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The map label for this gene is ispG [H]

Identifier: 148242561

GI number: 148242561

Start: 1298751

End: 1299983

Strand: Direct

Name: ispG [H]

Synonym: SynRCC307_1462

Alternate gene names: 148242561

Gene position: 1298751-1299983 (Clockwise)

Preceding gene: 148242560

Following gene: 148242562

Centisome position: 58.37

GC content: 62.85

Gene sequence:

>1233_bases
ATGACCGGCACCCTGGCCCCCACCACCTTGAGCGACACCGATTGGAGCCAGGATCCGCGCTTTGACACGGTGATTCACCG
GCGCAAAACCCGCAGTGTGCGGGTGGGCGACATCTGGATCGGCAGTGAGCACCCGGTGGTGGTGCAATCGATGATCAACG
AGGACACCCTCGATGTGGAGGGGGCCACCGCCGGCATCCTGCGGCTGCATCAAGCAGGCTGCGAAATCGTTCGGCTTACG
GTGCCGAGCCTCGCCCACGCCAAGGCCGTCGGTGAGATCCGTCAGCGCCTCGAAGCCGCTGGGGCGAGCGTGCCGTTGGT
GGCCGATGTGCACCACAACGGCATGAAGATCGCCTTGGAGGTGGCCAAGCATGTCGACAAGGTGCGCATCAACCCTGGGC
TGTTTGTCTTCGACAAGCCCGATCCCAACCGCACCGAGTTCACACCGGACGAGGTGGCTGCCATTGGGGAGCGGATTACT
GAAACCTTTGAACCCCTCGTCCGCAGCCTGAAGGAGCAGGACAAGGCCCTGCGGATTGGTGTCAACCATGGGTCCTTGGC
TGAGCGCATGTTGTTCAGTTACGGCGATACCCCGCTGGGGATGGTGGAGTCAGCCCTGGAGTTCATTCGCATCTGCGATC
GACTCGACTTCCACAACATCGTGATTTCGATGAAGGCCTCACGGGCGCCGGTGATGCTGGCGGCCTACCGCTTGATGGCC
CACCGCATGGATGCCGAGGGGTTCAACTACCCCCTGCACCTGGGGGTCACCGAAGCAGGCGACGGTGATTACGGCCGCAT
CAAAAGCACCGCAGGGATCGCCACCTTGCTAGCCGATGGCCTGGGCGACACGATCCGCGTTTCCCTCACCGAGGCTCCCG
AGAAGGAAATTCCGGTCTGCTACGGCATCCTCCAGTCCTTGGGGCTCCGCAAAACCATGGTGGAGTACGTGGCTTGCCCC
AGCTGCGGCCGCACGCTGTTCAACCTCGAGGAGGTGTTGCAGCAAGTGCGCAGCGCCACCGACCACCTCACGGGCCTCGA
CATCGCTGTGATGGGCTGCATCGTCAACGGCCCTGGAGAAATGGCTGACGCCGACTACGGCTATGTGGGCAAAACCCCCG
GCACCATCTCGCTCTACCGGGGACGCGAAGAGATTCGCCGCGTGCCGGAGGCTGAAGGCGTGCAGGCACTGATCGATCTG
ATCAAAGAAGACGGCCGCTGGGTTGATCCCTGA

Upstream 100 bases:

>100_bases
GTGCAGCCATTTGAGCGCTGGGAGCTGCTGCACCCCGGCGAACTCGAACCCATGAGCTAATGGTCTATGCCATGGTTTTG
CGCAGACCTTGGCCCGCTCC

Downstream 100 bases:

>100_bases
AGCTGGGGCCACATCCAGTCACAGACCAGCCGGCAGGCGCTCTAACCAAAGCCCAACCGTGACGTTACGGTTGGTAGATA
CTGGCAACTTCCATGGCTTC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTGTLAPTTLSDTDWSQDPRFDTVIHRRKTRSVRVGDIWIGSEHPVVVQSMINEDTLDVEGATAGILRLHQAGCEIVRLT
VPSLAHAKAVGEIRQRLEAAGASVPLVADVHHNGMKIALEVAKHVDKVRINPGLFVFDKPDPNRTEFTPDEVAAIGERIT
ETFEPLVRSLKEQDKALRIGVNHGSLAERMLFSYGDTPLGMVESALEFIRICDRLDFHNIVISMKASRAPVMLAAYRLMA
HRMDAEGFNYPLHLGVTEAGDGDYGRIKSTAGIATLLADGLGDTIRVSLTEAPEKEIPVCYGILQSLGLRKTMVEYVACP
SCGRTLFNLEEVLQQVRSATDHLTGLDIAVMGCIVNGPGEMADADYGYVGKTPGTISLYRGREEIRRVPEAEGVQALIDL
IKEDGRWVDP

Sequences:

>Translated_410_residues
MTGTLAPTTLSDTDWSQDPRFDTVIHRRKTRSVRVGDIWIGSEHPVVVQSMINEDTLDVEGATAGILRLHQAGCEIVRLT
VPSLAHAKAVGEIRQRLEAAGASVPLVADVHHNGMKIALEVAKHVDKVRINPGLFVFDKPDPNRTEFTPDEVAAIGERIT
ETFEPLVRSLKEQDKALRIGVNHGSLAERMLFSYGDTPLGMVESALEFIRICDRLDFHNIVISMKASRAPVMLAAYRLMA
HRMDAEGFNYPLHLGVTEAGDGDYGRIKSTAGIATLLADGLGDTIRVSLTEAPEKEIPVCYGILQSLGLRKTMVEYVACP
SCGRTLFNLEEVLQQVRSATDHLTGLDIAVMGCIVNGPGEMADADYGYVGKTPGTISLYRGREEIRRVPEAEGVQALIDL
IKEDGRWVDP
>Mature_409_residues
TGTLAPTTLSDTDWSQDPRFDTVIHRRKTRSVRVGDIWIGSEHPVVVQSMINEDTLDVEGATAGILRLHQAGCEIVRLTV
PSLAHAKAVGEIRQRLEAAGASVPLVADVHHNGMKIALEVAKHVDKVRINPGLFVFDKPDPNRTEFTPDEVAAIGERITE
TFEPLVRSLKEQDKALRIGVNHGSLAERMLFSYGDTPLGMVESALEFIRICDRLDFHNIVISMKASRAPVMLAAYRLMAH
RMDAEGFNYPLHLGVTEAGDGDYGRIKSTAGIATLLADGLGDTIRVSLTEAPEKEIPVCYGILQSLGLRKTMVEYVACPS
CGRTLFNLEEVLQQVRSATDHLTGLDIAVMGCIVNGPGEMADADYGYVGKTPGTISLYRGREEIRRVPEAEGVQALIDLI
KEDGRWVDP

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=349, Percent_Identity=37.8223495702006, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44875; Mature: 44744

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTLAPTTLSDTDWSQDPRFDTVIHRRKTRSVRVGDIWIGSEHPVVVQSMINEDTLDVE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHCCCCCCCC
GATAGILRLHQAGCEIVRLTVPSLAHAKAVGEIRQRLEAAGASVPLVADVHHNGMKIALE
CCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHH
VAKHVDKVRINPGLFVFDKPDPNRTEFTPDEVAAIGERITETFEPLVRSLKEQDKALRIG
HHHHHHHEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VNHGSLAERMLFSYGDTPLGMVESALEFIRICDRLDFHNIVISMKASRAPVMLAAYRLMA
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
HRMDAEGFNYPLHLGVTEAGDGDYGRIKSTAGIATLLADGLGDTIRVSLTEAPEKEIPVC
HHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHH
YGILQSLGLRKTMVEYVACPSCGRTLFNLEEVLQQVRSATDHLTGLDIAVMGCIVNGPGE
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
MADADYGYVGKTPGTISLYRGREEIRRVPEAEGVQALIDLIKEDGRWVDP
CCCCCCCCCCCCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TGTLAPTTLSDTDWSQDPRFDTVIHRRKTRSVRVGDIWIGSEHPVVVQSMINEDTLDVE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHCCCCCCCC
GATAGILRLHQAGCEIVRLTVPSLAHAKAVGEIRQRLEAAGASVPLVADVHHNGMKIALE
CCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHH
VAKHVDKVRINPGLFVFDKPDPNRTEFTPDEVAAIGERITETFEPLVRSLKEQDKALRIG
HHHHHHHEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VNHGSLAERMLFSYGDTPLGMVESALEFIRICDRLDFHNIVISMKASRAPVMLAAYRLMA
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
HRMDAEGFNYPLHLGVTEAGDGDYGRIKSTAGIATLLADGLGDTIRVSLTEAPEKEIPVC
HHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHH
YGILQSLGLRKTMVEYVACPSCGRTLFNLEEVLQQVRSATDHLTGLDIAVMGCIVNGPGE
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
MADADYGYVGKTPGTISLYRGREEIRRVPEAEGVQALIDLIKEDGRWVDP
CCCCCCCCCCCCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]