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The map label for this gene is purR [H]

Identifier: 148259517

GI number: 148259517

Start: 557574

End: 558581

Strand: Reverse

Name: purR [H]

Synonym: Acry_0501

Alternate gene names: 148259517

Gene position: 558581-557574 (Counterclockwise)

Preceding gene: 148259518

Following gene: 148259513

Centisome position: 16.48

GC content: 62.1

Gene sequence:

>1008_bases
TTGGCCACCATGGAAGATGTTGCCCACAAGGCAGGCGTTTCGGTATCAACCGTTTCGCATGTCATCAACGGCACCAGAAA
AGTCCGTCCCGCGACGGTCGAAGCTGTAAGGGATGCCATCAGGAAGACCGGCTACGTTCCGAACTCGCTGGCGCAGGCCC
TGGCGGGAGCGTCGACGAAAACGATCGGTGTGGCGATCTCGGCCCTGACAAATCATTATTTTGCGGAAACGGTCCGTGTG
ATCGAATCCGAATGCGCAAGGCGGGGCCTGATGATATTCCTGGCCGACACGCATGACGATCCGGACCAGGAACTGCGGGT
CATCAAGGCGCTGCACCAGCGCCGGGTGGACGGCATCATTCTCGCACCGACCGCGGGAACCGACCGACGGGCGCTCGACT
ACCTGAAGGGCAAGGGCATACCGTCGGTGCTGGTCGATCGTATCGTTCGAGGCGAGTTTGACCAGGTCGGCGTCGAGAAC
ACCGTCGCGTGCCGCGAGCTGATCTCGCATCTGATCGGCCACGGCCATCGCCGCATCGGCTTCGTGGCGGGAGCCCCAGG
AATTTCAACATCGATCGAGCGGCTCGAAGGATATCGCGACGCCCTGCATCAGGCCGGCATCCCGTTCGATCCCACCTTGG
TGTGTTGCGGAGAATCAGCAAACGAACCGGCACGCCGCGCCGTTCGCGAACTGCTCCGGCTCGACCCACGGCCGACCGCG
ATCGTGGCCTCCAACAACCTGATGATGATCGGCGCCATGCACGCCTTGCGCGACGCCGGCATAGAGGTGCCTGCGGGAAT
GGCTCTGGTCGGGTTCGATGATTTCGACTGGGCCGAACTCTTCAACCCCCGGCTCACAGTACTCGCACAGCCACTCGAGG
ACATCGCCATGAAGGCGGTGGAACTGCTGACGAGACGCATCGCGCGGCCCGATGATCAATTCGAAATCGTGCGTCTCACG
CCGGAATTGAGGATCCGGAACTCCTGCGGCTGCCAGGATCAGCGCTGA

Upstream 100 bases:

>100_bases
CATCATGATTGGACAGTATGGGAACGTCGCCCCGCTCCCATCTCCTCGGTCGGCTGCTTACCGACATCACCGGTCGCCGG
ATCTGTAAGGGGTCGAGCAA

Downstream 100 bases:

>100_bases
CACCGCCGGGGCCCGGCCAAAGCCTGGCCACGGCGGTGTCACAAGAACGCATCCGAGGTCAGCGCACCTGACGCTCGACG
GGCAGCGCGGCGTTGCGCGA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MATMEDVAHKAGVSVSTVSHVINGTRKVRPATVEAVRDAIRKTGYVPNSLAQALAGASTKTIGVAISALTNHYFAETVRV
IESECARRGLMIFLADTHDDPDQELRVIKALHQRRVDGIILAPTAGTDRRALDYLKGKGIPSVLVDRIVRGEFDQVGVEN
TVACRELISHLIGHGHRRIGFVAGAPGISTSIERLEGYRDALHQAGIPFDPTLVCCGESANEPARRAVRELLRLDPRPTA
IVASNNLMMIGAMHALRDAGIEVPAGMALVGFDDFDWAELFNPRLTVLAQPLEDIAMKAVELLTRRIARPDDQFEIVRLT
PELRIRNSCGCQDQR

Sequences:

>Translated_335_residues
MATMEDVAHKAGVSVSTVSHVINGTRKVRPATVEAVRDAIRKTGYVPNSLAQALAGASTKTIGVAISALTNHYFAETVRV
IESECARRGLMIFLADTHDDPDQELRVIKALHQRRVDGIILAPTAGTDRRALDYLKGKGIPSVLVDRIVRGEFDQVGVEN
TVACRELISHLIGHGHRRIGFVAGAPGISTSIERLEGYRDALHQAGIPFDPTLVCCGESANEPARRAVRELLRLDPRPTA
IVASNNLMMIGAMHALRDAGIEVPAGMALVGFDDFDWAELFNPRLTVLAQPLEDIAMKAVELLTRRIARPDDQFEIVRLT
PELRIRNSCGCQDQR
>Mature_334_residues
ATMEDVAHKAGVSVSTVSHVINGTRKVRPATVEAVRDAIRKTGYVPNSLAQALAGASTKTIGVAISALTNHYFAETVRVI
ESECARRGLMIFLADTHDDPDQELRVIKALHQRRVDGIILAPTAGTDRRALDYLKGKGIPSVLVDRIVRGEFDQVGVENT
VACRELISHLIGHGHRRIGFVAGAPGISTSIERLEGYRDALHQAGIPFDPTLVCCGESANEPARRAVRELLRLDPRPTAI
VASNNLMMIGAMHALRDAGIEVPAGMALVGFDDFDWAELFNPRLTVLAQPLEDIAMKAVELLTRRIARPDDQFEIVRLTP
ELRIRNSCGCQDQR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=35.258358662614, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=30.3030303030303, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1790369, Length=304, Percent_Identity=30.2631578947368, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1789202, Length=300, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789068, Length=298, Percent_Identity=32.8859060402685, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788474, Length=304, Percent_Identity=31.5789473684211, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787906, Length=259, Percent_Identity=33.976833976834, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994940, Length=306, Percent_Identity=29.7385620915033, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=27.1875, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786268, Length=312, Percent_Identity=26.9230769230769, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786540, Length=303, Percent_Identity=28.0528052805281, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=26.625386996904, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790689, Length=262, Percent_Identity=24.0458015267176, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36574; Mature: 36442

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMEDVAHKAGVSVSTVSHVINGTRKVRPATVEAVRDAIRKTGYVPNSLAQALAGASTK
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCH
TIGVAISALTNHYFAETVRVIESECARRGLMIFLADTHDDPDQELRVIKALHQRRVDGII
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
LAPTAGTDRRALDYLKGKGIPSVLVDRIVRGEFDQVGVENTVACRELISHLIGHGHRRIG
EECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCCCCEEE
FVAGAPGISTSIERLEGYRDALHQAGIPFDPTLVCCGESANEPARRAVRELLRLDPRPTA
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCE
IVASNNLMMIGAMHALRDAGIEVPAGMALVGFDDFDWAELFNPRLTVLAQPLEDIAMKAV
EEECCCEEEEHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCCHHHHHHCCHHHHHHHHH
ELLTRRIARPDDQFEIVRLTPELRIRNSCGCQDQR
HHHHHHHCCCCCCEEEEEECCCCEECCCCCCCCCC
>Mature Secondary Structure 
ATMEDVAHKAGVSVSTVSHVINGTRKVRPATVEAVRDAIRKTGYVPNSLAQALAGASTK
CCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCH
TIGVAISALTNHYFAETVRVIESECARRGLMIFLADTHDDPDQELRVIKALHQRRVDGII
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
LAPTAGTDRRALDYLKGKGIPSVLVDRIVRGEFDQVGVENTVACRELISHLIGHGHRRIG
EECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCCCCEEE
FVAGAPGISTSIERLEGYRDALHQAGIPFDPTLVCCGESANEPARRAVRELLRLDPRPTA
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCE
IVASNNLMMIGAMHALRDAGIEVPAGMALVGFDDFDWAELFNPRLTVLAQPLEDIAMKAV
EEECCCEEEEHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCCHHHHHHCCHHHHHHHHH
ELLTRRIARPDDQFEIVRLTPELRIRNSCGCQDQR
HHHHHHHCCCCCCEEEEEECCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA