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The map label for this gene is nadE [H]

Identifier: 148242969

GI number: 148242969

Start: 1629010

End: 1630686

Strand: Direct

Name: nadE [H]

Synonym: SynRCC307_1870

Alternate gene names: 148242969

Gene position: 1629010-1630686 (Clockwise)

Preceding gene: 148242968

Following gene: 148242970

Centisome position: 73.22

GC content: 64.04

Gene sequence:

>1677_bases
GTGCGGATCGCACTGATCCAATCCAACCCGCTGGTCGGTGATCTGCAGGGCAATCGGCAACGCCTGCAACAGCAGTGCTT
GCAGGCTGCCGAAGCCGGAGCCGAACTCGCCCTGGCCCCTGAACTCGCCCTCTGGGGCTATCCGCCCCGGGATCTGCTGT
TGCAGCCGGCCCTACGGCAGCAACAAGACCGCCAACTCGATCAGCTCAGTGGCGCGTTGCCGCCGGGGTTTGGGCTGCTG
CTTGGGGTGGTTGAAACCAGTGGCCAGAAGCTTTTCAACGCCATGGCCCTGGTGGAGCGCGGCGGCTGGCGCTTGGTGGC
GCGCAAACGGTTGCTGCCCAGTTACGACGTCTTCGATGAACAGCGCTATTTCCACAGCGGTGATCAACCCGCGCTGCTGA
GCTGGGGCGGTCAGCGCCTTGGGCTGACCCTGTGCGAAGACCTCTGGGTTGAACCCCAAATCAGCGGCCGCAATGCGCCG
CTGTGCGACCCAATTGCTGAACTTCAGCCCCTCAAGCCGGATCTATTGATCAACCTCTCAGCGTCCCCCTTTGGCCAGGG
CAAAGCGGAGCGTCGCCGGCAGCTGGCGGGGGCTGCCCAGCAGCGGTTGGGATGCCCAGTGCTCTACGTCAATCAGGTGG
GAGGAAACGACGAACTGATTTTTGATGGCAACAGCTTTGTGCTGGATGCCACTGGGGCGCTGCAGCAGCAACTGCCCTGC
GGCGAGAGCGCGCTGCAGATTTGGGACAGCCAGAGCGCCACAAGCCCAACCCCACCCGAGCCCCTGAGCCCCGAGGCTCA
GCTCTGGCGCATGCTCGTGCTCGGGGTGCAGGACTACGCCCGCAAATGCGGCTTCCGCAAAGCCTTGCTCGGGCTCAGTG
GCGGCATTGATTCGGCCCTGGTGGCAGCCCTAGCCGTTGCGGCTCTGGGCAGTCAGCAGGTGCGGGCGCTGTTGATGCCA
TCGCCCTACAGCTCTGAAGGCTCGCTGCAGGATTCCCTGGCCCTCGCCAGTCGGTTGAATCTGGCCACCAGCACGGTGCC
GATTGAGCCCTTGATGCAGCAATACGACAGCAGCTTGGATCCAGCGCTGGGGCAGAAGCCCGAGGGCATCACCGCCGAAA
ACCTGCAATCGCGCATCCGCGGCACGTTGCTGATGGCCGTGGCCAATAGCGAAGGCCAACTGCTGCTAGCGACCGGCAAC
AAATCCGAGCTGGCCGTTGGTTACTGCACGCTCTACGGCGACATGAATGGCGGCCTGGCCGTCATTGGGGATCTCTACAA
AAGCGAGGTGTTTCGGCTGACGCGCTGGCTCGATAGCCCGGCTGCTGCCGGCGAGCGGCAACGCTGCGGGCTGCCAGCCG
ATGGGCCGGTGATCGGGGACGCGATCTTGAATAAGCCGCCCAGCGCCGAGCTGAGACCGGATCAAAAAGACAGCGACTCC
CTGCCCGACTACCCCTATCTCGATCGCCTACTGCAGCGCTTTATCGAGGAGCATGCCTCGCCGGAAGAGCTCATCGCCAG
CGGGGAGCCGAGCGACGCCGTGCAGCGGATCTGGCGGCTGCTGAAACGGTCAGAATTCAAACGGCGCCAAGCTGCACCGG
TGCTCAAACTGAGTGCTCGCTCCTTCGGTTGCGGTTGGCGCATGCCAATCTCCGCCAGAACTGGCGCCGAAGGCTAG

Upstream 100 bases:

>100_bases
TCAGTGCGGCATCACCCCAACCCCGAACTGGTCCCCAGCGAGTTGCTCACGGACTTAGCGCAGCGAAACCTTTACGGTTT
CAGCATCGACGACCTCCCCT

Downstream 100 bases:

>100_bases
GAATCGCCAGGCTGATAGCAGAACCAACCCACCATGGCCGCTCCCCAACCCGCCGCCTCGATGAGCAGCATCGGCATTCC
TCGGGAGATCAAGCCCGATG

Product: glutamine-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MRIALIQSNPLVGDLQGNRQRLQQQCLQAAEAGAELALAPELALWGYPPRDLLLQPALRQQQDRQLDQLSGALPPGFGLL
LGVVETSGQKLFNAMALVERGGWRLVARKRLLPSYDVFDEQRYFHSGDQPALLSWGGQRLGLTLCEDLWVEPQISGRNAP
LCDPIAELQPLKPDLLINLSASPFGQGKAERRRQLAGAAQQRLGCPVLYVNQVGGNDELIFDGNSFVLDATGALQQQLPC
GESALQIWDSQSATSPTPPEPLSPEAQLWRMLVLGVQDYARKCGFRKALLGLSGGIDSALVAALAVAALGSQQVRALLMP
SPYSSEGSLQDSLALASRLNLATSTVPIEPLMQQYDSSLDPALGQKPEGITAENLQSRIRGTLLMAVANSEGQLLLATGN
KSELAVGYCTLYGDMNGGLAVIGDLYKSEVFRLTRWLDSPAAAGERQRCGLPADGPVIGDAILNKPPSAELRPDQKDSDS
LPDYPYLDRLLQRFIEEHASPEELIASGEPSDAVQRIWRLLKRSEFKRRQAAPVLKLSARSFGCGWRMPISARTGAEG

Sequences:

>Translated_558_residues
MRIALIQSNPLVGDLQGNRQRLQQQCLQAAEAGAELALAPELALWGYPPRDLLLQPALRQQQDRQLDQLSGALPPGFGLL
LGVVETSGQKLFNAMALVERGGWRLVARKRLLPSYDVFDEQRYFHSGDQPALLSWGGQRLGLTLCEDLWVEPQISGRNAP
LCDPIAELQPLKPDLLINLSASPFGQGKAERRRQLAGAAQQRLGCPVLYVNQVGGNDELIFDGNSFVLDATGALQQQLPC
GESALQIWDSQSATSPTPPEPLSPEAQLWRMLVLGVQDYARKCGFRKALLGLSGGIDSALVAALAVAALGSQQVRALLMP
SPYSSEGSLQDSLALASRLNLATSTVPIEPLMQQYDSSLDPALGQKPEGITAENLQSRIRGTLLMAVANSEGQLLLATGN
KSELAVGYCTLYGDMNGGLAVIGDLYKSEVFRLTRWLDSPAAAGERQRCGLPADGPVIGDAILNKPPSAELRPDQKDSDS
LPDYPYLDRLLQRFIEEHASPEELIASGEPSDAVQRIWRLLKRSEFKRRQAAPVLKLSARSFGCGWRMPISARTGAEG
>Mature_558_residues
MRIALIQSNPLVGDLQGNRQRLQQQCLQAAEAGAELALAPELALWGYPPRDLLLQPALRQQQDRQLDQLSGALPPGFGLL
LGVVETSGQKLFNAMALVERGGWRLVARKRLLPSYDVFDEQRYFHSGDQPALLSWGGQRLGLTLCEDLWVEPQISGRNAP
LCDPIAELQPLKPDLLINLSASPFGQGKAERRRQLAGAAQQRLGCPVLYVNQVGGNDELIFDGNSFVLDATGALQQQLPC
GESALQIWDSQSATSPTPPEPLSPEAQLWRMLVLGVQDYARKCGFRKALLGLSGGIDSALVAALAVAALGSQQVRALLMP
SPYSSEGSLQDSLALASRLNLATSTVPIEPLMQQYDSSLDPALGQKPEGITAENLQSRIRGTLLMAVANSEGQLLLATGN
KSELAVGYCTLYGDMNGGLAVIGDLYKSEVFRLTRWLDSPAAAGERQRCGLPADGPVIGDAILNKPPSAELRPDQKDSDS
LPDYPYLDRLLQRFIEEHASPEELIASGEPSDAVQRIWRLLKRSEFKRRQAAPVLKLSARSFGCGWRMPISARTGAEG

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60452; Mature: 60452

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALIQSNPLVGDLQGNRQRLQQQCLQAAEAGAELALAPELALWGYPPRDLLLQPALRQ
CEEEEEECCCCEECCCCCHHHHHHHHHHHHHCCCCEEECCCHHHCCCCCHHHHHCHHHHH
QQDRQLDQLSGALPPGFGLLLGVVETSGQKLFNAMALVERGGWRLVARKRLLPSYDVFDE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCHH
QRYFHSGDQPALLSWGGQRLGLTLCEDLWVEPQISGRNAPLCDPIAELQPLKPDLLINLS
HHHHCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEC
ASPFGQGKAERRRQLAGAAQQRLGCPVLYVNQVGGNDELIFDGNSFVLDATGALQQQLPC
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCEEEEECCHHHHHHCCC
GESALQIWDSQSATSPTPPEPLSPEAQLWRMLVLGVQDYARKCGFRKALLGLSGGIDSAL
CHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHH
VAALAVAALGSQQVRALLMPSPYSSEGSLQDSLALASRLNLATSTVPIEPLMQQYDSSLD
HHHHHHHHHCCHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
PALGQKPEGITAENLQSRIRGTLLMAVANSEGQLLLATGNKSELAVGYCTLYGDMNGGLA
CCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCEEEEECCCCCEEEEEEEEEECCCCCHH
VIGDLYKSEVFRLTRWLDSPAAAGERQRCGLPADGPVIGDAILNKPPSAELRPDQKDSDS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECHHHHCCCCCCCCCCCCCCCCC
LPDYPYLDRLLQRFIEEHASPEELIASGEPSDAVQRIWRLLKRSEFKRRQAAPVLKLSAR
CCCCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
SFGCGWRMPISARTGAEG
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIALIQSNPLVGDLQGNRQRLQQQCLQAAEAGAELALAPELALWGYPPRDLLLQPALRQ
CEEEEEECCCCEECCCCCHHHHHHHHHHHHHCCCCEEECCCHHHCCCCCHHHHHCHHHHH
QQDRQLDQLSGALPPGFGLLLGVVETSGQKLFNAMALVERGGWRLVARKRLLPSYDVFDE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCHH
QRYFHSGDQPALLSWGGQRLGLTLCEDLWVEPQISGRNAPLCDPIAELQPLKPDLLINLS
HHHHCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEC
ASPFGQGKAERRRQLAGAAQQRLGCPVLYVNQVGGNDELIFDGNSFVLDATGALQQQLPC
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCEEEEECCHHHHHHCCC
GESALQIWDSQSATSPTPPEPLSPEAQLWRMLVLGVQDYARKCGFRKALLGLSGGIDSAL
CHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHH
VAALAVAALGSQQVRALLMPSPYSSEGSLQDSLALASRLNLATSTVPIEPLMQQYDSSLD
HHHHHHHHHCCHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
PALGQKPEGITAENLQSRIRGTLLMAVANSEGQLLLATGNKSELAVGYCTLYGDMNGGLA
CCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCEEEEECCCCCEEEEEEEEEECCCCCHH
VIGDLYKSEVFRLTRWLDSPAAAGERQRCGLPADGPVIGDAILNKPPSAELRPDQKDSDS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECHHHHCCCCCCCCCCCCCCCCC
LPDYPYLDRLLQRFIEEHASPEELIASGEPSDAVQRIWRLLKRSEFKRRQAAPVLKLSAR
CCCCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
SFGCGWRMPISARTGAEG
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]