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The map label for this gene is dnaK [H]

Identifier: 148242952

GI number: 148242952

Start: 1612683

End: 1614257

Strand: Direct

Name: dnaK [H]

Synonym: SynRCC307_1853

Alternate gene names: 148242952

Gene position: 1612683-1614257 (Clockwise)

Preceding gene: 148242948

Following gene: 148242953

Centisome position: 72.48

GC content: 65.33

Gene sequence:

>1575_bases
GTGTGGATGGCCGTACTTTCTGCAATGGTTTCAGCATTCAGGAAAGGATTTGCCTTGAGCCAGGGGAGCCTTGCGATCGA
CTTGGGCAGCTCAAGCACCCTGGTGGGCTGGCAAGCCGCACCTGGCAGCCCTGGGGAAGTGCTGGCTTTAGCGCCCCTCA
GCCGCTCCACAAGCGATGCCAGCATTCCCAGCCTGGTCAGCCCCAGCGGCGGCAGCCGTTGGCTGTTGGGGCAGCAAGTG
ATCGAAGCCGGCAGCGAAGCCGAGTGCCTGCGGGATTTCAAAGCGCTGCTCGGGCAAAGCAATGCTCCAACCGCAGCTGA
GCAGGCTGCTGAGGCCTTGCTTCAAGGCATTTGGCAACGGCTGCCGCAGGCCATCCAGCCCCAACGGCTGGTGCTCACCG
CGCCGGTCCAGGGCTTCGCGGGGTATCGGCGCTGGCTGCAGCGGTGGGCCGAGTCCCTCGCCATTGATGAAGTGGCCCTG
GTGGATGAACCCACAGCAGCTGCCCTTGGCGCAGGCCTATCGCCTGGAAGCCTGGTGCTGGTGCTCGATATCGGCGCCGG
CACCACCGATCTGGCGCTGGTCAGGCTGGAGGGGGGCGAAGGCCGTGCCATGCCCATGGCCCAACTGCTGCGCTTTGCGG
GCCGCTCTCTAGCGGAACGTCCAGGACAACAACAGCGCACCGCCAAGGTGCTGGGCAAAGCGGGAATCAGCCTGGGGGGG
CGCATGATCGATCGCTGGTGGGCCAAAGCTTTAGGAGCCCCTGAGCCAGCTCCGCAGGCCTGGTTGAACGCTGCTGAGGC
ACTCAAATGCGCCTTAAGCGAAACCACCAGCGCTCAAGTGGTGCTGGAAAGCGAGGCAGGGCCCCGGCCGTTGCAAGGAA
GCCGGCGGGAGCTCAACAACGTGCTGGACGCCGCCGGCTTGGAGCCGCTCTTGGATGGCCTGCTCAATAAAGTGGAGGCT
GCGGCCCGGCGGGCCGGCGAATCCCTCGATTCCATCGATGCGGTGATGGCCGTCGGTGGCGGCAGCGCACTGCCCTGGGT
GCAGCAGTGGCTGCAGCGCCGCTTGCCGAAAACAGAGCTCCTGGTCACCCAACCCCTGCAAGCGGTGGTGCTCGGCGCCT
TGGCCATGACTCCAGCTCTGCAGGTGATGGATGTGTTGCAACACGGCATCAGCCTGCGCTGCTGGGACCGGAGGCTGCAG
AACCAGCGCTGGCATCCCCTGTTTTTACCCGGGCAAGCCTGGCCAACCCCGCAACCCCTGGAGCTGGTTTTAGCCAGCCG
CGGTGATCAAGCCTGCGTTGAGGTGCAACTGGGCACCCCGAGCGGTGAATCCAGGGCCGAGGTGGTGTTTGTCGATGGTC
TGCCGGTGCTCCGCAGTCAGGAGGCCGGCGAGGCCAGCGTGCGGCCCTGGAACCAACCGGCGTTGGAGATCCCTCTGCCA
GCGGGCGCTCAAGAAGGCCAAGACTGCCTGCGGCTGCGCTTCGGGGTCGACGCCGAACGGCAGCTTTGGCTGGAGGGGTT
TGATCTTGTTGGCGAGCAACGACTCTCCCGCCAAATCCTCGGCATTGTGGAGTGA

Upstream 100 bases:

>100_bases
TCTTCGGTCTCCTCCCGATAAAACGAGAGCACAGCCCGCAACAACCGCTCGCCAGCCATCTCATACCCGGACTCATTGAT
CTCAATGTGAACCGCGCTGG

Downstream 100 bases:

>100_bases
CTTGCCCTCCCCCGGTGTCGCCGCGCCGCTCGCGACTACCCAAGGTTTGGATCTGGAGCTCCATCAGCCTGGTGGGGGCC
GTGGCGCTGTCGTTGGGCTG

Product: molecular chaperone DnaK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MWMAVLSAMVSAFRKGFALSQGSLAIDLGSSSTLVGWQAAPGSPGEVLALAPLSRSTSDASIPSLVSPSGGSRWLLGQQV
IEAGSEAECLRDFKALLGQSNAPTAAEQAAEALLQGIWQRLPQAIQPQRLVLTAPVQGFAGYRRWLQRWAESLAIDEVAL
VDEPTAAALGAGLSPGSLVLVLDIGAGTTDLALVRLEGGEGRAMPMAQLLRFAGRSLAERPGQQQRTAKVLGKAGISLGG
RMIDRWWAKALGAPEPAPQAWLNAAEALKCALSETTSAQVVLESEAGPRPLQGSRRELNNVLDAAGLEPLLDGLLNKVEA
AARRAGESLDSIDAVMAVGGGSALPWVQQWLQRRLPKTELLVTQPLQAVVLGALAMTPALQVMDVLQHGISLRCWDRRLQ
NQRWHPLFLPGQAWPTPQPLELVLASRGDQACVEVQLGTPSGESRAEVVFVDGLPVLRSQEAGEASVRPWNQPALEIPLP
AGAQEGQDCLRLRFGVDAERQLWLEGFDLVGEQRLSRQILGIVE

Sequences:

>Translated_524_residues
MWMAVLSAMVSAFRKGFALSQGSLAIDLGSSSTLVGWQAAPGSPGEVLALAPLSRSTSDASIPSLVSPSGGSRWLLGQQV
IEAGSEAECLRDFKALLGQSNAPTAAEQAAEALLQGIWQRLPQAIQPQRLVLTAPVQGFAGYRRWLQRWAESLAIDEVAL
VDEPTAAALGAGLSPGSLVLVLDIGAGTTDLALVRLEGGEGRAMPMAQLLRFAGRSLAERPGQQQRTAKVLGKAGISLGG
RMIDRWWAKALGAPEPAPQAWLNAAEALKCALSETTSAQVVLESEAGPRPLQGSRRELNNVLDAAGLEPLLDGLLNKVEA
AARRAGESLDSIDAVMAVGGGSALPWVQQWLQRRLPKTELLVTQPLQAVVLGALAMTPALQVMDVLQHGISLRCWDRRLQ
NQRWHPLFLPGQAWPTPQPLELVLASRGDQACVEVQLGTPSGESRAEVVFVDGLPVLRSQEAGEASVRPWNQPALEIPLP
AGAQEGQDCLRLRFGVDAERQLWLEGFDLVGEQRLSRQILGIVE
>Mature_524_residues
MWMAVLSAMVSAFRKGFALSQGSLAIDLGSSSTLVGWQAAPGSPGEVLALAPLSRSTSDASIPSLVSPSGGSRWLLGQQV
IEAGSEAECLRDFKALLGQSNAPTAAEQAAEALLQGIWQRLPQAIQPQRLVLTAPVQGFAGYRRWLQRWAESLAIDEVAL
VDEPTAAALGAGLSPGSLVLVLDIGAGTTDLALVRLEGGEGRAMPMAQLLRFAGRSLAERPGQQQRTAKVLGKAGISLGG
RMIDRWWAKALGAPEPAPQAWLNAAEALKCALSETTSAQVVLESEAGPRPLQGSRRELNNVLDAAGLEPLLDGLLNKVEA
AARRAGESLDSIDAVMAVGGGSALPWVQQWLQRRLPKTELLVTQPLQAVVLGALAMTPALQVMDVLQHGISLRCWDRRLQ
NQRWHPLFLPGQAWPTPQPLELVLASRGDQACVEVQLGTPSGESRAEVVFVDGLPVLRSQEAGEASVRPWNQPALEIPLP
AGAQEGQDCLRLRFGVDAERQLWLEGFDLVGEQRLSRQILGIVE

Specific function: Chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by hscB [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

None

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126
- InterPro:   IPR010236 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 55837; Mature: 55837

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWMAVLSAMVSAFRKGFALSQGSLAIDLGSSSTLVGWQAAPGSPGEVLALAPLSRSTSDA
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCC
SIPSLVSPSGGSRWLLGQQVIEAGSEAECLRDFKALLGQSNAPTAAEQAAEALLQGIWQR
CCCCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LPQAIQPQRLVLTAPVQGFAGYRRWLQRWAESLAIDEVALVDEPTAAALGAGLSPGSLVL
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEE
VLDIGAGTTDLALVRLEGGEGRAMPMAQLLRFAGRSLAERPGQQQRTAKVLGKAGISLGG
EEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHCHHHHH
RMIDRWWAKALGAPEPAPQAWLNAAEALKCALSETTSAQVVLESEAGPRPLQGSRRELNN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHH
VLDAAGLEPLLDGLLNKVEAAARRAGESLDSIDAVMAVGGGSALPWVQQWLQRRLPKTEL
HHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHH
LVTQPLQAVVLGALAMTPALQVMDVLQHGISLRCWDRRLQNQRWHPLFLPGQAWPTPQPL
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCEEEECCCCCCCCCHH
ELVLASRGDQACVEVQLGTPSGESRAEVVFVDGLPVLRSQEAGEASVRPWNQPALEIPLP
HHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHCCCCCCCCCCCCCCCCCEEECCC
AGAQEGQDCLRLRFGVDAERQLWLEGFDLVGEQRLSRQILGIVE
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWMAVLSAMVSAFRKGFALSQGSLAIDLGSSSTLVGWQAAPGSPGEVLALAPLSRSTSDA
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCC
SIPSLVSPSGGSRWLLGQQVIEAGSEAECLRDFKALLGQSNAPTAAEQAAEALLQGIWQR
CCCCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LPQAIQPQRLVLTAPVQGFAGYRRWLQRWAESLAIDEVALVDEPTAAALGAGLSPGSLVL
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEE
VLDIGAGTTDLALVRLEGGEGRAMPMAQLLRFAGRSLAERPGQQQRTAKVLGKAGISLGG
EEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHCHHHHH
RMIDRWWAKALGAPEPAPQAWLNAAEALKCALSETTSAQVVLESEAGPRPLQGSRRELNN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHH
VLDAAGLEPLLDGLLNKVEAAARRAGESLDSIDAVMAVGGGSALPWVQQWLQRRLPKTEL
HHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHH
LVTQPLQAVVLGALAMTPALQVMDVLQHGISLRCWDRRLQNQRWHPLFLPGQAWPTPQPL
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCEEEECCCCCCCCCHH
ELVLASRGDQACVEVQLGTPSGESRAEVVFVDGLPVLRSQEAGEASVRPWNQPALEIPLP
HHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHCCCCCCCCCCCCCCCCCEEECCC
AGAQEGQDCLRLRFGVDAERQLWLEGFDLVGEQRLSRQILGIVE
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA