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The map label for this gene is gutB [H]

Identifier: 148260656

GI number: 148260656

Start: 1830012

End: 1831031

Strand: Reverse

Name: gutB [H]

Synonym: Acry_1659

Alternate gene names: 148260656

Gene position: 1831031-1830012 (Counterclockwise)

Preceding gene: 148260657

Following gene: 148260655

Centisome position: 54.03

GC content: 69.12

Gene sequence:

>1020_bases
ATGAAGGCTCTCTACATCCGCGGCGTGCGGGACATCACGATCGGCGATGCGCCCGAACCCGTGCCGGGAAACGACCGCAT
TGCCGTCGAGGTCGCCGGCGTCGGTGTCTGCGGCAGCGACCTGCATTATTACCTCGAAGGATCGATCGGCAGCCAGGTCA
TCGTCGAGCCTTTCGTGCCGGGCCATGAATTCGCCGCCCGCGTGATCGAGGCGCGTCCGGAACTCGGCCTTGCCAAGGGC
GAACTCATCGCGGTCGACCCCGCCATGCCCTGCGGCCATTGCGAATGGTGCCATCGCGGCGAGATCAACCTCTGCCCCGA
CACCGTGTTCAACGGCGCGCCGCCCTATCCTGGGGCGATGGCCGAGCGCATGGCGATCGAGCCGCGCCAGGTCGTGAAGG
TGCCGCAGCATTTCACGATCGAGGAGACGATGATGCTGGAGCCGCTCGGCGTCGCGATCCACGCCATCGATCTCGCGAGG
CCGCGCCTGCTGGAATCGGTTGCGGTGATCGGTTGCGGGCCGATCGGCCTGCTGATGATCGCGCTCGCCCGCCTCGCCGG
CGTCGGGCAGATCCTGGCGATCGATCCGGTAGACTATCGCCGCGCGCATGCTGGCGCGCTGGGTGCCGACCGCGTCAGCG
CCGACCGGCACGACATCGCCGCCTGGACCGATGGCCGCGGCGTCGACCTCGTGCTCGAGGCGACCAACGCGCCGGAGGGA
TTCCAGCACGCGGCCGATGTCGCGCGGATCGGCGGGCGGATCGTCCTCGTCGGCATTCCCGAAGGGGATTCCTACACGCT
TGCCGCCGGGGCGGCGCGGCGCAAGGGGCTTTCCGTCAAGTTCTCGCGCCGCATGGGCCATGTCTATCCGCGCGCCATCC
GCCTCGTCGCCGAACGGCGGGTCGATGTCGCGCGCATCGTCACGCACCGGTTTCCGCTGGCTGAGGGCGCCCGCGCCTTC
GAGATTCAGGCCGCCTGCGCCGATGGGGCGCTCAAGAGCCTCATCCTGCCGAACGCCTGA

Upstream 100 bases:

>100_bases
CCGCGCCGCCCTCTATGCCCGCCTGCGCCGCGCCCATGCCGGGCTCTATCCGGCGCTGCGGCCGTTCGGCGCCGCCCCGT
TGACCTGAAAGGATACCACG

Downstream 100 bases:

>100_bases
ACGGCCGGATAATTCCGGTTGCAGGCGATGATTAATCCGGGCATACGTGAAAAAATAATCAAACTTCGCGGCAACCCCGC
CTTCAAGGAGGATACGTCAT

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKALYIRGVRDITIGDAPEPVPGNDRIAVEVAGVGVCGSDLHYYLEGSIGSQVIVEPFVPGHEFAARVIEARPELGLAKG
ELIAVDPAMPCGHCEWCHRGEINLCPDTVFNGAPPYPGAMAERMAIEPRQVVKVPQHFTIEETMMLEPLGVAIHAIDLAR
PRLLESVAVIGCGPIGLLMIALARLAGVGQILAIDPVDYRRAHAGALGADRVSADRHDIAAWTDGRGVDLVLEATNAPEG
FQHAADVARIGGRIVLVGIPEGDSYTLAAGAARRKGLSVKFSRRMGHVYPRAIRLVAERRVDVARIVTHRFPLAEGARAF
EIQAACADGALKSLILPNA

Sequences:

>Translated_339_residues
MKALYIRGVRDITIGDAPEPVPGNDRIAVEVAGVGVCGSDLHYYLEGSIGSQVIVEPFVPGHEFAARVIEARPELGLAKG
ELIAVDPAMPCGHCEWCHRGEINLCPDTVFNGAPPYPGAMAERMAIEPRQVVKVPQHFTIEETMMLEPLGVAIHAIDLAR
PRLLESVAVIGCGPIGLLMIALARLAGVGQILAIDPVDYRRAHAGALGADRVSADRHDIAAWTDGRGVDLVLEATNAPEG
FQHAADVARIGGRIVLVGIPEGDSYTLAAGAARRKGLSVKFSRRMGHVYPRAIRLVAERRVDVARIVTHRFPLAEGARAF
EIQAACADGALKSLILPNA
>Mature_339_residues
MKALYIRGVRDITIGDAPEPVPGNDRIAVEVAGVGVCGSDLHYYLEGSIGSQVIVEPFVPGHEFAARVIEARPELGLAKG
ELIAVDPAMPCGHCEWCHRGEINLCPDTVFNGAPPYPGAMAERMAIEPRQVVKVPQHFTIEETMMLEPLGVAIHAIDLAR
PRLLESVAVIGCGPIGLLMIALARLAGVGQILAIDPVDYRRAHAGALGADRVSADRHDIAAWTDGRGVDLVLEATNAPEG
FQHAADVARIGGRIVLVGIPEGDSYTLAAGAARRKGLSVKFSRRMGHVYPRAIRLVAERRVDVARIVTHRFPLAEGARAF
EIQAACADGALKSLILPNA

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=328, Percent_Identity=32.0121951219512, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1788073, Length=319, Percent_Identity=30.0940438871473, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1790045, Length=340, Percent_Identity=28.2352941176471, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790718, Length=303, Percent_Identity=29.042904290429, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787863, Length=329, Percent_Identity=27.355623100304, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786825, Length=393, Percent_Identity=28.7531806615776, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788407, Length=318, Percent_Identity=28.3018867924528, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI226510992, Length=329, Percent_Identity=25.531914893617, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788075, Length=335, Percent_Identity=24.4776119402985, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082125, Length=347, Percent_Identity=22.478386167147, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786518, Length=254, Percent_Identity=28.3464566929134, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081918, Length=232, Percent_Identity=30.6034482758621, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=325, Percent_Identity=32, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17562878, Length=331, Percent_Identity=31.7220543806647, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319955, Length=350, Percent_Identity=30.2857142857143, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322619, Length=350, Percent_Identity=30.2857142857143, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323099, Length=322, Percent_Identity=27.639751552795, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6319258, Length=276, Percent_Identity=27.1739130434783, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319257, Length=248, Percent_Identity=27.8225806451613, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17137530, Length=339, Percent_Identity=32.7433628318584, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI17737897, Length=343, Percent_Identity=31.7784256559767, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 36129; Mature: 36129

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALYIRGVRDITIGDAPEPVPGNDRIAVEVAGVGVCGSDLHYYLEGSIGSQVIVEPFVP
CCEEEEECCEEEECCCCCCCCCCCCEEEEEEEECEEECCCEEEEEECCCCCEEEEECCCC
GHEFAARVIEARPELGLAKGELIAVDPAMPCGHCEWCHRGEINLCPDTVFNGAPPYPGAM
CHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEECCHHHCCCCCCCCCHH
AERMAIEPRQVVKVPQHFTIEETMMLEPLGVAIHAIDLARPRLLESVAVIGCGPIGLLMI
HHHHCCCCHHHHCCCCCCEECHHHEECCCCCEEEEEHHHCHHHHHHHHHCCCCHHHHHHH
ALARLAGVGQILAIDPVDYRRAHAGALGADRVSADRHDIAAWTDGRGVDLVLEATNAPEG
HHHHHCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCH
FQHAADVARIGGRIVLVGIPEGDSYTLAAGAARRKGLSVKFSRRMGHVYPRAIRLVAERR
HHHHHHHHHHCCEEEEEEECCCCCEEEECCHHHHCCCCEEEHHHCCCHHHHHHHHHHHHC
VDVARIVTHRFPLAEGARAFEIQAACADGALKSLILPNA
HHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKALYIRGVRDITIGDAPEPVPGNDRIAVEVAGVGVCGSDLHYYLEGSIGSQVIVEPFVP
CCEEEEECCEEEECCCCCCCCCCCCEEEEEEEECEEECCCEEEEEECCCCCEEEEECCCC
GHEFAARVIEARPELGLAKGELIAVDPAMPCGHCEWCHRGEINLCPDTVFNGAPPYPGAM
CHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEECCHHHCCCCCCCCCHH
AERMAIEPRQVVKVPQHFTIEETMMLEPLGVAIHAIDLARPRLLESVAVIGCGPIGLLMI
HHHHCCCCHHHHCCCCCCEECHHHEECCCCCEEEEEHHHCHHHHHHHHHCCCCHHHHHHH
ALARLAGVGQILAIDPVDYRRAHAGALGADRVSADRHDIAAWTDGRGVDLVLEATNAPEG
HHHHHCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCH
FQHAADVARIGGRIVLVGIPEGDSYTLAAGAARRKGLSVKFSRRMGHVYPRAIRLVAERR
HHHHHHHHHHCCEEEEEEECCCCCEEEECCHHHHCCCCEEEHHHCCCHHHHHHHHHHHHC
VDVARIVTHRFPLAEGARAFEIQAACADGALKSLILPNA
HHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]