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The map label for this gene is livJ [C]

Identifier: 148259377

GI number: 148259377

Start: 408236

End: 409381

Strand: Reverse

Name: livJ [C]

Synonym: Acry_0360

Alternate gene names: 148259377

Gene position: 409381-408236 (Counterclockwise)

Preceding gene: 148259378

Following gene: 148259376

Centisome position: 12.08

GC content: 65.71

Gene sequence:

>1146_bases
ATGATTCGTAAGATGGGGATCGGGCTCGCGGCGGCCGTTGCTCTGGCGGCGCCGTATGGCATTGCCCACGCGGCCTCCGG
GCCGATCAAGATCGGCGTGCAGGCGCCCATCACCGGCGCCTACGCCGATGAGGGCCAGGGCATCGAGAAGGCCGTGAAGC
TGCTCGCCGACCAGCAGAACGCCAAGGGCGGCCTGCTGGGCCGCAAGATCGAGGTCAAGGTCTGCGACGACGAGGGCAAG
GCCTCCCAGGCCGCCATCTGCGCGCGCCAGCTCGTGAATGACGGCGTGATCGCCGTGATCGGCTCCTACACCAGCGGCGC
GGCACTCGCGGCCGAGCCGATCTATGCGCGCTCGAACGTCATCCAGACCTCGGACGGCACCAGCAACAAGCTGCTCTCGC
GCGGCTACAAGACCTGGTTCGGCAACGCGGCGCCGAACAGCGCGGAGGCGAAGTTCACCGCGAAATACCTGATCGATGTC
CGCCACTTCAAGCGCATCGCCGTGCTGACCGACCATTCGAGCTTCGCCACCGGCCTCGCCGATGCGGTCATCAAGAACAT
CAAGGCCGACCATGGCAAGGTGATCGACAAGGCCTTCATCACCGCCGGCTCGCAGAACTTCACCCCGGTGCTGACCAAGC
TGAAGGCGCTGAAGCCGGATGCGATCTATTTCTCGGGCTACTATTCGGATGGCGGCCTGATCAAGGCGCAGATGGCCCAG
CTCGGCATCACCGCGACCTTCGTCGGCGGCGACGCCAACCAGAACGTCGCCTTCGGCAAGATCGCCGGCTCCGCGGCGAA
GGGCGCCATCATCGTCAACGTGCCCGGTCCGGAACAGCTCCCCTACCCCACCGCCAAGGCCTTCGTCGCAGCCTACAAGG
CGAAATATCACCAGAGCCCGCCCTCGGTCTTCACCCTCACCAACGCCGACGGCATGCGCGCGGTGATGCAGGCGATCGAG
CAGACCAAGAGCGTCAAGCCGGCGAAGCTCGAGCACTACCTGCACACGATGAAGAGCTTCGATGGTCTGACCGGCCAGTT
CCGCTGGAACGACAAGGGCGAGCGCGTCGGCAGCCCGTTCACCGCCTTCGAGGTGCAGGCCGACGGCAGCTACAAGATCG
TCTATCCGGCGCCCAAGGCCGGCTGA

Upstream 100 bases:

>100_bases
GGATTGGCGGCCGACCGGGCGGTCGATCCGTGGCTGGAAGGTTGCCAAGCCGATCCTGTTGCGACAGGCTCGGACCAATC
AATCAAAAAAGGAGATCAGG

Downstream 100 bases:

>100_bases
CGGGCCGGTCGCGCCGGGGGGAGGTTTCGTCATGGGAACGTTCATCCAGCAGGTCTTCAACGGCCTGACGGTCGGGGGCA
TCTACGCGCTCATCGCGCTG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MIRKMGIGLAAAVALAAPYGIAHAASGPIKIGVQAPITGAYADEGQGIEKAVKLLADQQNAKGGLLGRKIEVKVCDDEGK
ASQAAICARQLVNDGVIAVIGSYTSGAALAAEPIYARSNVIQTSDGTSNKLLSRGYKTWFGNAAPNSAEAKFTAKYLIDV
RHFKRIAVLTDHSSFATGLADAVIKNIKADHGKVIDKAFITAGSQNFTPVLTKLKALKPDAIYFSGYYSDGGLIKAQMAQ
LGITATFVGGDANQNVAFGKIAGSAAKGAIIVNVPGPEQLPYPTAKAFVAAYKAKYHQSPPSVFTLTNADGMRAVMQAIE
QTKSVKPAKLEHYLHTMKSFDGLTGQFRWNDKGERVGSPFTAFEVQADGSYKIVYPAPKAG

Sequences:

>Translated_381_residues
MIRKMGIGLAAAVALAAPYGIAHAASGPIKIGVQAPITGAYADEGQGIEKAVKLLADQQNAKGGLLGRKIEVKVCDDEGK
ASQAAICARQLVNDGVIAVIGSYTSGAALAAEPIYARSNVIQTSDGTSNKLLSRGYKTWFGNAAPNSAEAKFTAKYLIDV
RHFKRIAVLTDHSSFATGLADAVIKNIKADHGKVIDKAFITAGSQNFTPVLTKLKALKPDAIYFSGYYSDGGLIKAQMAQ
LGITATFVGGDANQNVAFGKIAGSAAKGAIIVNVPGPEQLPYPTAKAFVAAYKAKYHQSPPSVFTLTNADGMRAVMQAIE
QTKSVKPAKLEHYLHTMKSFDGLTGQFRWNDKGERVGSPFTAFEVQADGSYKIVYPAPKAG
>Mature_381_residues
MIRKMGIGLAAAVALAAPYGIAHAASGPIKIGVQAPITGAYADEGQGIEKAVKLLADQQNAKGGLLGRKIEVKVCDDEGK
ASQAAICARQLVNDGVIAVIGSYTSGAALAAEPIYARSNVIQTSDGTSNKLLSRGYKTWFGNAAPNSAEAKFTAKYLIDV
RHFKRIAVLTDHSSFATGLADAVIKNIKADHGKVIDKAFITAGSQNFTPVLTKLKALKPDAIYFSGYYSDGGLIKAQMAQ
LGITATFVGGDANQNVAFGKIAGSAAKGAIIVNVPGPEQLPYPTAKAFVAAYKAKYHQSPPSVFTLTNADGMRAVMQAIE
QTKSVKPAKLEHYLHTMKSFDGLTGQFRWNDKGERVGSPFTAFEVQADGSYKIVYPAPKAG

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=367, Percent_Identity=30.5177111716621, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789867, Length=376, Percent_Identity=30.0531914893617, Blast_Score=145, Evalue=6e-36,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40086; Mature: 40086

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKMGIGLAAAVALAAPYGIAHAASGPIKIGVQAPITGAYADEGQGIEKAVKLLADQQN
CCCCCCCHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCC
AKGGLLGRKIEVKVCDDEGKASQAAICARQLVNDGVIAVIGSYTSGAALAAEPIYARSNV
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCE
IQTSDGTSNKLLSRGYKTWFGNAAPNSAEAKFTAKYLIDVRHFKRIAVLTDHSSFATGLA
EECCCCCHHHHHHCCCHHHCCCCCCCCCCCEEEHHHHHHHHHCEEEEEEECCCHHHHHHH
DAVIKNIKADHGKVIDKAFITAGSQNFTPVLTKLKALKPDAIYFSGYYSDGGLIKAQMAQ
HHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCEEEEEHHH
LGITATFVGGDANQNVAFGKIAGSAAKGAIIVNVPGPEQLPYPTAKAFVAAYKAKYHQSP
CCEEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCC
PSVFTLTNADGMRAVMQAIEQTKSVKPAKLEHYLHTMKSFDGLTGQFRWNDKGERVGSPF
CCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
TAFEVQADGSYKIVYPAPKAG
EEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure
MIRKMGIGLAAAVALAAPYGIAHAASGPIKIGVQAPITGAYADEGQGIEKAVKLLADQQN
CCCCCCCHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCC
AKGGLLGRKIEVKVCDDEGKASQAAICARQLVNDGVIAVIGSYTSGAALAAEPIYARSNV
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCE
IQTSDGTSNKLLSRGYKTWFGNAAPNSAEAKFTAKYLIDVRHFKRIAVLTDHSSFATGLA
EECCCCCHHHHHHCCCHHHCCCCCCCCCCCEEEHHHHHHHHHCEEEEEEECCCHHHHHHH
DAVIKNIKADHGKVIDKAFITAGSQNFTPVLTKLKALKPDAIYFSGYYSDGGLIKAQMAQ
HHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCEEEEEHHH
LGITATFVGGDANQNVAFGKIAGSAAKGAIIVNVPGPEQLPYPTAKAFVAAYKAKYHQSP
CCEEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCC
PSVFTLTNADGMRAVMQAIEQTKSVKPAKLEHYLHTMKSFDGLTGQFRWNDKGERVGSPF
CCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
TAFEVQADGSYKIVYPAPKAG
EEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]