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The map label for this gene is thiO/thiG [H]

Identifier: 148243475

GI number: 148243475

Start: 2068353

End: 2069495

Strand: Direct

Name: thiO/thiG [H]

Synonym: SynRCC307_2376

Alternate gene names: 148243475

Gene position: 2068353-2069495 (Clockwise)

Preceding gene: 148243473

Following gene: 148243477

Centisome position: 92.96

GC content: 65.44

Gene sequence:

>1143_bases
TTGAGTGCATTGGCGGCTGCTGAGCCCGTACTGATTCTTGGTGGTGGCTTGATGGGCCTGGCCCTGGCCCATCAGCTGGC
CCGGCGCGGCCAGAGCGTTCAAGTGATCAGCCGACGCCGCAGCGAAGCCGCGGGTTTTGTCGCCGCTGGCATGCTGGCGC
CCCACGCTGAAGGCCTCAGTGGAGAGCTGCTGGCCCTGGGCCAACGCAGCCTGGAGCTGGTGCCCAGCTGGTTGCAGCAA
GTGGAAGCCGATAGCGACCTGGATTGCGGGCATCGGCCCTGCGGCATCGTTGTGCCGTTCCGCAGCGAACAGGAACGCGA
CGCCTACCCCACAGCCAGCTGCGGAGTCGCACTGAACAGAGATGCGCTGGAACGTGAAATCCCCGGCATTGCCGCTGTCT
GGAGCACCGGCTTGCTGTTTGAGCAGGACGGGCAAATTGATAACCGCCGGAGGCTGATGCGCGCCTTGGAGCGGGCTTGC
TCACGGCTGGGGGTGCAGTTCGAAGAAGGCGCTGAAGTGCAGAGCCTTCAAGCCCATAGCCATGGCGCTCTGCAAGCGAT
CAGCCTGCGGCGAGCTGACGGTGAACTCCAGCAGTTGCCCTGCCAGCAGGCCGTGCTCTGCAGTGGCGCCTGGAGCGGCC
AACTGCTGCCGGAGCTGCCAGTGTTCCCGGTCAAAGGCCAAATGCTGTCGCTGCAAGCCCCGCGGCAAGCCCTGCGGCGA
GTGCTATTTGGCCCGGGCACCTATCTGGTGCCAAGAGAAGACGGGCTCTTGGTGGTGGGAGCCACCAGCGAACAAGACGC
CGGATTCAGCGAAGGGCTAACACCTGAAGGACAAAAACAACTCGAGGCCGGTATTGCCGCGTTGCTGCCGGAAGCCAGCC
AGTGGCCACCGATGGAGCGCTGGTGGGGCTTTCGCCCCTGCACACCCGATGAAGGGCCGCTCCTGGGGGCAAGCCCAATG
GCAGGGCTGTGGCTGGCCTGCGGCCACCACCGCAATGGCGTGCTGCTGGCGGCCATCACCGCAAAGCTGCTGGCGGGGGC
CATCACACAAGAAACCCCCAGCAGCGCCGACGCGGCACTGCTGGGGGCCTTTGCTTGGGACCGGTTTAACCGAGGGGATC
AGCCCTCAACGCGCCAACCCTGA

Upstream 100 bases:

>100_bases
CCAATGACGGCTTCCCAATCCGCTGCCATCTGCTGCACTTGCTCAAAGGCTGGGGGGATCCTATGGACCCGCAGGAGCCT
CTACGGTCAGCTGGGGAGCA

Downstream 100 bases:

>100_bases
TCAGAAGGAGTCCAATCATTCAATTCGGAAGGCTGGAACCACAGACCAATTTCGAACTGAGCGGTTTCGGCAGCATCGGA
GCCATGGATCACATTGCGGC

Product: glycine/D-amino acid oxidase family protein

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSALAAAEPVLILGGGLMGLALAHQLARRGQSVQVISRRRSEAAGFVAAGMLAPHAEGLSGELLALGQRSLELVPSWLQQ
VEADSDLDCGHRPCGIVVPFRSEQERDAYPTASCGVALNRDALEREIPGIAAVWSTGLLFEQDGQIDNRRRLMRALERAC
SRLGVQFEEGAEVQSLQAHSHGALQAISLRRADGELQQLPCQQAVLCSGAWSGQLLPELPVFPVKGQMLSLQAPRQALRR
VLFGPGTYLVPREDGLLVVGATSEQDAGFSEGLTPEGQKQLEAGIAALLPEASQWPPMERWWGFRPCTPDEGPLLGASPM
AGLWLACGHHRNGVLLAAITAKLLAGAITQETPSSADAALLGAFAWDRFNRGDQPSTRQP

Sequences:

>Translated_380_residues
MSALAAAEPVLILGGGLMGLALAHQLARRGQSVQVISRRRSEAAGFVAAGMLAPHAEGLSGELLALGQRSLELVPSWLQQ
VEADSDLDCGHRPCGIVVPFRSEQERDAYPTASCGVALNRDALEREIPGIAAVWSTGLLFEQDGQIDNRRRLMRALERAC
SRLGVQFEEGAEVQSLQAHSHGALQAISLRRADGELQQLPCQQAVLCSGAWSGQLLPELPVFPVKGQMLSLQAPRQALRR
VLFGPGTYLVPREDGLLVVGATSEQDAGFSEGLTPEGQKQLEAGIAALLPEASQWPPMERWWGFRPCTPDEGPLLGASPM
AGLWLACGHHRNGVLLAAITAKLLAGAITQETPSSADAALLGAFAWDRFNRGDQPSTRQP
>Mature_379_residues
SALAAAEPVLILGGGLMGLALAHQLARRGQSVQVISRRRSEAAGFVAAGMLAPHAEGLSGELLALGQRSLELVPSWLQQV
EADSDLDCGHRPCGIVVPFRSEQERDAYPTASCGVALNRDALEREIPGIAAVWSTGLLFEQDGQIDNRRRLMRALERACS
RLGVQFEEGAEVQSLQAHSHGALQAISLRRADGELQQLPCQQAVLCSGAWSGQLLPELPVFPVKGQMLSLQAPRQALRRV
LFGPGTYLVPREDGLLVVGATSEQDAGFSEGLTPEGQKQLEAGIAALLPEASQWPPMERWWGFRPCTPDEGPLLGASPMA
GLWLACGHHRNGVLLAAITAKLLAGAITQETPSSADAALLGAFAWDRFNRGDQPSTRQP

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=435, Percent_Identity=24.1379310344828, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 40571; Mature: 40440

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALAAAEPVLILGGGLMGLALAHQLARRGQSVQVISRRRSEAAGFVAAGMLAPHAEGLS
CCCCHHCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GELLALGQRSLELVPSWLQQVEADSDLDCGHRPCGIVVPFRSEQERDAYPTASCGVALNR
CHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEECH
DALEREIPGIAAVWSTGLLFEQDGQIDNRRRLMRALERACSRLGVQFEEGAEVQSLQAHS
HHHHHHCCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
HGALQAISLRRADGELQQLPCQQAVLCSGAWSGQLLPELPVFPVKGQMLSLQAPRQALRR
CCCCEEHHHHHCCCHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHH
VLFGPGTYLVPREDGLLVVGATSEQDAGFSEGLTPEGQKQLEAGIAALLPEASQWPPMER
HHHCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
WWGFRPCTPDEGPLLGASPMAGLWLACGHHRNGVLLAAITAKLLAGAITQETPSSADAAL
HCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHH
LGAFAWDRFNRGDQPSTRQP
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SALAAAEPVLILGGGLMGLALAHQLARRGQSVQVISRRRSEAAGFVAAGMLAPHAEGLS
CCCHHCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GELLALGQRSLELVPSWLQQVEADSDLDCGHRPCGIVVPFRSEQERDAYPTASCGVALNR
CHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEECH
DALEREIPGIAAVWSTGLLFEQDGQIDNRRRLMRALERACSRLGVQFEEGAEVQSLQAHS
HHHHHHCCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
HGALQAISLRRADGELQQLPCQQAVLCSGAWSGQLLPELPVFPVKGQMLSLQAPRQALRR
CCCCEEHHHHHCCCHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHH
VLFGPGTYLVPREDGLLVVGATSEQDAGFSEGLTPEGQKQLEAGIAALLPEASQWPPMER
HHHCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
WWGFRPCTPDEGPLLGASPMAGLWLACGHHRNGVLLAAITAKLLAGAITQETPSSADAAL
HCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHH
LGAFAWDRFNRGDQPSTRQP
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]