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The map label for this gene is xthA [H]

Identifier: 148259431

GI number: 148259431

Start: 468480

End: 469283

Strand: Reverse

Name: xthA [H]

Synonym: Acry_0414

Alternate gene names: 148259431

Gene position: 469283-468480 (Counterclockwise)

Preceding gene: 148259432

Following gene: 148259425

Centisome position: 13.85

GC content: 59.7

Gene sequence:

>804_bases
ATGACCGCTCTGACCGTCGCGACCTGGAACATAAATTCGGTTCGACTCCGTCGGGATCTGCTGCACAGTCTCATGGATCA
GGCCGCGCCCGATATCGTTTGCCTGCAGGAAACCAAGGTCCCCGACGACCTTTTCCCGGAAGACCTCGGCACGGCACTCG
GTCTGCCTCATGTGCTCAAACGCGGCATGAAAGGTTATAACGGCGTTGCGATCCTCTCCCGCCTTCCGCTTACAGCGGTC
GAGGATACGCCCGACTGGTGCATGAAATCGGACTGCCGGCACATTGCCGCGCGAATCGATACCGGAACCGATCCGCTTGT
GCTGCACAATTTCTACGTCCCTGCCGGCGGCGATATCCCTGATCGGGCAGAGAACCCGAAATATGGGCACAAGCTCGATT
TCATTGCGGAAGCCCGCGACCACTTCGCAATGAACCGCCCGCATCGCACCATGCTGGTGGGTGATCTGAATATCGCGCCG
CTGGAAAACGATGTCTGGAGCCACAAGCAGTTATTGAACGTCGTTAGCCATACGCCGCCGGAAACCGAGGGTCTGAACGC
CTGGCGGACCGAAGGTTTCATCGACGCCATCCGGCATTTCGTCCCCGATGACCAGAAGCTTTTCACCTGGTGGTCTTATC
GGAACAGGGACTGGCGGGCCTCCAACAGGGGCCGGCGACTCGATCACATCTGGGTCTCGACGGACCTGATCGGCAATCTG
GCGCGCCATGCCATTCTTCAGGACGCCCGGGACTGGGAACGCGGTTCCGATCACGTCCCGGTGGTGTTGACCCTCGACCT
GTAG

Upstream 100 bases:

>100_bases
TGATCTATACCGGCACGCCCGATGGCGTCGGCCAGATCCAACCTGGCGACCGCGTTTACGGCGTCATTGATGGCGTCGGC
GAACTGGAATTCACCCTCGC

Downstream 100 bases:

>100_bases
AACTCGAGCGCGCGCTGCAGAGTCCGGCGCGCGGCGGCGGCTTCACGGCCAAGCGCGATCAGATCCGGGATCTGCGCGGG
CCGGCGGAAAACGCTTTTCA

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTALTVATWNINSVRLRRDLLHSLMDQAAPDIVCLQETKVPDDLFPEDLGTALGLPHVLKRGMKGYNGVAILSRLPLTAV
EDTPDWCMKSDCRHIAARIDTGTDPLVLHNFYVPAGGDIPDRAENPKYGHKLDFIAEARDHFAMNRPHRTMLVGDLNIAP
LENDVWSHKQLLNVVSHTPPETEGLNAWRTEGFIDAIRHFVPDDQKLFTWWSYRNRDWRASNRGRRLDHIWVSTDLIGNL
ARHAILQDARDWERGSDHVPVVLTLDL

Sequences:

>Translated_267_residues
MTALTVATWNINSVRLRRDLLHSLMDQAAPDIVCLQETKVPDDLFPEDLGTALGLPHVLKRGMKGYNGVAILSRLPLTAV
EDTPDWCMKSDCRHIAARIDTGTDPLVLHNFYVPAGGDIPDRAENPKYGHKLDFIAEARDHFAMNRPHRTMLVGDLNIAP
LENDVWSHKQLLNVVSHTPPETEGLNAWRTEGFIDAIRHFVPDDQKLFTWWSYRNRDWRASNRGRRLDHIWVSTDLIGNL
ARHAILQDARDWERGSDHVPVVLTLDL
>Mature_266_residues
TALTVATWNINSVRLRRDLLHSLMDQAAPDIVCLQETKVPDDLFPEDLGTALGLPHVLKRGMKGYNGVAILSRLPLTAVE
DTPDWCMKSDCRHIAARIDTGTDPLVLHNFYVPAGGDIPDRAENPKYGHKLDFIAEARDHFAMNRPHRTMLVGDLNIAPL
ENDVWSHKQLLNVVSHTPPETEGLNAWRTEGFIDAIRHFVPDDQKLFTWWSYRNRDWRASNRGRRLDHIWVSTDLIGNLA
RHAILQDARDWERGSDHVPVVLTLDL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=275, Percent_Identity=32, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI18375503, Length=275, Percent_Identity=32, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI18375501, Length=275, Percent_Identity=32, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788046, Length=271, Percent_Identity=29.520295202952, Blast_Score=109, Evalue=2e-25,
Organism=Drosophila melanogaster, GI221330655, Length=277, Percent_Identity=29.6028880866426, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136678, Length=277, Percent_Identity=29.6028880866426, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30395; Mature: 30264

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALTVATWNINSVRLRRDLLHSLMDQAAPDIVCLQETKVPDDLFPEDLGTALGLPHVLK
CCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHH
RGMKGYNGVAILSRLPLTAVEDTPDWCMKSDCRHIAARIDTGTDPLVLHNFYVPAGGDIP
HHCCCCCCCHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
DRAENPKYGHKLDFIAEARDHFAMNRPHRTMLVGDLNIAPLENDVWSHKQLLNVVSHTPP
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
ETEGLNAWRTEGFIDAIRHFVPDDQKLFTWWSYRNRDWRASNRGRRLDHIWVSTDLIGNL
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEHHHHHHHH
ARHAILQDARDWERGSDHVPVVLTLDL
HHHHHHHHHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure 
TALTVATWNINSVRLRRDLLHSLMDQAAPDIVCLQETKVPDDLFPEDLGTALGLPHVLK
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHH
RGMKGYNGVAILSRLPLTAVEDTPDWCMKSDCRHIAARIDTGTDPLVLHNFYVPAGGDIP
HHCCCCCCCHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
DRAENPKYGHKLDFIAEARDHFAMNRPHRTMLVGDLNIAPLENDVWSHKQLLNVVSHTPP
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
ETEGLNAWRTEGFIDAIRHFVPDDQKLFTWWSYRNRDWRASNRGRRLDHIWVSTDLIGNL
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEHHHHHHHH
ARHAILQDARDWERGSDHVPVVLTLDL
HHHHHHHHHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]