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The map label for this gene is yafV [C]

Identifier: 148242255

GI number: 148242255

Start: 1022800

End: 1023618

Strand: Direct

Name: yafV [C]

Synonym: SynRCC307_1156

Alternate gene names: 148242255

Gene position: 1022800-1023618 (Clockwise)

Preceding gene: 148242254

Following gene: 148242256

Centisome position: 45.97

GC content: 62.15

Gene sequence:

>819_bases
GTGAAGAGCTTTCTGGCTGCGGCGTTGCAGTGCACGAGCACCCCTGATCCAGATTCGAATTTTGCCCAGATTGAGGAGCT
CATTGATCTGGCGGCCCGTCGCGGGGCTGAGTTGGTTGGTCTGCCCGAAAATTTCGCCTTCTTAGGTGCTGAGAACAAAC
GTCTCGAGCTGGCACCAACCCTGGCTGAGCGCTGCAGCCGCTTTCTGGTGACCATGGCGCGTCGCTACCAAGTGGCGATC
CTTGGCGGTGGTTTCCCGGTTCCAGCCGGTGAAGCCGCCACGTTCAATCGCTCCGAGCTGGTGAGCCGCGATGGTCAGGT
GCTGGCCACCTACGACAAGATCCACCTCTTCGATGTCGATCTGCCCGACGGCAACACCTACACCGAATCAGACACGATCA
CAGCTGGGAGTTCCTTGCCACCGGTGGCAGACCTTCCAGGGCTCTGCCGCGTTGGTCTCTCCATTTGCTACGACGTTCGC
TTCCCCGAGCTCTACCGCCATTTGGCAGGAGCCGGCGCTGAGTTGTTGATGGTGCCGGCCGCCTTTACGGCCTACACCGG
CAAAGACCACTGGCAGGTGCTACTTCAAGCCCGTGCCATCGAAAACACCAGCTATGTGCTGGCGCCGGCGCAAACCGGGC
TGCACTATGGGCGCCGACAGAGTCACGGCCATGCGCTGGTCATTGATCCCTGGGGCACCGTTCTCGCCGATGCAGGCGTG
GGCCCTGGTGTGGCCATCGCCCCGATCGACACCGACCATCTGCAGCGCATTCGCGCGCAGATGCCGAGCCTGCGTCACCG
TCGCCCAGCCCTGTTCTGA

Upstream 100 bases:

>100_bases
CTGCGCGACGGTTGACAGCCTCACGGTGGTTCCGAGCCAGGACGAACCGGGTGTTTTGCGGTTGAGCTGATTACAGTCAT
TAGCGATTGGGTGATCTGTG

Downstream 100 bases:

>100_bases
GCCCGCCCATGTTTGAGGCTCGCCGTTGGGCTTCCAGCCTGATGGCGGGAGTTCTGGCTGCTCAGCTCATCCCCGTGCTG
CCGGCCCGTGCCTTCAGCCG

Product: nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKSFLAAALQCTSTPDPDSNFAQIEELIDLAARRGAELVGLPENFAFLGAENKRLELAPTLAERCSRFLVTMARRYQVAI
LGGGFPVPAGEAATFNRSELVSRDGQVLATYDKIHLFDVDLPDGNTYTESDTITAGSSLPPVADLPGLCRVGLSICYDVR
FPELYRHLAGAGAELLMVPAAFTAYTGKDHWQVLLQARAIENTSYVLAPAQTGLHYGRRQSHGHALVIDPWGTVLADAGV
GPGVAIAPIDTDHLQRIRAQMPSLRHRRPALF

Sequences:

>Translated_272_residues
MKSFLAAALQCTSTPDPDSNFAQIEELIDLAARRGAELVGLPENFAFLGAENKRLELAPTLAERCSRFLVTMARRYQVAI
LGGGFPVPAGEAATFNRSELVSRDGQVLATYDKIHLFDVDLPDGNTYTESDTITAGSSLPPVADLPGLCRVGLSICYDVR
FPELYRHLAGAGAELLMVPAAFTAYTGKDHWQVLLQARAIENTSYVLAPAQTGLHYGRRQSHGHALVIDPWGTVLADAGV
GPGVAIAPIDTDHLQRIRAQMPSLRHRRPALF
>Mature_272_residues
MKSFLAAALQCTSTPDPDSNFAQIEELIDLAARRGAELVGLPENFAFLGAENKRLELAPTLAERCSRFLVTMARRYQVAI
LGGGFPVPAGEAATFNRSELVSRDGQVLATYDKIHLFDVDLPDGNTYTESDTITAGSSLPPVADLPGLCRVGLSICYDVR
FPELYRHLAGAGAELLMVPAAFTAYTGKDHWQVLLQARAIENTSYVLAPAQTGLHYGRRQSHGHALVIDPWGTVLADAGV
GPGVAIAPIDTDHLQRIRAQMPSLRHRRPALF

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=274, Percent_Identity=42.7007299270073, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI297632350, Length=274, Percent_Identity=42.7007299270073, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI5031947, Length=274, Percent_Identity=42.7007299270073, Blast_Score=207, Evalue=6e-54,
Organism=Homo sapiens, GI9910460, Length=272, Percent_Identity=35.2941176470588, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI297632346, Length=194, Percent_Identity=39.1752577319588, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17556280, Length=271, Percent_Identity=40.2214022140221, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322335, Length=303, Percent_Identity=33.6633663366337, Blast_Score=172, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323383, Length=268, Percent_Identity=30.9701492537313, Blast_Score=142, Evalue=5e-35,
Organism=Drosophila melanogaster, GI17933642, Length=266, Percent_Identity=37.9699248120301, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI21355835, Length=269, Percent_Identity=32.3420074349442, Blast_Score=147, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29448; Mature: 29448

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFLAAALQCTSTPDPDSNFAQIEELIDLAARRGAELVGLPENFAFLGAENKRLELAPT
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCEEEECHH
LAERCSRFLVTMARRYQVAILGGGFPVPAGEAATFNRSELVSRDGQVLATYDKIHLFDVD
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEEEEEEEE
LPDGNTYTESDTITAGSSLPPVADLPGLCRVGLSICYDVRFPELYRHLAGAGAELLMVPA
CCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECH
AFTAYTGKDHWQVLLQARAIENTSYVLAPAQTGLHYGRRQSHGHALVIDPWGTVLADAGV
HHEECCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCCEEEECCCHHHHCCCCC
GPGVAIAPIDTDHLQRIRAQMPSLRHRRPALF
CCCEEEECCCHHHHHHHHHHCCHHHCCCCCCC
>Mature Secondary Structure
MKSFLAAALQCTSTPDPDSNFAQIEELIDLAARRGAELVGLPENFAFLGAENKRLELAPT
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCEEEECHH
LAERCSRFLVTMARRYQVAILGGGFPVPAGEAATFNRSELVSRDGQVLATYDKIHLFDVD
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEEEEEEEE
LPDGNTYTESDTITAGSSLPPVADLPGLCRVGLSICYDVRFPELYRHLAGAGAELLMVPA
CCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECH
AFTAYTGKDHWQVLLQARAIENTSYVLAPAQTGLHYGRRQSHGHALVIDPWGTVLADAGV
HHEECCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCCEEEECCCHHHHCCCCC
GPGVAIAPIDTDHLQRIRAQMPSLRHRRPALF
CCCEEEECCCHHHHHHHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]