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The map label for this gene is hemB [H]

Identifier: 148284678

GI number: 148284678

Start: 1193457

End: 1194470

Strand: Direct

Name: hemB [H]

Synonym: OTBS_1224

Alternate gene names: 148284678

Gene position: 1193457-1194470 (Clockwise)

Preceding gene: 148284674

Following gene: 148284679

Centisome position: 56.11

GC content: 32.05

Gene sequence:

>1014_bases
ATGGATTACTTGAGTTGCAAATTTGTAAGATTAAGACGTAATCGACAATATGAATGGCTAAGAGATTTAGTTGCAGAGAC
AAGCATTTTGTCTTCAGATTTAATTTTGCCGATTTTTATTACTGATGGTATGAATAAAGAAATAATAATTAACACTATGC
CTAGCGTTAAAATTGTTTCACCTGATTTAGCTTTGCAGAAAGTTGAGGAAGCTATTGATTATGGTATAAGAGCTGTTGCA
TTGTTTCCTAGTATTTCTGCAGCACAAAAAAGCAATGATGCAGCAGAAGCTTGTAATGCTAATAACCTTATTTGTCAAAC
TATTAGATTATTAAAAAGTAATAATAGGTTTAGAAAAGTAGGAATTATTGCTGATGTTGCACTGGATCCTTATACTATAC
ATGGCCATGACGGATTACTAACTGATGATTGTTTAGATATAGATAATGATAGGACTTTAGCAGTGCTATGCAAGCAATCA
CTAAAGTTAGCTGATGCTGGAGCTGATATAATTGCCCCATCCGATATGATGGATGGTAGGGTTAAGGCAATACGAGGATC
CTTAAATAGCAAAGGGTATTGTAATACTATTATTTTATCTTATGCAGTTAAATATGCATCAAATTTTTATGGACCATTTC
GTAATGTAGTAGGAGCAAAATTATCTTCTGGACCATCCTCAAAAAAAACTTATCAAATGGATTATCGTAATACTAGAGAA
GCATTAAAAGCAGTAGAAATTAATATTCAAGAAGGAGCAGATATGGTAGTAGTTAAGCCTGCTATGACATATTTAGATAT
AATTTCTAATGTTAAAAATAATTTTAATATTCCAGTTTTTGCTTATCAAACTAGCGGTGAATATTCTATGATAAAGTATG
CAGCTCTTAATGATTGCATGGACTTTGATCAAGCAATATTAGAATCAATTCAATGTATTAAAAGAGCAGGCGCAACAGCA
ATTTTTACTTATGCTGCGATTAAACTAGCAAGGTTTCTAGCAGCTAATCATTAG

Upstream 100 bases:

>100_bases
TTTTACTTTCCAGTATACTTAAAGTGTTGTGAGCACGCAATAAGATATTGCTTTGCATTATTGAATGATTTAATTGATTG
TTAAAAAGGATATTATTTTA

Downstream 100 bases:

>100_bases
ATATGATTTAGGTATAGATGATTTTTGCAAGTATTTTGAAAAAGGAATAAAAAAAATGTATGCTTCAAATCCTAAATACA
AAGGTAAAATGAGTTGTAAT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MDYLSCKFVRLRRNRQYEWLRDLVAETSILSSDLILPIFITDGMNKEIIINTMPSVKIVSPDLALQKVEEAIDYGIRAVA
LFPSISAAQKSNDAAEACNANNLICQTIRLLKSNNRFRKVGIIADVALDPYTIHGHDGLLTDDCLDIDNDRTLAVLCKQS
LKLADAGADIIAPSDMMDGRVKAIRGSLNSKGYCNTIILSYAVKYASNFYGPFRNVVGAKLSSGPSSKKTYQMDYRNTRE
ALKAVEINIQEGADMVVVKPAMTYLDIISNVKNNFNIPVFAYQTSGEYSMIKYAALNDCMDFDQAILESIQCIKRAGATA
IFTYAAIKLARFLAANH

Sequences:

>Translated_337_residues
MDYLSCKFVRLRRNRQYEWLRDLVAETSILSSDLILPIFITDGMNKEIIINTMPSVKIVSPDLALQKVEEAIDYGIRAVA
LFPSISAAQKSNDAAEACNANNLICQTIRLLKSNNRFRKVGIIADVALDPYTIHGHDGLLTDDCLDIDNDRTLAVLCKQS
LKLADAGADIIAPSDMMDGRVKAIRGSLNSKGYCNTIILSYAVKYASNFYGPFRNVVGAKLSSGPSSKKTYQMDYRNTRE
ALKAVEINIQEGADMVVVKPAMTYLDIISNVKNNFNIPVFAYQTSGEYSMIKYAALNDCMDFDQAILESIQCIKRAGATA
IFTYAAIKLARFLAANH
>Mature_337_residues
MDYLSCKFVRLRRNRQYEWLRDLVAETSILSSDLILPIFITDGMNKEIIINTMPSVKIVSPDLALQKVEEAIDYGIRAVA
LFPSISAAQKSNDAAEACNANNLICQTIRLLKSNNRFRKVGIIADVALDPYTIHGHDGLLTDDCLDIDNDRTLAVLCKQS
LKLADAGADIIAPSDMMDGRVKAIRGSLNSKGYCNTIILSYAVKYASNFYGPFRNVVGAKLSSGPSSKKTYQMDYRNTRE
ALKAVEINIQEGADMVVVKPAMTYLDIISNVKNNFNIPVFAYQTSGEYSMIKYAALNDCMDFDQAILESIQCIKRAGATA
IFTYAAIKLARFLAANH

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=312, Percent_Identity=35.5769230769231, Blast_Score=189, Evalue=4e-48,
Organism=Escherichia coli, GI87081728, Length=321, Percent_Identity=39.5638629283489, Blast_Score=220, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321398, Length=321, Percent_Identity=34.5794392523364, Blast_Score=170, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21358291, Length=317, Percent_Identity=33.1230283911672, Blast_Score=174, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37301; Mature: 37301

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLSCKFVRLRRNRQYEWLRDLVAETSILSSDLILPIFITDGMNKEIIINTMPSVKIVS
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCEEEEC
PDLALQKVEEAIDYGIRAVALFPSISAAQKSNDAAEACNANNLICQTIRLLKSNNRFRKV
CHHHHHHHHHHHHHCCEEEEECCCHHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEE
GIIADVALDPYTIHGHDGLLTDDCLDIDNDRTLAVLCKQSLKLADAGADIIAPSDMMDGR
EEEEEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCHHCCCH
VKAIRGSLNSKGYCNTIILSYAVKYASNFYGPFRNVVGAKLSSGPSSKKTYQMDYRNTRE
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEECHHHHHH
ALKAVEINIQEGADMVVVKPAMTYLDIISNVKNNFNIPVFAYQTSGEYSMIKYAALNDCM
HHHHHEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEHHHHHHHH
DFDQAILESIQCIKRAGATAIFTYAAIKLARFLAANH
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYLSCKFVRLRRNRQYEWLRDLVAETSILSSDLILPIFITDGMNKEIIINTMPSVKIVS
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCEEEEC
PDLALQKVEEAIDYGIRAVALFPSISAAQKSNDAAEACNANNLICQTIRLLKSNNRFRKV
CHHHHHHHHHHHHHCCEEEEECCCHHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEE
GIIADVALDPYTIHGHDGLLTDDCLDIDNDRTLAVLCKQSLKLADAGADIIAPSDMMDGR
EEEEEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCHHCCCH
VKAIRGSLNSKGYCNTIILSYAVKYASNFYGPFRNVVGAKLSSGPSSKKTYQMDYRNTRE
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEECHHHHHH
ALKAVEINIQEGADMVVVKPAMTYLDIISNVKNNFNIPVFAYQTSGEYSMIKYAALNDCM
HHHHHEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEHHHHHHHH
DFDQAILESIQCIKRAGATAIFTYAAIKLARFLAANH
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]