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The map label for this gene is nuoB2

Identifier: 148260123

GI number: 148260123

Start: 1247181

End: 1247876

Strand: Reverse

Name: nuoB2

Synonym: Acry_1119

Alternate gene names: 148260123

Gene position: 1247876-1247181 (Counterclockwise)

Preceding gene: 148260124

Following gene: 148260122

Centisome position: 36.82

GC content: 66.24

Gene sequence:

>696_bases
ATGCGCTTCACATTCGGCAAAGGCGCGACGGGACAACAGCTCTCCACGACCGCGCCTCTTCTCCGCGGCCTCGGCACAGC
CGGCCAGCCGGCCGCCCCATCCCCCGGCGGCGGCACCTTCACCGATGCGGTTCGCCGCAACGTGGCCCTCGGCCGGCTGG
AAGACATGGTGGCCTGGGGCCGCAAATATTCGATCTGGCCGTTCAATTTCGGCCTCTCCTGCTGCTATGTCGAAATGGCG
ACGGCCTTCACCAGCAAGTTCGACATCGCCCGTTTCGGCTCGGAAGTGCTGCGCGCCACCCCGCGCGAGGCCGACCTGAT
CGTCATCTCCGGTACCGTGTTCGTGAAGATGGCGCCCGTCATCAAGTATCTCTACGACCAGATGCTGGAGCCGCGCTGGG
TGATCTCGATGGGCGCCTGCGCCAATTCCGGCGGGATGTACGACATCTATGCCGTCGTCCAGGGTGCCGACAGTTTCCTG
CCCGTCGATGTCTATGTCCCCGGCTGTCCGCCCCGGCCGGACGCGCTGCTCGAAGGGCTGATGCTGCTGCAGAACTCGAT
CGGTTCCGAGCGCCGGCCGCTCAGCTGGCTGGTCGGTCCCAACGGCGCCGAGAAGATCGAGCGGCCCAGCTTCCGCGATC
TCAAACACCAGGAGAGGCAAGCGGCCGGCAGCCTCCCCACGCCGGACAGCGTTTAG

Upstream 100 bases:

>100_bases
CTCGCCCTGCTCGCCGCGCTCGCCTATCTGTGGCGCGCCGGCGCGCTCGAATGGGGGCCAAAGCCCCGCGCGCTTGCGAT
AGTGCGGAGGCGCTGACGCC

Downstream 100 bases:

>100_bases
GGAGGGCGGGATGCTGACGGAATTCAACAGCATCCCCGCCACAGAGGCCGAACAGGGTATCGTGTCCGACCTGGTCCGCA
CGCTCGGGCCGATCTCCTTC

Product: NADH-quinone oxidoreductase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRFTFGKGATGQQLSTTAPLLRGLGTAGQPAAPSPGGGTFTDAVRRNVALGRLEDMVAWGRKYSIWPFNFGLSCCYVEMA
TAFTSKFDIARFGSEVLRATPREADLIVISGTVFVKMAPVIKYLYDQMLEPRWVISMGACANSGGMYDIYAVVQGADSFL
PVDVYVPGCPPRPDALLEGLMLLQNSIGSERRPLSWLVGPNGAEKIERPSFRDLKHQERQAAGSLPTPDSV

Sequences:

>Translated_231_residues
MRFTFGKGATGQQLSTTAPLLRGLGTAGQPAAPSPGGGTFTDAVRRNVALGRLEDMVAWGRKYSIWPFNFGLSCCYVEMA
TAFTSKFDIARFGSEVLRATPREADLIVISGTVFVKMAPVIKYLYDQMLEPRWVISMGACANSGGMYDIYAVVQGADSFL
PVDVYVPGCPPRPDALLEGLMLLQNSIGSERRPLSWLVGPNGAEKIERPSFRDLKHQERQAAGSLPTPDSV
>Mature_231_residues
MRFTFGKGATGQQLSTTAPLLRGLGTAGQPAAPSPGGGTFTDAVRRNVALGRLEDMVAWGRKYSIWPFNFGLSCCYVEMA
TAFTSKFDIARFGSEVLRATPREADLIVISGTVFVKMAPVIKYLYDQMLEPRWVISMGACANSGGMYDIYAVVQGADSFL
PVDVYVPGCPPRPDALLEGLMLLQNSIGSERRPLSWLVGPNGAEKIERPSFRDLKHQERQAAGSLPTPDSV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=186, Percent_Identity=44.0860215053763, Blast_Score=172, Evalue=3e-43,
Organism=Escherichia coli, GI1788624, Length=188, Percent_Identity=68.0851063829787, Blast_Score=283, Evalue=6e-78,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=40.1639344262295, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788834, Length=156, Percent_Identity=34.6153846153846, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=189, Percent_Identity=43.3862433862434, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18859983, Length=149, Percent_Identity=51.6778523489933, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24642371, Length=149, Percent_Identity=51.6778523489933, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=51.3698630136986, Blast_Score=165, Evalue=2e-41,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB2_ACICJ (A5FXJ9)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001234250.1
- ProteinModelPortal:   A5FXJ9
- SMR:   A5FXJ9
- STRING:   A5FXJ9
- GeneID:   5162496
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_1119
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   PRPEAYI
- BioCyc:   ACRY349163:ACRY_1119-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 25040; Mature: 25040

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTFGKGATGQQLSTTAPLLRGLGTAGQPAAPSPGGGTFTDAVRRNVALGRLEDMVAWG
CEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
RKYSIWPFNFGLSCCYVEMATAFTSKFDIARFGSEVLRATPREADLIVISGTVFVKMAPV
CCCEECEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHEEHHHHH
IKYLYDQMLEPRWVISMGACANSGGMYDIYAVVQGADSFLPVDVYVPGCPPRPDALLEGL
HHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHH
MLLQNSIGSERRPLSWLVGPNGAEKIERPSFRDLKHQERQAAGSLPTPDSV
HHHHHHCCCCCCCCEEEECCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRFTFGKGATGQQLSTTAPLLRGLGTAGQPAAPSPGGGTFTDAVRRNVALGRLEDMVAWG
CEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
RKYSIWPFNFGLSCCYVEMATAFTSKFDIARFGSEVLRATPREADLIVISGTVFVKMAPV
CCCEECEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHEEHHHHH
IKYLYDQMLEPRWVISMGACANSGGMYDIYAVVQGADSFLPVDVYVPGCPPRPDALLEGL
HHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHH
MLLQNSIGSERRPLSWLVGPNGAEKIERPSFRDLKHQERQAAGSLPTPDSV
HHHHHHCCCCCCCCEEEECCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA