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The map label for this gene is nuoM [H]

Identifier: 148285167

GI number: 148285167

Start: 2109711

End: 2111159

Strand: Direct

Name: nuoM [H]

Synonym: OTBS_2165

Alternate gene names: 148285167

Gene position: 2109711-2111159 (Clockwise)

Preceding gene: 148285166

Following gene: 148285168

Centisome position: 99.18

GC content: 29.4

Gene sequence:

>1449_bases
TTGCCATTAAGTGCATTGATAATTTTGTTGCTTATGAGATTAAGCAATTATTTTAATAAATTAGTGTATGTTAAATTCAT
TGCACTATTAAGTTCATTATTAACATTACTTTGTACCATATATTTATTATTACAATTTGATAATACTGCACATACATATC
AGTTTGTTGAATTATATTCAATATTTAAATTGATTGGGCTTAATTATCATGTAGGTATTGATGGTATATCTATCTTCTTT
ATTGCTTTAACAGCTTTACTTACATTATTATCTATTGTTATTAGTATTTTTACTGTTCAGAAAAAACTGGAAGATAATTT
ATTATGTTTTTTATTAATAGAATCATTAGTTATCGGAGCCTTTAGCTCGATGAATTTATTGCTATTTTTCATGTTTTTTG
AAGCATCATTGTTTCCAATATTTTTACTAATAGGAATTTGGGGAAGCAGCAATAGAATCTATGCTGCATTAAAGTTTTTT
CTGTATAGTTTTGTTGGATCAGTATTTTTCTTGGTAGCTATTATATATATACACACATACACTGGTACACTTGAGATTAC
AGAGTTATATGATCTTATGCCTAGGTTTGATATATCAGTACAAATGCTATTATGGTTAAGTATATTTGTTGCTTTTGCTA
TTAAAATACCTATGTTGCCTTTGCATAGTTGGTTGCCTGATGCTCACGTTGAAGCCCAAACTGGAGGTTCTGTAATGCTA
GCTGGAATATTACTTAAATTGGGTGGATTTGGATTATTAAGAATATGTTTGCCTATGTTGCCAGCTGCATCACAAAAATT
TAGTAGTATCGCCATGATATTCAGTGTGGCTGCTATTATTTATTTTTCCATTATAGCTTTTCAACAAATAGATATAAAAA
AAGCAATTGCTTATTCTTCAATTGCTCATATGGGGTATGTTACTGTTGGTATTTTTAGCTTGAATACTTATGGAATTCAG
GGAGCAATTTTTCAAATGTTAAGTCATGGATTGATATCACCAGCTTTATTTATGATTATTGGTATTTTATATGATCGAAC
TCATACTAGAGAAATAAGGTTTTATGGTGGTTTAGCTGTTAAAATGACTAAATTAGCTAGTATTTTTATGGCAGCAGTAC
TTGGGTCAATAGGTGTGCCAGGTACAAGTGGATTTATTGGTGAATTTTTAGTGTTATTAGGGGCTATTACCGCATCTCCA
TTAATTGGAAGTTTTGCAATTCTAGGAGTGTTACTTGGGGCTGTATATATGCTTTCGCTTTATCGGCGTATTATGTTAGG
AGAAATTACTAATCAAAGAGTTTATCAGTTGAATGATATTTTATTAAGTGAAAAGGTAGCTGTGCTACCTTTAATAGTAG
CTATGTTTATAATAGGTGTATATCCTAAATTTGTATTAAACATTTTATTGATACCAGCTAAGAACTTAAGCAGTTTATTT
ATTATATAA

Upstream 100 bases:

>100_bases
GACTGCATTTTTTACTTTAATTGTTATTAATTACTTTATAACTAGTAACTAAATGCCAATAGTAACTCAAGATATTCCTA
TATTATCAGTTTGTATTTCT

Downstream 100 bases:

>100_bases
ATTGTAATTGCTGTGTACGTTTAATTTTATTTGAAAGAGTTATTATGGAAATGTTATATGGTATTATGCCAGAAATTTCG
CTTCTTTTATCAGCTTTGAT

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MPLSALIILLLMRLSNYFNKLVYVKFIALLSSLLTLLCTIYLLLQFDNTAHTYQFVELYSIFKLIGLNYHVGIDGISIFF
IALTALLTLLSIVISIFTVQKKLEDNLLCFLLIESLVIGAFSSMNLLLFFMFFEASLFPIFLLIGIWGSSNRIYAALKFF
LYSFVGSVFFLVAIIYIHTYTGTLEITELYDLMPRFDISVQMLLWLSIFVAFAIKIPMLPLHSWLPDAHVEAQTGGSVML
AGILLKLGGFGLLRICLPMLPAASQKFSSIAMIFSVAAIIYFSIIAFQQIDIKKAIAYSSIAHMGYVTVGIFSLNTYGIQ
GAIFQMLSHGLISPALFMIIGILYDRTHTREIRFYGGLAVKMTKLASIFMAAVLGSIGVPGTSGFIGEFLVLLGAITASP
LIGSFAILGVLLGAVYMLSLYRRIMLGEITNQRVYQLNDILLSEKVAVLPLIVAMFIIGVYPKFVLNILLIPAKNLSSLF
II

Sequences:

>Translated_482_residues
MPLSALIILLLMRLSNYFNKLVYVKFIALLSSLLTLLCTIYLLLQFDNTAHTYQFVELYSIFKLIGLNYHVGIDGISIFF
IALTALLTLLSIVISIFTVQKKLEDNLLCFLLIESLVIGAFSSMNLLLFFMFFEASLFPIFLLIGIWGSSNRIYAALKFF
LYSFVGSVFFLVAIIYIHTYTGTLEITELYDLMPRFDISVQMLLWLSIFVAFAIKIPMLPLHSWLPDAHVEAQTGGSVML
AGILLKLGGFGLLRICLPMLPAASQKFSSIAMIFSVAAIIYFSIIAFQQIDIKKAIAYSSIAHMGYVTVGIFSLNTYGIQ
GAIFQMLSHGLISPALFMIIGILYDRTHTREIRFYGGLAVKMTKLASIFMAAVLGSIGVPGTSGFIGEFLVLLGAITASP
LIGSFAILGVLLGAVYMLSLYRRIMLGEITNQRVYQLNDILLSEKVAVLPLIVAMFIIGVYPKFVLNILLIPAKNLSSLF
II
>Mature_481_residues
PLSALIILLLMRLSNYFNKLVYVKFIALLSSLLTLLCTIYLLLQFDNTAHTYQFVELYSIFKLIGLNYHVGIDGISIFFI
ALTALLTLLSIVISIFTVQKKLEDNLLCFLLIESLVIGAFSSMNLLLFFMFFEASLFPIFLLIGIWGSSNRIYAALKFFL
YSFVGSVFFLVAIIYIHTYTGTLEITELYDLMPRFDISVQMLLWLSIFVAFAIKIPMLPLHSWLPDAHVEAQTGGSVMLA
GILLKLGGFGLLRICLPMLPAASQKFSSIAMIFSVAAIIYFSIIAFQQIDIKKAIAYSSIAHMGYVTVGIFSLNTYGIQG
AIFQMLSHGLISPALFMIIGILYDRTHTREIRFYGGLAVKMTKLASIFMAAVLGSIGVPGTSGFIGEFLVLLGAITASPL
IGSFAILGVLLGAVYMLSLYRRIMLGEITNQRVYQLNDILLSEKVAVLPLIVAMFIIGVYPKFVLNILLIPAKNLSSLFI
I

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=335, Percent_Identity=34.6268656716418, Blast_Score=177, Evalue=3e-44,
Organism=Escherichia coli, GI1788613, Length=474, Percent_Identity=37.1308016877637, Blast_Score=285, Evalue=6e-78,
Organism=Escherichia coli, GI1788827, Length=281, Percent_Identity=28.4697508896797, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788831, Length=349, Percent_Identity=26.3610315186246, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693160, Length=343, Percent_Identity=24.7813411078717, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788829, Length=377, Percent_Identity=26.790450928382, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788614, Length=293, Percent_Identity=26.2798634812287, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53435; Mature: 53303

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSALIILLLMRLSNYFNKLVYVKFIALLSSLLTLLCTIYLLLQFDNTAHTYQFVELYS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IFKLIGLNYHVGIDGISIFFIALTALLTLLSIVISIFTVQKKLEDNLLCFLLIESLVIGA
HHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FSSMNLLLFFMFFEASLFPIFLLIGIWGSSNRIYAALKFFLYSFVGSVFFLVAIIYIHTY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TGTLEITELYDLMPRFDISVQMLLWLSIFVAFAIKIPMLPLHSWLPDAHVEAQTGGSVML
CCCEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHHH
AGILLKLGGFGLLRICLPMLPAASQKFSSIAMIFSVAAIIYFSIIAFQQIDIKKAIAYSS
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAHMGYVTVGIFSLNTYGIQGAIFQMLSHGLISPALFMIIGILYDRTHTREIRFYGGLAV
HHHHHHHHHHEEECCHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCHHH
KMTKLASIFMAAVLGSIGVPGTSGFIGEFLVLLGAITASPLIGSFAILGVLLGAVYMLSL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRRIMLGEITNQRVYQLNDILLSEKVAVLPLIVAMFIIGVYPKFVLNILLIPAKNLSSLF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
II
CC
>Mature Secondary Structure 
PLSALIILLLMRLSNYFNKLVYVKFIALLSSLLTLLCTIYLLLQFDNTAHTYQFVELYS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH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CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA