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The map label for this gene is pmbA [H]

Identifier: 148260780

GI number: 148260780

Start: 1967004

End: 1968332

Strand: Reverse

Name: pmbA [H]

Synonym: Acry_1785

Alternate gene names: 148260780

Gene position: 1968332-1967004 (Counterclockwise)

Preceding gene: 148260781

Following gene: 148260774

Centisome position: 58.08

GC content: 73.66

Gene sequence:

>1329_bases
GTGAACCGGCTCGACCTGCTCGACGCCCTGCTCGACGCCGCGAAGCGCGCCGGCGCGGAGTCCGCCGATGCGCTGATGGG
CTCCGGCACCTCGCTCTCCGTCGGCCGCCGGCTCGGCAAGGTCGAGGAGATCGAGCGCTCCGAAAGCGACACGGTCGGCC
TGCGCGTCTTCGTGGGAAGCCGCAGCGCCATCGTCTCCTCCGGCGCGATCGACCCCGCAGGCTTCGCCCGCCTCGCCGAA
CAGGCGGTGGCGATGGCCCGCGTCGTCCCCGAGGATCGCTTCTCCACCATCCCCGACGCGCCGACCCCGCCCGATTCCGC
CCCGCTCGACATGGACGATCCGGCCGAGCCCGGCGTCGAGGCGCTGCTCGCCCGCGCTGCCGCCGCGGAAGAGGCGGCCC
TCGCGGTCAAGGGCATCACCAATTCCGAGGGCGCCGGCGCCTCCTGGTCGCGCAGCCACATCGCGCTGGCCACCTCGCGC
GGCTTCGCCGGCGCCTATTCCCGCTCCAGTCACGGCGTCTCCGTCTCGCCCATCGCCGGCGAAGGGGTGGGGATGCAGCG
CGACTATGAATATTCCGCCGCCGTCCACGGCGCCGACCTCGCCGACCCCGCCGGCCTCGGCCGCGCCGCCGCCGAGCGCG
CGGTCAGCCGGCTCGACCCGCGCCGCCCGGCCACCGCGAAACTCCCGGTGGTCTACCACCCCCGCGTCGCCGGCTCGCTG
CTCGGCCATTTCGCCGGCGCCATCAACGGCGCCGCCATCGCCCGCGGCACCAGCTTCCTCAAGGATGCGATGGGTCAGGC
GATCTTCGCGCCCGGCATCTCCATCATCGACGATCCGCTGCGCCGCCGCGGCCTGCGCTCGCGCAGCTTCGACGGCGAGG
GCCAGACCGTCTCCCGCCGCGCCCTGATCGAGGATGGCCGGCTGACCACCTGGCTGCTCGATTCCCGCAGCGCCGCCCAG
CTCGGCCTCGCCACCACCGGCCACGCCGCCCGCGGCACCTCCGGCCCGCCATCCCCGGCGCCGAGCAATCTCTACATGCA
GCCCGGCGCGCTCTCGCCCGAGGCGCTGATCGCCGATATCGGCCTCGGCCTCTACGTCATCGAGCTGATCGGCATGGGCG
TGAACGGCATCACCGGCGATTATTCGCGCGGCGCCGCCGGCTTCATGATCCGCGACGGCCAGCTCGCCGAGCCGGTCGCC
GAGGTCACCGTCGCCGGCAATCTGCGCGAGATGTTCCTCCACCTCACCCCGGCGAACGACCTCGAATTCCGCCGCGGCAC
CGACGCGCCGACGCTGCGGATCGAGGGCATGACGATGGCCGGAGCCTGA

Upstream 100 bases:

>100_bases
ACGTCGCCACCCGCATCTTCCGCGCCAAGCCCACCCTTGCCGCGATCGGCCCGGTCGGCCGCGTGCCGCAGATGCCGAAA
ATCATCGAGAGGCTGGCGGC

Downstream 100 bases:

>100_bases
GCCGAGGGCTCAGGACGGCCGGAGCCTGAGCCGGGAGGCCCAGAACGGCCGGAGCCTGAGCCGAGGGCTCAAGATGGCGG
GCGGCTGAGCGAGCCTCAGC

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MNRLDLLDALLDAAKRAGAESADALMGSGTSLSVGRRLGKVEEIERSESDTVGLRVFVGSRSAIVSSGAIDPAGFARLAE
QAVAMARVVPEDRFSTIPDAPTPPDSAPLDMDDPAEPGVEALLARAAAAEEAALAVKGITNSEGAGASWSRSHIALATSR
GFAGAYSRSSHGVSVSPIAGEGVGMQRDYEYSAAVHGADLADPAGLGRAAAERAVSRLDPRRPATAKLPVVYHPRVAGSL
LGHFAGAINGAAIARGTSFLKDAMGQAIFAPGISIIDDPLRRRGLRSRSFDGEGQTVSRRALIEDGRLTTWLLDSRSAAQ
LGLATTGHAARGTSGPPSPAPSNLYMQPGALSPEALIADIGLGLYVIELIGMGVNGITGDYSRGAAGFMIRDGQLAEPVA
EVTVAGNLREMFLHLTPANDLEFRRGTDAPTLRIEGMTMAGA

Sequences:

>Translated_442_residues
MNRLDLLDALLDAAKRAGAESADALMGSGTSLSVGRRLGKVEEIERSESDTVGLRVFVGSRSAIVSSGAIDPAGFARLAE
QAVAMARVVPEDRFSTIPDAPTPPDSAPLDMDDPAEPGVEALLARAAAAEEAALAVKGITNSEGAGASWSRSHIALATSR
GFAGAYSRSSHGVSVSPIAGEGVGMQRDYEYSAAVHGADLADPAGLGRAAAERAVSRLDPRRPATAKLPVVYHPRVAGSL
LGHFAGAINGAAIARGTSFLKDAMGQAIFAPGISIIDDPLRRRGLRSRSFDGEGQTVSRRALIEDGRLTTWLLDSRSAAQ
LGLATTGHAARGTSGPPSPAPSNLYMQPGALSPEALIADIGLGLYVIELIGMGVNGITGDYSRGAAGFMIRDGQLAEPVA
EVTVAGNLREMFLHLTPANDLEFRRGTDAPTLRIEGMTMAGA
>Mature_442_residues
MNRLDLLDALLDAAKRAGAESADALMGSGTSLSVGRRLGKVEEIERSESDTVGLRVFVGSRSAIVSSGAIDPAGFARLAE
QAVAMARVVPEDRFSTIPDAPTPPDSAPLDMDDPAEPGVEALLARAAAAEEAALAVKGITNSEGAGASWSRSHIALATSR
GFAGAYSRSSHGVSVSPIAGEGVGMQRDYEYSAAVHGADLADPAGLGRAAAERAVSRLDPRRPATAKLPVVYHPRVAGSL
LGHFAGAINGAAIARGTSFLKDAMGQAIFAPGISIIDDPLRRRGLRSRSFDGEGQTVSRRALIEDGRLTTWLLDSRSAAQ
LGLATTGHAARGTSGPPSPAPSNLYMQPGALSPEALIADIGLGLYVIELIGMGVNGITGDYSRGAAGFMIRDGQLAEPVA
EVTVAGNLREMFLHLTPANDLEFRRGTDAPTLRIEGMTMAGA

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=434, Percent_Identity=37.5576036866359, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789640, Length=234, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 45639; Mature: 45639

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLDLLDALLDAAKRAGAESADALMGSGTSLSVGRRLGKVEEIERSESDTVGLRVFVGS
CCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHCHHHHHHCCCCCCEEEEEEECC
RSAIVSSGAIDPAGFARLAEQAVAMARVVPEDRFSTIPDAPTPPDSAPLDMDDPAEPGVE
CHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
ALLARAAAAEEAALAVKGITNSEGAGASWSRSHIALATSRGFAGAYSRSSHGVSVSPIAG
HHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCC
EGVGMQRDYEYSAAVHGADLADPAGLGRAAAERAVSRLDPRRPATAKLPVVYHPRVAGSL
CCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHH
LGHFAGAINGAAIARGTSFLKDAMGQAIFAPGISIIDDPLRRRGLRSRSFDGEGQTVSRR
HHHHHHHHCCHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHH
ALIEDGRLTTWLLDSRSAAQLGLATTGHAARGTSGPPSPAPSNLYMQPGALSPEALIADI
HHHCCCCEEEEEECCCCHHHHCCEECCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHH
GLGLYVIELIGMGVNGITGDYSRGAAGFMIRDGQLAEPVAEVTVAGNLREMFLHLTPAND
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHCHHHHHHHCCCHHHEEEEECCCCC
LEFRRGTDAPTLRIEGMTMAGA
CCCCCCCCCCEEEECCEEECCC
>Mature Secondary Structure
MNRLDLLDALLDAAKRAGAESADALMGSGTSLSVGRRLGKVEEIERSESDTVGLRVFVGS
CCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHCHHHHHHCCCCCCEEEEEEECC
RSAIVSSGAIDPAGFARLAEQAVAMARVVPEDRFSTIPDAPTPPDSAPLDMDDPAEPGVE
CHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
ALLARAAAAEEAALAVKGITNSEGAGASWSRSHIALATSRGFAGAYSRSSHGVSVSPIAG
HHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCC
EGVGMQRDYEYSAAVHGADLADPAGLGRAAAERAVSRLDPRRPATAKLPVVYHPRVAGSL
CCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHH
LGHFAGAINGAAIARGTSFLKDAMGQAIFAPGISIIDDPLRRRGLRSRSFDGEGQTVSRR
HHHHHHHHCCHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHH
ALIEDGRLTTWLLDSRSAAQLGLATTGHAARGTSGPPSPAPSNLYMQPGALSPEALIADI
HHHCCCCEEEEEECCCCHHHHCCEECCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHH
GLGLYVIELIGMGVNGITGDYSRGAAGFMIRDGQLAEPVAEVTVAGNLREMFLHLTPAND
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHCHHHHHHHCCCHHHEEEEECCCCC
LEFRRGTDAPTLRIEGMTMAGA
CCCCCCCCCCEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]