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The map label for this gene is fixK [H]

Identifier: 148259592

GI number: 148259592

Start: 647763

End: 648470

Strand: Reverse

Name: fixK [H]

Synonym: Acry_0576

Alternate gene names: 148259592

Gene position: 648470-647763 (Counterclockwise)

Preceding gene: 148259594

Following gene: 148259591

Centisome position: 19.13

GC content: 65.96

Gene sequence:

>708_bases
ATGTTGCATGTCGCTGCCGAGCCGTCTCGGTCCACGATTGCCAACTGGAGTGCCGCCGGTGAGCCGGGCTTTCGCGCAGG
TGCGCTGGAGGCCGGCGCCTCGCTGTTCATCGAAGGCGAAACGGTGGACAAGATCTACGATCTCGTGTCCGGCATCGTCC
GGCTGTCGAAGATGCTGCCCGACGGGCGCCGGCAGATCCTGGGCTTCCTGTTTCCGGGCGAAATCTTTACGCCGTCCGGC
TTCGCCATCAGCCATGGCGGCTGCGCGACATGCTCCGCGGATGCCGTGACCGCGGTCGAGGTCGTGACGCATCGGCTCAG
CAGTTTCGATGGGCTGCTGGCCAGCCGGCCGGAACTGACGCGCAGCCTGCTGGCCACAGCCGCGAAGAGCCTTTTCATGG
CGCAGCAACAGATGCTGCTGCTCGGCCGGATGAGCGCGACCGAACGCCTGGTCTGGTTCCTGCTGCGGATGGCCGAACAG
CAGGATGTCGAAGCCGGGGAACCGATCCGCCTGCCGATGTCCCGTGTGGATATCGCGGATTACCTCGGGCTGACGATGGA
AACCGTCTCCCGCGTCTTCCGCTGGCTGCGCGAGCGCAACATCATCGCCCTCAAGGGGGCGACGGAAATCCGGATCGTCG
ATCCGGGACGGGCGCGCCAGATCGCCGATTGCGCCTGCAGCGCGACAATGCCGCGCTGCAGGCAATAG

Upstream 100 bases:

>100_bases
AGATTGGCTGTTCCGGCATCGGCGTGTTGCAGTGAGCGTTTAGGAGTGACGGCGCTGAGTCTCGAGCCCTATCAACCCTG
GACGCATGAGGACCACGCCA

Downstream 100 bases:

>100_bases
GGTCCCCGGATCGGATCAGGATGCTCGCGTTGTCACGACGATGGCGCACCGCGTCCGATCCGGACGGACCGGAAAATGCT
CCAGACGAAACAGGTCCCAC

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MLHVAAEPSRSTIANWSAAGEPGFRAGALEAGASLFIEGETVDKIYDLVSGIVRLSKMLPDGRRQILGFLFPGEIFTPSG
FAISHGGCATCSADAVTAVEVVTHRLSSFDGLLASRPELTRSLLATAAKSLFMAQQQMLLLGRMSATERLVWFLLRMAEQ
QDVEAGEPIRLPMSRVDIADYLGLTMETVSRVFRWLRERNIIALKGATEIRIVDPGRARQIADCACSATMPRCRQ

Sequences:

>Translated_235_residues
MLHVAAEPSRSTIANWSAAGEPGFRAGALEAGASLFIEGETVDKIYDLVSGIVRLSKMLPDGRRQILGFLFPGEIFTPSG
FAISHGGCATCSADAVTAVEVVTHRLSSFDGLLASRPELTRSLLATAAKSLFMAQQQMLLLGRMSATERLVWFLLRMAEQ
QDVEAGEPIRLPMSRVDIADYLGLTMETVSRVFRWLRERNIIALKGATEIRIVDPGRARQIADCACSATMPRCRQ
>Mature_235_residues
MLHVAAEPSRSTIANWSAAGEPGFRAGALEAGASLFIEGETVDKIYDLVSGIVRLSKMLPDGRRQILGFLFPGEIFTPSG
FAISHGGCATCSADAVTAVEVVTHRLSSFDGLLASRPELTRSLLATAAKSLFMAQQQMLLLGRMSATERLVWFLLRMAEQ
QDVEAGEPIRLPMSRVDIADYLGLTMETVSRVFRWLRERNIIALKGATEIRIVDPGRARQIADCACSATMPRCRQ

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=184, Percent_Identity=28.2608695652174, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789756, Length=177, Percent_Identity=25.9887005649718, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25628; Mature: 25628

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHVAAEPSRSTIANWSAAGEPGFRAGALEAGASLFIEGETVDKIYDLVSGIVRLSKMLP
CCCCCCCCCCHHHCCCCCCCCCCCCCHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCC
DGRRQILGFLFPGEIFTPSGFAISHGGCATCSADAVTAVEVVTHRLSSFDGLLASRPELT
CHHHHHHHHCCCCCEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
RSLLATAAKSLFMAQQQMLLLGRMSATERLVWFLLRMAEQQDVEAGEPIRLPMSRVDIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
YLGLTMETVSRVFRWLRERNIIALKGATEIRIVDPGRARQIADCACSATMPRCRQ
HHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLHVAAEPSRSTIANWSAAGEPGFRAGALEAGASLFIEGETVDKIYDLVSGIVRLSKMLP
CCCCCCCCCCHHHCCCCCCCCCCCCCHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCC
DGRRQILGFLFPGEIFTPSGFAISHGGCATCSADAVTAVEVVTHRLSSFDGLLASRPELT
CHHHHHHHHCCCCCEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
RSLLATAAKSLFMAQQQMLLLGRMSATERLVWFLLRMAEQQDVEAGEPIRLPMSRVDIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
YLGLTMETVSRVFRWLRERNIIALKGATEIRIVDPGRARQIADCACSATMPRCRQ
HHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2663474; 11481432 [H]