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The map label for this gene is hemB [H]

Identifier: 148259201

GI number: 148259201

Start: 205407

End: 206402

Strand: Reverse

Name: hemB [H]

Synonym: Acry_0181

Alternate gene names: 148259201

Gene position: 206402-205407 (Counterclockwise)

Preceding gene: 148259202

Following gene: 148259198

Centisome position: 6.09

GC content: 68.78

Gene sequence:

>996_bases
ATGATCACCGGCAGCTTTCCGACCACCCGCCTGCGCCGCAACCGCCGCGATGGCTGGACGCGGCGCATGGTCGCCGAGAC
CACGCTGTCGGTGGACAATCTGATCTGGCCGATCTTCGTCCGCGCCGGCGATGGCGAGCCGACGCCGGTCGCCACCATGC
CGGGTGTCGTGCGGGTCGGGCTCGACCGCCTCGCCGCCCATGTCGAGCCGGCGGCGCGGCTCGGTATTCCGGCCATCGCC
CTGTTTCCCGCCACCCCGCCCGAGCTCAAGAACCCCGAGGGCACCGAGGCGCTGAACCCGGACAACCTGATCTGCCAGGC
CGCCCGGCTGCTCAAGCGCGAATTCCCCGAGCTCGGCCTGATCGGCGATGTGGCGCTCGATCCCTATACCGATCACGGGC
ATGACGGCGTGCTGCGCGAAGGCTATGTCGCCAACGACGAATCGGTCGAGATCCTCGAACGCCAGGCGGTGAACCAGGCG
CTCGCCGGGATCGACGTGATCGCGCCCTCGGACATGATGGACGGGCGGATCGGCAGCATCCGCGCCGCCCTCGATTCCGA
GGGGCTGATCGACACGCGGATCATGAGCTACGCCGCCAAATACGCCTCCGCCTTCTACGGCCCGTTCCGCGAGGCGCTCG
GCTCCAACGCGGCGCTGAAGGGCGACAAGAAGACCTACCAAATGGACCCCGCCAACACCGACGAGGCGCTGCGCGAGGTG
GCGATGGACATCGACGAGGGCGCCGACATGGTGATGGTCAAGCCGGGCATGCCCTATCTCGACATCATCCGCCGCGTGCA
CGAGCGCTTCGCGGTGCCGACCTTCGCCTATCAGGTGAGCGGCGAATACGCGATGCTCGCCACCGCGATGGAACACGGGC
TGCTCGACCGCGAGCGCGGCATTCTCGAAACGCTGATGGGCTTCCGCCGCGCCGGCGCCGCCGGGGTGCTCACCTATTTC
GCGGTGGAAGCGGCAACCCTGCTGCGCCGGGGCTGA

Upstream 100 bases:

>100_bases
CGAGGGCGGCAACGCATAGGCGCAGCGCAGCCTGCGCCCCTTGCGCCGGCCCCTTCCATGCGCAATTCGACTAGGTGTGT
AACGAGCACGAGGAGCAGCA

Downstream 100 bases:

>100_bases
GCCGGCGATCACAGGTCGGGCGGCGCCAGGTGCGCGCCGCCCCCTCAACTCACATGCCGAGCGCGCGGCGATAGACGTCG
ATCAGCATTTCCTGCTCCTC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MITGSFPTTRLRRNRRDGWTRRMVAETTLSVDNLIWPIFVRAGDGEPTPVATMPGVVRVGLDRLAAHVEPAARLGIPAIA
LFPATPPELKNPEGTEALNPDNLICQAARLLKREFPELGLIGDVALDPYTDHGHDGVLREGYVANDESVEILERQAVNQA
LAGIDVIAPSDMMDGRIGSIRAALDSEGLIDTRIMSYAAKYASAFYGPFREALGSNAALKGDKKTYQMDPANTDEALREV
AMDIDEGADMVMVKPGMPYLDIIRRVHERFAVPTFAYQVSGEYAMLATAMEHGLLDRERGILETLMGFRRAGAAGVLTYF
AVEAATLLRRG

Sequences:

>Translated_331_residues
MITGSFPTTRLRRNRRDGWTRRMVAETTLSVDNLIWPIFVRAGDGEPTPVATMPGVVRVGLDRLAAHVEPAARLGIPAIA
LFPATPPELKNPEGTEALNPDNLICQAARLLKREFPELGLIGDVALDPYTDHGHDGVLREGYVANDESVEILERQAVNQA
LAGIDVIAPSDMMDGRIGSIRAALDSEGLIDTRIMSYAAKYASAFYGPFREALGSNAALKGDKKTYQMDPANTDEALREV
AMDIDEGADMVMVKPGMPYLDIIRRVHERFAVPTFAYQVSGEYAMLATAMEHGLLDRERGILETLMGFRRAGAAGVLTYF
AVEAATLLRRG
>Mature_331_residues
MITGSFPTTRLRRNRRDGWTRRMVAETTLSVDNLIWPIFVRAGDGEPTPVATMPGVVRVGLDRLAAHVEPAARLGIPAIA
LFPATPPELKNPEGTEALNPDNLICQAARLLKREFPELGLIGDVALDPYTDHGHDGVLREGYVANDESVEILERQAVNQA
LAGIDVIAPSDMMDGRIGSIRAALDSEGLIDTRIMSYAAKYASAFYGPFREALGSNAALKGDKKTYQMDPANTDEALREV
AMDIDEGADMVMVKPGMPYLDIIRRVHERFAVPTFAYQVSGEYAMLATAMEHGLLDRERGILETLMGFRRAGAAGVLTYF
AVEAATLLRRG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=42.8571428571429, Blast_Score=234, Evalue=7e-62,
Organism=Escherichia coli, GI87081728, Length=316, Percent_Identity=46.5189873417722, Blast_Score=259, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6321398, Length=308, Percent_Identity=40.9090909090909, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI21358291, Length=304, Percent_Identity=40.1315789473684, Blast_Score=241, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36115; Mature: 36115

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGSFPTTRLRRNRRDGWTRRMVAETTLSVDNLIWPIFVRAGDGEPTPVATMPGVVRVG
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCHHHHHH
LDRLAAHVEPAARLGIPAIALFPATPPELKNPEGTEALNPDNLICQAARLLKREFPELGL
HHHHHHHCCHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
IGDVALDPYTDHGHDGVLREGYVANDESVEILERQAVNQALAGIDVIAPSDMMDGRIGSI
EECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCHHHCCCHHHH
RAALDSEGLIDTRIMSYAAKYASAFYGPFREALGSNAALKGDKKTYQMDPANTDEALREV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCHHHHHHHH
AMDIDEGADMVMVKPGMPYLDIIRRVHERFAVPTFAYQVSGEYAMLATAMEHGLLDRERG
HHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHH
ILETLMGFRRAGAAGVLTYFAVEAATLLRRG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITGSFPTTRLRRNRRDGWTRRMVAETTLSVDNLIWPIFVRAGDGEPTPVATMPGVVRVG
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCHHHHHH
LDRLAAHVEPAARLGIPAIALFPATPPELKNPEGTEALNPDNLICQAARLLKREFPELGL
HHHHHHHCCHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
IGDVALDPYTDHGHDGVLREGYVANDESVEILERQAVNQALAGIDVIAPSDMMDGRIGSI
EECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCHHHCCCHHHH
RAALDSEGLIDTRIMSYAAKYASAFYGPFREALGSNAALKGDKKTYQMDPANTDEALREV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCHHHHHHHH
AMDIDEGADMVMVKPGMPYLDIIRRVHERFAVPTFAYQVSGEYAMLATAMEHGLLDRERG
HHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHH
ILETLMGFRRAGAAGVLTYFAVEAATLLRRG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]