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The map label for this gene is folD

Identifier: 148285002

GI number: 148285002

Start: 1776232

End: 1777137

Strand: Direct

Name: folD

Synonym: OTBS_1842

Alternate gene names: 148285002

Gene position: 1776232-1777137 (Clockwise)

Preceding gene: 148285001

Following gene: 148285007

Centisome position: 83.51

GC content: 31.13

Gene sequence:

>906_bases
ATGACAGTAATTATGGATGGTAAAAAGCTTGCTGAGTTGAGGTTGATAGAAACAAAAAATGACTTACTAGCTCTTAAAGA
TAAATATCAAATAACAGTTAAGTTGGTAATAATTTTAGTTGGTAACAATGATGCAAGCTTGATTTATGTTAATAATAAGG
TAGCTAAAGCTAAGGCTATTGGTATGGATTCAGAAATAATTAGGTTATTCGAATTCATAGAGGAAAAAAAGTTATTTTCT
GTTATTGATGACTTAAATTGTGATAGTACAGTACATGGTATTATTGTTCAATTGCCTCTTCCGCCTCATATCGACGCTTT
AAAATTATTTGCAAGAATTGATTCTAGAAAAGATGTTGATGGGCTTAACCCGATAAATATAGGGTATCTTAATATTGGAG
CTAACCAAGGATTAATTCCTTGTACAGCTTTAGGATGCATAGATTTATTACAGTATTATGTCACAGGTTTAAAGGGGAAA
CATGTAGTTGTAATAGGAAAATCCAATATTGTTGGTAAACCTCTATCAGCATTGCTGTTACGTAATTCTTGTACTGTTAC
TATATGTCATTCTGCTACTGTTGATCTAGCCTTGCATACTAGAACAGCTGATATTGTAATTTCAGCTGTTGGAAAAGCGA
ATTTTTTAACAGATAAGCATTTTTCAGGTAATTTAGCTTTTATTGATGTTGGAATAAGTCATATTTATGATTTGCAAACT
CATAAACGAAAATTAGTTGGAGATGGAGATTTTTTAAAAATCAAAGATTTAGTAAAATTTATTACTCCAGTTCCAGGCGG
AGTAGGGCCAATGACAGTAGCATATCTATTAAAAAATACATTAACTGCTGCTAAACTTATTTATGCGAGCATTATAGATA
ATGACAATGAAAAACGCTTGTGTTAG

Upstream 100 bases:

>100_bases
GCTTTGCTGAAGCTGCAACTGCAATGAGTCATTGTTATCAAAGAGTTTTTGGAAAAAAAATGCATTTTCAATACTCTACT
GTAAAGGGAGTATTACGTTC

Downstream 100 bases:

>100_bases
AATAGATATTATTTTACTATAACATGCTACATTAGTAATTATCTTTACTAAATGTAAGGTCGAATTAATATGATCAAAAT
CTATTAGATTTAATAAACAA

Product: methylenetetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTVIMDGKKLAELRLIETKNDLLALKDKYQITVKLVIILVGNNDASLIYVNNKVAKAKAIGMDSEIIRLFEFIEEKKLFS
VIDDLNCDSTVHGIIVQLPLPPHIDALKLFARIDSRKDVDGLNPINIGYLNIGANQGLIPCTALGCIDLLQYYVTGLKGK
HVVVIGKSNIVGKPLSALLLRNSCTVTICHSATVDLALHTRTADIVISAVGKANFLTDKHFSGNLAFIDVGISHIYDLQT
HKRKLVGDGDFLKIKDLVKFITPVPGGVGPMTVAYLLKNTLTAAKLIYASIIDNDNEKRLC

Sequences:

>Translated_301_residues
MTVIMDGKKLAELRLIETKNDLLALKDKYQITVKLVIILVGNNDASLIYVNNKVAKAKAIGMDSEIIRLFEFIEEKKLFS
VIDDLNCDSTVHGIIVQLPLPPHIDALKLFARIDSRKDVDGLNPINIGYLNIGANQGLIPCTALGCIDLLQYYVTGLKGK
HVVVIGKSNIVGKPLSALLLRNSCTVTICHSATVDLALHTRTADIVISAVGKANFLTDKHFSGNLAFIDVGISHIYDLQT
HKRKLVGDGDFLKIKDLVKFITPVPGGVGPMTVAYLLKNTLTAAKLIYASIIDNDNEKRLC
>Mature_300_residues
TVIMDGKKLAELRLIETKNDLLALKDKYQITVKLVIILVGNNDASLIYVNNKVAKAKAIGMDSEIIRLFEFIEEKKLFSV
IDDLNCDSTVHGIIVQLPLPPHIDALKLFARIDSRKDVDGLNPINIGYLNIGANQGLIPCTALGCIDLLQYYVTGLKGKH
VVVIGKSNIVGKPLSALLLRNSCTVTICHSATVDLALHTRTADIVISAVGKANFLTDKHFSGNLAFIDVGISHIYDLQTH
KRKLVGDGDFLKIKDLVKFITPVPGGVGPMTVAYLLKNTLTAAKLIYASIIDNDNEKRLC

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=287, Percent_Identity=40.0696864111498, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI222418558, Length=265, Percent_Identity=46.7924528301887, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=40.5405405405405, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI1786741, Length=280, Percent_Identity=42.5, Blast_Score=194, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17568735, Length=270, Percent_Identity=44.4444444444444, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=41.0526315789474, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321643, Length=293, Percent_Identity=39.9317406143345, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322933, Length=198, Percent_Identity=28.7878787878788, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=40.4040404040404, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=40.4040404040404, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI62472483, Length=285, Percent_Identity=38.9473684210526, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI45551871, Length=285, Percent_Identity=38.9473684210526, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24645718, Length=285, Percent_Identity=38.9473684210526, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI17137370, Length=285, Percent_Identity=38.9473684210526, Blast_Score=185, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_ORITB (A5CF58)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001249092.1
- ProteinModelPortal:   A5CF58
- SMR:   A5CF58
- STRING:   A5CF58
- GeneID:   5218224
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1842
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- ProtClustDB:   CLSK2325885
- BioCyc:   OTSU357244:OTBS_1842-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32885; Mature: 32754

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIMDGKKLAELRLIETKNDLLALKDKYQITVKLVIILVGNNDASLIYVNNKVAKAKAI
CEEEECCCCEEEEEEEECCCCEEEECCCEEEEEEEEEEEECCCCEEEEEEECCEEEHHHC
GMDSEIIRLFEFIEEKKLFSVIDDLNCDSTVHGIIVQLPLPPHIDALKLFARIDSRKDVD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCC
GLNPINIGYLNIGANQGLIPCTALGCIDLLQYYVTGLKGKHVVVIGKSNIVGKPLSALLL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHH
RNSCTVTICHSATVDLALHTRTADIVISAVGKANFLTDKHFSGNLAFIDVGISHIYDLQT
CCCCEEEEEECCEEEEEEEECCCEEEEEECCCCCCEECCCCCCCEEEEEECHHHHHHHHH
HKRKLVGDGDFLKIKDLVKFITPVPGGVGPMTVAYLLKNTLTAAKLIYASIIDNDNEKRL
HHHHHCCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
C
C
>Mature Secondary Structure 
TVIMDGKKLAELRLIETKNDLLALKDKYQITVKLVIILVGNNDASLIYVNNKVAKAKAI
EEEECCCCEEEEEEEECCCCEEEECCCEEEEEEEEEEEECCCCEEEEEEECCEEEHHHC
GMDSEIIRLFEFIEEKKLFSVIDDLNCDSTVHGIIVQLPLPPHIDALKLFARIDSRKDVD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCC
GLNPINIGYLNIGANQGLIPCTALGCIDLLQYYVTGLKGKHVVVIGKSNIVGKPLSALLL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHH
RNSCTVTICHSATVDLALHTRTADIVISAVGKANFLTDKHFSGNLAFIDVGISHIYDLQT
CCCCEEEEEECCEEEEEEEECCCEEEEEECCCCCCEECCCCCCCEEEEEECHHHHHHHHH
HKRKLVGDGDFLKIKDLVKFITPVPGGVGPMTVAYLLKNTLTAAKLIYASIIDNDNEKRL
HHHHHCCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
C
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA