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The map label for this gene is menD

Identifier: 148242239

GI number: 148242239

Start: 1005947

End: 1007671

Strand: Direct

Name: menD

Synonym: SynRCC307_1140

Alternate gene names: 148242239

Gene position: 1005947-1007671 (Clockwise)

Preceding gene: 148242236

Following gene: 148242240

Centisome position: 45.21

GC content: 65.97

Gene sequence:

>1725_bases
TTGAGCATCGAGGCTGCTGAGAATCTCCGCGCCGCTGAAGCCCTGCTGTCGGCTTTGATGGGGTTGGGGCTCCAACGGGT
TGTGCTGTGCCCAGGAAGCCGCAGCGGTCCGTTGGCCCTGGCCGCCTCGCGCTTGGAATCCCATGGCCTGGCGCTGACAA
CAGGCATTGATGAGCGCTCAGCTGGCTTTTTTGCCTTGGGCCAAACCCGCGCCGATGGCCAACCCACCGCTGTGATCACC
ACCTCTGGAACGGCGGTGGCCAATCTGTTGCCGGCAGCGGTGGAGGCTGATTTCAGCGCTTTGCCGCTGCTCTTGCTCAC
GGCTGATCGCCCCGAGCGACTGAAGGCCTGCGGCGCCAATCAAACGGTGAACCAGGAGGCCTATTTGCAGCCGGCCTGCC
GTGCCCTGCTTCAAGGGCCCGCTGCGGGGTTGCATGACGCCAGTGATGAGCGTTTGCTGCGCCTAGCTCAGTCGGCCATG
CGCCACGCCCTCGGTGCGCCTGCAGGGCCGGTGCACCTCAACCTGGCCTTTGATGAGCCGCTGCATGCCGATCTCAGCGG
GCTGCCAGCTGCTGCTCCGCCGGCAGCGCAAGAGCTGCCCCCCTTGTTTGAGCCGCCAGGGCTGGGGAGCTTGGAGCCTT
TGGATCCCGATCAGCCCGGGGTGATTGTGGTGGGCCCTTGGCGGCGCGGCCATGGCGGCCGCTTTGTTGCCGCGTTGCAG
CAGCTCAACCGGCGCACGGGCTGGCCGATCCTGGCGGATGCCTCCTCCGGGCTTCGGGGCCTGCCTTTGCCGCTCGTCAG
CGGTTACGACCTGCTGCTGGCTGAGCCGCAGCGGTTGCCGCCAGCCCATCAGGTGTTGCGCTTGGGCTCGATGCCTGCCA
GCCGCCGCCTGCAGCAATGGCTGCAGACCTTTGAAGGGCCCCAGCTCGTGATCACCGAAGCCGAACCGAGGCGGCAGGAC
CCCTTGGCCAGTGGTTGCGCCCAGCATCCCCATGGGTTGGCTGCTTGGGTGGAACTGCTGCCTACAGGTGAGCCGTCGGC
CACCAGCCGCGCCGATGCCGCTCGCTGGCAACAAGCGGAATCCTTGCTGCAACAGCAACTCGATCACGACCTCCCCCTGC
AGGGCCCCTGCAGCGAACCGGCCTTGGCCCGGGCCCTGCAGAACCTGATTCCTTCGGAGTGGCCGGTGATGTTGGCCAGC
AGCTCACCGGTGAGGGATTGGGAAAGCTTTGCCGGGCGCCAGAGCGGCCACCGCACGATCGTGAGCTTTCGGGGCGCCTC
GGGCATTGATGGCACTCTCTCACTGGCCGCTGGGATCGCGCAGCAGTGGCAGCGACTGGTGCTGGTCACCGGTGATTTGG
CCCTGCTGCATGACGCCAACGGTTGGCTCTGGCGCCGTCAGCTTTCTGGAGAGCTGCGCCTTGTTGTGATCGACAACGGC
GGCGGTGGCGTCTTTGAGCAGTTGCCAATCCCTAGGCAGTCGATGGATTTTGATCGTCTCTTCGCCATGCCCCAGAGCTG
TGATGCCATGGCCCTCGCGGCAGCACACGGTGTGGCGGCCCGTGACTGCAGTGCCATGGAGACCTTGGCCGCCGATCTGC
AGTGGTTGCTTGAGCCTGGTGATGCGATGCGCCTGCTGCGATGCTCCACCGACCGGTCCCGTGATGCCCAGCTGCGCCAG
CAGTTGCGTGACAAGCCATGGTGGGAGCAGACACCAGCTCCTTGA

Upstream 100 bases:

>100_bases
ACCACCAGCAAGAGCCCCTGCAGCTGCTGGCGCCATGTGTTGCGTTGTTCCATCACTGGGCGATCGGCGAGCACAATCGT
GACGCGCCACCAGGCCCCCG

Downstream 100 bases:

>100_bases
TGGCTGTGCCCGCCTACCCATGGATCCGTCAGGGCAACTACGACGACGTGCTCTTCGAGCTGTCGGCTGATGAGGGGATT
GCGCGCATCAGCATCAATCG

Product: menaquinone biosynthesis protein MenD (2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase)

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MSIEAAENLRAAEALLSALMGLGLQRVVLCPGSRSGPLALAASRLESHGLALTTGIDERSAGFFALGQTRADGQPTAVIT
TSGTAVANLLPAAVEADFSALPLLLLTADRPERLKACGANQTVNQEAYLQPACRALLQGPAAGLHDASDERLLRLAQSAM
RHALGAPAGPVHLNLAFDEPLHADLSGLPAAAPPAAQELPPLFEPPGLGSLEPLDPDQPGVIVVGPWRRGHGGRFVAALQ
QLNRRTGWPILADASSGLRGLPLPLVSGYDLLLAEPQRLPPAHQVLRLGSMPASRRLQQWLQTFEGPQLVITEAEPRRQD
PLASGCAQHPHGLAAWVELLPTGEPSATSRADAARWQQAESLLQQQLDHDLPLQGPCSEPALARALQNLIPSEWPVMLAS
SSPVRDWESFAGRQSGHRTIVSFRGASGIDGTLSLAAGIAQQWQRLVLVTGDLALLHDANGWLWRRQLSGELRLVVIDNG
GGGVFEQLPIPRQSMDFDRLFAMPQSCDAMALAAAHGVAARDCSAMETLAADLQWLLEPGDAMRLLRCSTDRSRDAQLRQ
QLRDKPWWEQTPAP

Sequences:

>Translated_574_residues
MSIEAAENLRAAEALLSALMGLGLQRVVLCPGSRSGPLALAASRLESHGLALTTGIDERSAGFFALGQTRADGQPTAVIT
TSGTAVANLLPAAVEADFSALPLLLLTADRPERLKACGANQTVNQEAYLQPACRALLQGPAAGLHDASDERLLRLAQSAM
RHALGAPAGPVHLNLAFDEPLHADLSGLPAAAPPAAQELPPLFEPPGLGSLEPLDPDQPGVIVVGPWRRGHGGRFVAALQ
QLNRRTGWPILADASSGLRGLPLPLVSGYDLLLAEPQRLPPAHQVLRLGSMPASRRLQQWLQTFEGPQLVITEAEPRRQD
PLASGCAQHPHGLAAWVELLPTGEPSATSRADAARWQQAESLLQQQLDHDLPLQGPCSEPALARALQNLIPSEWPVMLAS
SSPVRDWESFAGRQSGHRTIVSFRGASGIDGTLSLAAGIAQQWQRLVLVTGDLALLHDANGWLWRRQLSGELRLVVIDNG
GGGVFEQLPIPRQSMDFDRLFAMPQSCDAMALAAAHGVAARDCSAMETLAADLQWLLEPGDAMRLLRCSTDRSRDAQLRQ
QLRDKPWWEQTPAP
>Mature_573_residues
SIEAAENLRAAEALLSALMGLGLQRVVLCPGSRSGPLALAASRLESHGLALTTGIDERSAGFFALGQTRADGQPTAVITT
SGTAVANLLPAAVEADFSALPLLLLTADRPERLKACGANQTVNQEAYLQPACRALLQGPAAGLHDASDERLLRLAQSAMR
HALGAPAGPVHLNLAFDEPLHADLSGLPAAAPPAAQELPPLFEPPGLGSLEPLDPDQPGVIVVGPWRRGHGGRFVAALQQ
LNRRTGWPILADASSGLRGLPLPLVSGYDLLLAEPQRLPPAHQVLRLGSMPASRRLQQWLQTFEGPQLVITEAEPRRQDP
LASGCAQHPHGLAAWVELLPTGEPSATSRADAARWQQAESLLQQQLDHDLPLQGPCSEPALARALQNLIPSEWPVMLASS
SPVRDWESFAGRQSGHRTIVSFRGASGIDGTLSLAAGIAQQWQRLVLVTGDLALLHDANGWLWRRQLSGELRLVVIDNGG
GGVFEQLPIPRQSMDFDRLFAMPQSCDAMALAAAHGVAARDCSAMETLAADLQWLLEPGDAMRLLRCSTDRSRDAQLRQQ
LRDKPWWEQTPAP

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=579, Percent_Identity=33.160621761658, Blast_Score=197, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_SYNR3 (A5GT34)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001227396.1
- ProteinModelPortal:   A5GT34
- STRING:   A5GT34
- GeneID:   5158463
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_1140
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 61353; Mature: 61221

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEAAENLRAAEALLSALMGLGLQRVVLCPGSRSGPLALAASRLESHGLALTTGIDERS
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCCC
AGFFALGQTRADGQPTAVITTSGTAVANLLPAAVEADFSALPLLLLTADRPERLKACGAN
CCEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHCCEEEEECCCHHHHHHCCCC
QTVNQEAYLQPACRALLQGPAAGLHDASDERLLRLAQSAMRHALGAPAGPVHLNLAFDEP
CCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
LHADLSGLPAAAPPAAQELPPLFEPPGLGSLEPLDPDQPGVIVVGPWRRGHGGRFVAALQ
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHH
QLNRRTGWPILADASSGLRGLPLPLVSGYDLLLAEPQRLPPAHQVLRLGSMPASRRLQQW
HHHHHCCCCEEEECCCCCCCCCCHHHCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHH
LQTFEGPQLVITEAEPRRQDPLASGCAQHPHGLAAWVELLPTGEPSATSRADAARWQQAE
HHHCCCCEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
SLLQQQLDHDLPLQGPCSEPALARALQNLIPSEWPVMLASSSPVRDWESFAGRQSGHRTI
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCCCEEE
VSFRGASGIDGTLSLAAGIAQQWQRLVLVTGDLALLHDANGWLWRRQLSGELRLVVIDNG
EEECCCCCCCHHHHHHHHHHHHHHEEEEEECCEEEEECCCCCEEEEECCCCEEEEEEECC
GGGVFEQLPIPRQSMDFDRLFAMPQSCDAMALAAAHGVAARDCSAMETLAADLQWLLEPG
CCCCHHCCCCCCCCCCHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
DAMRLLRCSTDRSRDAQLRQQLRDKPWWEQTPAP
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SIEAAENLRAAEALLSALMGLGLQRVVLCPGSRSGPLALAASRLESHGLALTTGIDERS
CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCCC
AGFFALGQTRADGQPTAVITTSGTAVANLLPAAVEADFSALPLLLLTADRPERLKACGAN
CCEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHCCEEEEECCCHHHHHHCCCC
QTVNQEAYLQPACRALLQGPAAGLHDASDERLLRLAQSAMRHALGAPAGPVHLNLAFDEP
CCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
LHADLSGLPAAAPPAAQELPPLFEPPGLGSLEPLDPDQPGVIVVGPWRRGHGGRFVAALQ
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHH
QLNRRTGWPILADASSGLRGLPLPLVSGYDLLLAEPQRLPPAHQVLRLGSMPASRRLQQW
HHHHHCCCCEEEECCCCCCCCCCHHHCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHH
LQTFEGPQLVITEAEPRRQDPLASGCAQHPHGLAAWVELLPTGEPSATSRADAARWQQAE
HHHCCCCEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
SLLQQQLDHDLPLQGPCSEPALARALQNLIPSEWPVMLASSSPVRDWESFAGRQSGHRTI
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCCCEEE
VSFRGASGIDGTLSLAAGIAQQWQRLVLVTGDLALLHDANGWLWRRQLSGELRLVVIDNG
EEECCCCCCCHHHHHHHHHHHHHHEEEEEECCEEEEECCCCCEEEEECCCCEEEEEEECC
GGGVFEQLPIPRQSMDFDRLFAMPQSCDAMALAAAHGVAARDCSAMETLAADLQWLLEPG
CCCCHHCCCCCCCCCCHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
DAMRLLRCSTDRSRDAQLRQQLRDKPWWEQTPAP
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA