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The map label for this gene is dacA [H]

Identifier: 148259268

GI number: 148259268

Start: 284993

End: 286264

Strand: Reverse

Name: dacA [H]

Synonym: Acry_0248

Alternate gene names: 148259268

Gene position: 286264-284993 (Counterclockwise)

Preceding gene: 148259272

Following gene: 148259267

Centisome position: 8.45

GC content: 68.47

Gene sequence:

>1272_bases
ATGCCTCTCGCCATGCCAACAGTCCGGACTTCCGCCACCATGAAGCGCCGCCTTTCACTTCTCACCCTCGCCCTGTTCAC
CGCTACGTCCTACGCCGCCGGGCGCACGCCCGCGCCGGGCAAGGGCGCGGCACACGCAACCGCCGCGGCGACAACCGCGC
CGCAGGACGCGGGACCCGCGCCAGTGGCGCCCCTCGCCATTCCCGGCGTGCCGATGCCGCCGACCCTGCCGCAGCAGGCC
TCCTATGTGCTGATGGACGCCGATACCGGCGCGGTGATCGCCGCCAAGGCGCCGGACCAGCCCTGGCCACCGGCCAGCCT
GACCAAGCTGATGACCGCCTATCTGACCTACCAGGCTGTCGCGCACGGTTCGCTGAAGATGGACCAGACCGTGCCGGTCA
GCGTCTCGGCCTGGCATACCGGCGGCTCGCGCATGTTCATCTCGCCGGGCATGACGGTCACGGTGGACCAGTTGCTACAC
GGGCTGATCATCGACTCGGGCAACGATGCGGCCGTGGCGCTCGCCCAGGCGGTGGCCGGCAGCCGGAGCGGCTTCGTCGG
GCTGATGAACCATGAAGCCAAGGCGCTTGGCCTGAAGTCGACCCACTACACGAACGTGGACGGCCTGCCGAACCCGGACC
TGCGCACCACGGCGATGGATGTTGCAAAACTGTCGCGCGACATCGTCCGTACCTATCCGCAGTTCCTGAAGATTTCGGTC
AAGAAGCACTTCACCTTCGACAAGATCCGCCAGCGAAGCTGGAACCCGGTGCTGTTCCACGACCCGACGGTGGATGGGCT
TAAGACCGGCCGCACCAAGGAAGCCGGGCACTGCATCGACGCCACAGCACTGCGCAACGGCCGCCGCCTCATCGCCGTGG
TGCTCGGCGGGCCGAGCTGGACCGTCAGCACGCACGATATCGAGGCCCTGCTCGATTACGGCTACCAGTTCTACGCCAAT
GCCAAGGTCGCGGTTGCCGGCAAGCCGGCGGGCACGCTGAAGGCGCCGCTCTACCGCACGACCGCGGTGGCGGTCGGCCC
GGCGCACAGCATCACGATGACGATCCCGGCGCTTGCCGCGAAGACCCTGAAGACCTCCGTGGCGCTGGACCCGCCGCCGG
CAGCCGGCGTGCATCGCGGGCAGACGGTCGGCACGATCACCGTCAGCGCCGGCGGCAAGACCCTGGCGACCGTTCCCGCC
GTCGCCCTCGCGGACGACCCGCCGGCCGGATTCGTTACAAGGATGATGAGGAAGCTGAAAGCCGCGCTCTGA

Upstream 100 bases:

>100_bases
TCAAACCGTGCGGGGGGCGCGAAGTCAAGGAAGTGCGGCGAAACACCACCGTGAACGCGACGCACGGCCTCGCGATTTGC
CACGGCACGGGGGCGATTGT

Downstream 100 bases:

>100_bases
CGGCGACGCTCCGATACAGGCCGCGCCCTGACGGGCGCGGCGCCTCTCAGCCGATCAGGACTGTCGGCATGCCGAGAATG
ATGGTGCCGCCGTGCGCGGT

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MPLAMPTVRTSATMKRRLSLLTLALFTATSYAAGRTPAPGKGAAHATAAATTAPQDAGPAPVAPLAIPGVPMPPTLPQQA
SYVLMDADTGAVIAAKAPDQPWPPASLTKLMTAYLTYQAVAHGSLKMDQTVPVSVSAWHTGGSRMFISPGMTVTVDQLLH
GLIIDSGNDAAVALAQAVAGSRSGFVGLMNHEAKALGLKSTHYTNVDGLPNPDLRTTAMDVAKLSRDIVRTYPQFLKISV
KKHFTFDKIRQRSWNPVLFHDPTVDGLKTGRTKEAGHCIDATALRNGRRLIAVVLGGPSWTVSTHDIEALLDYGYQFYAN
AKVAVAGKPAGTLKAPLYRTTAVAVGPAHSITMTIPALAAKTLKTSVALDPPPAAGVHRGQTVGTITVSAGGKTLATVPA
VALADDPPAGFVTRMMRKLKAAL

Sequences:

>Translated_423_residues
MPLAMPTVRTSATMKRRLSLLTLALFTATSYAAGRTPAPGKGAAHATAAATTAPQDAGPAPVAPLAIPGVPMPPTLPQQA
SYVLMDADTGAVIAAKAPDQPWPPASLTKLMTAYLTYQAVAHGSLKMDQTVPVSVSAWHTGGSRMFISPGMTVTVDQLLH
GLIIDSGNDAAVALAQAVAGSRSGFVGLMNHEAKALGLKSTHYTNVDGLPNPDLRTTAMDVAKLSRDIVRTYPQFLKISV
KKHFTFDKIRQRSWNPVLFHDPTVDGLKTGRTKEAGHCIDATALRNGRRLIAVVLGGPSWTVSTHDIEALLDYGYQFYAN
AKVAVAGKPAGTLKAPLYRTTAVAVGPAHSITMTIPALAAKTLKTSVALDPPPAAGVHRGQTVGTITVSAGGKTLATVPA
VALADDPPAGFVTRMMRKLKAAL
>Mature_422_residues
PLAMPTVRTSATMKRRLSLLTLALFTATSYAAGRTPAPGKGAAHATAAATTAPQDAGPAPVAPLAIPGVPMPPTLPQQAS
YVLMDADTGAVIAAKAPDQPWPPASLTKLMTAYLTYQAVAHGSLKMDQTVPVSVSAWHTGGSRMFISPGMTVTVDQLLHG
LIIDSGNDAAVALAQAVAGSRSGFVGLMNHEAKALGLKSTHYTNVDGLPNPDLRTTAMDVAKLSRDIVRTYPQFLKISVK
KHFTFDKIRQRSWNPVLFHDPTVDGLKTGRTKEAGHCIDATALRNGRRLIAVVLGGPSWTVSTHDIEALLDYGYQFYANA
KVAVAGKPAGTLKAPLYRTTAVAVGPAHSITMTIPALAAKTLKTSVALDPPPAAGVHRGQTVGTITVSAGGKTLATVPAV
ALADDPPAGFVTRMMRKLKAAL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=362, Percent_Identity=36.1878453038674, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1787062, Length=346, Percent_Identity=37.5722543352601, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87082022, Length=358, Percent_Identity=36.3128491620112, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87082054, Length=223, Percent_Identity=28.2511210762332, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44123; Mature: 43991

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLAMPTVRTSATMKRRLSLLTLALFTATSYAAGRTPAPGKGAAHATAAATTAPQDAGPA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCC
PVAPLAIPGVPMPPTLPQQASYVLMDADTGAVIAAKAPDQPWPPASLTKLMTAYLTYQAV
CCCCEECCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
AHGSLKMDQTVPVSVSAWHTGGSRMFISPGMTVTVDQLLHGLIIDSGNDAAVALAQAVAG
HCCCEEECCCCCEEEEEEECCCCEEEECCCCEEEHHHHHHEEEEECCCCHHHHHHHHHHC
SRSGFVGLMNHEAKALGLKSTHYTNVDGLPNPDLRTTAMDVAKLSRDIVRTYPQFLKISV
CCCCEEEEECCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHEEEH
KKHFTFDKIRQRSWNPVLFHDPTVDGLKTGRTKEAGHCIDATALRNGRRLIAVVLGGPSW
HHCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCEEEHHHHCCCCEEEEEEECCCCE
TVSTHDIEALLDYGYQFYANAKVAVAGKPAGTLKAPLYRTTAVAVGPAHSITMTIPALAA
EEEHHHHHHHHHCCEEEEECCEEEEECCCCCCCCCCCEEEEEEEECCCCEEEEEHHHHHH
KTLKTSVALDPPPAAGVHRGQTVGTITVSAGGKTLATVPAVALADDPPAGFVTRMMRKLK
HHHHHEEEECCCCCCCCCCCCEEEEEEEECCCCEEEECCEEEECCCCCHHHHHHHHHHHH
AAL
HCC
>Mature Secondary Structure 
PLAMPTVRTSATMKRRLSLLTLALFTATSYAAGRTPAPGKGAAHATAAATTAPQDAGPA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCC
PVAPLAIPGVPMPPTLPQQASYVLMDADTGAVIAAKAPDQPWPPASLTKLMTAYLTYQAV
CCCCEECCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
AHGSLKMDQTVPVSVSAWHTGGSRMFISPGMTVTVDQLLHGLIIDSGNDAAVALAQAVAG
HCCCEEECCCCCEEEEEEECCCCEEEECCCCEEEHHHHHHEEEEECCCCHHHHHHHHHHC
SRSGFVGLMNHEAKALGLKSTHYTNVDGLPNPDLRTTAMDVAKLSRDIVRTYPQFLKISV
CCCCEEEEECCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHEEEH
KKHFTFDKIRQRSWNPVLFHDPTVDGLKTGRTKEAGHCIDATALRNGRRLIAVVLGGPSW
HHCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCEEEHHHHCCCCEEEEEEECCCCE
TVSTHDIEALLDYGYQFYANAKVAVAGKPAGTLKAPLYRTTAVAVGPAHSITMTIPALAA
EEEHHHHHHHHHCCEEEEECCEEEEECCCCCCCCCCCEEEEEEEECCCCEEEEEHHHHHH
KTLKTSVALDPPPAAGVHRGQTVGTITVSAGGKTLATVPAVALADDPPAGFVTRMMRKLK
HHHHHEEEECCCCCCCCCCCCEEEEEEEECCCCEEEECCEEEECCCCCHHHHHHHHHHHH
AAL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]