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The map label for this gene is ispH2 [H]

Identifier: 148260826

GI number: 148260826

Start: 2021940

End: 2022923

Strand: Reverse

Name: ispH2 [H]

Synonym: Acry_1832

Alternate gene names: 148260826

Gene position: 2022923-2021940 (Counterclockwise)

Preceding gene: 148260833

Following gene: 148260825

Centisome position: 59.69

GC content: 62.5

Gene sequence:

>984_bases
ATGGATCTGCCTCATACCATGACCGAATGCGCAGATCCGGCCGTGGACCAGACGGTCATGAAAGTCGTGCTGGCGCAGCC
GCGCGGGTTCTGCGCCGGCGTCGAGCGGGCGATCGAGATCGTTGAACGGGCATTGGCGAAATTCGGTCCGCCGATCTATG
TGCGCCATGAAATCGTGCATAACCGCCATGTGGTGGAGGATTTGAGATCCCGCGGCGCGGTGTTCGTCGACGAGATTTCG
GAAGTGCCGGATGGGGCGATTACGGTGTTTTCTGCGCATGGTGTCGCGCGGAAAGTGCAGCAGAGCGCCGCGAACCGCGC
CCTGCCGGTGATCGATGCCACCTGCCCGCTGGTTGCCAAGGTCCATTCCGAAGGGCGGCGCTACGCTGGTCAGGGGCGGG
AGATCGTGCTGATCGGCCATGCCGGGCACGCGGAAGTCGAGGGGACGATCGGCCAGATCGACGGTCGGGTCTATCTTGTG
CAGACCGTCGAGGATGTGGCGTCACTGCAGGTGAGCGACCCGGACAAGCTTGCCTATATCACACAGACGACGCTTTCGGT
TGACGACACGCGCGGAATCATTGCGGCGCTGGTCGAGCGCTTTCCCGGGATCGTCGGTCCCGACGTGCGCGATATCTGCT
ATGCGACCCAAAACCGGCAGGAAGCCGTGCGGCATCTGGCCGAACAGGTCGACGTTATCCTGGTCGTCGGCAGCCGAAAT
TCATCGAATTCCAATCGTTTGTGCGAGATCGGCGCCGAATTGGGGCGTCCGTCCTATCTGATCGACGATGCCAGCCATCT
CGATCCGTCCTGGTTCAAGGGCATCCGCGCCGTCGGGGTTACGGCGGGCGCGTCGGCGCCGGAAGTGCTGGTCAAAGGGG
TGATCGATGGCCTGCGGAAGTTCGGCGCCATCGAAATCGATACCCTTGCCGGCACGCCGGAAGATGTCAGGTTCAAACTG
CCCACCGAAGTGAGCGATGCCTGA

Upstream 100 bases:

>100_bases
CTTGCGAAGCCGCCTTGGGATGACTTATTTCAGAGAAACGGCGTACTGCCGGAGTGGATTTCCCACAATCGCGAATTCCC
GATTCGAAAGGCGACAGACC

Downstream 100 bases:

>100_bases
ACGGCGCATCGGCAGGTTGGGAAATATCAGGAGTTACCAATGGGCGTGCCGCTGAAGCAGGCGATCAAGGTTGGAGCCTA
CGTGCTGAAACAGCATTTGA

Product: hydroxymethylbutenyl pyrophosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MDLPHTMTECADPAVDQTVMKVVLAQPRGFCAGVERAIEIVERALAKFGPPIYVRHEIVHNRHVVEDLRSRGAVFVDEIS
EVPDGAITVFSAHGVARKVQQSAANRALPVIDATCPLVAKVHSEGRRYAGQGREIVLIGHAGHAEVEGTIGQIDGRVYLV
QTVEDVASLQVSDPDKLAYITQTTLSVDDTRGIIAALVERFPGIVGPDVRDICYATQNRQEAVRHLAEQVDVILVVGSRN
SSNSNRLCEIGAELGRPSYLIDDASHLDPSWFKGIRAVGVTAGASAPEVLVKGVIDGLRKFGAIEIDTLAGTPEDVRFKL
PTEVSDA

Sequences:

>Translated_327_residues
MDLPHTMTECADPAVDQTVMKVVLAQPRGFCAGVERAIEIVERALAKFGPPIYVRHEIVHNRHVVEDLRSRGAVFVDEIS
EVPDGAITVFSAHGVARKVQQSAANRALPVIDATCPLVAKVHSEGRRYAGQGREIVLIGHAGHAEVEGTIGQIDGRVYLV
QTVEDVASLQVSDPDKLAYITQTTLSVDDTRGIIAALVERFPGIVGPDVRDICYATQNRQEAVRHLAEQVDVILVVGSRN
SSNSNRLCEIGAELGRPSYLIDDASHLDPSWFKGIRAVGVTAGASAPEVLVKGVIDGLRKFGAIEIDTLAGTPEDVRFKL
PTEVSDA
>Mature_327_residues
MDLPHTMTECADPAVDQTVMKVVLAQPRGFCAGVERAIEIVERALAKFGPPIYVRHEIVHNRHVVEDLRSRGAVFVDEIS
EVPDGAITVFSAHGVARKVQQSAANRALPVIDATCPLVAKVHSEGRRYAGQGREIVLIGHAGHAEVEGTIGQIDGRVYLV
QTVEDVASLQVSDPDKLAYITQTTLSVDDTRGIIAALVERFPGIVGPDVRDICYATQNRQEAVRHLAEQVDVILVVGSRN
SSNSNRLCEIGAELGRPSYLIDDASHLDPSWFKGIRAVGVTAGASAPEVLVKGVIDGLRKFGAIEIDTLAGTPEDVRFKL
PTEVSDA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=308, Percent_Identity=58.4415584415584, Blast_Score=368, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35189; Mature: 35189

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLPHTMTECADPAVDQTVMKVVLAQPRGFCAGVERAIEIVERALAKFGPPIYVRHEIVH
CCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
NRHVVEDLRSRGAVFVDEISEVPDGAITVFSAHGVARKVQQSAANRALPVIDATCPLVAK
HHHHHHHHHHCCCEEEHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEECCCHHHHH
VHSEGRRYAGQGREIVLIGHAGHAEVEGTIGQIDGRVYLVQTVEDVASLQVSDPDKLAYI
HHHHCHHHCCCCCEEEEEECCCCCCCCCCCEECCCEEEEEEEHHHHHHCCCCCCCCEEEE
TQTTLSVDDTRGIIAALVERFPGIVGPDVRDICYATQNRQEAVRHLAEQVDVILVVGSRN
EEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCC
SSNSNRLCEIGAELGRPSYLIDDASHLDPSWFKGIRAVGVTAGASAPEVLVKGVIDGLRK
CCCCCHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHH
FGAIEIDTLAGTPEDVRFKLPTEVSDA
CCCEEEEECCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MDLPHTMTECADPAVDQTVMKVVLAQPRGFCAGVERAIEIVERALAKFGPPIYVRHEIVH
CCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
NRHVVEDLRSRGAVFVDEISEVPDGAITVFSAHGVARKVQQSAANRALPVIDATCPLVAK
HHHHHHHHHHCCCEEEHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEECCCHHHHH
VHSEGRRYAGQGREIVLIGHAGHAEVEGTIGQIDGRVYLVQTVEDVASLQVSDPDKLAYI
HHHHCHHHCCCCCEEEEEECCCCCCCCCCCEECCCEEEEEEEHHHHHHCCCCCCCCEEEE
TQTTLSVDDTRGIIAALVERFPGIVGPDVRDICYATQNRQEAVRHLAEQVDVILVVGSRN
EEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCC
SSNSNRLCEIGAELGRPSYLIDDASHLDPSWFKGIRAVGVTAGASAPEVLVKGVIDGLRK
CCCCCHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHH
FGAIEIDTLAGTPEDVRFKLPTEVSDA
CCCEEEEECCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]