Click here to switch to the map view.

The map label for this gene is pyrB

Identifier: 148259745

GI number: 148259745

Start: 820351

End: 821286

Strand: Reverse

Name: pyrB

Synonym: Acry_0733

Alternate gene names: 148259745

Gene position: 821286-820351 (Counterclockwise)

Preceding gene: 148259746

Following gene: 148259744

Centisome position: 24.23

GC content: 69.76

Gene sequence:

>936_bases
ATGCGGTTGCCGACGCGCCATCTCCTGGGAATCGAAGGTCTCCACCCGCGCGACATTTCCGCCCTGCTCGACCTCGCCGA
GAGCTACGTGCTGCTCAACCGGTCGGGCAAGACGCGGCGGGACGTGCTGCGCGGCCGGACCCTGATCAACCTGTTCTTCG
AGGATTCCACCCGCACCCGCACGAGCTTCGAGCTCGCCGGCAAGCGGCTCGGCGCCGATGTGATCAACATGTCGGTCTCG
ACCTCCTCGGTCTCCAAGGGCGAGACCCTGCTCGACACCGCGGCGACGCTGAACGCGATGAACTGCGACCTGCTGGTGGT
CCGCCACAAGGCCTCCGGCGCGCCCGCCCTGCTCGCCCAGAAGGTGGATGCGGCGGTGATCAACGCCGGCGACGGCATGC
ACGAGCACCCGACCCAGGCGCTGCTCGACGCGCTGACGATCCGCCGCAACAAGGGCACGCTCGCGGGCCTCACCGTCGCC
ATCTGCGGCGACATCGCGCATTCCCGCGTCGCCCGCTCGAACCTGCTGCTGCTCACCGCGATGGGCAGCCGGGTGCGCGT
GGTCGGCCCGCCGACGCTGATCCCCTCCGGCATCGACCAGTTCGGCGCCACCGTCTTCCACGACATGCGCGAGGGCCTGC
GCGACGCCGATATCGTGATGGCGCTGCGGTTGCAGACCGAGCGGATGAGCGCCGGGCTGATCCCCAGCGCGCGCGAGTTC
TTCACCTTCTACGGGCTCGACGCCGCCAAGCTCGCGATGGCGAAGCCGGACGCGCTGGTGATGCATCCCGGCCCGATGAA
TCGCGGCGTCGAGATCGACAGCCAGGTCGCCGACGACGCCACCCGCTCGGTGATCCGCGAGCAGGTGGAGATGGGCGTCG
CCATCCGCATGGCGGTGCTCGACGTGCTGGCCCGGACGGCGCTCGCCCATGCATGA

Upstream 100 bases:

>100_bases
CTTCATGACCGAGGAGATCGGCGCCCTTCACCGCATCGTCTGGGCCAGCCAGTAGGAACCGCTCTTCCCGCCGGCGCGGC
GGGGCTGTAAGGAGACCGTC

Downstream 100 bases:

>100_bases
CACGCCCCCCGACACGCTGATCCGCGGCGCGCGGATCGTCGATCCCGCCACCGGCCACGACGCGGTGGGCGACCTGCTGG
TCCGCGACGGCGTCATCGCC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MRLPTRHLLGIEGLHPRDISALLDLAESYVLLNRSGKTRRDVLRGRTLINLFFEDSTRTRTSFELAGKRLGADVINMSVS
TSSVSKGETLLDTAATLNAMNCDLLVVRHKASGAPALLAQKVDAAVINAGDGMHEHPTQALLDALTIRRNKGTLAGLTVA
ICGDIAHSRVARSNLLLLTAMGSRVRVVGPPTLIPSGIDQFGATVFHDMREGLRDADIVMALRLQTERMSAGLIPSAREF
FTFYGLDAAKLAMAKPDALVMHPGPMNRGVEIDSQVADDATRSVIREQVEMGVAIRMAVLDVLARTALAHA

Sequences:

>Translated_311_residues
MRLPTRHLLGIEGLHPRDISALLDLAESYVLLNRSGKTRRDVLRGRTLINLFFEDSTRTRTSFELAGKRLGADVINMSVS
TSSVSKGETLLDTAATLNAMNCDLLVVRHKASGAPALLAQKVDAAVINAGDGMHEHPTQALLDALTIRRNKGTLAGLTVA
ICGDIAHSRVARSNLLLLTAMGSRVRVVGPPTLIPSGIDQFGATVFHDMREGLRDADIVMALRLQTERMSAGLIPSAREF
FTFYGLDAAKLAMAKPDALVMHPGPMNRGVEIDSQVADDATRSVIREQVEMGVAIRMAVLDVLARTALAHA
>Mature_311_residues
MRLPTRHLLGIEGLHPRDISALLDLAESYVLLNRSGKTRRDVLRGRTLINLFFEDSTRTRTSFELAGKRLGADVINMSVS
TSSVSKGETLLDTAATLNAMNCDLLVVRHKASGAPALLAQKVDAAVINAGDGMHEHPTQALLDALTIRRNKGTLAGLTVA
ICGDIAHSRVARSNLLLLTAMGSRVRVVGPPTLIPSGIDQFGATVFHDMREGLRDADIVMALRLQTERMSAGLIPSAREF
FTFYGLDAAKLAMAKPDALVMHPGPMNRGVEIDSQVADDATRSVIREQVEMGVAIRMAVLDVLARTALAHA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=37.2990353697749, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790703, Length=227, Percent_Identity=29.5154185022026, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786469, Length=196, Percent_Identity=30.1020408163265, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=328, Percent_Identity=34.7560975609756, Blast_Score=140, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6322331, Length=309, Percent_Identity=34.6278317152104, Blast_Score=149, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24642586, Length=305, Percent_Identity=34.0983606557377, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_ACICJ (A5FWH1)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001233872.1
- ProteinModelPortal:   A5FWH1
- SMR:   A5FWH1
- STRING:   A5FWH1
- GeneID:   5162211
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_0733
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   ACRY349163:ACRY_0733-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33475; Mature: 33475

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPTRHLLGIEGLHPRDISALLDLAESYVLLNRSGKTRRDVLRGRTLINLFFEDSTRTR
CCCCCCHHCCCCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHCCEEEEEEECCCCCCH
TSFELAGKRLGADVINMSVSTSSVSKGETLLDTAATLNAMNCDLLVVRHKASGAPALLAQ
HHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHH
KVDAAVINAGDGMHEHPTQALLDALTIRRNKGTLAGLTVAICGDIAHSRVARSNLLLLTA
HHHHEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEE
MGSRVRVVGPPTLIPSGIDQFGATVFHDMREGLRDADIVMALRLQTERMSAGLIPSAREF
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHEEHHHHHHHHCCCCCCHHHH
FTFYGLDAAKLAMAKPDALVMHPGPMNRGVEIDSQVADDATRSVIREQVEMGVAIRMAVL
HHHHCCCHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVLARTALAHA
HHHHHHHHHCC
>Mature Secondary Structure
MRLPTRHLLGIEGLHPRDISALLDLAESYVLLNRSGKTRRDVLRGRTLINLFFEDSTRTR
CCCCCCHHCCCCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHCCEEEEEEECCCCCCH
TSFELAGKRLGADVINMSVSTSSVSKGETLLDTAATLNAMNCDLLVVRHKASGAPALLAQ
HHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHH
KVDAAVINAGDGMHEHPTQALLDALTIRRNKGTLAGLTVAICGDIAHSRVARSNLLLLTA
HHHHEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEE
MGSRVRVVGPPTLIPSGIDQFGATVFHDMREGLRDADIVMALRLQTERMSAGLIPSAREF
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHEEHHHHHHHHCCCCCCHHHH
FTFYGLDAAKLAMAKPDALVMHPGPMNRGVEIDSQVADDATRSVIREQVEMGVAIRMAVL
HHHHCCCHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVLARTALAHA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA