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The map label for this gene is xth1 [H]

Identifier: 148284985

GI number: 148284985

Start: 1746339

End: 1747160

Strand: Direct

Name: xth1 [H]

Synonym: OTBS_1807

Alternate gene names: 148284985

Gene position: 1746339-1747160 (Clockwise)

Preceding gene: 148284983

Following gene: 148284986

Centisome position: 82.1

GC content: 29.44

Gene sequence:

>822_bases
TTGGTTACAAATATACTAAATATTATTATGAGCCGTAAACTAAAAATTTTAACTTGGAATATTAATTCTATTAGGGCAAG
AGCATATCAACTAAACCAACTTATTTATGATATACAGCCAGATATTATTTTATTACAGGAAATCAAATGCGAAAGCCATA
ACTTTCCTTTTCAAGAAATTAATGAGTCACATAAATATAATTTCAGTATTAATGGCCAGAAGTCATACAATGGTGTTGCA
ATACTATCAAAATTCCCTCTAGAAGATATAAAATTTACTTTTTTGGGCAACCCTTGCCCAGATCAAGCAAGATTCATTGA
AGCAAATTGTTTAACTGAAGTAGGATATGTTAAAGTTATTTCAATTTATGTTCCAAATGGAGGAGAAGTCAATAGTGATA
AATTTCAAATTAAGCTACTTTTCATTGATAAATTTATTAAGTACTTACATAGTAGTAAAAATTTAGGTGAATCAATAATA
TTGGGTGGAGATTTTAATGTTGCACCATACGATAATGATGTTTATTCTGCTATAGAGTTACAAAATACAACTTGTTTTAC
CTTGCCTGAAAAGCAATATATACGTAAGTTAATAAATCATAATTTTATAGATATTTATCGATTATTTCACCAGCGACAAA
AAAAATTTACGTGGTGGGATTATCGAGCTGGAGCATTTGAGCGTAACTTAGGAATGCGTATAGATTATATACTTTGTACG
ATCTCTATTGCTCTAAAAGCTACAGATTGCTATATTGATTATCATACTAGAAACAATCATAGAGCTTCAGATCATGCTCC
TGTCATTGCTAGTTTTGAGTGA

Upstream 100 bases:

>100_bases
ATTGATAAAAAGTATAAATTGCAAACGAGATAATAACCCTTTATGCAGAAATACATTATTTGTTTTACAGATGATACTAA
CTTACATCTTGTAAAAAGTT

Downstream 100 bases:

>100_bases
TTTCAAATTCTTGTTAGTTCTATGAAAGTTGATATTGAATCAATTATATTATTACTGCAGTTAAAAATAATAGATCTCAA
TTAAGCATGCTCAATACTTA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MVTNILNIIMSRKLKILTWNINSIRARAYQLNQLIYDIQPDIILLQEIKCESHNFPFQEINESHKYNFSINGQKSYNGVA
ILSKFPLEDIKFTFLGNPCPDQARFIEANCLTEVGYVKVISIYVPNGGEVNSDKFQIKLLFIDKFIKYLHSSKNLGESII
LGGDFNVAPYDNDVYSAIELQNTTCFTLPEKQYIRKLINHNFIDIYRLFHQRQKKFTWWDYRAGAFERNLGMRIDYILCT
ISIALKATDCYIDYHTRNNHRASDHAPVIASFE

Sequences:

>Translated_273_residues
MVTNILNIIMSRKLKILTWNINSIRARAYQLNQLIYDIQPDIILLQEIKCESHNFPFQEINESHKYNFSINGQKSYNGVA
ILSKFPLEDIKFTFLGNPCPDQARFIEANCLTEVGYVKVISIYVPNGGEVNSDKFQIKLLFIDKFIKYLHSSKNLGESII
LGGDFNVAPYDNDVYSAIELQNTTCFTLPEKQYIRKLINHNFIDIYRLFHQRQKKFTWWDYRAGAFERNLGMRIDYILCT
ISIALKATDCYIDYHTRNNHRASDHAPVIASFE
>Mature_273_residues
MVTNILNIIMSRKLKILTWNINSIRARAYQLNQLIYDIQPDIILLQEIKCESHNFPFQEINESHKYNFSINGQKSYNGVA
ILSKFPLEDIKFTFLGNPCPDQARFIEANCLTEVGYVKVISIYVPNGGEVNSDKFQIKLLFIDKFIKYLHSSKNLGESII
LGGDFNVAPYDNDVYSAIELQNTTCFTLPEKQYIRKLINHNFIDIYRLFHQRQKKFTWWDYRAGAFERNLGMRIDYILCT
ISIALKATDCYIDYHTRNNHRASDHAPVIASFE

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=28.7878787878788, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=28.7878787878788, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=28.7878787878788, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=33.7078651685393, Blast_Score=151, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71989536, Length=267, Percent_Identity=27.7153558052434, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI221330655, Length=265, Percent_Identity=30.188679245283, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17136678, Length=265, Percent_Identity=30.188679245283, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31828; Mature: 31828

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTNILNIIMSRKLKILTWNINSIRARAYQLNQLIYDIQPDIILLQEIKCESHNFPFQEI
CHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHC
NESHKYNFSINGQKSYNGVAILSKFPLEDIKFTFLGNPCPDQARFIEANCLTEVGYVKVI
CCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHEEEEHHHHCCCCEEEEE
SIYVPNGGEVNSDKFQIKLLFIDKFIKYLHSSKNLGESIILGGDFNVAPYDNDVYSAIEL
EEEECCCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCEEEECCCCEECCCCCCCEEEEEE
QNTTCFTLPEKQYIRKLINHNFIDIYRLFHQRQKKFTWWDYRAGAFERNLGMRIDYILCT
CCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEEE
ISIALKATDCYIDYHTRNNHRASDHAPVIASFE
EEEEEEEEEEEEEEECCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MVTNILNIIMSRKLKILTWNINSIRARAYQLNQLIYDIQPDIILLQEIKCESHNFPFQEI
CHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHC
NESHKYNFSINGQKSYNGVAILSKFPLEDIKFTFLGNPCPDQARFIEANCLTEVGYVKVI
CCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHEEEEHHHHCCCCEEEEE
SIYVPNGGEVNSDKFQIKLLFIDKFIKYLHSSKNLGESIILGGDFNVAPYDNDVYSAIEL
EEEECCCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCEEEECCCCEECCCCCCCEEEEEE
QNTTCFTLPEKQYIRKLINHNFIDIYRLFHQRQKKFTWWDYRAGAFERNLGMRIDYILCT
CCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEEE
ISIALKATDCYIDYHTRNNHRASDHAPVIASFE
EEEEEEEEEEEEEEECCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]