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The map label for this gene is pyk [H]

Identifier: 148259198

GI number: 148259198

Start: 203141

End: 204565

Strand: Reverse

Name: pyk [H]

Synonym: Acry_0178

Alternate gene names: 148259198

Gene position: 204565-203141 (Counterclockwise)

Preceding gene: 148259201

Following gene: 148259197

Centisome position: 6.04

GC content: 70.25

Gene sequence:

>1425_bases
ATGGCATCCAGGCTGCGGCGTCATCGGCGCACGAAAATCGTTGCAACCATCGGGCCGGCAAGTTCGACGCCGGAGATACT
GTCGCAGCTCTTCGAGACCGGCGCCGACGTGTTCCGGCTCAATTTCAGCCACGGCACGCATGAGGATCACCTTGCGCGCC
TGCAGATGATCCGCGCGCTGGAGCGGAAGTTCGACCGGCCGATCGGCATCCTCGCCGACGTGCAGGGGCCGAAGCTGCGC
GTCGGCCAGTTTCGCGGCGGACGCGTGCAGTTGCAGACCGGCGCCGAATTCCAGCTCGACCTCAACCCCGCCCCCGGCGA
TACCCGCCGCGTCAACCTGCCGCATCCCGAGATCATCGAGGCCGCCAGCATCGGCGCCACCCTGCTGCTCGATGACGGCA
AGCTGCGCCTGCGGGTGCTGCGCAAGCGCGAGGACCACCTGCTGACCGAGGTCGTCACCGGCGGCCCGCTCTCCGACCGC
AAGGGCGTCAACGTGCCCGACGTGGTGCTGCCGATCCCGGCGCTGACGAAGAAGGACCGCGCCGATCTCGCCTTCGCGCT
CGAACACGGCATCGACTATATCGGCCTGTCCTTCGTCCAGCGCGCCGCCGACGTGCTGGAGGCGAAGGAGATCGCCGCCG
GCCGCGCCCTGGTGATGACCAAGATGGAAAAGCCCCAGGCGCTCGACAATCTCGACGAGATCATGGACGCGACGGATGCC
GTCATGGTCGCGCGCGGCGATCTCGGCGTCGAGCTGCCGCCCGAGGAAGTGCCGATCGTGCAGAAGCGCATCATCCGCGC
CGCCCAGCAGCGCGGCCTGCCGGTGGTCGTCGCGACGCAGATGCTGGAGAGCATGATCTCCGCCCCCGCCCCCACCCGCG
CCGAGGCATCGGACGTGGCGACCGCGGTGTTCGACGGCGCCGATGCGGTGATGCTCTCGGCCGAGAGTGCCGCCGGCCAG
TATCCGCGCGAGGCGGTGAACATGATGGACCGCATCGTCGCCCGGGTGGAGCGGGACGATGACTGGCGGCGGACGATCGA
CGCCAAGCGGCCGGACCCCGAACCTTCCTCGGCCGATGCCATCGCCCGTGCCGCCGAACAGGTCGCGCACACGATCGGCG
CGCGCGCCATCGTCGCCTTCACCAGTTCCGGCGCCAGCGCGCTGCGCGTCGCCCGCGAGCGGCCGGACTGCCCCGTGATC
GGCCTGACACCCAGTCTGGACACCGCCCGCCGCCTCGCGGCGGTCTGGGGCGTGCATGCCGTGGTCACGCAGGACGTGGG
CTCGATGACCGAGGCTGTCGCCCTTTCGGCGAAGGTCGCGCGGCAGGAGGGCTTCGCCCAGCCGGGCGAAGAAATCGTCG
TCGTCGCCGGCGTCCCCTTCGGCCAGAGCGGCACGACCAACGCGCTGCGCGTGGCCAGGGTGTAA

Upstream 100 bases:

>100_bases
ATGTTTGCCATAGAGACGTTTATCACGACGCCGGTCACGGCGCGACACGATAACCCGGTCATCGGGACCTGGCAGGCGGA
CAGATTCAGAGGGGACCAGC

Downstream 100 bases:

>100_bases
CCCGCACGCCCCTTGCATTGCCGCCGTGCCCGGGCAAGACTGAAGCCTAGGGAGGACATCATATGATCGCGATTACCGTT
CTGTATCCGAAGACCGCCAC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MASRLRRHRRTKIVATIGPASSTPEILSQLFETGADVFRLNFSHGTHEDHLARLQMIRALERKFDRPIGILADVQGPKLR
VGQFRGGRVQLQTGAEFQLDLNPAPGDTRRVNLPHPEIIEAASIGATLLLDDGKLRLRVLRKREDHLLTEVVTGGPLSDR
KGVNVPDVVLPIPALTKKDRADLAFALEHGIDYIGLSFVQRAADVLEAKEIAAGRALVMTKMEKPQALDNLDEIMDATDA
VMVARGDLGVELPPEEVPIVQKRIIRAAQQRGLPVVVATQMLESMISAPAPTRAEASDVATAVFDGADAVMLSAESAAGQ
YPREAVNMMDRIVARVERDDDWRRTIDAKRPDPEPSSADAIARAAEQVAHTIGARAIVAFTSSGASALRVARERPDCPVI
GLTPSLDTARRLAAVWGVHAVVTQDVGSMTEAVALSAKVARQEGFAQPGEEIVVVAGVPFGQSGTTNALRVARV

Sequences:

>Translated_474_residues
MASRLRRHRRTKIVATIGPASSTPEILSQLFETGADVFRLNFSHGTHEDHLARLQMIRALERKFDRPIGILADVQGPKLR
VGQFRGGRVQLQTGAEFQLDLNPAPGDTRRVNLPHPEIIEAASIGATLLLDDGKLRLRVLRKREDHLLTEVVTGGPLSDR
KGVNVPDVVLPIPALTKKDRADLAFALEHGIDYIGLSFVQRAADVLEAKEIAAGRALVMTKMEKPQALDNLDEIMDATDA
VMVARGDLGVELPPEEVPIVQKRIIRAAQQRGLPVVVATQMLESMISAPAPTRAEASDVATAVFDGADAVMLSAESAAGQ
YPREAVNMMDRIVARVERDDDWRRTIDAKRPDPEPSSADAIARAAEQVAHTIGARAIVAFTSSGASALRVARERPDCPVI
GLTPSLDTARRLAAVWGVHAVVTQDVGSMTEAVALSAKVARQEGFAQPGEEIVVVAGVPFGQSGTTNALRVARV
>Mature_473_residues
ASRLRRHRRTKIVATIGPASSTPEILSQLFETGADVFRLNFSHGTHEDHLARLQMIRALERKFDRPIGILADVQGPKLRV
GQFRGGRVQLQTGAEFQLDLNPAPGDTRRVNLPHPEIIEAASIGATLLLDDGKLRLRVLRKREDHLLTEVVTGGPLSDRK
GVNVPDVVLPIPALTKKDRADLAFALEHGIDYIGLSFVQRAADVLEAKEIAAGRALVMTKMEKPQALDNLDEIMDATDAV
MVARGDLGVELPPEEVPIVQKRIIRAAQQRGLPVVVATQMLESMISAPAPTRAEASDVATAVFDGADAVMLSAESAAGQY
PREAVNMMDRIVARVERDDDWRRTIDAKRPDPEPSSADAIARAAEQVAHTIGARAIVAFTSSGASALRVARERPDCPVIG
LTPSLDTARRLAAVWGVHAVVTQDVGSMTEAVALSAKVARQEGFAQPGEEIVVVAGVPFGQSGTTNALRVARV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=492, Percent_Identity=37.8048780487805, Blast_Score=315, Evalue=8e-86,
Organism=Homo sapiens, GI33286420, Length=492, Percent_Identity=37.8048780487805, Blast_Score=315, Evalue=8e-86,
Organism=Homo sapiens, GI33286418, Length=496, Percent_Identity=37.7016129032258, Blast_Score=311, Evalue=8e-85,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=37.2708757637475, Blast_Score=293, Evalue=4e-79,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=37.2708757637475, Blast_Score=292, Evalue=6e-79,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=36.9337979094077, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=36.9337979094077, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=34.468085106383, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=34.468085106383, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=32.7102803738318, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=38.2165605095541, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=39.9168399168399, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=34.6311475409836, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=37.037037037037, Blast_Score=278, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=37.037037037037, Blast_Score=278, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=37.037037037037, Blast_Score=277, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=37.037037037037, Blast_Score=276, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6319279, Length=490, Percent_Identity=34.8979591836735, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6324923, Length=488, Percent_Identity=34.4262295081967, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=38.0368098159509, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=38.0368098159509, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24581235, Length=492, Percent_Identity=33.5365853658537, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24648966, Length=415, Percent_Identity=35.9036144578313, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24646914, Length=290, Percent_Identity=35.1724137931034, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51042; Mature: 50911

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRLRRHRRTKIVATIGPASSTPEILSQLFETGADVFRLNFSHGTHEDHLARLQMIRAL
CCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
ERKFDRPIGILADVQGPKLRVGQFRGGRVQLQTGAEFQLDLNPAPGDTRRVNLPHPEIIE
HHHHCCCCEEEEECCCCCEEEECCCCCEEEEECCCEEEEECCCCCCCCEEECCCCHHHHH
AASIGATLLLDDGKLRLRVLRKREDHLLTEVVTGGPLSDRKGVNVPDVVLPIPALTKKDR
HHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCH
ADLAFALEHGIDYIGLSFVQRAADVLEAKEIAAGRALVMTKMEKPQALDNLDEIMDATDA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCHHCE
VMVARGDLGVELPPEEVPIVQKRIIRAAQQRGLPVVVATQMLESMISAPAPTRAEASDVA
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHH
TAVFDGADAVMLSAESAAGQYPREAVNMMDRIVARVERDDDWRRTIDAKRPDPEPSSADA
HHHHCCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHH
IARAAEQVAHTIGARAIVAFTSSGASALRVARERPDCPVIGLTPSLDTARRLAAVWGVHA
HHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHH
VVTQDVGSMTEAVALSAKVARQEGFAQPGEEIVVVAGVPFGQSGTTNALRVARV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEECC
>Mature Secondary Structure 
ASRLRRHRRTKIVATIGPASSTPEILSQLFETGADVFRLNFSHGTHEDHLARLQMIRAL
CHHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
ERKFDRPIGILADVQGPKLRVGQFRGGRVQLQTGAEFQLDLNPAPGDTRRVNLPHPEIIE
HHHHCCCCEEEEECCCCCEEEECCCCCEEEEECCCEEEEECCCCCCCCEEECCCCHHHHH
AASIGATLLLDDGKLRLRVLRKREDHLLTEVVTGGPLSDRKGVNVPDVVLPIPALTKKDR
HHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCH
ADLAFALEHGIDYIGLSFVQRAADVLEAKEIAAGRALVMTKMEKPQALDNLDEIMDATDA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCHHCE
VMVARGDLGVELPPEEVPIVQKRIIRAAQQRGLPVVVATQMLESMISAPAPTRAEASDVA
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHH
TAVFDGADAVMLSAESAAGQYPREAVNMMDRIVARVERDDDWRRTIDAKRPDPEPSSADA
HHHHCCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHH
IARAAEQVAHTIGARAIVAFTSSGASALRVARERPDCPVIGLTPSLDTARRLAAVWGVHA
HHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHH
VVTQDVGSMTEAVALSAKVARQEGFAQPGEEIVVVAGVPFGQSGTTNALRVARV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA