCCEEEEEEEECHH
RSESTSEADYVSSICQNIKLQHVTLHWIHKGITGNIQAQARKARYHLLTNYCQEHDILHL
CCCCCCHHHHHHHHHHCCEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
ITGHHADDIVENFFIRLLRGAGLAGLSSHNIFFVNNVRIIRPLFNITKQDLKKYLEQQNI
ECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
KWINDPSNNSNKYLRTQVRDLLKSMLISFQNNFTVELLKKRIMLSQMHLTRALDSVNNEI
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHYVVYAVKIYSAGFAVIDRKLFRQASPEARYAILSYLLMIVGANTKPQRFSSLQHIILH
HHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DIQEYNTYKTLHGCIVEYSIQYIIIYREFGRCYPRSKVVSNSVVWDYRFKVVDNRKNNRM
HHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCHHHCCCEEEEEEEEEEECCCCCEE
NLTIDYLKKSDYHLIKSYVESNQRNAYFNYSRKILFTFPVIKHLEKVIAIPHIKYYSDKT
EEEEEECCCCCHHHHHHHHHCCCCCEEEECCCEEEEEHHHHHHHHHHHHCCCEEECCCCH
IQESVSFVFEPKLISRWFHYC
HHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA