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The map label for this gene is rpoD [H]

Identifier: 148242803

GI number: 148242803

Start: 1488002

End: 1488931

Strand: Direct

Name: rpoD [H]

Synonym: SynRCC307_1704

Alternate gene names: 148242803

Gene position: 1488002-1488931 (Clockwise)

Preceding gene: 148242802

Following gene: 148242804

Centisome position: 66.88

GC content: 61.83

Gene sequence:

>930_bases
ATGACCGCAAGCCCTCTCACAGCAGCTGAAAACAGCCGCAAGCGGGGCAATGACCCCGTCAGTTGGTACTTATCGAGTAT
CGGCCGGGTTCCATTGCTGACTCCTGCGGAGGAGATCGAGCTGGGGAACCAGGTTCAAACCATGATGACCCTGGTGGAGC
AAGGCGAGGAAGCCACCTACACCGCCCGCGAAAAGAAACTGATTCGTGTTGGCCGCCGCTCCAAGGAGCGCATGATGAAG
GCCAATCTGCGTCTGGTGGTGAGCGTTGCCAAGAAGTACCAAGGCAAAGGTTTGGAGCTGTTGGATCTGATCCAGGAGGG
TTCCTTGGGCCTGGAACGGGCCGTTGAGAAGTTCGACCCCACCCGTGGCTACAAATTCTCGACCTATGCCTTCTGGTGGA
TCCGCCAGAGCATGACCCGGGCGATCGCCTGCCAGTCCCGCACGATCCGCCTGCCGGTTCACCTCAGCGAGCGCTTGGCC
ACGGTGCGCAAAGCAACGATGGACTTGGCTCACAAATTGGGCGCCATGCCCAGCCGCAGAGAGATTGCCACCGAACTGGG
CATGGGCCTTGAAGAGCTCGATGGCCTGCTGCGTCAAGCGCTGACCACCTCCAGCCTCGATGCCCCCATCAGCGGCGAGG
AAGGCCGCAGCTTCCTGGGTGAGCTGATCGCTGATCCCCGCAATGAGGAGCCCCTCGATGCGGTGGAGCGGCGCATGCAC
CACGAGCAACTGGGCCGCTGGCTCAGCTACCTCACCGCTCAAGAAAAGCAGGTGCTGGAAATGCGCTTTGGTCTGGGGGG
CGAGCAGCGCCACACCCTCGCTGAGATCGGCCGCTTGATGGAGGTCTCCCGCGAGCGGGTGCGTCAGGTGGAACTCAAAG
CCCTGCGCAAACTCCGTAACCTCACCCGGCGCGTTCCAACCCAGCTCTGA

Upstream 100 bases:

>100_bases
AATTGTGTTGATTTCCTCCCCTAAATCTAAAAGGTTACTGAAATCACAACTGAATCAAGAAAGCCCATAGGAGGCTCTGT
TTCCGGAGCAATCCCTCCCC

Downstream 100 bases:

>100_bases
GCATCCGGGTTCCCCTCGTTTACCACCCGCTCTACAGCGCTCCACTGCCCTCCAGCCATCGCTTCCCGATGGCTAAGTTC
CGGCAGCTGCGCCAGTGCCT

Product: RNA polymerase sigma factor, sigma-70 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTASPLTAAENSRKRGNDPVSWYLSSIGRVPLLTPAEEIELGNQVQTMMTLVEQGEEATYTAREKKLIRVGRRSKERMMK
ANLRLVVSVAKKYQGKGLELLDLIQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLA
TVRKATMDLAHKLGAMPSRREIATELGMGLEELDGLLRQALTTSSLDAPISGEEGRSFLGELIADPRNEEPLDAVERRMH
HEQLGRWLSYLTAQEKQVLEMRFGLGGEQRHTLAEIGRLMEVSRERVRQVELKALRKLRNLTRRVPTQL

Sequences:

>Translated_309_residues
MTASPLTAAENSRKRGNDPVSWYLSSIGRVPLLTPAEEIELGNQVQTMMTLVEQGEEATYTAREKKLIRVGRRSKERMMK
ANLRLVVSVAKKYQGKGLELLDLIQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLA
TVRKATMDLAHKLGAMPSRREIATELGMGLEELDGLLRQALTTSSLDAPISGEEGRSFLGELIADPRNEEPLDAVERRMH
HEQLGRWLSYLTAQEKQVLEMRFGLGGEQRHTLAEIGRLMEVSRERVRQVELKALRKLRNLTRRVPTQL
>Mature_308_residues
TASPLTAAENSRKRGNDPVSWYLSSIGRVPLLTPAEEIELGNQVQTMMTLVEQGEEATYTAREKKLIRVGRRSKERMMKA
NLRLVVSVAKKYQGKGLELLDLIQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLAT
VRKATMDLAHKLGAMPSRREIATELGMGLEELDGLLRQALTTSSLDAPISGEEGRSFLGELIADPRNEEPLDAVERRMHH
EQLGRWLSYLTAQEKQVLEMRFGLGGEQRHTLAEIGRLMEVSRERVRQVELKALRKLRNLTRRVPTQL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=284, Percent_Identity=40.8450704225352, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1789448, Length=233, Percent_Identity=45.9227467811159, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1789871, Length=232, Percent_Identity=26.7241379310345, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788231, Length=207, Percent_Identity=28.5024154589372, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35150; Mature: 35019

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASPLTAAENSRKRGNDPVSWYLSSIGRVPLLTPAEEIELGNQVQTMMTLVEQGEEATY
CCCCCCCHHHCCHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHH
TAREKKLIRVGRRSKERMMKANLRLVVSVAKKYQGKGLELLDLIQEGSLGLERAVEKFDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCC
TRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLATVRKATMDLAHKLGAMPSRR
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EIATELGMGLEELDGLLRQALTTSSLDAPISGEEGRSFLGELIADPRNEEPLDAVERRMH
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
HEQLGRWLSYLTAQEKQVLEMRFGLGGEQRHTLAEIGRLMEVSRERVRQVELKALRKLRN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTRRVPTQL
HHHHCCCCC
>Mature Secondary Structure 
TASPLTAAENSRKRGNDPVSWYLSSIGRVPLLTPAEEIELGNQVQTMMTLVEQGEEATY
CCCCCCHHHCCHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHH
TAREKKLIRVGRRSKERMMKANLRLVVSVAKKYQGKGLELLDLIQEGSLGLERAVEKFDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCC
TRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLATVRKATMDLAHKLGAMPSRR
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EIATELGMGLEELDGLLRQALTTSSLDAPISGEEGRSFLGELIADPRNEEPLDAVERRMH
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
HEQLGRWLSYLTAQEKQVLEMRFGLGGEQRHTLAEIGRLMEVSRERVRQVELKALRKLRN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTRRVPTQL
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA