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The map label for this gene is gidA

Identifier: 148284568

GI number: 148284568

Start: 1003489

End: 1005357

Strand: Direct

Name: gidA

Synonym: OTBS_1017

Alternate gene names: 148284568

Gene position: 1003489-1005357 (Clockwise)

Preceding gene: 148284567

Following gene: 148284574

Centisome position: 47.18

GC content: 33.92

Gene sequence:

>1869_bases
ATGAATAAATTTGATGTTGTAGTAGTTGGTGGTGGCCATGCTGGCTGTGAAGCAGCTGCAGCAGCAGCACGAATTGGTGT
TAAAGTTGCATTAATTACTCTTAAACCTGAAAATCTTGGAGAAATGCCATGTAACCCAGCTATTGGTGGCCGTGGTAAAG
GTCACATAGTCAAAGAAGTTGATGCTCTTGATGGCCTTATTGGGTATATAGCAGATCAAGCTGGCATACACTATAAAATG
TTGAATCATACTAAAGGACCAGCTGTTTGGGGGCCTAGGGCACAAATAGATCGTGCGCTATATAAATCTGCAATGTACAG
TACAATAATGAATTATCCTAATTTAACTACAATATTTGCATCAGTAGAGGATATTAAAGTTATTGCTAATAAAGTTACTG
CAGTAATTGTCAATGGAAAAGAAATTTATTGTCAAAAGGTTATACTAACTACTGGTACTTTCTTATCTGGTGTAATTCAC
CGTGGTAAAGAGCAAATAAAAGCTGGACGCTTGGGAGAAAATGCATCTTATGGATTATCTAATACTTTATCAGAACTTGG
ATTGAGACTAGGTAGATTAAAAACTGGTACTCCGCCTAGAATTGATAGTAGAACCATAGATTACTCGAAATTAGAGGAGC
AACCAGGGGATTTAATTCCTACTCCATTTTCTGAAATTACTAAGAAGGTTTTAGTTCCGCAAATTAAGTGTTATATTACC
AGAACTAATGAAATAACACATAAAATTATAAAGGAAAATTTACATCTTTCTGCAATGTATAGCGGGCAAATTCAAGGAAC
AGGCCCAAGATATTGTCCATCAATAGAGGATAAAATTATAAGGTTTAGTCATAATGCGAGTCATCAAATCTTTTTAGAGC
CAGAAGGATTAAACAGTAACACTATCTATCCCAATGGAATATCAACATCTTTACCAAGTGATGTGCAAGAAAATATGATT
AGAACGATAGAAGGTTTGGAAAATTGCAAAATAATAGCATATGGCTACGCTATTGAATATGATTATGTTGATCCTAGACA
ACTAAAGCATAGCCTAGAAGTGAAATCTGTAGGTGGGCTATATTTAGCTGGACAGATTAATGGTACTACAGGTTATGAGG
AAGCAGCAGGGCAAGGCATCATGGCAGGAATAAATGCTGCACTAGCAGTAAAAAAGCAAGATCCATTTATCTTAGATAGA
ACAGATGCTTATATTGGAGTAATGATTGATGATTTAACAAACGGAATAGATGAACCTTACCGTATGTTTACTTCAAGATC
AGAATACAGATTAAGTATTAGAGCTGACAATGCTGACCAGCGTCTCACACCAAAAGCAATAGATCTTGGTTGTGTAAGTC
AGTTAAGACAGGAAGTATTTCAGAAAAAATTATCTAAATTAAATACTTTACGTGATTATGTTCAGTCACTAACCATTACT
CCTAAACAACTTCAAAATTGTGGTTATCAAATTTCACAGAATGGTATAGCGCGTACTGCGTTTAGCTTACTTGGGCTTCC
AAATTTTGGTATTAATATAGTAAAAGATATTTACCCTGAATTAAATCGATATGATAACAATTTATTATTATTACTAACTT
ATGAATCTAAATATCATGTATATTTAGAGCGTCAAAAAGAAGATATTGAGCTTTTTAAGCAAGAAGAAACATATCAAATT
CCTCAAGATTTGAACTATGACCAGATTCCTAGTTTATCAATTGAAGTACGAGAAAAGCTTAAGCAGTATCAACCTGAGAC
TATAAGAGCTGCAAAACATATAAATGGAGTAACTCCATCAGCAATTATGGCAATAATCATTTTTTTAAAAACCAAATATT
TGAATTCTTCCATAACTGATAAAAAATGA

Upstream 100 bases:

>100_bases
TACATAATGTTATTAGTTTAACTAGCGCATGTTAGGTAGTTGATTGTTACATCATCTATAATGAGCTAGCTTAGTTAGTT
TTTAGTAAAATGTTGAAAGA

Downstream 100 bases:

>100_bases
TTATATGTTATATAACTTTTGAATATTAGATAAGTACATAGCAATTTTAACACTAAATTAATTAATACAAGAGAACCATA
AACTAAAAATTAATAGCAGA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MNKFDVVVVGGGHAGCEAAAAAARIGVKVALITLKPENLGEMPCNPAIGGRGKGHIVKEVDALDGLIGYIADQAGIHYKM
LNHTKGPAVWGPRAQIDRALYKSAMYSTIMNYPNLTTIFASVEDIKVIANKVTAVIVNGKEIYCQKVILTTGTFLSGVIH
RGKEQIKAGRLGENASYGLSNTLSELGLRLGRLKTGTPPRIDSRTIDYSKLEEQPGDLIPTPFSEITKKVLVPQIKCYIT
RTNEITHKIIKENLHLSAMYSGQIQGTGPRYCPSIEDKIIRFSHNASHQIFLEPEGLNSNTIYPNGISTSLPSDVQENMI
RTIEGLENCKIIAYGYAIEYDYVDPRQLKHSLEVKSVGGLYLAGQINGTTGYEEAAGQGIMAGINAALAVKKQDPFILDR
TDAYIGVMIDDLTNGIDEPYRMFTSRSEYRLSIRADNADQRLTPKAIDLGCVSQLRQEVFQKKLSKLNTLRDYVQSLTIT
PKQLQNCGYQISQNGIARTAFSLLGLPNFGINIVKDIYPELNRYDNNLLLLLTYESKYHVYLERQKEDIELFKQEETYQI
PQDLNYDQIPSLSIEVREKLKQYQPETIRAAKHINGVTPSAIMAIIIFLKTKYLNSSITDKK

Sequences:

>Translated_622_residues
MNKFDVVVVGGGHAGCEAAAAAARIGVKVALITLKPENLGEMPCNPAIGGRGKGHIVKEVDALDGLIGYIADQAGIHYKM
LNHTKGPAVWGPRAQIDRALYKSAMYSTIMNYPNLTTIFASVEDIKVIANKVTAVIVNGKEIYCQKVILTTGTFLSGVIH
RGKEQIKAGRLGENASYGLSNTLSELGLRLGRLKTGTPPRIDSRTIDYSKLEEQPGDLIPTPFSEITKKVLVPQIKCYIT
RTNEITHKIIKENLHLSAMYSGQIQGTGPRYCPSIEDKIIRFSHNASHQIFLEPEGLNSNTIYPNGISTSLPSDVQENMI
RTIEGLENCKIIAYGYAIEYDYVDPRQLKHSLEVKSVGGLYLAGQINGTTGYEEAAGQGIMAGINAALAVKKQDPFILDR
TDAYIGVMIDDLTNGIDEPYRMFTSRSEYRLSIRADNADQRLTPKAIDLGCVSQLRQEVFQKKLSKLNTLRDYVQSLTIT
PKQLQNCGYQISQNGIARTAFSLLGLPNFGINIVKDIYPELNRYDNNLLLLLTYESKYHVYLERQKEDIELFKQEETYQI
PQDLNYDQIPSLSIEVREKLKQYQPETIRAAKHINGVTPSAIMAIIIFLKTKYLNSSITDKK
>Mature_622_residues
MNKFDVVVVGGGHAGCEAAAAAARIGVKVALITLKPENLGEMPCNPAIGGRGKGHIVKEVDALDGLIGYIADQAGIHYKM
LNHTKGPAVWGPRAQIDRALYKSAMYSTIMNYPNLTTIFASVEDIKVIANKVTAVIVNGKEIYCQKVILTTGTFLSGVIH
RGKEQIKAGRLGENASYGLSNTLSELGLRLGRLKTGTPPRIDSRTIDYSKLEEQPGDLIPTPFSEITKKVLVPQIKCYIT
RTNEITHKIIKENLHLSAMYSGQIQGTGPRYCPSIEDKIIRFSHNASHQIFLEPEGLNSNTIYPNGISTSLPSDVQENMI
RTIEGLENCKIIAYGYAIEYDYVDPRQLKHSLEVKSVGGLYLAGQINGTTGYEEAAGQGIMAGINAALAVKKQDPFILDR
TDAYIGVMIDDLTNGIDEPYRMFTSRSEYRLSIRADNADQRLTPKAIDLGCVSQLRQEVFQKKLSKLNTLRDYVQSLTIT
PKQLQNCGYQISQNGIARTAFSLLGLPNFGINIVKDIYPELNRYDNNLLLLLTYESKYHVYLERQKEDIELFKQEETYQI
PQDLNYDQIPSLSIEVREKLKQYQPETIRAAKHINGVTPSAIMAIIIFLKTKYLNSSITDKK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=630, Percent_Identity=47.6190476190476, Blast_Score=526, Evalue=1e-149,
Organism=Homo sapiens, GI19882217, Length=655, Percent_Identity=45.8015267175573, Blast_Score=513, Evalue=1e-145,
Organism=Homo sapiens, GI183227703, Length=670, Percent_Identity=44.7761194029851, Blast_Score=506, Evalue=1e-143,
Organism=Escherichia coli, GI2367273, Length=623, Percent_Identity=48.7961476725522, Blast_Score=550, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17534255, Length=615, Percent_Identity=41.1382113821138, Blast_Score=460, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6321202, Length=626, Percent_Identity=46.6453674121406, Blast_Score=545, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24658174, Length=631, Percent_Identity=46.1172741679873, Blast_Score=523, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_ORITB (A5CDS8)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248658.1
- ProteinModelPortal:   A5CDS8
- SMR:   A5CDS8
- STRING:   A5CDS8
- GeneID:   5219604
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1017
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   OTSU357244:OTBS_1017-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69131; Mature: 69131

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 122-122 BINDING 176-176 BINDING 365-365

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFDVVVVGGGHAGCEAAAAAARIGVKVALITLKPENLGEMPCNPAIGGRGKGHIVKEV
CCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
DALDGLIGYIADQAGIHYKMLNHTKGPAVWGPRAQIDRALYKSAMYSTIMNYPNLTTIFA
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEHH
SVEDIKVIANKVTAVIVNGKEIYCQKVILTTGTFLSGVIHRGKEQIKAGRLGENASYGLS
HHHHHHHHHCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCHH
NTLSELGLRLGRLKTGTPPRIDSRTIDYSKLEEQPGDLIPTPFSEITKKVLVPQIKCYIT
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEE
RTNEITHKIIKENLHLSAMYSGQIQGTGPRYCPSIEDKIIRFSHNASHQIFLEPEGLNSN
ECHHHHHHHHHCCCEEEEEEECEECCCCCCCCCCHHHHHEEEECCCCCEEEEECCCCCCC
TIYPNGISTSLPSDVQENMIRTIEGLENCKIIAYGYAIEYDYVDPRQLKHSLEVKSVGGL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHCCEEECCCE
YLAGQINGTTGYEEAAGQGIMAGINAALAVKKQDPFILDRTDAYIGVMIDDLTNGIDEPY
EEEEEECCCCCCHHHCCCCEEECCCEEEEEECCCCEEEECCCCEEEEEEHHHHCCCCHHH
RMFTSRSEYRLSIRADNADQRLTPKAIDLGCVSQLRQEVFQKKLSKLNTLRDYVQSLTIT
HHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKQLQNCGYQISQNGIARTAFSLLGLPNFGINIVKDIYPELNRYDNNLLLLLTYESKYHV
HHHHHHCCCEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEE
YLERQKEDIELFKQEETYQIPQDLNYDQIPSLSIEVREKLKQYQPETIRAAKHINGVTPS
EEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHH
AIMAIIIFLKTKYLNSSITDKK
HHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNKFDVVVVGGGHAGCEAAAAAARIGVKVALITLKPENLGEMPCNPAIGGRGKGHIVKEV
CCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
DALDGLIGYIADQAGIHYKMLNHTKGPAVWGPRAQIDRALYKSAMYSTIMNYPNLTTIFA
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEHH
SVEDIKVIANKVTAVIVNGKEIYCQKVILTTGTFLSGVIHRGKEQIKAGRLGENASYGLS
HHHHHHHHHCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCHH
NTLSELGLRLGRLKTGTPPRIDSRTIDYSKLEEQPGDLIPTPFSEITKKVLVPQIKCYIT
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEE
RTNEITHKIIKENLHLSAMYSGQIQGTGPRYCPSIEDKIIRFSHNASHQIFLEPEGLNSN
ECHHHHHHHHHCCCEEEEEEECEECCCCCCCCCCHHHHHEEEECCCCCEEEEECCCCCCC
TIYPNGISTSLPSDVQENMIRTIEGLENCKIIAYGYAIEYDYVDPRQLKHSLEVKSVGGL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHCCEEECCCE
YLAGQINGTTGYEEAAGQGIMAGINAALAVKKQDPFILDRTDAYIGVMIDDLTNGIDEPY
EEEEEECCCCCCHHHCCCCEEECCCEEEEEECCCCEEEECCCCEEEEEEHHHHCCCCHHH
RMFTSRSEYRLSIRADNADQRLTPKAIDLGCVSQLRQEVFQKKLSKLNTLRDYVQSLTIT
HHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKQLQNCGYQISQNGIARTAFSLLGLPNFGINIVKDIYPELNRYDNNLLLLLTYESKYHV
HHHHHHCCCEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEE
YLERQKEDIELFKQEETYQIPQDLNYDQIPSLSIEVREKLKQYQPETIRAAKHINGVTPS
EEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHH
AIMAIIIFLKTKYLNSSITDKK
HHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA