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The map label for this gene is hisS

Identifier: 148284858

GI number: 148284858

Start: 1545460

End: 1546731

Strand: Direct

Name: hisS

Synonym: OTBS_1585

Alternate gene names: 148284858

Gene position: 1545460-1546731 (Clockwise)

Preceding gene: 148284857

Following gene: 148284859

Centisome position: 72.66

GC content: 30.9

Gene sequence:

>1272_bases
ATGTCAATCAAATTACAGCCAGTTAAAGGTAGCAAAGATTTACTACCTGAAGAATTTGGAAAACATGACTACATTATTAG
CGTTTCTAGGAATTTAAGTAAGTTATATGGATTTCAACCTATATCTACTCCAATCATAGAATACACAGAAATATTTAACC
GTACTTTAGGTAAAGACTCAGATGTTCTTAGTAAAGAGATGTACGTTTTTCTTGATAAAGGAAATCGTAGTGTTAGTTTA
AGGCCAGAATTTACTGCGAGTATTATGCGTGCAGTTATCTATAATAATCTACAAAATAAAAAACTACCATTAAAATATTT
TTCATCAGGACCAGCATTTAGGTATGATAATCCTCAAGCTGGTAGACAGCGTCAATTTCATCAAATCAATTTGGAGTGTA
TTGGTGATGGCTCTCCATTTAGTGATGCAGAAATAGTTCTGCTAGCTTATGATATATTAAAAAAATTAAATTTAGTTGAT
AAAGTAAACTTAGAAATAAATTCACTTGGCTGCATGGAATCAAGAGCAAAATATCAACAGGCTTTGGTTGAGTATTTTAG
TAAATACAGAACTGAATTATCACAAGATAGTCAAAGTCGTCTTATTGAAAATCCACTGCGAATTTTAGATTCTAAAAATT
TGCATGATAAAAAAATATCTGCATCAGCACCTAGTATACATGATTATTACACTATTGAAGCTAGAGGTTACTTTGATAAA
GTGCTAGAATATTTAGAATTTTGTGGTATTAAATATACTATAAATGCTAATTTAGTACGTGGGTTAGATTATTACTGTCA
TACAGTTTTTGAGTATGTATCAACTCAAATTGGAGCTCAATCTACTGTTTTGGGAGGAGGGAGATATGATGGACTATTCG
AACAAATGGGAGGTAAATTAGCAAGTGGCAAAAAAATGTTACCTGCTATTGGTTTTGCTGCTGGAATAGAAAGATTAGCA
CTTTTAACTAATTATACCCCAGTGGATGTAAGACCTATAGTTATTATACCAGTAGATGAAGAATATCACCAACACGGTAT
TATATTGCTGCAAAAATTAAGAAATAATAATATTACAACTGTAATTGATTTGCAGGACAGCATTTCAAAAAGATTAAATC
GAGCTAATCACATTAAGGCAAGTAAGGTAATATTTATTGGAGCAATCGAAATGCGAGAACAAAGTTATAGAATCAAAGAC
TTAGATACTAGTAATGAATGTGTAATAATACATAATGAACTACTTAGCTATTTGCAAAATAACTGCTCTTAA

Upstream 100 bases:

>100_bases
TTACACAATCAGCTATAAATGTTGAAAGAATGATTGGCAAAGTATTCTCTGAACTTTTTGGCAGTAATTATTTTAAAATC
TCTTCTAGCAGGCATTAAAA

Downstream 100 bases:

>100_bases
TATCAAATTGGTTGTTCTATTATATGATTAAGATTAATACTAAGACTATAACTAGAATTGCGAGCATTCAAGGAGTTTAT
AGTTACCAAATTTTAGACTC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSIKLQPVKGSKDLLPEEFGKHDYIISVSRNLSKLYGFQPISTPIIEYTEIFNRTLGKDSDVLSKEMYVFLDKGNRSVSL
RPEFTASIMRAVIYNNLQNKKLPLKYFSSGPAFRYDNPQAGRQRQFHQINLECIGDGSPFSDAEIVLLAYDILKKLNLVD
KVNLEINSLGCMESRAKYQQALVEYFSKYRTELSQDSQSRLIENPLRILDSKNLHDKKISASAPSIHDYYTIEARGYFDK
VLEYLEFCGIKYTINANLVRGLDYYCHTVFEYVSTQIGAQSTVLGGGRYDGLFEQMGGKLASGKKMLPAIGFAAGIERLA
LLTNYTPVDVRPIVIIPVDEEYHQHGIILLQKLRNNNITTVIDLQDSISKRLNRANHIKASKVIFIGAIEMREQSYRIKD
LDTSNECVIIHNELLSYLQNNCS

Sequences:

>Translated_423_residues
MSIKLQPVKGSKDLLPEEFGKHDYIISVSRNLSKLYGFQPISTPIIEYTEIFNRTLGKDSDVLSKEMYVFLDKGNRSVSL
RPEFTASIMRAVIYNNLQNKKLPLKYFSSGPAFRYDNPQAGRQRQFHQINLECIGDGSPFSDAEIVLLAYDILKKLNLVD
KVNLEINSLGCMESRAKYQQALVEYFSKYRTELSQDSQSRLIENPLRILDSKNLHDKKISASAPSIHDYYTIEARGYFDK
VLEYLEFCGIKYTINANLVRGLDYYCHTVFEYVSTQIGAQSTVLGGGRYDGLFEQMGGKLASGKKMLPAIGFAAGIERLA
LLTNYTPVDVRPIVIIPVDEEYHQHGIILLQKLRNNNITTVIDLQDSISKRLNRANHIKASKVIFIGAIEMREQSYRIKD
LDTSNECVIIHNELLSYLQNNCS
>Mature_422_residues
SIKLQPVKGSKDLLPEEFGKHDYIISVSRNLSKLYGFQPISTPIIEYTEIFNRTLGKDSDVLSKEMYVFLDKGNRSVSLR
PEFTASIMRAVIYNNLQNKKLPLKYFSSGPAFRYDNPQAGRQRQFHQINLECIGDGSPFSDAEIVLLAYDILKKLNLVDK
VNLEINSLGCMESRAKYQQALVEYFSKYRTELSQDSQSRLIENPLRILDSKNLHDKKISASAPSIHDYYTIEARGYFDKV
LEYLEFCGIKYTINANLVRGLDYYCHTVFEYVSTQIGAQSTVLGGGRYDGLFEQMGGKLASGKKMLPAIGFAAGIERLAL
LTNYTPVDVRPIVIIPVDEEYHQHGIILLQKLRNNNITTVIDLQDSISKRLNRANHIKASKVIFIGAIEMREQSYRIKDL
DTSNECVIIHNELLSYLQNNCS

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=352, Percent_Identity=28.125, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1788861, Length=430, Percent_Identity=36.2790697674419, Blast_Score=286, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6325290, Length=374, Percent_Identity=25.1336898395722, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24643061, Length=363, Percent_Identity=23.9669421487603, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24643059, Length=382, Percent_Identity=22.7748691099476, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_ORITB (A5CEP8)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248948.1
- ProteinModelPortal:   A5CEP8
- SMR:   A5CEP8
- STRING:   A5CEP8
- GeneID:   5217964
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1585
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   OTSU357244:OTBS_1585-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48116; Mature: 47985

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLQPVKGSKDLLPEEFGKHDYIISVSRNLSKLYGFQPISTPIIEYTEIFNRTLGKDS
CCEEEECCCCCCCCCHHHHCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
DVLSKEMYVFLDKGNRSVSLRPEFTASIMRAVIYNNLQNKKLPLKYFSSGPAFRYDNPQA
HHHCCEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCC
GRQRQFHQINLECIGDGSPFSDAEIVLLAYDILKKLNLVDKVNLEINSLGCMESRAKYQQ
CCCCHHEEEEEEEEECCCCCCCCEEEEHHHHHHHHHCCHHCCCEEEECCCCHHHHHHHHH
ALVEYFSKYRTELSQDSQSRLIENPLRILDSKNLHDKKISASAPSIHDYYTIEARGYFDK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
VLEYLEFCGIKYTINANLVRGLDYYCHTVFEYVSTQIGAQSTVLGGGRYDGLFEQMGGKL
HHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHCCCEE
ASGKKMLPAIGFAAGIERLALLTNYTPVDVRPIVIIPVDEEYHQHGIILLQKLRNNNITT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHCHHHEEEECCCCCCEE
VIDLQDSISKRLNRANHIKASKVIFIGAIEMREQSYRIKDLDTSNECVIIHNELLSYLQN
EEECHHHHHHHHHHHCCCCCCEEEEEEEEEHHCCCCEEEECCCCCCEEEEHHHHHHHHHH
NCS
CCC
>Mature Secondary Structure 
SIKLQPVKGSKDLLPEEFGKHDYIISVSRNLSKLYGFQPISTPIIEYTEIFNRTLGKDS
CEEEECCCCCCCCCHHHHCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
DVLSKEMYVFLDKGNRSVSLRPEFTASIMRAVIYNNLQNKKLPLKYFSSGPAFRYDNPQA
HHHCCEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCC
GRQRQFHQINLECIGDGSPFSDAEIVLLAYDILKKLNLVDKVNLEINSLGCMESRAKYQQ
CCCCHHEEEEEEEEECCCCCCCCEEEEHHHHHHHHHCCHHCCCEEEECCCCHHHHHHHHH
ALVEYFSKYRTELSQDSQSRLIENPLRILDSKNLHDKKISASAPSIHDYYTIEARGYFDK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
VLEYLEFCGIKYTINANLVRGLDYYCHTVFEYVSTQIGAQSTVLGGGRYDGLFEQMGGKL
HHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHCCCEE
ASGKKMLPAIGFAAGIERLALLTNYTPVDVRPIVIIPVDEEYHQHGIILLQKLRNNNITT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHCHHHEEEECCCCCCEE
VIDLQDSISKRLNRANHIKASKVIFIGAIEMREQSYRIKDLDTSNECVIIHNELLSYLQN
EEECHHHHHHHHHHHCCCCCCEEEEEEEEEHHCCCCEEEECCCCCCEEEEHHHHHHHHHH
NCS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA