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The map label for this gene is 148260084

Identifier: 148260084

GI number: 148260084

Start: 1202181

End: 1202759

Strand: Reverse

Name: 148260084

Synonym: Acry_1077

Alternate gene names: NA

Gene position: 1202759-1202181 (Counterclockwise)

Preceding gene: 148260085

Following gene: 148260073

Centisome position: 35.49

GC content: 73.92

Gene sequence:

>579_bases
ATGACCTGGCGCGAGGCGGACAGCGCGACGCTGCTTGCCGGCCTGCGGTCGCTGCTCGCCGCGAAATCCATCGCCTCGGC
GGATGAAATCGCCGAGCGCATCCGCATCACCGATGGCGGCGGCCCGGCCCAGGGCGCGCGCATGGTCGCCAAGGCCTGGA
CCGACCCGGACTACAAGGCGCTGATGCTGGCGGACGGCGCCCGCGCCGCCGAGCTGCTCGACATCCCGATGCGCGGCCTG
CCGCCGCTCGGCGTGGTCGAGAACACGCCGGGCATCCACCACCTCGTCGTCTGCACCCTGTGCAGCTGCTACCCCCGCGC
GGTGCTCGGCTACCCGCCCTTCTGGTTCAAGTCCGCCGCCTACCGCGCCCGCGCGGTGCGCGACCCGCGCGGGCTGATCG
CCGAATGGGGCACCGAACTGCCGGCGGCCACCGCCATCCGCGTCGTCGACAGCACGGCCGATTACCGCTGGATGGTGCTG
CCGATGCGCCCCGCCGGCACCGAGGGCTGGTCCGAGGCCGAGCTCGCCGCCATCGTCCGCGAGGGCGACATGATCGGCGT
CACCCGCCCCCGCGCCTGA

Upstream 100 bases:

>100_bases
GCTTCCGCCAGGCCGATCTCTGGCCCGCCGCCGATCCCTGGCCGCATCCGGCCGGCGACGAGATCCTGATCGAGCTCTAC
GAACACTGGCTGGAGGCCGC

Downstream 100 bases:

>100_bases
CCGTCGCCCGGCCCCGGCTCACCGCGCCTGCATCGCCGGGTGCCGCGGCGGCTCGGCCGGCGCCGGCATCGGCATCGGTG
AGGTAATCTCCACCTGCGAG

Product: nitrile hydratase

Products: NA

Alternate protein names: L-NHase; L-nitrilase [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MTWREADSATLLAGLRSLLAAKSIASADEIAERIRITDGGGPAQGARMVAKAWTDPDYKALMLADGARAAELLDIPMRGL
PPLGVVENTPGIHHLVVCTLCSCYPRAVLGYPPFWFKSAAYRARAVRDPRGLIAEWGTELPAATAIRVVDSTADYRWMVL
PMRPAGTEGWSEAELAAIVREGDMIGVTRPRA

Sequences:

>Translated_192_residues
MTWREADSATLLAGLRSLLAAKSIASADEIAERIRITDGGGPAQGARMVAKAWTDPDYKALMLADGARAAELLDIPMRGL
PPLGVVENTPGIHHLVVCTLCSCYPRAVLGYPPFWFKSAAYRARAVRDPRGLIAEWGTELPAATAIRVVDSTADYRWMVL
PMRPAGTEGWSEAELAAIVREGDMIGVTRPRA
>Mature_191_residues
TWREADSATLLAGLRSLLAAKSIASADEIAERIRITDGGGPAQGARMVAKAWTDPDYKALMLADGARAAELLDIPMRGLP
PLGVVENTPGIHHLVVCTLCSCYPRAVLGYPPFWFKSAAYRARAVRDPRGLIAEWGTELPAATAIRVVDSTADYRWMVLP
MRPAGTEGWSEAELAAIVREGDMIGVTRPRA

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 20714; Mature: 20583

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWREADSATLLAGLRSLLAAKSIASADEIAERIRITDGGGPAQGARMVAKAWTDPDYKA
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCEE
LMLADGARAAELLDIPMRGLPPLGVVENTPGIHHLVVCTLCSCYPRAVLGYPPFWFKSAA
EEEECCCCHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
YRARAVRDPRGLIAEWGTELPAATAIRVVDSTADYRWMVLPMRPAGTEGWSEAELAAIVR
HHHHHHCCCHHHHHHHCCCCCHHHEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHH
EGDMIGVTRPRA
CCCEEECCCCCC
>Mature Secondary Structure 
TWREADSATLLAGLRSLLAAKSIASADEIAERIRITDGGGPAQGARMVAKAWTDPDYKA
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCEE
LMLADGARAAELLDIPMRGLPPLGVVENTPGIHHLVVCTLCSCYPRAVLGYPPFWFKSAA
EEEECCCCHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
YRARAVRDPRGLIAEWGTELPAATAIRVVDSTADYRWMVLPMRPAGTEGWSEAELAAIVR
HHHHHHCCCHHHHHHHCCCCCHHHEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHH
EGDMIGVTRPRA
CCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11700034 [H]