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The map label for this gene is prfA

Identifier: 148243244

GI number: 148243244

Start: 1847597

End: 1848691

Strand: Direct

Name: prfA

Synonym: SynRCC307_2145

Alternate gene names: 148243244

Gene position: 1847597-1848691 (Clockwise)

Preceding gene: 148243243

Following gene: 148243246

Centisome position: 83.04

GC content: 61.0

Gene sequence:

>1095_bases
GTGGATCAGTCCTTTGTCACCGAGCGGCTAGAAGCCACCTGCCGCACCTTCAACGCCCTCGAGCGTCAGCTGGCTGACCC
CAGCGTGGCGGCTGACCCTGAACAACTGCTGACCCTGGCCAAAGAGCGCTCGAGGCTTGAGCCCCTGGTTCTCGATTACC
AACGGCTGCAACAACTCCACGCCGAGCATCAGCAGGCCCAGCAATTGCTCAAAGAGAGCAAGGGCGACGCTGAACTGGAG
GCGTTGGCCCAAGAGGAGCTGCAGCAACTGAGCTCAGAGCAGGAGCAGCTCAACCAACGCCTGAAAGTGGCTCTGCTGCC
CAGCGACCCCCGCGATGAGCGCAGCGTGATGCTGGAAATTCGCGCCGGAGCCGGCGGTGATGAAGCCTGCCTGTGGGCTG
GCGATCTCGCCCGCATGTATGAACGCCACGCCCAAACCTGCGGCTGGCAAGTGAATCCCGTCAGCGCTTCAGAAGCCGAG
CTCGGTGGCTTCAAGGAATTAATCCTGGCGATCCGCGGGGATGCGGTGTTCAGCCAACTCAAATACGAAGCCGGTGTGCA
TCGGGTGCAGCGGGTACCCGCCACGGAATCACAGGGTCGTGTTCACACCTCAACAGCCACGGTGGCGGTGATGCCAGAGG
CCGATCCGGTTGATGTGCAAATCGATCCCAAGGATCTCGACATCAGCACGGCCCGCTCTGGCGGTGCTGGAGGGCAAAAC
GTCAACAAAGTGGAAACAGCCGTCGATCTGCTGCACAAGCCCACCGGGATTCGGGTGTTCTGCACCCAGGAACGCTCCCA
GCTGCAAAACCGCGAGCGTGCCATGGAGATTCTGCGGGCCAAGCTGCTGGCCAAAGAGGAGGAAGAAGCGGCAGCAGCCG
AGAGTTCGGCGCGCCGCGCCCAAGTGGGCAGCGGTGATCGCAGCGAAAAAATTCGCACCTACAACTACAAAGACAACCGC
ACCACCGATCACCGCCTGGGCAAAAACTTCCCATTGGAAACCGTGCTCAACGGCCAACTCAGCGATCTAATCGAGGCCTG
CACCCATGCTGATCAGCAACAAAAACTGGAAGAGCTCGCGGCCAGCGAGAGCTAG

Upstream 100 bases:

>100_bases
CGACAGCTGAACTACGCAGCTGCAATGACGTCTGAGATGGCCGGGTGCTCCCAACGGGGCATCCGGCTTTTCTATCGGCG
CTGCTCAACCCGCTGAATCT

Downstream 100 bases:

>100_bases
GCGCCAATCACGTATTTGCGCCACTCTTCCCGGCCGCTGCGAATCTGGCGGGTGGCTTCGAAATACAAGGTGCTCACCGG
GCGTCTTGGCACCGAGTTCA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MDQSFVTERLEATCRTFNALERQLADPSVAADPEQLLTLAKERSRLEPLVLDYQRLQQLHAEHQQAQQLLKESKGDAELE
ALAQEELQQLSSEQEQLNQRLKVALLPSDPRDERSVMLEIRAGAGGDEACLWAGDLARMYERHAQTCGWQVNPVSASEAE
LGGFKELILAIRGDAVFSQLKYEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVDVQIDPKDLDISTARSGGAGGQN
VNKVETAVDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLLAKEEEEAAAAESSARRAQVGSGDRSEKIRTYNYKDNR
TTDHRLGKNFPLETVLNGQLSDLIEACTHADQQQKLEELAASES

Sequences:

>Translated_364_residues
MDQSFVTERLEATCRTFNALERQLADPSVAADPEQLLTLAKERSRLEPLVLDYQRLQQLHAEHQQAQQLLKESKGDAELE
ALAQEELQQLSSEQEQLNQRLKVALLPSDPRDERSVMLEIRAGAGGDEACLWAGDLARMYERHAQTCGWQVNPVSASEAE
LGGFKELILAIRGDAVFSQLKYEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVDVQIDPKDLDISTARSGGAGGQN
VNKVETAVDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLLAKEEEEAAAAESSARRAQVGSGDRSEKIRTYNYKDNR
TTDHRLGKNFPLETVLNGQLSDLIEACTHADQQQKLEELAASES
>Mature_364_residues
MDQSFVTERLEATCRTFNALERQLADPSVAADPEQLLTLAKERSRLEPLVLDYQRLQQLHAEHQQAQQLLKESKGDAELE
ALAQEELQQLSSEQEQLNQRLKVALLPSDPRDERSVMLEIRAGAGGDEACLWAGDLARMYERHAQTCGWQVNPVSASEAE
LGGFKELILAIRGDAVFSQLKYEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVDVQIDPKDLDISTARSGGAGGQN
VNKVETAVDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLLAKEEEEAAAAESSARRAQVGSGDRSEKIRTYNYKDNR
TTDHRLGKNFPLETVLNGQLSDLIEACTHADQQQKLEELAASES

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=303, Percent_Identity=42.5742574257426, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI34577120, Length=325, Percent_Identity=36.9230769230769, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI166795305, Length=194, Percent_Identity=41.2371134020619, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=45.5555555555556, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=37.125748502994, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=322, Percent_Identity=36.6459627329193, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=298, Percent_Identity=40.6040268456376, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI19921226, Length=296, Percent_Identity=41.554054054054, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SYNR3 (A5GVY9)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001228401.1
- ProteinModelPortal:   A5GVY9
- SMR:   A5GVY9
- STRING:   A5GVY9
- GeneID:   5156908
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_2145
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40358; Mature: 40358

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQSFVTERLEATCRTFNALERQLADPSVAADPEQLLTLAKERSRLEPLVLDYQRLQQLH
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AEHQQAQQLLKESKGDAELEALAQEELQQLSSEQEQLNQRLKVALLPSDPRDERSVMLEI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEE
RAGAGGDEACLWAGDLARMYERHAQTCGWQVNPVSASEAELGGFKELILAIRGDAVFSQL
ECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
KYEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVDVQIDPKDLDISTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCC
VNKVETAVDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLLAKEEEEAAAAESSARRA
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGSGDRSEKIRTYNYKDNRTTDHRLGKNFPLETVLNGQLSDLIEACTHADQQQKLEELA
HCCCCCCCCCCEEECCCCCCCHHHHCCCCCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHH
ASES
CCCC
>Mature Secondary Structure
MDQSFVTERLEATCRTFNALERQLADPSVAADPEQLLTLAKERSRLEPLVLDYQRLQQLH
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AEHQQAQQLLKESKGDAELEALAQEELQQLSSEQEQLNQRLKVALLPSDPRDERSVMLEI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEE
RAGAGGDEACLWAGDLARMYERHAQTCGWQVNPVSASEAELGGFKELILAIRGDAVFSQL
ECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
KYEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVDVQIDPKDLDISTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCC
VNKVETAVDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLLAKEEEEAAAAESSARRA
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGSGDRSEKIRTYNYKDNRTTDHRLGKNFPLETVLNGQLSDLIEACTHADQQQKLEELA
HCCCCCCCCCCEEECCCCCCCHHHHCCCCCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHH
ASES
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA