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The map label for this gene is sucB [H]

Identifier: 148284803

GI number: 148284803

Start: 1452865

End: 1454142

Strand: Direct

Name: sucB [H]

Synonym: OTBS_1491

Alternate gene names: 148284803

Gene position: 1452865-1454142 (Clockwise)

Preceding gene: 148284802

Following gene: 148284804

Centisome position: 68.3

GC content: 31.46

Gene sequence:

>1278_bases
ATGACAGAAAAAAATAAGGAAACAAATATAGTGTTGCCATCACTTGGAGAATCAGTATCAACTGGGACAATATCTAAATG
GCATAAAAAAGAAGGGGATATAGTCGCGCTAGATGAGAAAATAGTAGAGGTAGAAAGTGACAAAGTAGGAATAGATATTA
ACGCTAATGTACCTGGAAAAATAACTAAAATTTTAAAAAACGAAGGCGATAATGTTGAGGTTGGAGAAGTAATTTGTGTT
ATTAGATCAGATGTTTTACAGAAAGAAATTCATAGCAGTAAAAGTAGTGAGATTGATATAAATCTATCAATCTGCGAAGA
TATTATTTCTGCTAATAAATTGTCTCCTGCTGTTGCTAAAATGGTAGCAGAGCATAAAATTAATCCTGAGAATATTAGCG
GTAGTGGAAAAAATAATAGAATTACAAAAGGTGATATTATTGATGTTATAGATTCAAACTTAAATAATAACAATATAACA
TCAACAAAAGATATACAAACTCAGCAATTACAGATATCAACATTAATACGTGAGACATCAACGCAAACAGCATCAGTGTT
AACTGATGTAAAAGCTAGTAGAACAATAGAACGAGTGAAAATGACTAGATTGAGACGTACTATTGCTCAACGCTTAAAAG
ATTCTCAAAACAATGCTGCTATTCTATCTACCTTTAATGAAGTTGATATGTTTAATGTATCGGAATTAAGGAAAAAATAT
AAAGAAGAATTTGAAAAAAAACATGAAATTAAACTTGGTTTTATGTCATTTTTTGTTAAAGCAGCAACTGCAGCTTTGCA
AGAGCTTCCAATAATTAATGCTCAAGTTGATGGTTATGACATACTATATCACAATTACTGCGATATAGGAGTAGCAGTAA
GTACTAATTCCGGATTAGTTGTGCCAATAATAAGGAATGCTGAACATCTTTCTTTTGCTGAGATAGAAATGGAAATTTCT
CAGTTAGGTAAAAAAGCTAGAGAAGGAAATTTATCGATTAATGAACTTAGTGGAGGAACTTTCTCTATTACTAATGGAGG
AGTGTTTGGTTCATTGCTGTCAACCCCAATTATTAATCCGCCTCAATCTGCAATAATGGGAATGCATAAAATACAGGACA
GGCCTGTAGTAATTAATGGCACTATACAAATTAGGCCAATGATGTATATAGTATTATCATATGATCACAGAATTATTGAT
GGTAAGGAGGCAGTGACATTTTTAACTAAAGTAAAAAGCTATATAGAATCACCAGAAAGGCTATTGCTTAATATTTAA

Upstream 100 bases:

>100_bases
GTGCTTCAGCTCCTGCAGTAGGATATAGTTATCTTCATAATAAGCAGCAGCAGGAATTAATACAGTGCGCTTTTAGTATA
GATATAGGAGAAAGAATATA

Downstream 100 bases:

>100_bases
TTTTTATGAAAAAGAAAATAACTATTGTTATTATAGTATTAGTAGCTTTAATAACTGTTAATTTTGCTGCTGATGGATGC
GGAGAAAAGTCTATGGTATT

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTEKNKETNIVLPSLGESVSTGTISKWHKKEGDIVALDEKIVEVESDKVGIDINANVPGKITKILKNEGDNVEVGEVICV
IRSDVLQKEIHSSKSSEIDINLSICEDIISANKLSPAVAKMVAEHKINPENISGSGKNNRITKGDIIDVIDSNLNNNNIT
STKDIQTQQLQISTLIRETSTQTASVLTDVKASRTIERVKMTRLRRTIAQRLKDSQNNAAILSTFNEVDMFNVSELRKKY
KEEFEKKHEIKLGFMSFFVKAATAALQELPIINAQVDGYDILYHNYCDIGVAVSTNSGLVVPIIRNAEHLSFAEIEMEIS
QLGKKAREGNLSINELSGGTFSITNGGVFGSLLSTPIINPPQSAIMGMHKIQDRPVVINGTIQIRPMMYIVLSYDHRIID
GKEAVTFLTKVKSYIESPERLLLNI

Sequences:

>Translated_425_residues
MTEKNKETNIVLPSLGESVSTGTISKWHKKEGDIVALDEKIVEVESDKVGIDINANVPGKITKILKNEGDNVEVGEVICV
IRSDVLQKEIHSSKSSEIDINLSICEDIISANKLSPAVAKMVAEHKINPENISGSGKNNRITKGDIIDVIDSNLNNNNIT
STKDIQTQQLQISTLIRETSTQTASVLTDVKASRTIERVKMTRLRRTIAQRLKDSQNNAAILSTFNEVDMFNVSELRKKY
KEEFEKKHEIKLGFMSFFVKAATAALQELPIINAQVDGYDILYHNYCDIGVAVSTNSGLVVPIIRNAEHLSFAEIEMEIS
QLGKKAREGNLSINELSGGTFSITNGGVFGSLLSTPIINPPQSAIMGMHKIQDRPVVINGTIQIRPMMYIVLSYDHRIID
GKEAVTFLTKVKSYIESPERLLLNI
>Mature_424_residues
TEKNKETNIVLPSLGESVSTGTISKWHKKEGDIVALDEKIVEVESDKVGIDINANVPGKITKILKNEGDNVEVGEVICVI
RSDVLQKEIHSSKSSEIDINLSICEDIISANKLSPAVAKMVAEHKINPENISGSGKNNRITKGDIIDVIDSNLNNNNITS
TKDIQTQQLQISTLIRETSTQTASVLTDVKASRTIERVKMTRLRRTIAQRLKDSQNNAAILSTFNEVDMFNVSELRKKYK
EEFEKKHEIKLGFMSFFVKAATAALQELPIINAQVDGYDILYHNYCDIGVAVSTNSGLVVPIIRNAEHLSFAEIEMEISQ
LGKKAREGNLSINELSGGTFSITNGGVFGSLLSTPIINPPQSAIMGMHKIQDRPVVINGTIQIRPMMYIVLSYDHRIIDG
KEAVTFLTKVKSYIESPERLLLNI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=237, Percent_Identity=58.6497890295359, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI110671329, Length=421, Percent_Identity=29.9287410926366, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=28.4444444444444, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=28.4444444444444, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI31711992, Length=449, Percent_Identity=27.3942093541203, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI260898739, Length=165, Percent_Identity=36.969696969697, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1786946, Length=423, Percent_Identity=43.0260047281324, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1786305, Length=428, Percent_Identity=25.7009345794392, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=39.667458432304, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17560088, Length=437, Percent_Identity=27.9176201372998, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=432, Percent_Identity=28.2407407407407, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=27.9742765273312, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=230, Percent_Identity=56.0869565217391, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6324258, Length=458, Percent_Identity=27.7292576419214, Blast_Score=126, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=56.3876651982379, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI18859875, Length=446, Percent_Identity=27.3542600896861, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=30.3030303030303, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=30.3030303030303, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 47004; Mature: 46872

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKNKETNIVLPSLGESVSTGTISKWHKKEGDIVALDEKIVEVESDKVGIDINANVPGK
CCCCCCCCEEECCCCCCCCCCCCCHHHHCCCCCEEEECCEEEEECCCEEEEEECCCCCHH
ITKILKNEGDNVEVGEVICVIRSDVLQKEIHSSKSSEIDINLSICEDIISANKLSPAVAK
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCHHHHH
MVAEHKINPENISGSGKNNRITKGDIIDVIDSNLNNNNITSTKDIQTQQLQISTLIRETS
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
TQTASVLTDVKASRTIERVKMTRLRRTIAQRLKDSQNNAAILSTFNEVDMFNVSELRKKY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
KEEFEKKHEIKLGFMSFFVKAATAALQELPIINAQVDGYDILYHNYCDIGVAVSTNSGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCEEEEEECCCCEE
VPIIRNAEHLSFAEIEMEISQLGKKAREGNLSINELSGGTFSITNGGVFGSLLSTPIINP
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHCCCCCC
PQSAIMGMHKIQDRPVVINGTIQIRPMMYIVLSYDHRIIDGKEAVTFLTKVKSYIESPER
CHHHHHHHHHHCCCCEEEECEEEEEEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHH
LLLNI
HEECC
>Mature Secondary Structure 
TEKNKETNIVLPSLGESVSTGTISKWHKKEGDIVALDEKIVEVESDKVGIDINANVPGK
CCCCCCCEEECCCCCCCCCCCCCHHHHCCCCCEEEECCEEEEECCCEEEEEECCCCCHH
ITKILKNEGDNVEVGEVICVIRSDVLQKEIHSSKSSEIDINLSICEDIISANKLSPAVAK
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCHHHHH
MVAEHKINPENISGSGKNNRITKGDIIDVIDSNLNNNNITSTKDIQTQQLQISTLIRETS
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
TQTASVLTDVKASRTIERVKMTRLRRTIAQRLKDSQNNAAILSTFNEVDMFNVSELRKKY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
KEEFEKKHEIKLGFMSFFVKAATAALQELPIINAQVDGYDILYHNYCDIGVAVSTNSGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCEEEEEECCCCEE
VPIIRNAEHLSFAEIEMEISQLGKKAREGNLSINELSGGTFSITNGGVFGSLLSTPIINP
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHCCCCCC
PQSAIMGMHKIQDRPVVINGTIQIRPMMYIVLSYDHRIIDGKEAVTFLTKVKSYIESPER
CHHHHHHHHHHCCCCEEEECEEEEEEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHH
LLLNI
HEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA