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The map label for this gene is proP [H]

Identifier: 148284805

GI number: 148284805

Start: 1455919

End: 1457220

Strand: Direct

Name: proP [H]

Synonym: OTBS_1493

Alternate gene names: 148284805

Gene position: 1455919-1457220 (Clockwise)

Preceding gene: 148284804

Following gene: 148284806

Centisome position: 68.45

GC content: 32.18

Gene sequence:

>1302_bases
ATGTATAAAAATAAGCATAGGTTTATCTTTCTTGCAGCAATATTTGGAAATATATTAGAGTATTTTGACTACACGCTATA
TACAATTTTTGTATTACAATTGGGACAAGCCTTTTTTCCAAAAAGTTCTGAATTTGTACAAGTAATCTCAGCCTTATCTC
TATTTGCTGTAGGCTTTCTTGCTAGGCCTATAGGCGGAATATTTTTTGGTTATATTGGTGATAAGTATGGTAGAAAAACA
TCTTTAGTTATATCAATGTTAGGCATGACAATTCCTACTTTTTTAATAGGATTAATTCCTACTTTTAGCCAAATTGGCTA
TCTAGCACCACTTGCACTAGCATTATTGCGGTTAATGCAAGGGCTATGCTTAAGCGGTGAGGGAGCAGGAACAGCTATTT
TTGTTCTAGAACACCAACATAATTTAAAACCAGGATTAGTTACAGGGTTGGTACATGGGTCCAATACTGCAGGGATGCTA
CTAGCTGCTCTGGTAGGAATAATAGTTGAAAATTATTATTCTGATATTACATATGCTTGGAGAATAGCATTTTTACTAGG
TGGTATTTTGGGGTTAATAGGACTCTATATACGGGTGAAAGTTGCTGAAACTCCAGTGTTTTTAGAATTAGTCAAAAAGA
GGAATATATTAAAATATCCACTAATTCATGTTATTAAAACTGCTAAACATAAAATGTTGATAACTTTCTGTGTTGGCGGA
GTAACAAGTAGTATTGTATATCTTGTTAAGAGTGTTAATGTTTTCTATAAAACTATATTACATTTTAATAATAGCAGCGC
TTTATATTATTTGTTTTATACTTCTTGTATTTTAATGATTTGCATGCCAATATCTGGTGCTATTTCTGATTTTTATGGTC
GAACTAAAGTAATGATATGCTCAGTTGCTGCTACAGTAATTGCGGCAGTTGCAGTTTTTTTTCTTATGTCATCAGATATT
ATGTGGCATCAACTTTTAGGATTGACAGGTCTTGGTATACTTGCAGGAGGAGTCTCAGGAGTATCTTATATATTTATAAT
TTCATTATTTACTCCTGAACAACGATTTACTGGTATAGCTTTTAGTTTTAACTTAGGTAATGCTATATTTGGCGGTACTG
CTCCAACAATTACAACTTGGTTAGCTGGAACAATAAAACATAATTATGGTCCAACACTTGGACATATTGCTCCAGCTTTT
TATATTATGACTACTTCAACAATCTTTTTGTTGATAATGTATTTTACATATCACTGGATCAAGGAGTATGATAATCAACA
AAATTTCAGTAATAAGCATTAA

Upstream 100 bases:

>100_bases
TATCAATGCATCATTTATTTTACAGAAATATTGACGATTACTTTCTATCATAATCATCGTAGATCAACAAATAATAGTTT
AACAATTTGGTTAAAGTTTT

Downstream 100 bases:

>100_bases
AATTTGCACTTAACAATGTGCTTTCTTATATACAAGGCCTAATAATTTAAAAAGCTAAGAGTATTGATGATAAGTAGTTA
CAATTTAGGAGTATTAGCTG

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MYKNKHRFIFLAAIFGNILEYFDYTLYTIFVLQLGQAFFPKSSEFVQVISALSLFAVGFLARPIGGIFFGYIGDKYGRKT
SLVISMLGMTIPTFLIGLIPTFSQIGYLAPLALALLRLMQGLCLSGEGAGTAIFVLEHQHNLKPGLVTGLVHGSNTAGML
LAALVGIIVENYYSDITYAWRIAFLLGGILGLIGLYIRVKVAETPVFLELVKKRNILKYPLIHVIKTAKHKMLITFCVGG
VTSSIVYLVKSVNVFYKTILHFNNSSALYYLFYTSCILMICMPISGAISDFYGRTKVMICSVAATVIAAVAVFFLMSSDI
MWHQLLGLTGLGILAGGVSGVSYIFIISLFTPEQRFTGIAFSFNLGNAIFGGTAPTITTWLAGTIKHNYGPTLGHIAPAF
YIMTTSTIFLLIMYFTYHWIKEYDNQQNFSNKH

Sequences:

>Translated_433_residues
MYKNKHRFIFLAAIFGNILEYFDYTLYTIFVLQLGQAFFPKSSEFVQVISALSLFAVGFLARPIGGIFFGYIGDKYGRKT
SLVISMLGMTIPTFLIGLIPTFSQIGYLAPLALALLRLMQGLCLSGEGAGTAIFVLEHQHNLKPGLVTGLVHGSNTAGML
LAALVGIIVENYYSDITYAWRIAFLLGGILGLIGLYIRVKVAETPVFLELVKKRNILKYPLIHVIKTAKHKMLITFCVGG
VTSSIVYLVKSVNVFYKTILHFNNSSALYYLFYTSCILMICMPISGAISDFYGRTKVMICSVAATVIAAVAVFFLMSSDI
MWHQLLGLTGLGILAGGVSGVSYIFIISLFTPEQRFTGIAFSFNLGNAIFGGTAPTITTWLAGTIKHNYGPTLGHIAPAF
YIMTTSTIFLLIMYFTYHWIKEYDNQQNFSNKH
>Mature_433_residues
MYKNKHRFIFLAAIFGNILEYFDYTLYTIFVLQLGQAFFPKSSEFVQVISALSLFAVGFLARPIGGIFFGYIGDKYGRKT
SLVISMLGMTIPTFLIGLIPTFSQIGYLAPLALALLRLMQGLCLSGEGAGTAIFVLEHQHNLKPGLVTGLVHGSNTAGML
LAALVGIIVENYYSDITYAWRIAFLLGGILGLIGLYIRVKVAETPVFLELVKKRNILKYPLIHVIKTAKHKMLITFCVGG
VTSSIVYLVKSVNVFYKTILHFNNSSALYYLFYTSCILMICMPISGAISDFYGRTKVMICSVAATVIAAVAVFFLMSSDI
MWHQLLGLTGLGILAGGVSGVSYIFIISLFTPEQRFTGIAFSFNLGNAIFGGTAPTITTWLAGTIKHNYGPTLGHIAPAF
YIMTTSTIFLLIMYFTYHWIKEYDNQQNFSNKH

Specific function: Involved in uptake and accumulation of various osmoprotectants. Allows the uptake of glycine betaine, proline, ectoine, and pipecolic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=423, Percent_Identity=30.2600472813239, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1789941, Length=408, Percent_Identity=30.6372549019608, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1788292, Length=392, Percent_Identity=26.7857142857143, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788942, Length=387, Percent_Identity=28.9405684754522, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87082231, Length=430, Percent_Identity=22.5581395348837, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082404, Length=393, Percent_Identity=25.4452926208651, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47803; Mature: 47803

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKNKHRFIFLAAIFGNILEYFDYTLYTIFVLQLGQAFFPKSSEFVQVISALSLFAVGFL
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ARPIGGIFFGYIGDKYGRKTSLVISMLGMTIPTFLIGLIPTFSQIGYLAPLALALLRLMQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLCLSGEGAGTAIFVLEHQHNLKPGLVTGLVHGSNTAGMLLAALVGIIVENYYSDITYAW
CCEECCCCCCEEEEEEECCCCCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
RIAFLLGGILGLIGLYIRVKVAETPVFLELVKKRNILKYPLIHVIKTAKHKMLITFCVGG
HHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHEEHHHHHHH
VTSSIVYLVKSVNVFYKTILHFNNSSALYYLFYTSCILMICMPISGAISDFYGRTKVMIC
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHH
SVAATVIAAVAVFFLMSSDIMWHQLLGLTGLGILAGGVSGVSYIFIISLFTPEQRFTGIA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHEEHHEEEEECCCHHHCEEE
FSFNLGNAIFGGTAPTITTWLAGTIKHNYGPTLGHIAPAFYIMTTSTIFLLIMYFTYHWI
EEEECCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEYDNQQNFSNKH
HHHCCCCCCCCCC
>Mature Secondary Structure
MYKNKHRFIFLAAIFGNILEYFDYTLYTIFVLQLGQAFFPKSSEFVQVISALSLFAVGFL
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ARPIGGIFFGYIGDKYGRKTSLVISMLGMTIPTFLIGLIPTFSQIGYLAPLALALLRLMQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLCLSGEGAGTAIFVLEHQHNLKPGLVTGLVHGSNTAGMLLAALVGIIVENYYSDITYAW
CCEECCCCCCEEEEEEECCCCCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
RIAFLLGGILGLIGLYIRVKVAETPVFLELVKKRNILKYPLIHVIKTAKHKMLITFCVGG
HHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHEEHHHHHHH
VTSSIVYLVKSVNVFYKTILHFNNSSALYYLFYTSCILMICMPISGAISDFYGRTKVMIC
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHH
SVAATVIAAVAVFFLMSSDIMWHQLLGLTGLGILAGGVSGVSYIFIISLFTPEQRFTGIA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHEEHHEEEEECCCHHHCEEE
FSFNLGNAIFGGTAPTITTWLAGTIKHNYGPTLGHIAPAFYIMTTSTIFLLIMYFTYHWI
EEEECCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEYDNQQNFSNKH
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8550465 [H]