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The map label for this gene is flbD [H]

Identifier: 148260472

GI number: 148260472

Start: 1635771

End: 1637126

Strand: Reverse

Name: flbD [H]

Synonym: Acry_1472

Alternate gene names: 148260472

Gene position: 1637126-1635771 (Counterclockwise)

Preceding gene: 148260473

Following gene: 148260471

Centisome position: 48.3

GC content: 57.96

Gene sequence:

>1356_bases
ATGCGGGTTCTCATCATCGGTTCACTGTCGGGAGAACTTGGTGTCGCAGCTCGTATTGCTGCGGCTCGGGGTGCAACGCT
CGCACAGGCTGAGACCGTTGATGCGGCCCTGGAATGTCTACGTGCCGATGCGAGGACCGACCTCATCATTTGCGACGTCA
CGCACGATTTCGGTGCCCTGATGAGGCGACTGGCCGCGGAGCGCTTCACAACGGCTGTCGTTGCCGCGGGAGCCGAAAAT
GATGAGAAGGCCGCCGTTGACGCAATTCGTGCTGGCGCACGTGAATATCTCCCTTTGCCTCCGGATTCCGACCTCATCGC
AGCCATTCTGTCGGCTGTTTCTACAGAGCAGAAGGGCGAGTTCATCGCGCGTGACCCCGCTATGCGGGCAGTACTGTCAC
AAGCTGACCAAGTAGCGCCATCCACTGCGTCAATCCTGATCACAGGAGAAAGCGGCACAGGGAAGGAGGTTCTTGCACGG
TATATTCACGAGCGCTCAAGGCGCCGCAAAGGGCCATTCGTCGCTCTCAACTGCGCAGCAATACCAGACACACTGCTCGA
GTCCGAGTTATTCGGACACGAGAAGGGGGCATTTTCCGGTGCAATCGCGCGCCGTACCGGGAAGTTTGAGGCTGCCGACC
GAGGCACTCTATTGCTTGATGAAATCGGAGAGATGGACGCGCGCTTGCAGGCAAAGCTGCTGCGCACGCTTCAGGAGCGC
ACTGTGGATCGAATTGGCGGTGATCGGCCCGTGCCAGTTGATGTGCGCATCCTTGCTGCCACGAACGTTGATCTGCGGTC
GGCCATTGCGGATGGCAGATTTCGGGAAGATCTGTTCTTTCGTCTGAATGTCATGACGTTGCGAATTCCGCCGTTGCGTC
AGCGTCCTGGGGATATTGCGCCCCTCGCGCACCATTTCGTGGCTCATTATGCCAGGCTGAATGAAGTTGGCACCAAGCAT
CTTACAAGCCGAGCTGTTGAGCGTCTGCTTGCCTATTCCTGGCGTGGAAATGTTCGAGAACTCGAAAATACCATGCATAG
GGCTGTGTTGTTGTCGTCAGGGCCGATGATCGACGTTGAGCATATCGGCATACCCACGGAAGCAGAGGAACCGTCATACC
TGCTCTCGCCCCACGCACATGCTACTGCCGGCGACACGATTGGCTCGCTGGTTGGCCAACGTGTCGAAGACGTCGAGCAG
GCACTGATTCTGGAGACGCTGAATCACTGTCTTGGCAACAGGACACATGCCGCCACGATCCTCGGAATTTCCATACGGGC
ACTGCGTAACAAGTTGCGTGAATATGCCGCTCGTGGTGCAGAAGTGCCGGCGCCACACAACGGTGTGGCCGCCTGA

Upstream 100 bases:

>100_bases
TCGCCTGATTGCACGTGGCGAAGTGGTGATGCTCGACGAAACGAAGCTCGGCATTACCATGACCGAAATCGTCAAATCTG
ACCGCAACGGTTGACCGACT

Downstream 100 bases:

>100_bases
TGGCTGCAACAGACCTCGCGGCCCTTCGCGGGCGACTGCAAGAATTCCGGATCGGTACCGATATCGGTCTGGCGATCGGC
GTGGTGTGCATCATCACGAT

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MRVLIIGSLSGELGVAARIAAARGATLAQAETVDAALECLRADARTDLIICDVTHDFGALMRRLAAERFTTAVVAAGAEN
DEKAAVDAIRAGAREYLPLPPDSDLIAAILSAVSTEQKGEFIARDPAMRAVLSQADQVAPSTASILITGESGTGKEVLAR
YIHERSRRRKGPFVALNCAAIPDTLLESELFGHEKGAFSGAIARRTGKFEAADRGTLLLDEIGEMDARLQAKLLRTLQER
TVDRIGGDRPVPVDVRILAATNVDLRSAIADGRFREDLFFRLNVMTLRIPPLRQRPGDIAPLAHHFVAHYARLNEVGTKH
LTSRAVERLLAYSWRGNVRELENTMHRAVLLSSGPMIDVEHIGIPTEAEEPSYLLSPHAHATAGDTIGSLVGQRVEDVEQ
ALILETLNHCLGNRTHAATILGISIRALRNKLREYAARGAEVPAPHNGVAA

Sequences:

>Translated_451_residues
MRVLIIGSLSGELGVAARIAAARGATLAQAETVDAALECLRADARTDLIICDVTHDFGALMRRLAAERFTTAVVAAGAEN
DEKAAVDAIRAGAREYLPLPPDSDLIAAILSAVSTEQKGEFIARDPAMRAVLSQADQVAPSTASILITGESGTGKEVLAR
YIHERSRRRKGPFVALNCAAIPDTLLESELFGHEKGAFSGAIARRTGKFEAADRGTLLLDEIGEMDARLQAKLLRTLQER
TVDRIGGDRPVPVDVRILAATNVDLRSAIADGRFREDLFFRLNVMTLRIPPLRQRPGDIAPLAHHFVAHYARLNEVGTKH
LTSRAVERLLAYSWRGNVRELENTMHRAVLLSSGPMIDVEHIGIPTEAEEPSYLLSPHAHATAGDTIGSLVGQRVEDVEQ
ALILETLNHCLGNRTHAATILGISIRALRNKLREYAARGAEVPAPHNGVAA
>Mature_451_residues
MRVLIIGSLSGELGVAARIAAARGATLAQAETVDAALECLRADARTDLIICDVTHDFGALMRRLAAERFTTAVVAAGAEN
DEKAAVDAIRAGAREYLPLPPDSDLIAAILSAVSTEQKGEFIARDPAMRAVLSQADQVAPSTASILITGESGTGKEVLAR
YIHERSRRRKGPFVALNCAAIPDTLLESELFGHEKGAFSGAIARRTGKFEAADRGTLLLDEIGEMDARLQAKLLRTLQER
TVDRIGGDRPVPVDVRILAATNVDLRSAIADGRFREDLFFRLNVMTLRIPPLRQRPGDIAPLAHHFVAHYARLNEVGTKH
LTSRAVERLLAYSWRGNVRELENTMHRAVLLSSGPMIDVEHIGIPTEAEEPSYLLSPHAHATAGDTIGSLVGQRVEDVEQ
ALILETLNHCLGNRTHAATILGISIRALRNKLREYAARGAEVPAPHNGVAA

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=429, Percent_Identity=43.8228438228438, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1790437, Length=426, Percent_Identity=42.018779342723, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI87082117, Length=246, Percent_Identity=53.2520325203252, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1790299, Length=461, Percent_Identity=37.3101952277657, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1788905, Length=347, Percent_Identity=42.3631123919308, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789087, Length=317, Percent_Identity=42.5867507886435, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789233, Length=316, Percent_Identity=43.9873417721519, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI87082152, Length=307, Percent_Identity=44.9511400651466, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=41.4925373134328, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=317, Percent_Identity=40.3785488958991, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=37.6543209876543, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1789828, Length=242, Percent_Identity=40.9090909090909, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=27.9742765273312, Blast_Score=125, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48686; Mature: 48686

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIIGSLSGELGVAARIAAARGATLAQAETVDAALECLRADARTDLIICDVTHDFGAL
CEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHH
MRRLAAERFTTAVVAAGAENDEKAAVDAIRAGAREYLPLPPDSDLIAAILSAVSTEQKGE
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHCCHHCCC
FIARDPAMRAVLSQADQVAPSTASILITGESGTGKEVLARYIHERSRRRKGPFVALNCAA
CHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
IPDTLLESELFGHEKGAFSGAIARRTGKFEAADRGTLLLDEIGEMDARLQAKLLRTLQER
CCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TVDRIGGDRPVPVDVRILAATNVDLRSAIADGRFREDLFFRLNVMTLRIPPLRQRPGDIA
HHHHCCCCCCCCEEEEEEEECCCHHHHHHHCCCHHHHHEEEEEEEEEECCCCCCCCCCCH
PLAHHFVAHYARLNEVGTKHLTSRAVERLLAYSWRGNVRELENTMHRAVLLSSGPMIDVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCEEEEE
HIGIPTEAEEPSYLLSPHAHATAGDTIGSLVGQRVEDVEQALILETLNHCLGNRTHAATI
ECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LGISIRALRNKLREYAARGAEVPAPHNGVAA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVLIIGSLSGELGVAARIAAARGATLAQAETVDAALECLRADARTDLIICDVTHDFGAL
CEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHH
MRRLAAERFTTAVVAAGAENDEKAAVDAIRAGAREYLPLPPDSDLIAAILSAVSTEQKGE
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHCCHHCCC
FIARDPAMRAVLSQADQVAPSTASILITGESGTGKEVLARYIHERSRRRKGPFVALNCAA
CHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
IPDTLLESELFGHEKGAFSGAIARRTGKFEAADRGTLLLDEIGEMDARLQAKLLRTLQER
CCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TVDRIGGDRPVPVDVRILAATNVDLRSAIADGRFREDLFFRLNVMTLRIPPLRQRPGDIA
HHHHCCCCCCCCEEEEEEEECCCHHHHHHHCCCHHHHHEEEEEEEEEECCCCCCCCCCCH
PLAHHFVAHYARLNEVGTKHLTSRAVERLLAYSWRGNVRELENTMHRAVLLSSGPMIDVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCEEEEE
HIGIPTEAEEPSYLLSPHAHATAGDTIGSLVGQRVEDVEQALILETLNHCLGNRTHAATI
ECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LGISIRALRNKLREYAARGAEVPAPHNGVAA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]