Click here to switch to the map view.

The map label for this gene is ppdK [H]

Identifier: 148261174

GI number: 148261174

Start: 2412315

End: 2414981

Strand: Reverse

Name: ppdK [H]

Synonym: Acry_2184

Alternate gene names: 148261174

Gene position: 2414981-2412315 (Counterclockwise)

Preceding gene: 148261175

Following gene: 148261169

Centisome position: 71.25

GC content: 69.44

Gene sequence:

>2667_bases
ATGAGCAAATACGTCTACAGCTTCGGTCCCGGCGCCAGCGACGGTTCGGCGGCAATGCGCAACCTGCTTGGCGGCAAGGG
GGCGAACCTGGCCGAGATGGCGAGCATCGGCCTGCCGGTGCCGCCGGGTTTCACCATCACCACCGAGGTGTGCACCGCGT
TCTACGACAATGACCGCGCCTATCCGGAAGGGCTGCGCGAGCAGGTGGACGCGGCGCTGGCGAAGATCGAGGCGGAGGTG
GGGCGGCGCTTCGGCGACAAGCACAACCCGCTGCTGGTGTCGGTGCGTTCGGGCGCGCGGGTGTCGATGCCGGGGATGAT
GGATACCGTGCTCAATCTCGGGCTCAACGACGTGACGGTGCAGGGCCTGGCCGAGGGGGCGGGGGATGCGCGCTTCGCCT
GGGATTCGTATCGGCGGTTCATCCAGATGTACGGCTCGGTGGTGCTGGGTGTGGCGCATCACCGGTTCGAGGAGATCATC
GAGAACGTGAAGCTCGATGAGGGGGCGACCGAGGACACCCAGCTTTCGGCGCAGGACTGGCACCGCGTGGTCGAGGCCTA
CAAGGACGTGGTGCAGGCCGAGACCGGCCATCCGTTCCCGCAGGACCCGCACGACCAGCTCTGGGGCGCGGTGGGGGCGG
TGTTCGGCTCGTGGATGAACCAGCGGGCGATCACCTATCGGCGGCTGCACGAGATCCCGGCCGACTGGGGCACGGCGGTG
AACGTGCAGGCGATGGTGTTCGGCAACATGGGCGAGGATTGCGCGACCGGCGTGTGCTTCACCCGCGACCCATCGACCGG
GGTGAACGAGTTCTACGGCGAATATCTGCCGAACGCGCAGGGCGAGGACGTGGTGGCGGGCATCCGCACGCCGCGGCCGC
TGTCGAAATCCTATGCGAAGCCGGGCGAGATCAGCCTGGAGGAGGCGATGCCGCAGGCCTATGCCGAGCTGGTGCGGGTG
CGCGAGACGCTGGAGCGGCATTACCGCGACATGCAGGACATCGAATTCACCATCCAGCGGCAGAAGCTGTACATGTTGCA
GACGCGCAACGGCAAGCGGACCGCGCCGGCCAGCGTGCGGATCGCGGTGGAGATGGCGGAGTCGGGGCTGATCGACCAGC
GCGAGGCGGTGACGCGGGTGAACCCGCAATCGCTCGACCAGCTGCTGCATCCGACGCTCGATCCGAAGGCGAAGAAGACG
CTGCTGGCGCGCGGGCTGCCGGCCTCGCCGGGGGCGGCGTCGGGCATCGTGGTGTTCTCGGCCGACGAGGCGGAGATGCG
GGCGCAGAAGGGCGAGGCGGTGATCCTGGTGCGGATCGAGACCAGCCCCGAGGATATTCACGGGATGCAAGCGGCGCGCG
GCATCCTGACCACGCGGGGCGGGATGACCAGCCACGCGGCGGTGGTCGCGCGCGGGATGGGGCGGCCCTGTGTTGCGGGT
GCCGGCGGCATTCACGTCGATTACGGTGCGAAGACGCTCGCCGCCGGCGGGGTGGTCGTGCGCGCGGGCGACACGATCAC
GCTGGACGGGGCGACGGGCGAGATCTTCACCGGCTTCGTGCCGATGATCGAGCCGCAGCTGTCGGGCGATTTCGCCAAGC
TGATGGGCTGGGCCGACGCGGTGCGCCGGCTGCGGGTGCGGGCGAACGCGGAGACGCCGCTGGACGCCGAGACGGCGCGC
AAGTTCGGCGCCGAGGGGATCGGCCTGTCGCGCACCGAGCACATGTTCTTCGACCCGGAGCGGATCGGCGCGGTGCGGCA
GATGATCATGGCGCATGACGAGGCCGGACGGCGGGCGGCGCTGGCGAAGCTGCTGCCGTATCAGCGGCAGGATTTCGTGG
CGTTGTTCCGCATCATGGCGGGGCTGCCGGTGACGATCCGCCTGCTCGACCCGCCGCTGCACGAATTCCTGCCGCATGGC
GAGGCGGAGCTGGAGGAAGTGGCGGCGGCGCTGGGCATGGGCGTCGAGGCGATGCGCCAGCGCGCGGCCGAGCTTTCCGA
GGCGAACCCGATGCTCGGGCATCGCGGCTGCCGCCTCGGCATTTCGTTCCCGGAAATCTACGAGATGCAGGCGCGGGCGA
TCTTCGAGGCGGCGCTGGAGGTGGAGACCGAGACCGGCAAGGCGCCGGTGCCGGAGATCATGATTCCGCTGGTGGGGATG
AAGCGCGAGCTGGACATCACCCGCGGGCAGGTGGAGCGCGTGGCCAGCGCGGTGTTCGACGAGGCGGGGCGGGTGCTGGA
CTACAGCATCGGCACGATGATCGAGCTGCCGCGCGCGGCGCTGACGGCGGACACGATCGCGCAGTCCGCCGATTTCTTCT
CCTTCGGCACCAACGACCTGACGCAGACGGTGTTCGGCCTGTCGCGCGACGATGCCGGCAAGTTCCTGCCGAAATATGTC
GAGAGCGGAATCCTGCCGAAGGACCCGTTCGTCTCGCTCGACATCGAGGGCGTCGGCGGGATGATCCGCATCGCCGCCGA
GCGCGGGCGGGGCACCAAGGGCGGGCTCAAGCTCGGGATCTGCGGCGAGCATGGCGGCGATCCGGCCTCGATCGCGTTCT
GCGAGGAGATCGGGCTCGATTACGTGTCGTGCAGCCCGTATCGGGTGCCGGTGGCGCGGCTGGCGGCGCAGGCGGCGATC
GGCGCGGGCGGCGACCGGACGGCGTAA

Upstream 100 bases:

>100_bases
GGAGCAGAATTACGCCGCAATCGTTTGCGATTGCTGGCCCGCCTGCGCGACGTGATGCATCGCGTCGCCGATTTCTCGAA
ACTGGAAGGGTAATGCCCCG

Downstream 100 bases:

>100_bases
GCCGGATTTTTGCTCCGCCGACCGGATGGCGGAGCAGAAATACGGTCTGAGGCGCGCTGGAGCGGGTGTCTGGTCTGGCG
CGCGGGCGGGCGCAAAAACG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 888; Mature: 887

Protein sequence:

>888_residues
MSKYVYSFGPGASDGSAAMRNLLGGKGANLAEMASIGLPVPPGFTITTEVCTAFYDNDRAYPEGLREQVDAALAKIEAEV
GRRFGDKHNPLLVSVRSGARVSMPGMMDTVLNLGLNDVTVQGLAEGAGDARFAWDSYRRFIQMYGSVVLGVAHHRFEEII
ENVKLDEGATEDTQLSAQDWHRVVEAYKDVVQAETGHPFPQDPHDQLWGAVGAVFGSWMNQRAITYRRLHEIPADWGTAV
NVQAMVFGNMGEDCATGVCFTRDPSTGVNEFYGEYLPNAQGEDVVAGIRTPRPLSKSYAKPGEISLEEAMPQAYAELVRV
RETLERHYRDMQDIEFTIQRQKLYMLQTRNGKRTAPASVRIAVEMAESGLIDQREAVTRVNPQSLDQLLHPTLDPKAKKT
LLARGLPASPGAASGIVVFSADEAEMRAQKGEAVILVRIETSPEDIHGMQAARGILTTRGGMTSHAAVVARGMGRPCVAG
AGGIHVDYGAKTLAAGGVVVRAGDTITLDGATGEIFTGFVPMIEPQLSGDFAKLMGWADAVRRLRVRANAETPLDAETAR
KFGAEGIGLSRTEHMFFDPERIGAVRQMIMAHDEAGRRAALAKLLPYQRQDFVALFRIMAGLPVTIRLLDPPLHEFLPHG
EAELEEVAAALGMGVEAMRQRAAELSEANPMLGHRGCRLGISFPEIYEMQARAIFEAALEVETETGKAPVPEIMIPLVGM
KRELDITRGQVERVASAVFDEAGRVLDYSIGTMIELPRAALTADTIAQSADFFSFGTNDLTQTVFGLSRDDAGKFLPKYV
ESGILPKDPFVSLDIEGVGGMIRIAAERGRGTKGGLKLGICGEHGGDPASIAFCEEIGLDYVSCSPYRVPVARLAAQAAI
GAGGDRTA

Sequences:

>Translated_888_residues
MSKYVYSFGPGASDGSAAMRNLLGGKGANLAEMASIGLPVPPGFTITTEVCTAFYDNDRAYPEGLREQVDAALAKIEAEV
GRRFGDKHNPLLVSVRSGARVSMPGMMDTVLNLGLNDVTVQGLAEGAGDARFAWDSYRRFIQMYGSVVLGVAHHRFEEII
ENVKLDEGATEDTQLSAQDWHRVVEAYKDVVQAETGHPFPQDPHDQLWGAVGAVFGSWMNQRAITYRRLHEIPADWGTAV
NVQAMVFGNMGEDCATGVCFTRDPSTGVNEFYGEYLPNAQGEDVVAGIRTPRPLSKSYAKPGEISLEEAMPQAYAELVRV
RETLERHYRDMQDIEFTIQRQKLYMLQTRNGKRTAPASVRIAVEMAESGLIDQREAVTRVNPQSLDQLLHPTLDPKAKKT
LLARGLPASPGAASGIVVFSADEAEMRAQKGEAVILVRIETSPEDIHGMQAARGILTTRGGMTSHAAVVARGMGRPCVAG
AGGIHVDYGAKTLAAGGVVVRAGDTITLDGATGEIFTGFVPMIEPQLSGDFAKLMGWADAVRRLRVRANAETPLDAETAR
KFGAEGIGLSRTEHMFFDPERIGAVRQMIMAHDEAGRRAALAKLLPYQRQDFVALFRIMAGLPVTIRLLDPPLHEFLPHG
EAELEEVAAALGMGVEAMRQRAAELSEANPMLGHRGCRLGISFPEIYEMQARAIFEAALEVETETGKAPVPEIMIPLVGM
KRELDITRGQVERVASAVFDEAGRVLDYSIGTMIELPRAALTADTIAQSADFFSFGTNDLTQTVFGLSRDDAGKFLPKYV
ESGILPKDPFVSLDIEGVGGMIRIAAERGRGTKGGLKLGICGEHGGDPASIAFCEEIGLDYVSCSPYRVPVARLAAQAAI
GAGGDRTA
>Mature_887_residues
SKYVYSFGPGASDGSAAMRNLLGGKGANLAEMASIGLPVPPGFTITTEVCTAFYDNDRAYPEGLREQVDAALAKIEAEVG
RRFGDKHNPLLVSVRSGARVSMPGMMDTVLNLGLNDVTVQGLAEGAGDARFAWDSYRRFIQMYGSVVLGVAHHRFEEIIE
NVKLDEGATEDTQLSAQDWHRVVEAYKDVVQAETGHPFPQDPHDQLWGAVGAVFGSWMNQRAITYRRLHEIPADWGTAVN
VQAMVFGNMGEDCATGVCFTRDPSTGVNEFYGEYLPNAQGEDVVAGIRTPRPLSKSYAKPGEISLEEAMPQAYAELVRVR
ETLERHYRDMQDIEFTIQRQKLYMLQTRNGKRTAPASVRIAVEMAESGLIDQREAVTRVNPQSLDQLLHPTLDPKAKKTL
LARGLPASPGAASGIVVFSADEAEMRAQKGEAVILVRIETSPEDIHGMQAARGILTTRGGMTSHAAVVARGMGRPCVAGA
GGIHVDYGAKTLAAGGVVVRAGDTITLDGATGEIFTGFVPMIEPQLSGDFAKLMGWADAVRRLRVRANAETPLDAETARK
FGAEGIGLSRTEHMFFDPERIGAVRQMIMAHDEAGRRAALAKLLPYQRQDFVALFRIMAGLPVTIRLLDPPLHEFLPHGE
AELEEVAAALGMGVEAMRQRAAELSEANPMLGHRGCRLGISFPEIYEMQARAIFEAALEVETETGKAPVPEIMIPLVGMK
RELDITRGQVERVASAVFDEAGRVLDYSIGTMIELPRAALTADTIAQSADFFSFGTNDLTQTVFGLSRDDAGKFLPKYVE
SGILPKDPFVSLDIEGVGGMIRIAAERGRGTKGGLKLGICGEHGGDPASIAFCEEIGLDYVSCSPYRVPVARLAAQAAIG
AGGDRTA

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=459, Percent_Identity=26.797385620915, Blast_Score=115, Evalue=2e-26,
Organism=Escherichia coli, GI1787994, Length=706, Percent_Identity=25.4957507082153, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789193, Length=462, Percent_Identity=26.8398268398268, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788726, Length=463, Percent_Identity=24.1900647948164, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 95833; Mature: 95702

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYVYSFGPGASDGSAAMRNLLGGKGANLAEMASIGLPVPPGFTITTEVCTAFYDNDRA
CCCEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCC
YPEGLREQVDAALAKIEAEVGRRFGDKHNPLLVSVRSGARVSMPGMMDTVLNLGLNDVTV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCCEEH
QGLAEGAGDARFAWDSYRRFIQMYGSVVLGVAHHRFEEIIENVKLDEGATEDTQLSAQDW
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
HRVVEAYKDVVQAETGHPFPQDPHDQLWGAVGAVFGSWMNQRAITYRRLHEIPADWGTAV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
NVQAMVFGNMGEDCATGVCFTRDPSTGVNEFYGEYLPNAQGEDVVAGIRTPRPLSKSYAK
EEEEEEECCCCCHHHCCEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCC
PGEISLEEAMPQAYAELVRVRETLERHYRDMQDIEFTIQRQKLYMLQTRNGKRTAPASVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEE
IAVEMAESGLIDQREAVTRVNPQSLDQLLHPTLDPKAKKTLLARGLPASPGAASGIVVFS
EEEEEHHCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEE
ADEAEMRAQKGEAVILVRIETSPEDIHGMQAARGILTTRGGMTSHAAVVARGMGRPCVAG
CCHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCCCCC
AGGIHVDYGAKTLAAGGVVVRAGDTITLDGATGEIFTGFVPMIEPQLSGDFAKLMGWADA
CCCEEEECCCHHHCCCCEEEECCCEEEECCCCCCHHHCCHHHCCCCCCCCHHHHHHHHHH
VRRLRVRANAETPLDAETARKFGAEGIGLSRTEHMFFDPERIGAVRQMIMAHDEAGRRAA
HHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCHHHH
LAKLLPYQRQDFVALFRIMAGLPVTIRLLDPPLHEFLPHGEAELEEVAAALGMGVEAMRQ
HHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHHHHH
RAAELSEANPMLGHRGCRLGISFPEIYEMQARAIFEAALEVETETGKAPVPEIMIPLVGM
HHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHCCC
KRELDITRGQVERVASAVFDEAGRVLDYSIGTMIELPRAALTADTIAQSADFFSFGTNDL
CHHCCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHCCHHHHHHHHHHHCCCHHCCCCHHH
TQTVFGLSRDDAGKFLPKYVESGILPKDPFVSLDIEGVGGMIRIAAERGRGTKGGLKLGI
HHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEE
CGEHGGDPASIAFCEEIGLDYVSCSPYRVPVARLAAQAAIGAGGDRTA
CCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SKYVYSFGPGASDGSAAMRNLLGGKGANLAEMASIGLPVPPGFTITTEVCTAFYDNDRA
CCEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCC
YPEGLREQVDAALAKIEAEVGRRFGDKHNPLLVSVRSGARVSMPGMMDTVLNLGLNDVTV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCCEEH
QGLAEGAGDARFAWDSYRRFIQMYGSVVLGVAHHRFEEIIENVKLDEGATEDTQLSAQDW
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
HRVVEAYKDVVQAETGHPFPQDPHDQLWGAVGAVFGSWMNQRAITYRRLHEIPADWGTAV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
NVQAMVFGNMGEDCATGVCFTRDPSTGVNEFYGEYLPNAQGEDVVAGIRTPRPLSKSYAK
EEEEEEECCCCCHHHCCEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCC
PGEISLEEAMPQAYAELVRVRETLERHYRDMQDIEFTIQRQKLYMLQTRNGKRTAPASVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEE
IAVEMAESGLIDQREAVTRVNPQSLDQLLHPTLDPKAKKTLLARGLPASPGAASGIVVFS
EEEEEHHCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEE
ADEAEMRAQKGEAVILVRIETSPEDIHGMQAARGILTTRGGMTSHAAVVARGMGRPCVAG
CCHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCCCCC
AGGIHVDYGAKTLAAGGVVVRAGDTITLDGATGEIFTGFVPMIEPQLSGDFAKLMGWADA
CCCEEEECCCHHHCCCCEEEECCCEEEECCCCCCHHHCCHHHCCCCCCCCHHHHHHHHHH
VRRLRVRANAETPLDAETARKFGAEGIGLSRTEHMFFDPERIGAVRQMIMAHDEAGRRAA
HHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCHHHH
LAKLLPYQRQDFVALFRIMAGLPVTIRLLDPPLHEFLPHGEAELEEVAAALGMGVEAMRQ
HHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHHHHH
RAAELSEANPMLGHRGCRLGISFPEIYEMQARAIFEAALEVETETGKAPVPEIMIPLVGM
HHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHCCC
KRELDITRGQVERVASAVFDEAGRVLDYSIGTMIELPRAALTADTIAQSADFFSFGTNDL
CHHCCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHCCHHHHHHHHHHHCCCHHCCCCHHH
TQTVFGLSRDDAGKFLPKYVESGILPKDPFVSLDIEGVGGMIRIAAERGRGTKGGLKLGI
HHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEE
CGEHGGDPASIAFCEEIGLDYVSCSPYRVPVARLAAQAAIGAGGDRTA
CCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]