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The map label for this gene is fliF [H]

Identifier: 148260475

GI number: 148260475

Start: 1638504

End: 1640066

Strand: Reverse

Name: fliF [H]

Synonym: Acry_1475

Alternate gene names: 148260475

Gene position: 1640066-1638504 (Counterclockwise)

Preceding gene: 148260476

Following gene: 148260474

Centisome position: 48.39

GC content: 59.63

Gene sequence:

>1563_bases
ATGAAGCAGTTGATCGCGAACCTGCGGGCGCTCGGCCCGGTGAAGCTTGGCGCCATGGGCGCCGTGGCTGTTGCCGTGCT
CGGGCTGATGGCGGCACTGGTGTTGACGGGGGGGCCATCCCCGTCCGCCACGCTCTATTCCGGCCTTGGTCTCAAGGACG
CCAGCCACATTACCGAGACGCTGAAAGCGGCACATATTCGTTATGCGATCGGCAACGGCGGTCATAGCGTTCTCGTCACC
CCTGCCCAACTCGACGAGGCGCGGCTGTTGCTGGCCCAGCACAATCTTCCAGCCGGCTCGTCCAACGGCTTCGCGATATT
CGATCACAAGAATCCCCTATTAGGATCCAGTTTTCTCAACCGGATCGATGAGACGCGCGCACTCGATTCCGAGCTTGAGC
GGACGATTAATCTTATCCACGGGGTCGAAGCGTCGCGCGTTCAGGTTGTGCTGCCGCGACGCGACGAATTCAGTTTGAAT
ACTGCCCCGGCCCAGGCGAGCGTCATGTTGTCGCTCGCTGGCGTCGGCCCTCTCGACCACGAAAGCGTTGAGGCCATCCT
CAACTTGGTCGCATCCGCAGTGCCGGGGCTCAAGCCGAACAACATTTCGATTGTCGACAATCGCGGCGATCTCCTTGCTC
AGGCCGGTCGGACCGACAATTCTCTTCTCAGTTCACGTGATGCGGCGTTGAAGCAATCAACGGAGCGGCAACTGTCGGAA
GCGGTGCGCTCCATGCTTGCTGCCGTGGTCGGTCCGAACCAGGTCAGGGTTGTTACGGCCGTTTCCATGGACTTCGACCG
GAGCGAACAAACCTCGACAACCTATAACCCGAACGGACAGGTTGTACGTAGTCAGCAACAGACAAGGTCCAAAAGTTCAC
GCAGCAGCGGGACGAACAAGACCGTATCCGTATCAAACAATCTCCCAGGTGCGACGTCCAGCAAAGCAGGGCCGAAACGG
CTCGATACTCAGAGCAAGTCCACTCAGACGACCAATTACGAGATCAGCCAGGACGTCAAGCACATTGTTCATGCGACGCC
ACAGATCACGCGTATCAGCGTCGCGGTGATGCTGGACGACGTGAAGACGACAAACCCGAAAGGCAAGGTCATCTGGACGC
CGCGCTCCCCGGCGCAGGTCGCGCAAATTCGTAAGCTGGTTGAAACGGCCATCGGATATGACAAATCCCGCGGCGACGTC
GTCGACGTTCAGTCACTCCAGTTTGCACCCAATGAACTACCGGTTAGCGCCAAGCGGTCTCTGATCGGACGCTTCCTGTC
GAGTGGTCTGCTGATGCCGTTGCTACGCCTCCTCGTCACCGCAGCCATCGGGATTGCCGCGCTGCTTGTGGTCTTCCGGC
CGATGGTTCGTCGTATCATTACGCCACCCGAAAATACGACCGCAGAACCTGCGCTCCTGGCCGAACAGAACGGGGCCATC
GCCGGCCCGGCAACGACGGAGGAGACCAATCCTGTCAAAGTGATCGCTGATCTGATCGACCGTCATCCTGAAGCCAGCGT
CGCGATCCTGCGCGACTGGCTAGGCCAAGAAGGCGCCCGATGA

Upstream 100 bases:

>100_bases
GGCGATTCTTGTCCTGCGACAAAAGGTCGCAATCGCAGGATGGATTCATGCAGTTCTCCACACGGCTGCGTTGGGACTCA
AAATTGGGGTCCCTCGGCTG

Downstream 100 bases:

>100_bases
GCGATACGTCAGATCGCAATCTCAACGGGGCCTCGCGCGCGGCTGCCTTGCTCCTTGCTCTTGGTGAAGAGGCTGGCGGC
AAACTGATGGCGATGCTCAA

Product: flagellar M-ring protein FliF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKQLIANLRALGPVKLGAMGAVAVAVLGLMAALVLTGGPSPSATLYSGLGLKDASHITETLKAAHIRYAIGNGGHSVLVT
PAQLDEARLLLAQHNLPAGSSNGFAIFDHKNPLLGSSFLNRIDETRALDSELERTINLIHGVEASRVQVVLPRRDEFSLN
TAPAQASVMLSLAGVGPLDHESVEAILNLVASAVPGLKPNNISIVDNRGDLLAQAGRTDNSLLSSRDAALKQSTERQLSE
AVRSMLAAVVGPNQVRVVTAVSMDFDRSEQTSTTYNPNGQVVRSQQQTRSKSSRSSGTNKTVSVSNNLPGATSSKAGPKR
LDTQSKSTQTTNYEISQDVKHIVHATPQITRISVAVMLDDVKTTNPKGKVIWTPRSPAQVAQIRKLVETAIGYDKSRGDV
VDVQSLQFAPNELPVSAKRSLIGRFLSSGLLMPLLRLLVTAAIGIAALLVVFRPMVRRIITPPENTTAEPALLAEQNGAI
AGPATTEETNPVKVIADLIDRHPEASVAILRDWLGQEGAR

Sequences:

>Translated_520_residues
MKQLIANLRALGPVKLGAMGAVAVAVLGLMAALVLTGGPSPSATLYSGLGLKDASHITETLKAAHIRYAIGNGGHSVLVT
PAQLDEARLLLAQHNLPAGSSNGFAIFDHKNPLLGSSFLNRIDETRALDSELERTINLIHGVEASRVQVVLPRRDEFSLN
TAPAQASVMLSLAGVGPLDHESVEAILNLVASAVPGLKPNNISIVDNRGDLLAQAGRTDNSLLSSRDAALKQSTERQLSE
AVRSMLAAVVGPNQVRVVTAVSMDFDRSEQTSTTYNPNGQVVRSQQQTRSKSSRSSGTNKTVSVSNNLPGATSSKAGPKR
LDTQSKSTQTTNYEISQDVKHIVHATPQITRISVAVMLDDVKTTNPKGKVIWTPRSPAQVAQIRKLVETAIGYDKSRGDV
VDVQSLQFAPNELPVSAKRSLIGRFLSSGLLMPLLRLLVTAAIGIAALLVVFRPMVRRIITPPENTTAEPALLAEQNGAI
AGPATTEETNPVKVIADLIDRHPEASVAILRDWLGQEGAR
>Mature_520_residues
MKQLIANLRALGPVKLGAMGAVAVAVLGLMAALVLTGGPSPSATLYSGLGLKDASHITETLKAAHIRYAIGNGGHSVLVT
PAQLDEARLLLAQHNLPAGSSNGFAIFDHKNPLLGSSFLNRIDETRALDSELERTINLIHGVEASRVQVVLPRRDEFSLN
TAPAQASVMLSLAGVGPLDHESVEAILNLVASAVPGLKPNNISIVDNRGDLLAQAGRTDNSLLSSRDAALKQSTERQLSE
AVRSMLAAVVGPNQVRVVTAVSMDFDRSEQTSTTYNPNGQVVRSQQQTRSKSSRSSGTNKTVSVSNNLPGATSSKAGPKR
LDTQSKSTQTTNYEISQDVKHIVHATPQITRISVAVMLDDVKTTNPKGKVIWTPRSPAQVAQIRKLVETAIGYDKSRGDV
VDVQSLQFAPNELPVSAKRSLIGRFLSSGLLMPLLRLLVTAAIGIAALLVVFRPMVRRIITPPENTTAEPALLAEQNGAI
AGPATTEETNPVKVIADLIDRHPEASVAILRDWLGQEGAR

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=460, Percent_Identity=30.2173913043478, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 55228; Mature: 55228

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLIANLRALGPVKLGAMGAVAVAVLGLMAALVLTGGPSPSATLYSGLGLKDASHITET
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHH
LKAAHIRYAIGNGGHSVLVTPAQLDEARLLLAQHNLPAGSSNGFAIFDHKNPLLGSSFLN
HHHHHHEEEECCCCCEEEEECCHHCHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHH
RIDETRALDSELERTINLIHGVEASRVQVVLPRRDEFSLNTAPAQASVMLSLAGVGPLDH
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHEEEEECCCCCCCH
ESVEAILNLVASAVPGLKPNNISIVDNRGDLLAQAGRTDNSLLSSRDAALKQSTERQLSE
HHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AVRSMLAAVVGPNQVRVVTAVSMDFDRSEQTSTTYNPNGQVVRSQQQTRSKSSRSSGTNK
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCEECCCCCEECCHHHHHHHHHCCCCCCC
TVSVSNNLPGATSSKAGPKRLDTQSKSTQTTNYEISQDVKHIVHATPQITRISVAVMLDD
EEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHEEEEEEEEEC
VKTTNPKGKVIWTPRSPAQVAQIRKLVETAIGYDKSRGDVVDVQSLQFAPNELPVSAKRS
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCCCHHHHHH
LIGRFLSSGLLMPLLRLLVTAAIGIAALLVVFRPMVRRIITPPENTTAEPALLAEQNGAI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCE
AGPATTEETNPVKVIADLIDRHPEASVAILRDWLGQEGAR
ECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKQLIANLRALGPVKLGAMGAVAVAVLGLMAALVLTGGPSPSATLYSGLGLKDASHITET
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHH
LKAAHIRYAIGNGGHSVLVTPAQLDEARLLLAQHNLPAGSSNGFAIFDHKNPLLGSSFLN
HHHHHHEEEECCCCCEEEEECCHHCHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHH
RIDETRALDSELERTINLIHGVEASRVQVVLPRRDEFSLNTAPAQASVMLSLAGVGPLDH
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHEEEEECCCCCCCH
ESVEAILNLVASAVPGLKPNNISIVDNRGDLLAQAGRTDNSLLSSRDAALKQSTERQLSE
HHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AVRSMLAAVVGPNQVRVVTAVSMDFDRSEQTSTTYNPNGQVVRSQQQTRSKSSRSSGTNK
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCEECCCCCEECCHHHHHHHHHCCCCCCC
TVSVSNNLPGATSSKAGPKRLDTQSKSTQTTNYEISQDVKHIVHATPQITRISVAVMLDD
EEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHEEEEEEEEEC
VKTTNPKGKVIWTPRSPAQVAQIRKLVETAIGYDKSRGDVVDVQSLQFAPNELPVSAKRS
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCCCHHHHHH
LIGRFLSSGLLMPLLRLLVTAAIGIAALLVVFRPMVRRIITPPENTTAEPALLAEQNGAI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCE
AGPATTEETNPVKVIADLIDRHPEASVAILRDWLGQEGAR
ECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002583; 11259647 [H]