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The map label for this gene is glyS [H]

Identifier: 148261175

GI number: 148261175

Start: 2414989

End: 2417160

Strand: Reverse

Name: glyS [H]

Synonym: Acry_2185

Alternate gene names: 148261175

Gene position: 2417160-2414989 (Counterclockwise)

Preceding gene: 148261176

Following gene: 148261174

Centisome position: 71.32

GC content: 67.82

Gene sequence:

>2172_bases
ATGGCTGAGTTCTTTCTGGAGCTTTTCTCCGAGGAAATTCCGGCGCGGATGCAGGAAGCGGCGGCCGAGCAGCTCGCGAA
GCTGGTGGGCGCGCAGCTCGCGCCGCTGTCGCCGCGCGATATAAAGGCCTTCGGCGCCGCGCGGCGGATTGCCGTCGTGG
CGACGATCGACGCCGAGGTGCCGGAATCGGCGAGCGAACTGCGCGGGCCGAAGCTCGCCGCACCGCCGCAGGCGCTGGAG
GGGTTCCTGCGCAAGAATGGCGCGGCGCGGGAAGAGCTGGTCGAGGAGGACGGGTATTTCCTGCTCCGGCGGAACCTGCC
GTCGCGGCCGGCGGGCGAGCTGATCGCGGGGGAATTCGCGCAGGCGCTGGCGGGGTTCGGCTGGCCGAAATCGATGCGCT
GGGGGCAGAGCGGGGCGTTCACCTGGGTGCGGCCGTTGCGGCGGGTGGTGTGTCTGCTCGATGGCGAGGTGGTGCCGTTC
ACGCTCGGGCCGGTTGCGAGCGGGGATGAGAGCGAGGGGCACCGGTTTCTCGCGCCGGGCGCGTTCCGGGTAAGCTCCGC
CGCGCAATGGCGGGAGGAGCTGCGGGCGCGGTTCGTGATCGTCGATGCCGATGAGCGGCGGGAACGGATCAGGGCGGGGC
TGCGCGCGGCGGCCGGAGACAAAGGGCTCGGCGTTGCCGGGGATGCGGGGCTGCTGGACGAGGTCGCGGGGCTGGTGGAG
TGGCCGGTCTGCCTGATCGGGGCGATCGATCCGGGGCAGATGGCGCTGCCGCCGGAGGTGCGCGAACTGTCGATGAAGGT
GAACCAGCGCTATTTCGCCACGCGCGACGCGGCGGGGACTCCGGCGCCGTATTTCGCCTTCGTCGCCAATATCGCGGCGA
ATGACGGCGGGGCGGCGATCATCGCCGGCAATGAGCGGGTGCTGCGGGCAAGGCTCGCGGATGCGGAGCATTTCTGGGCG
CAGGACCGGAAGCAGACGCTGGAGTCGTGGCTGCCGAAGCTCGATGCGGTGACGTTCCACGCGAAGCTCGGCACCCAGCA
TGCGCGGGCCGGGCGGATCGAGGCGCTGGCGCGGGAGATCGCGCTGGCGCTGGGCGCGGATGCGGCGGCGGCGGATGCGG
CGGCGCGGGCCGGGCGGCTGTGCAAGGCGGATCTCGTGAGCGGGATGGTCGGCGAGTTCCCCGAGCTTCAGGGCATCATG
GGCGGGTATTACGCCGAGGCGTCGGGCGAGGGCGACGCGATCGCCGGGGCGATCCGCACGCATTACCAGCCGAAAGGCCC
GTCGGACGAGGTGCCTTCGGGGGTTGTGTCCGCCGCCGTGGCGCTGGCGGACAAGATCGATACGCTGGCCGGGTTCTTCG
TGATTGGCGAGAAGCCGACGGGTTCAGGTGATCCCTACGCCTTGCGCCGTGCCGCTCTCGGCGTGATCCGGATTGTGCTG
GTGAACCGCTTTGCCCTGCCTCTGCGGCAGATTCTGGACGTGGCTGTTGAGCGAATTAGCGAAAGCATTTTCGCGGCGGC
AGAAGAGACAACTGACAAGGCAATCGCCTACGCCCAAAGTCAGAACGTGCCAGTTGGCGCAAGAGCGAGGTTTTTTGCAA
GGGTAACAGTCTTAGATCCAGCTTCCGGGAAGCAGATCAATTCACGCGTTCCTGAAATCTTTGACTTTATCATTGATCGC
TTGCGCGTGCTTTTGAGAAGCGAAGGCAAGCGGTTCGACGTGCTGAACGCCGTTCTGGATACGGCGCGGGATGACGACCT
GACCCGGCTGATCGCGCGGACCGAGGCGCTGGGGGCGTTCCTGGCCACCGAGGACGGGGCGAACCTGCTCGCGGCGTATC
GGCGGAGTACAAACATCCTCAGGATCGAGGATGCGAAGGACGGGCCGCACGAGGGCACCATCGATCCCGGGTTGCTGGCG
GAGGCGGCGGAGATCGCGCTCGCGGATGCGCTGGAGGCGGTGGATCGCGAGGCCGTGCGGCGCCTGGCCGATGAGGATTT
CGCTGGCGCGATGCGGGGTTTCGCGGCGCTGCGCGCGCCGGTCGACGCGTTTTTCGATCATGTGACCGTGAACGCGACGG
GAGCAGAATTACGCCGCAATCGTTTGCGATTGCTGGCCCGCCTGCGCGACGTGATGCATCGCGTCGCCGATTTCTCGAAA
CTGGAAGGGTAA

Upstream 100 bases:

>100_bases
ACCGAGCGCGCCGCGTATATCGGCCGGGTGCGGGCGCTGGCGAAGGCGTGCGCGGAGGTCTGGGTCGCCGGCGAGCGGGC
CGAGGCGGAGGCGCAGAAGA

Downstream 100 bases:

>100_bases
TGCCCCGATGAGCAAATACGTCTACAGCTTCGGTCCCGGCGCCAGCGACGGTTCGGCGGCAATGCGCAACCTGCTTGGCG
GCAAGGGGGCGAACCTGGCC

Product: glycine--tRNA ligase

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MAEFFLELFSEEIPARMQEAAAEQLAKLVGAQLAPLSPRDIKAFGAARRIAVVATIDAEVPESASELRGPKLAAPPQALE
GFLRKNGAAREELVEEDGYFLLRRNLPSRPAGELIAGEFAQALAGFGWPKSMRWGQSGAFTWVRPLRRVVCLLDGEVVPF
TLGPVASGDESEGHRFLAPGAFRVSSAAQWREELRARFVIVDADERRERIRAGLRAAAGDKGLGVAGDAGLLDEVAGLVE
WPVCLIGAIDPGQMALPPEVRELSMKVNQRYFATRDAAGTPAPYFAFVANIAANDGGAAIIAGNERVLRARLADAEHFWA
QDRKQTLESWLPKLDAVTFHAKLGTQHARAGRIEALAREIALALGADAAAADAAARAGRLCKADLVSGMVGEFPELQGIM
GGYYAEASGEGDAIAGAIRTHYQPKGPSDEVPSGVVSAAVALADKIDTLAGFFVIGEKPTGSGDPYALRRAALGVIRIVL
VNRFALPLRQILDVAVERISESIFAAAEETTDKAIAYAQSQNVPVGARARFFARVTVLDPASGKQINSRVPEIFDFIIDR
LRVLLRSEGKRFDVLNAVLDTARDDDLTRLIARTEALGAFLATEDGANLLAAYRRSTNILRIEDAKDGPHEGTIDPGLLA
EAAEIALADALEAVDREAVRRLADEDFAGAMRGFAALRAPVDAFFDHVTVNATGAELRRNRLRLLARLRDVMHRVADFSK
LEG

Sequences:

>Translated_723_residues
MAEFFLELFSEEIPARMQEAAAEQLAKLVGAQLAPLSPRDIKAFGAARRIAVVATIDAEVPESASELRGPKLAAPPQALE
GFLRKNGAAREELVEEDGYFLLRRNLPSRPAGELIAGEFAQALAGFGWPKSMRWGQSGAFTWVRPLRRVVCLLDGEVVPF
TLGPVASGDESEGHRFLAPGAFRVSSAAQWREELRARFVIVDADERRERIRAGLRAAAGDKGLGVAGDAGLLDEVAGLVE
WPVCLIGAIDPGQMALPPEVRELSMKVNQRYFATRDAAGTPAPYFAFVANIAANDGGAAIIAGNERVLRARLADAEHFWA
QDRKQTLESWLPKLDAVTFHAKLGTQHARAGRIEALAREIALALGADAAAADAAARAGRLCKADLVSGMVGEFPELQGIM
GGYYAEASGEGDAIAGAIRTHYQPKGPSDEVPSGVVSAAVALADKIDTLAGFFVIGEKPTGSGDPYALRRAALGVIRIVL
VNRFALPLRQILDVAVERISESIFAAAEETTDKAIAYAQSQNVPVGARARFFARVTVLDPASGKQINSRVPEIFDFIIDR
LRVLLRSEGKRFDVLNAVLDTARDDDLTRLIARTEALGAFLATEDGANLLAAYRRSTNILRIEDAKDGPHEGTIDPGLLA
EAAEIALADALEAVDREAVRRLADEDFAGAMRGFAALRAPVDAFFDHVTVNATGAELRRNRLRLLARLRDVMHRVADFSK
LEG
>Mature_722_residues
AEFFLELFSEEIPARMQEAAAEQLAKLVGAQLAPLSPRDIKAFGAARRIAVVATIDAEVPESASELRGPKLAAPPQALEG
FLRKNGAAREELVEEDGYFLLRRNLPSRPAGELIAGEFAQALAGFGWPKSMRWGQSGAFTWVRPLRRVVCLLDGEVVPFT
LGPVASGDESEGHRFLAPGAFRVSSAAQWREELRARFVIVDADERRERIRAGLRAAAGDKGLGVAGDAGLLDEVAGLVEW
PVCLIGAIDPGQMALPPEVRELSMKVNQRYFATRDAAGTPAPYFAFVANIAANDGGAAIIAGNERVLRARLADAEHFWAQ
DRKQTLESWLPKLDAVTFHAKLGTQHARAGRIEALAREIALALGADAAAADAAARAGRLCKADLVSGMVGEFPELQGIMG
GYYAEASGEGDAIAGAIRTHYQPKGPSDEVPSGVVSAAVALADKIDTLAGFFVIGEKPTGSGDPYALRRAALGVIRIVLV
NRFALPLRQILDVAVERISESIFAAAEETTDKAIAYAQSQNVPVGARARFFARVTVLDPASGKQINSRVPEIFDFIIDRL
RVLLRSEGKRFDVLNAVLDTARDDDLTRLIARTEALGAFLATEDGANLLAAYRRSTNILRIEDAKDGPHEGTIDPGLLAE
AAEIALADALEAVDREAVRRLADEDFAGAMRGFAALRAPVDAFFDHVTVNATGAELRRNRLRLLARLRDVMHRVADFSKL
EG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=734, Percent_Identity=36.5122615803815, Blast_Score=366, Evalue=1e-102,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 77711; Mature: 77580

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFFLELFSEEIPARMQEAAAEQLAKLVGAQLAPLSPRDIKAFGAARRIAVVATIDAEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEEEECCCC
PESASELRGPKLAAPPQALEGFLRKNGAAREELVEEDGYFLLRRNLPSRPAGELIAGEFA
CCCHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHH
QALAGFGWPKSMRWGQSGAFTWVRPLRRVVCLLDGEVVPFTLGPVASGDESEGHRFLAPG
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEECCC
AFRVSSAAQWREELRARFVIVDADERRERIRAGLRAAAGDKGLGVAGDAGLLDEVAGLVE
CHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
WPVCLIGAIDPGQMALPPEVRELSMKVNQRYFATRDAAGTPAPYFAFVANIAANDGGAAI
CCHHEEECCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCCCEEE
IAGNERVLRARLADAEHFWAQDRKQTLESWLPKLDAVTFHAKLGTQHARAGRIEALAREI
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEHHHCCCHHHHHHHHHHHHHH
ALALGADAAAADAAARAGRLCKADLVSGMVGEFPELQGIMGGYYAEASGEGDAIAGAIRT
HHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCEEECCCCCCCHHHHHHHH
HYQPKGPSDEVPSGVVSAAVALADKIDTLAGFFVIGEKPTGSGDPYALRRAALGVIRIVL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
VNRFALPLRQILDVAVERISESIFAAAEETTDKAIAYAQSQNVPVGARARFFARVTVLDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECC
ASGKQINSRVPEIFDFIIDRLRVLLRSEGKRFDVLNAVLDTARDDDLTRLIARTEALGAF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LATEDGANLLAAYRRSTNILRIEDAKDGPHEGTIDPGLLAEAAEIALADALEAVDREAVR
HCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLADEDFAGAMRGFAALRAPVDAFFDHVTVNATGAELRRNRLRLLARLRDVMHRVADFSK
HHHCCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEG
CCC
>Mature Secondary Structure 
AEFFLELFSEEIPARMQEAAAEQLAKLVGAQLAPLSPRDIKAFGAARRIAVVATIDAEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEEEECCCC
PESASELRGPKLAAPPQALEGFLRKNGAAREELVEEDGYFLLRRNLPSRPAGELIAGEFA
CCCHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHH
QALAGFGWPKSMRWGQSGAFTWVRPLRRVVCLLDGEVVPFTLGPVASGDESEGHRFLAPG
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEECCC
AFRVSSAAQWREELRARFVIVDADERRERIRAGLRAAAGDKGLGVAGDAGLLDEVAGLVE
CHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
WPVCLIGAIDPGQMALPPEVRELSMKVNQRYFATRDAAGTPAPYFAFVANIAANDGGAAI
CCHHEEECCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCCCEEE
IAGNERVLRARLADAEHFWAQDRKQTLESWLPKLDAVTFHAKLGTQHARAGRIEALAREI
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEHHHCCCHHHHHHHHHHHHHH
ALALGADAAAADAAARAGRLCKADLVSGMVGEFPELQGIMGGYYAEASGEGDAIAGAIRT
HHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCEEECCCCCCCHHHHHHHH
HYQPKGPSDEVPSGVVSAAVALADKIDTLAGFFVIGEKPTGSGDPYALRRAALGVIRIVL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
VNRFALPLRQILDVAVERISESIFAAAEETTDKAIAYAQSQNVPVGARARFFARVTVLDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECC
ASGKQINSRVPEIFDFIIDRLRVLLRSEGKRFDVLNAVLDTARDDDLTRLIARTEALGAF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LATEDGANLLAAYRRSTNILRIEDAKDGPHEGTIDPGLLAEAAEIALADALEAVDREAVR
HCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLADEDFAGAMRGFAALRAPVDAFFDHVTVNATGAELRRNRLRLLARLRDVMHRVADFSK
HHHCCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA