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The map label for this gene is dapE [H]

Identifier: 148259781

GI number: 148259781

Start: 860857

End: 862263

Strand: Reverse

Name: dapE [H]

Synonym: Acry_0769

Alternate gene names: 148259781

Gene position: 862263-860857 (Counterclockwise)

Preceding gene: 148259782

Following gene: 148259780

Centisome position: 25.44

GC content: 70.29

Gene sequence:

>1407_bases
ATGAGCACCAGCAGGATCGACGACGTTCTCGCCCATATCGACGCCCATCTCGACAGCGCGCGCGGGCGCTGGTTCGAGCT
GTTGCGGATTCCCTCGATCAGCGCGCAGGGGCTGACCGAAACCTCGCCGCACCGGGCGGATTGCCTGCGCGCCGCTTCAC
ATATGCGCGACCTGCTCGCGGGGATGGGATTCGCGGCACGGCTGTCGGAGACCAGGGGCCTGCCCGGCGTGATCGCGACG
CATCACAAGGCCGGGCCGGATGCGCCGACCGTGCTGTATTACGGGCATTACGACGTGCAGCCCGCCGATCCGCTGGACCT
CTGGCACAGCGATCCGTTCGACCCGCAGCTTGTGGACCATCCGGAAGGCCCGCGCGTGGTCGCCCGCGGCGCGGTGGACG
ACAAGGGCCAGGTGATGATGGTGCTGGAGGCGGTGCGCGCCTGGCTCGACGTGACGGGCGGCCTGCCGGTGAACCTCACC
GTGCTGCTGGAGGGCGAGGAGGAATGCGGCAGCCCCTCGCTCGCCACCATGCTCGAGGCCGAGCGCGACGCGCTGAAGGC
CGATTTCGTGCTCGTCGCCGACACCAACATGTGGGACCGGCAGACGCCGGCGATCACCACCAGCCTGCGCGGGCTCGCGG
CGGTGGAGGTGCGGATTTCGGGGCCGGACCGCGACCTGCATTCCGGCCTGTTCGGCGGGCCGGCGCTCAACCCGATCCGC
GCGCTGACCCGCATTCTCGGCGACCTGTTCGACGCCGACGGGCGGATCCGGATTCCGGGCTTCTATGACGGCATCCTCGA
TATTCCGGCGGACGTGCTCGCCTCCTGGCGGGCGACGGGGTTCGACGAGGCCGCCTGGCTCGGCGGCATCGGCCTCGCCC
GGGCGAGCGGCGAGCGCGGGTTTTCGGTGCTGGAGCAGAACTGGGCGCGGCCGACCGCCGAGATCAACGGCATTTTCGGC
GGCTATCAGGGCGAGGGCACCAAGACGGTGATTCCCGCCGAGGCCGGCGCCAAGCTCACCTTCCGCCTGGTGCCGGGCCA
GGACCCGGCGAAGGTGGTCGAGGGGTTCAGGGAATTCGTTACCGCGCGGCTGCTGCCGGATGCGAAGGCGCGTTTCGAGG
TGCATGGCGCCTCGGCCGGGCTGGTCGTGCCGGTCGACCTGCCCTTCGTCCGCGCGGCGCGGGCGGCGCTGGAGCAGGAA
TTCGGCCGCAAGCCGGTGCTCGCCGGATGCGGTGCGTCGATTCCGGTGGTCGAGGCGTTCGAGCGGCAGCTCGGGCTGAA
GACGCTGCTGGTCGGCTTCGGGCTGGACGATGACTGCATCCACAGCCCGAACGAGAAATTCGAGATGCGCTGCTTCCATC
GCGGCATGCGGGCCGAGGCGCGGATGCTTGCCGCCTTCGCCGGCTGA

Upstream 100 bases:

>100_bases
TCCGCCTCGCGGAGGCGATCGACGGGCTGGCTTGAGCGCCGCTGTCTTTTCCGGCACGCCGCCCTCCCCTATCGTCGTGC
CGCCAACAGAGGGACATTCG

Downstream 100 bases:

>100_bases
GGCGGTGGCGTCATTCCTGGTGGCGGCCGGCCTGCGCGGGGCCGCGGGCGGGCCGTTCGACCTCGAACTCGGGCGCGGCG
AGGCGGTGGCGATCACCGGC

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSTSRIDDVLAHIDAHLDSARGRWFELLRIPSISAQGLTETSPHRADCLRAASHMRDLLAGMGFAARLSETRGLPGVIAT
HHKAGPDAPTVLYYGHYDVQPADPLDLWHSDPFDPQLVDHPEGPRVVARGAVDDKGQVMMVLEAVRAWLDVTGGLPVNLT
VLLEGEEECGSPSLATMLEAERDALKADFVLVADTNMWDRQTPAITTSLRGLAAVEVRISGPDRDLHSGLFGGPALNPIR
ALTRILGDLFDADGRIRIPGFYDGILDIPADVLASWRATGFDEAAWLGGIGLARASGERGFSVLEQNWARPTAEINGIFG
GYQGEGTKTVIPAEAGAKLTFRLVPGQDPAKVVEGFREFVTARLLPDAKARFEVHGASAGLVVPVDLPFVRAARAALEQE
FGRKPVLAGCGASIPVVEAFERQLGLKTLLVGFGLDDDCIHSPNEKFEMRCFHRGMRAEARMLAAFAG

Sequences:

>Translated_468_residues
MSTSRIDDVLAHIDAHLDSARGRWFELLRIPSISAQGLTETSPHRADCLRAASHMRDLLAGMGFAARLSETRGLPGVIAT
HHKAGPDAPTVLYYGHYDVQPADPLDLWHSDPFDPQLVDHPEGPRVVARGAVDDKGQVMMVLEAVRAWLDVTGGLPVNLT
VLLEGEEECGSPSLATMLEAERDALKADFVLVADTNMWDRQTPAITTSLRGLAAVEVRISGPDRDLHSGLFGGPALNPIR
ALTRILGDLFDADGRIRIPGFYDGILDIPADVLASWRATGFDEAAWLGGIGLARASGERGFSVLEQNWARPTAEINGIFG
GYQGEGTKTVIPAEAGAKLTFRLVPGQDPAKVVEGFREFVTARLLPDAKARFEVHGASAGLVVPVDLPFVRAARAALEQE
FGRKPVLAGCGASIPVVEAFERQLGLKTLLVGFGLDDDCIHSPNEKFEMRCFHRGMRAEARMLAAFAG
>Mature_467_residues
STSRIDDVLAHIDAHLDSARGRWFELLRIPSISAQGLTETSPHRADCLRAASHMRDLLAGMGFAARLSETRGLPGVIATH
HKAGPDAPTVLYYGHYDVQPADPLDLWHSDPFDPQLVDHPEGPRVVARGAVDDKGQVMMVLEAVRAWLDVTGGLPVNLTV
LLEGEEECGSPSLATMLEAERDALKADFVLVADTNMWDRQTPAITTSLRGLAAVEVRISGPDRDLHSGLFGGPALNPIRA
LTRILGDLFDADGRIRIPGFYDGILDIPADVLASWRATGFDEAAWLGGIGLARASGERGFSVLEQNWARPTAEINGIFGG
YQGEGTKTVIPAEAGAKLTFRLVPGQDPAKVVEGFREFVTARLLPDAKARFEVHGASAGLVVPVDLPFVRAARAALEQEF
GRKPVLAGCGASIPVVEAFERQLGLKTLLVGFGLDDDCIHSPNEKFEMRCFHRGMRAEARMLAAFAG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI21071039, Length=487, Percent_Identity=30.5954825462012, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI271398239, Length=398, Percent_Identity=31.4070351758794, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI271398277, Length=317, Percent_Identity=30.2839116719243, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17563146, Length=471, Percent_Identity=31.6348195329087, Blast_Score=215, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI193211059, Length=478, Percent_Identity=28.6610878661088, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321159, Length=480, Percent_Identity=29.1666666666667, Blast_Score=192, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6319758, Length=372, Percent_Identity=25.2688172043011, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585880, Length=472, Percent_Identity=29.6610169491525, Blast_Score=193, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50414; Mature: 50283

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSRIDDVLAHIDAHLDSARGRWFELLRIPSISAQGLTETSPHRADCLRAASHMRDLLA
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GMGFAARLSETRGLPGVIATHHKAGPDAPTVLYYGHYDVQPADPLDLWHSDPFDPQLVDH
HCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCC
PEGPRVVARGAVDDKGQVMMVLEAVRAWLDVTGGLPVNLTVLLEGEEECGSPSLATMLEA
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCHHCCCCHHHHHHHH
ERDALKADFVLVADTNMWDRQTPAITTSLRGLAAVEVRISGPDRDLHSGLFGGPALNPIR
HHHHHHCCEEEEECCCCCCCCCCCHHHHCCCEEEEEEEECCCCCHHHCCCCCCCCCCHHH
ALTRILGDLFDADGRIRIPGFYDGILDIPADVLASWRATGFDEAAWLGGIGLARASGERG
HHHHHHHHHHCCCCCEECCCHHCCHHHCCHHHHHHHHCCCCCCHHHHCCCHHCCCCCCCC
FSVLEQNWARPTAEINGIFGGYQGEGTKTVIPAEAGAKLTFRLVPGQDPAKVVEGFREFV
HHHHHHHCCCCCHHHCCEECCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHH
TARLLPDAKARFEVHGASAGLVVPVDLPFVRAARAALEQEFGRKPVLAGCGASIPVVEAF
HHHCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHH
ERQLGLKTLLVGFGLDDDCIHSPNEKFEMRCFHRGMRAEARMLAAFAG
HHHHCHHHHHEECCCCHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
STSRIDDVLAHIDAHLDSARGRWFELLRIPSISAQGLTETSPHRADCLRAASHMRDLLA
CCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GMGFAARLSETRGLPGVIATHHKAGPDAPTVLYYGHYDVQPADPLDLWHSDPFDPQLVDH
HCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCC
PEGPRVVARGAVDDKGQVMMVLEAVRAWLDVTGGLPVNLTVLLEGEEECGSPSLATMLEA
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCHHCCCCHHHHHHHH
ERDALKADFVLVADTNMWDRQTPAITTSLRGLAAVEVRISGPDRDLHSGLFGGPALNPIR
HHHHHHCCEEEEECCCCCCCCCCCHHHHCCCEEEEEEEECCCCCHHHCCCCCCCCCCHHH
ALTRILGDLFDADGRIRIPGFYDGILDIPADVLASWRATGFDEAAWLGGIGLARASGERG
HHHHHHHHHHCCCCCEECCCHHCCHHHCCHHHHHHHHCCCCCCHHHHCCCHHCCCCCCCC
FSVLEQNWARPTAEINGIFGGYQGEGTKTVIPAEAGAKLTFRLVPGQDPAKVVEGFREFV
HHHHHHHCCCCCHHHCCEECCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHH
TARLLPDAKARFEVHGASAGLVVPVDLPFVRAARAALEQEFGRKPVLAGCGASIPVVEAF
HHHCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHH
ERQLGLKTLLVGFGLDDDCIHSPNEKFEMRCFHRGMRAEARMLAAFAG
HHHHCHHHHHEECCCCHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA