Click here to switch to the map view.

The map label for this gene is rpoD [H]

Identifier: 148242910

GI number: 148242910

Start: 1574460

End: 1575341

Strand: Direct

Name: rpoD [H]

Synonym: SynRCC307_1811

Alternate gene names: 148242910

Gene position: 1574460-1575341 (Clockwise)

Preceding gene: 148242909

Following gene: 148242911

Centisome position: 70.76

GC content: 63.95

Gene sequence:

>882_bases
ATGGTGAAAGGCAGCAACGATTGCTTTGGCTGGATGCTCAATGGCATCGGCCGTCATGCCCTCTTAAGCCCCGAAGAAGA
GGTGAGCCTGGGTCGCCAAGTGCGCGCCTGGCAGGACCACCCCGGCGGCCCTGGGCAAGCACCAGCTGCCGTGCAAAAGC
GTGGGCTTAGGGCCCGCGGCCGGCTGGTGCAAGCCAACCTGCGGCTGGTGGTGCACCTGGCCAAAAAACAGCACCTGCGC
GGCTTGGCCATGGAAGATTTGGTGCAGGAGGGCTCGATTGGTTTGCAGCGCGCCGCTGAGAAATACGACCCCAGCACCGG
CTACCGCTTCAGCACCTACGCCTATTGGTGGATCAAGCAAGCCCTGCACCGGGCAATTCAGCTGCAAACCCCAGCCCTGC
GGCTGCCCTGCCGGGTGAGCGAGCGGTTGGGGCGACTACGCAGCACCAGGCAACGGCTCAGCCAAGAGCTGGGACGCCAA
CCCAGGCCAGAGGAGCTCGCTGAGGCCATGGGCGAACGCATCGATCAACTCCAAGAGCTCGAGCGCGCCCAGGTGATCAG
CCGCGGCTGTTCCCTCGATACCCCGATGGGCTCAGACCCCGACCACGGCAGCTTGGTGGACTTACTGGAAGATGACCGCA
CCAGTCCCATGGACCAGCTGGAGCAAGCCCTGCGCAGCGAGCAACTGGCAGAACTGCTCAGCAGCGCTGGGCTAAGCGAG
CGCGAAGCCACCGTGGTGCAAGCGCGGCATAGCGAAGAGCACATCCCCTACCAACAACTTGCCGATCGTTTTGGCATCAG
CCGTGAACGCACACGGCAGCTAGAGCACAACGCCCTACGCAAGCTGCGCCACTGCGCTGAAGCTCAAACGGCAGCGGCGT
AG

Upstream 100 bases:

>100_bases
TCGACGCCTGAGGAGAGCCGAACCAAAGCGTCCGGTTCTCCCCATCGCCCCAAACGGCTGAGCGTTTCACCGTGAAGGGA
GAGCCGCAAGGAGCGCGGTG

Downstream 100 bases:

>100_bases
GTTGAAATCCCTCAGCGCGATGGCATGGCTGCGCGGCGCCGAATCAGTCCCAAATGGCAGTGGTACCTGCGGGCTCAAGT
GGGAGTCCTACTGCTGCCAG

Product: RNA polymerase sigma factor, sigma-70 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MVKGSNDCFGWMLNGIGRHALLSPEEEVSLGRQVRAWQDHPGGPGQAPAAVQKRGLRARGRLVQANLRLVVHLAKKQHLR
GLAMEDLVQEGSIGLQRAAEKYDPSTGYRFSTYAYWWIKQALHRAIQLQTPALRLPCRVSERLGRLRSTRQRLSQELGRQ
PRPEELAEAMGERIDQLQELERAQVISRGCSLDTPMGSDPDHGSLVDLLEDDRTSPMDQLEQALRSEQLAELLSSAGLSE
REATVVQARHSEEHIPYQQLADRFGISRERTRQLEHNALRKLRHCAEAQTAAA

Sequences:

>Translated_293_residues
MVKGSNDCFGWMLNGIGRHALLSPEEEVSLGRQVRAWQDHPGGPGQAPAAVQKRGLRARGRLVQANLRLVVHLAKKQHLR
GLAMEDLVQEGSIGLQRAAEKYDPSTGYRFSTYAYWWIKQALHRAIQLQTPALRLPCRVSERLGRLRSTRQRLSQELGRQ
PRPEELAEAMGERIDQLQELERAQVISRGCSLDTPMGSDPDHGSLVDLLEDDRTSPMDQLEQALRSEQLAELLSSAGLSE
REATVVQARHSEEHIPYQQLADRFGISRERTRQLEHNALRKLRHCAEAQTAAA
>Mature_293_residues
MVKGSNDCFGWMLNGIGRHALLSPEEEVSLGRQVRAWQDHPGGPGQAPAAVQKRGLRARGRLVQANLRLVVHLAKKQHLR
GLAMEDLVQEGSIGLQRAAEKYDPSTGYRFSTYAYWWIKQALHRAIQLQTPALRLPCRVSERLGRLRSTRQRLSQELGRQ
PRPEELAEAMGERIDQLQELERAQVISRGCSLDTPMGSDPDHGSLVDLLEDDRTSPMDQLEQALRSEQLAELLSSAGLSE
REATVVQARHSEEHIPYQQLADRFGISRERTRQLEHNALRKLRHCAEAQTAAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=233, Percent_Identity=40.7725321888412, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1789098, Length=292, Percent_Identity=34.5890410958904, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1789871, Length=241, Percent_Identity=33.6099585062241, Blast_Score=111, Evalue=5e-26,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32979; Mature: 32979

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKGSNDCFGWMLNGIGRHALLSPEEEVSLGRQVRAWQDHPGGPGQAPAAVQKRGLRARG
CCCCCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHH
RLVQANLRLVVHLAKKQHLRGLAMEDLVQEGSIGLQRAAEKYDPSTGYRFSTYAYWWIKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ALHRAIQLQTPALRLPCRVSERLGRLRSTRQRLSQELGRQPRPEELAEAMGERIDQLQEL
HHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ERAQVISRGCSLDTPMGSDPDHGSLVDLLEDDRTSPMDQLEQALRSEQLAELLSSAGLSE
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCH
REATVVQARHSEEHIPYQQLADRFGISRERTRQLEHNALRKLRHCAEAQTAAA
HHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKGSNDCFGWMLNGIGRHALLSPEEEVSLGRQVRAWQDHPGGPGQAPAAVQKRGLRARG
CCCCCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHH
RLVQANLRLVVHLAKKQHLRGLAMEDLVQEGSIGLQRAAEKYDPSTGYRFSTYAYWWIKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ALHRAIQLQTPALRLPCRVSERLGRLRSTRQRLSQELGRQPRPEELAEAMGERIDQLQEL
HHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ERAQVISRGCSLDTPMGSDPDHGSLVDLLEDDRTSPMDQLEQALRSEQLAELLSSAGLSE
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCH
REATVVQARHSEEHIPYQQLADRFGISRERTRQLEHNALRKLRHCAEAQTAAA
HHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA