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The map label for this gene is amiC [H]

Identifier: 148242256

GI number: 148242256

Start: 1023627

End: 1024715

Strand: Direct

Name: amiC [H]

Synonym: SynRCC307_1157

Alternate gene names: 148242256

Gene position: 1023627-1024715 (Clockwise)

Preceding gene: 148242255

Following gene: 148242257

Centisome position: 46.01

GC content: 62.35

Gene sequence:

>1089_bases
ATGTTTGAGGCTCGCCGTTGGGCTTCCAGCCTGATGGCGGGAGTTCTGGCTGCTCAGCTCATCCCCGTGCTGCCGGCCCG
TGCCTTCAGCCGTCTTGCGGCTTGGGCCATCACGGCCAAGGGTGAACTGCTGCTGCGCACCTCTCCAAACCTTGATCTCG
ACGCGTTTTTCAAAGACGGCGATGGCCGCTATGGCGATCGCATTTGGATTGATTTCCCTGGCAGCCCCCAGCGGCCGCGA
AAGATCCCAGGCAAAGGCGCCGTTCGCGAAATTCGTGTGGGTATGCCCGAGCGCGGTGTGACGCGCCTGGTGATCGAGTT
CAAGCGTGGCGTTGAACTCGACCCATCGAAGCTGCGTTTGGTGGGCCTCGACTGGGACAGCTGGCGAATGCGCTTCCCGC
TCCAGGACGCCCGCTTTGTGGCCATGGGTGAAGGCATCCTCGAGCGGGAGGTGCCCTTCCCCAGCGGGCCAACGATGCCC
GGACTCTCGAGGGCCCCACTGCGAACTCTCACGGTGGCGAGTCTGCCGTGGGTTCGGCGCGGGGCCTACCGGGTGATGCT
TGATCCTGGCCATGGGGGCAAAGACCCGGGCGCTATCGGCATTGGTGGCACCCGCGAAAAAGATGTGGTGCTGGATGTCT
CGCGGCAGGTGGCCTCACATCTGCGCAGCAAAGGTGTGGTCGTGAAGATGACGCGCAGATCAGACGCCACGGTCGATTTG
CCTCCCCGCGCCCGGCTATCGAACCAATGGCGCCCTGATGCCTTTGTGAGCATCCACGCCAATGCGATCAGCATGCGCAG
GCCGGAGGTCAACGGACTCGAGACGTTCTATTTCCAAAGCTTTCAGGGAAAAAAACTCTCCTCCTTGATTCATGACTCAA
TCCTGAGGACAGTCAGGCGCAAAGACCGAGGGGTCAAGGAGGCGCGGTTCTACGTGATCCGCCGCACCAACAGCCCCGCG
AGCCTTGTGGAGCTCGGTTTCCTTACCGGAACCGCCGATGCTGCCGATCTGGCTGACCCTGCCCACCGTCGTCAGCTGGC
ACTGGCGATCGCCGTGGGAATCCTCAACTATCTGAGAGTGGCCGGATGA

Upstream 100 bases:

>100_bases
GTGTGGCCATCGCCCCGATCGACACCGACCATCTGCAGCGCATTCGCGCGCAGATGCCGAGCCTGCGTCACCGTCGCCCA
GCCCTGTTCTGAGCCCGCCC

Downstream 100 bases:

>100_bases
CCCTGAGCTCTTCTGCTGATGCCCTGAGCCTCTCGTTGCAGCGCGTGGCCACATCGGAGTTCACCGGGTTGCGGCCTCAG
GCCCTGTTGGGATTGTTCGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MFEARRWASSLMAGVLAAQLIPVLPARAFSRLAAWAITAKGELLLRTSPNLDLDAFFKDGDGRYGDRIWIDFPGSPQRPR
KIPGKGAVREIRVGMPERGVTRLVIEFKRGVELDPSKLRLVGLDWDSWRMRFPLQDARFVAMGEGILEREVPFPSGPTMP
GLSRAPLRTLTVASLPWVRRGAYRVMLDPGHGGKDPGAIGIGGTREKDVVLDVSRQVASHLRSKGVVVKMTRRSDATVDL
PPRARLSNQWRPDAFVSIHANAISMRRPEVNGLETFYFQSFQGKKLSSLIHDSILRTVRRKDRGVKEARFYVIRRTNSPA
SLVELGFLTGTADAADLADPAHRRQLALAIAVGILNYLRVAG

Sequences:

>Translated_362_residues
MFEARRWASSLMAGVLAAQLIPVLPARAFSRLAAWAITAKGELLLRTSPNLDLDAFFKDGDGRYGDRIWIDFPGSPQRPR
KIPGKGAVREIRVGMPERGVTRLVIEFKRGVELDPSKLRLVGLDWDSWRMRFPLQDARFVAMGEGILEREVPFPSGPTMP
GLSRAPLRTLTVASLPWVRRGAYRVMLDPGHGGKDPGAIGIGGTREKDVVLDVSRQVASHLRSKGVVVKMTRRSDATVDL
PPRARLSNQWRPDAFVSIHANAISMRRPEVNGLETFYFQSFQGKKLSSLIHDSILRTVRRKDRGVKEARFYVIRRTNSPA
SLVELGFLTGTADAADLADPAHRRQLALAIAVGILNYLRVAG
>Mature_362_residues
MFEARRWASSLMAGVLAAQLIPVLPARAFSRLAAWAITAKGELLLRTSPNLDLDAFFKDGDGRYGDRIWIDFPGSPQRPR
KIPGKGAVREIRVGMPERGVTRLVIEFKRGVELDPSKLRLVGLDWDSWRMRFPLQDARFVAMGEGILEREVPFPSGPTMP
GLSRAPLRTLTVASLPWVRRGAYRVMLDPGHGGKDPGAIGIGGTREKDVVLDVSRQVASHLRSKGVVVKMTRRSDATVDL
PPRARLSNQWRPDAFVSIHANAISMRRPEVNGLETFYFQSFQGKKLSSLIHDSILRTVRRKDRGVKEARFYVIRRTNSPA
SLVELGFLTGTADAADLADPAHRRQLALAIAVGILNYLRVAG

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=255, Percent_Identity=28.2352941176471, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082163, Length=327, Percent_Identity=29.6636085626911, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790611, Length=351, Percent_Identity=25.3561253561254, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 40160; Mature: 40160

Theoretical pI: Translated: 11.54; Mature: 11.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEARRWASSLMAGVLAAQLIPVLPARAFSRLAAWAITAKGELLLRTSPNLDLDAFFKDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCCEEEEEECC
DGRYGDRIWIDFPGSPQRPRKIPGKGAVREIRVGMPERGVTRLVIEFKRGVELDPSKLRL
CCCCCCEEEEECCCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHHHCCCCCCCCCEEE
VGLDWDSWRMRFPLQDARFVAMGEGILEREVPFPSGPTMPGLSRAPLRTLTVASLPWVRR
EEECCCCCEEECCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHC
GAYRVMLDPGHGGKDPGAIGIGGTREKDVVLDVSRQVASHLRSKGVVVKMTRRSDATVDL
CCEEEEECCCCCCCCCCEECCCCCCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCEEEC
PPRARLSNQWRPDAFVSIHANAISMRRPEVNGLETFYFQSFQGKKLSSLIHDSILRTVRR
CCCHHCCCCCCCCEEEEEEECEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
KDRGVKEARFYVIRRTNSPASLVELGFLTGTADAADLADPAHRRQLALAIAVGILNYLRV
HHCCCCEEEEEEEEECCCCHHHHEEHEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHH
AG
CC
>Mature Secondary Structure
MFEARRWASSLMAGVLAAQLIPVLPARAFSRLAAWAITAKGELLLRTSPNLDLDAFFKDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCCEEEEEECC
DGRYGDRIWIDFPGSPQRPRKIPGKGAVREIRVGMPERGVTRLVIEFKRGVELDPSKLRL
CCCCCCEEEEECCCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHHHCCCCCCCCCEEE
VGLDWDSWRMRFPLQDARFVAMGEGILEREVPFPSGPTMPGLSRAPLRTLTVASLPWVRR
EEECCCCCEEECCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHC
GAYRVMLDPGHGGKDPGAIGIGGTREKDVVLDVSRQVASHLRSKGVVVKMTRRSDATVDL
CCEEEEECCCCCCCCCCEECCCCCCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCEEEC
PPRARLSNQWRPDAFVSIHANAISMRRPEVNGLETFYFQSFQGKKLSSLIHDSILRTVRR
CCCHHCCCCCCCCEEEEEEECEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
KDRGVKEARFYVIRRTNSPASLVELGFLTGTADAADLADPAHRRQLALAIAVGILNYLRV
HHCCCCEEEEEEEEECCCCHHHHEEHEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHH
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]