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The map label for this gene is trmD [H]

Identifier: 148261142

GI number: 148261142

Start: 2375812

End: 2376516

Strand: Reverse

Name: trmD [H]

Synonym: Acry_2151

Alternate gene names: 148261142

Gene position: 2376516-2375812 (Counterclockwise)

Preceding gene: 148261143

Following gene: 148261141

Centisome position: 70.12

GC content: 72.34

Gene sequence:

>705_bases
ATGAGCTTCCGCGCCACGATCATCACCCTGTTCCCCGAGATGTTTCCGGGGCCGCTCGGCCAGTCGCTGGCCGGACGCGC
GCTGGAGACGGGCATCTGGGCGCTTGATTGCGTGAACCTGCGCGATTTCGGCCTCGGCCGGCATCGCGCGGTGGACGACA
CGCCGTTCGGCGGCGGCGCGGGCATGGTGCTGCGGCCGGACGTGCTGGATGCCGCGATCGGCCAGGCGCGGGACGGGCGA
CCGTTGATCGCCCTGACGCCGCGCGGCGCGCCGCTGACCCAGGCGCGCATCCGCCGGCTTGCCGAAGGGCCGGGTGCCGT
GCTGCTCTGCGGCCGGTTCGAGGGGTTCGACCAGCGCGCCCTCGATGCCAACGAAGCCGAGGAGATCAGCCTCGGCGATT
TCGTGCTCTCGGGCGGCGAGATCGCGGCGCTCGCGCTGCTCGATGCCACCATCCGCCTGCTGCCGGGCGTGATGGGGGCG
GCGGAGAGCGCCGAGGAGGAAAGCTTCTCGGACGGCACGCTGCTCGAATATCCGCATTATACCCGCCCGGCCGTGTGGCA
GGGGCGGGCGGTGCCGGAGGCGCTGCTCTCCGGCCATCATGCCGCCATCGCCGCCTGGCGCCGCGCCGAGGCCGAGGCCG
TGACCAGGCGGCGCAGACCAGATCTATGGGCCCGGCACATTGCCAGCCAGCCGGCTCTTCCGTAA

Upstream 100 bases:

>100_bases
ACGGCTCGCTGCTGCCCTTCACCCGCGCGGTGGTGCCGGAGATCGACCTGCCGGGCGGCCGCGCCGTGGTGGTGCGGCCG
GTCGAGGTGGAGATGCGGGA

Downstream 100 bases:

>100_bases
ATGAACTCCCCCGAAAGGATGCCAGACATGAACGTGATCCAGACCATCGACGCCGAGCAGATCGGCAAGCTCGCGGAAGC
CCGCGCCGTGCCGGACTTCA

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSFRATIITLFPEMFPGPLGQSLAGRALETGIWALDCVNLRDFGLGRHRAVDDTPFGGGAGMVLRPDVLDAAIGQARDGR
PLIALTPRGAPLTQARIRRLAEGPGAVLLCGRFEGFDQRALDANEAEEISLGDFVLSGGEIAALALLDATIRLLPGVMGA
AESAEEESFSDGTLLEYPHYTRPAVWQGRAVPEALLSGHHAAIAAWRRAEAEAVTRRRRPDLWARHIASQPALP

Sequences:

>Translated_234_residues
MSFRATIITLFPEMFPGPLGQSLAGRALETGIWALDCVNLRDFGLGRHRAVDDTPFGGGAGMVLRPDVLDAAIGQARDGR
PLIALTPRGAPLTQARIRRLAEGPGAVLLCGRFEGFDQRALDANEAEEISLGDFVLSGGEIAALALLDATIRLLPGVMGA
AESAEEESFSDGTLLEYPHYTRPAVWQGRAVPEALLSGHHAAIAAWRRAEAEAVTRRRRPDLWARHIASQPALP
>Mature_233_residues
SFRATIITLFPEMFPGPLGQSLAGRALETGIWALDCVNLRDFGLGRHRAVDDTPFGGGAGMVLRPDVLDAAIGQARDGRP
LIALTPRGAPLTQARIRRLAEGPGAVLLCGRFEGFDQRALDANEAEEISLGDFVLSGGEIAALALLDATIRLLPGVMGAA
ESAEEESFSDGTLLEYPHYTRPAVWQGRAVPEALLSGHHAAIAAWRRAEAEAVTRRRRPDLWARHIASQPALP

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=220, Percent_Identity=45.9090909090909, Blast_Score=180, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25061; Mature: 24930

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRATIITLFPEMFPGPLGQSLAGRALETGIWALDCVNLRDFGLGRHRAVDDTPFGGGA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCCCCCCCCCC
GMVLRPDVLDAAIGQARDGRPLIALTPRGAPLTQARIRRLAEGPGAVLLCGRFEGFDQRA
CCEECCHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHH
LDANEAEEISLGDFVLSGGEIAALALLDATIRLLPGVMGAAESAEEESFSDGTLLEYPHY
CCCCCCCCEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEECCCC
TRPAVWQGRAVPEALLSGHHAAIAAWRRAEAEAVTRRRRPDLWARHIASQPALP
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SFRATIITLFPEMFPGPLGQSLAGRALETGIWALDCVNLRDFGLGRHRAVDDTPFGGGA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCCCCCCCCCC
GMVLRPDVLDAAIGQARDGRPLIALTPRGAPLTQARIRRLAEGPGAVLLCGRFEGFDQRA
CCEECCHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHH
LDANEAEEISLGDFVLSGGEIAALALLDATIRLLPGVMGAAESAEEESFSDGTLLEYPHY
CCCCCCCCEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEECCCC
TRPAVWQGRAVPEALLSGHHAAIAAWRRAEAEAVTRRRRPDLWARHIASQPALP
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA