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The map label for this gene is fadE25 [H]

Identifier: 148259273

GI number: 148259273

Start: 289867

End: 291078

Strand: Reverse

Name: fadE25 [H]

Synonym: Acry_0253

Alternate gene names: 148259273

Gene position: 291078-289867 (Counterclockwise)

Preceding gene: 148259274

Following gene: 148259272

Centisome position: 8.59

GC content: 65.02

Gene sequence:

>1212_bases
ATGGAACTCAGCTTTTCGCCCGAGGAAATCGCGTTCCGCGACGAGGTGCGCGCCTTCATCCGCGACAACCTGCCCGGGGA
TGTGCAGAAGCGCCTGATCGAGGGACGCGAGATCGGCAAGCAGGACATTGTCGACTGGCAGCGCAAGCTGAACGCGAAAG
GCTGGGCAGTGCCGCACTGGCCGCGCGCCTGGGGCGGGCAGGACTGGAGCCCGGTGCAGGAATTCATCCTGCTCGACGAG
TTGCAATCGGCCCCCGCGCCCAGCCCGCTGCCGTTCAACGTCTCCATGGTCGGCCCGGTGATCGCGACCTTCGGCAATGA
CGAGCAGAAGCGGAAATTCCTGCCGGCGACGGCCAACCTCGATATCTGGTGGTGCCAGGGCTTCTCCGAGCCGGGGGCGG
GATCGGATCTTGCCGGCCTCAAGACCACGGCCCGCCGCGAGGGCGATCACTACATCATCAACGGCCAGAAGACCTGGACG
ACCCTTGCCCAGTATGCCGACTGGATCTTCGTCCTCGCCCGCACCGATCCGGGTGCGGAGAAGAAGCAGCAAGGCATTTC
CTTCATCCTTGTCGACATGAAGACGCCCGGCATCACCGTCCGGCCGATCCAGCTGATCGATGGCGGGCACGAGGTCAACG
AGGTCTGGTTCGACGAGGTGAAGGTGCCGGCGGAAAACCTCGTCGGCGAGGAAAACAAGGGCTGGGACTACGCGAAGTTC
CTGCTCGGCAACGAGCGCACCGGTATCGCCCGCGTCGGCGCCTCGAAGCAGCGCCTGCGTCGCATCCGCGAACTCGCCAC
GATCGAGCGCGCCGGCGATGCCAGGCTGATCGACGCGCCCTGGTTCCGCGAGAAGCTCGCGGATGTCGAGATCGATCTCA
AGGCGCTGGAAATCACCCAGCTCCGCGTGCTCGCCGAGGCGCGCAAATCGGGCAAGCATGGCCGGCCGGATCCGAAATCC
TCGATCCTCAAGATCAAGGGTTCGGAAATCCAGCAGGCGACGACCGAGCTTCTGCTCGATGTCGTCGGCCCGCTCGCCAT
GCCGTTCCAGGGCTCGACCGATCCGGATGACGATGGCAACGCCATGCCGATCGGGCCGGACGACGCGGCCGAGGCGGCGC
CCACCTACTTCAACTGGCGGAAGATCTCGATCTACGGCGGCTCGAACGAGATCCAGCGCAACATCATCGCCAAGGCGATC
CTGGGGTTCTGA

Upstream 100 bases:

>100_bases
CCGCCCACCATCGCCGTCGCCCCGCGCGGGCGGATGGACACCCCCACCGGCACCCGCAAGGACAGCGCCGGCCGTTTCGA
GAACAACTGAGGAGAGCGCC

Downstream 100 bases:

>100_bases
GGACAAGACAATGGATTTCGATTTTACCGAAGAACAGCGCCTGCTGCGCGACAGTGTCGGCAAGCTTTTTGCCAGCCGCT
ATGCCGATTTCGAAAAGCGC

Product: acyl-CoA dehydrogenase domain-containing protein

Products: 6-carboxyhex-2-enoyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MELSFSPEEIAFRDEVRAFIRDNLPGDVQKRLIEGREIGKQDIVDWQRKLNAKGWAVPHWPRAWGGQDWSPVQEFILLDE
LQSAPAPSPLPFNVSMVGPVIATFGNDEQKRKFLPATANLDIWWCQGFSEPGAGSDLAGLKTTARREGDHYIINGQKTWT
TLAQYADWIFVLARTDPGAEKKQQGISFILVDMKTPGITVRPIQLIDGGHEVNEVWFDEVKVPAENLVGEENKGWDYAKF
LLGNERTGIARVGASKQRLRRIRELATIERAGDARLIDAPWFREKLADVEIDLKALEITQLRVLAEARKSGKHGRPDPKS
SILKIKGSEIQQATTELLLDVVGPLAMPFQGSTDPDDDGNAMPIGPDDAAEAAPTYFNWRKISIYGGSNEIQRNIIAKAI
LGF

Sequences:

>Translated_403_residues
MELSFSPEEIAFRDEVRAFIRDNLPGDVQKRLIEGREIGKQDIVDWQRKLNAKGWAVPHWPRAWGGQDWSPVQEFILLDE
LQSAPAPSPLPFNVSMVGPVIATFGNDEQKRKFLPATANLDIWWCQGFSEPGAGSDLAGLKTTARREGDHYIINGQKTWT
TLAQYADWIFVLARTDPGAEKKQQGISFILVDMKTPGITVRPIQLIDGGHEVNEVWFDEVKVPAENLVGEENKGWDYAKF
LLGNERTGIARVGASKQRLRRIRELATIERAGDARLIDAPWFREKLADVEIDLKALEITQLRVLAEARKSGKHGRPDPKS
SILKIKGSEIQQATTELLLDVVGPLAMPFQGSTDPDDDGNAMPIGPDDAAEAAPTYFNWRKISIYGGSNEIQRNIIAKAI
LGF
>Mature_403_residues
MELSFSPEEIAFRDEVRAFIRDNLPGDVQKRLIEGREIGKQDIVDWQRKLNAKGWAVPHWPRAWGGQDWSPVQEFILLDE
LQSAPAPSPLPFNVSMVGPVIATFGNDEQKRKFLPATANLDIWWCQGFSEPGAGSDLAGLKTTARREGDHYIINGQKTWT
TLAQYADWIFVLARTDPGAEKKQQGISFILVDMKTPGITVRPIQLIDGGHEVNEVWFDEVKVPAENLVGEENKGWDYAKF
LLGNERTGIARVGASKQRLRRIRELATIERAGDARLIDAPWFREKLADVEIDLKALEITQLRVLAEARKSGKHGRPDPKS
SILKIKGSEIQQATTELLLDVVGPLAMPFQGSTDPDDDGNAMPIGPDDAAEAAPTYFNWRKISIYGGSNEIQRNIIAKAI
LGF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501857, Length=414, Percent_Identity=26.3285024154589, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI4501859, Length=410, Percent_Identity=28.0487804878049, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4557233, Length=362, Percent_Identity=28.7292817679558, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI7656849, Length=261, Percent_Identity=28.735632183908, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI4557231, Length=352, Percent_Identity=26.9886363636364, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI187960098, Length=352, Percent_Identity=26.9886363636364, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI226958412, Length=234, Percent_Identity=28.2051282051282, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI226958414, Length=178, Percent_Identity=31.4606741573034, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4503943, Length=415, Percent_Identity=25.7831325301205, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI4557235, Length=254, Percent_Identity=29.5275590551181, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI76496475, Length=254, Percent_Identity=29.5275590551181, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI7669494, Length=322, Percent_Identity=27.639751552795, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI38505218, Length=239, Percent_Identity=30.1255230125523, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI21361497, Length=252, Percent_Identity=28.968253968254, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI48976061, Length=217, Percent_Identity=30.4147465437788, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI211971008, Length=217, Percent_Identity=30.4147465437788, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI87081958, Length=416, Percent_Identity=26.2019230769231, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786223, Length=245, Percent_Identity=26.530612244898, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17570075, Length=417, Percent_Identity=27.5779376498801, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17569725, Length=367, Percent_Identity=28.0653950953678, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17506239, Length=411, Percent_Identity=26.7639902676399, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17508101, Length=349, Percent_Identity=26.9340974212034, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17534899, Length=362, Percent_Identity=26.7955801104972, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538396, Length=351, Percent_Identity=26.4957264957265, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32563615, Length=196, Percent_Identity=33.6734693877551, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990804, Length=270, Percent_Identity=28.1481481481481, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17551932, Length=170, Percent_Identity=32.3529411764706, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982178, Length=226, Percent_Identity=29.646017699115, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17505929, Length=348, Percent_Identity=26.1494252873563, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71985192, Length=203, Percent_Identity=32.512315270936, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71985184, Length=203, Percent_Identity=32.512315270936, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17533517, Length=255, Percent_Identity=25.0980392156863, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17562446, Length=240, Percent_Identity=28.75, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17531909, Length=152, Percent_Identity=30.2631578947368, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24666513, Length=347, Percent_Identity=29.1066282420749, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356377, Length=364, Percent_Identity=26.6483516483516, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21355753, Length=321, Percent_Identity=27.7258566978193, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24660351, Length=185, Percent_Identity=32.972972972973, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI281363737, Length=206, Percent_Identity=30.5825242718447, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19920834, Length=420, Percent_Identity=26.6666666666667, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646207, Length=361, Percent_Identity=26.0387811634349, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.1.62

Molecular weight: Translated: 44930; Mature: 44930

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSFSPEEIAFRDEVRAFIRDNLPGDVQKRLIEGREIGKQDIVDWQRKLNAKGWAVPHW
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
PRAWGGQDWSPVQEFILLDELQSAPAPSPLPFNVSMVGPVIATFGNDEQKRKFLPATANL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCC
DIWWCQGFSEPGAGSDLAGLKTTARREGDHYIINGQKTWTTLAQYADWIFVLARTDPGAE
EEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHCCEEEEEEECCCCHH
KKQQGISFILVDMKTPGITVRPIQLIDGGHEVNEVWFDEVKVPAENLVGEENKGWDYAKF
HHHCCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCHHHHCCCCCCCCHHHHH
LLGNERTGIARVGASKQRLRRIRELATIERAGDARLIDAPWFREKLADVEIDLKALEITQ
EECCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHCCCEEEEEHHHHHH
LRVLAEARKSGKHGRPDPKSSILKIKGSEIQQATTELLLDVVGPLAMPFQGSTDPDDDGN
HHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AMPIGPDDAAEAAPTYFNWRKISIYGGSNEIQRNIIAKAILGF
CCCCCCCCHHHCCCCEECEEEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELSFSPEEIAFRDEVRAFIRDNLPGDVQKRLIEGREIGKQDIVDWQRKLNAKGWAVPHW
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
PRAWGGQDWSPVQEFILLDELQSAPAPSPLPFNVSMVGPVIATFGNDEQKRKFLPATANL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCC
DIWWCQGFSEPGAGSDLAGLKTTARREGDHYIINGQKTWTTLAQYADWIFVLARTDPGAE
EEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHCCEEEEEEECCCCHH
KKQQGISFILVDMKTPGITVRPIQLIDGGHEVNEVWFDEVKVPAENLVGEENKGWDYAKF
HHHCCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCHHHHCCCCCCCCHHHHH
LLGNERTGIARVGASKQRLRRIRELATIERAGDARLIDAPWFREKLADVEIDLKALEITQ
EECCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHCCCEEEEEHHHHHH
LRVLAEARKSGKHGRPDPKSSILKIKGSEIQQATTELLLDVVGPLAMPFQGSTDPDDDGN
HHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AMPIGPDDAAEAAPTYFNWRKISIYGGSNEIQRNIIAKAILGF
CCCCCCCCHHHCCCCEECEEEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pimeloyl-CoA; NAD+

Specific reaction: pimeloyl-CoA + NAD+ = 6-carboxyhex-2-enoyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]