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The map label for this gene is cysD [H]

Identifier: 148241894

GI number: 148241894

Start: 726006

End: 727349

Strand: Direct

Name: cysD [H]

Synonym: SynRCC307_0795

Alternate gene names: 148241894

Gene position: 726006-727349 (Clockwise)

Preceding gene: 148241893

Following gene: 148241895

Centisome position: 32.63

GC content: 58.48

Gene sequence:

>1344_bases
ATGAGTGCCCAAGCATTGATGAGCCAACGGTTTGAAACGCTTCAGCTGCACGCTGGCCAGCAGGCGGATCCCACCACCAA
TGCCCGTGCGGTTCCGATTTATCAAACCTCCTCCTACGTCTTCAATGACGCCGAGCACGGCGCCAATCTGTTCGGGCTGA
AGGAATTCGGGAACATCTACACCCGCTTGATGAACCCCACCACCGATGTCTTTGAAAAGCGGGTGGCGGCGCTGGAGGGT
GGCGTGGCTGCGGTGGCCACCGCCTCAGGGCAGTCGGCGCAATTTTTGGCCATCACCAACTTCATGCAGGCTGGTGACAA
CTTGGTCTCCACCTCCTTTTTGTATGGAGGCACCTACAACCAGTTCAAGGTTCAGTTCCCCCGGCTGGGCATTCAGGTGA
AATTCGCTGAAGGCGATGACCCGGACAGCTTCAAAGCCCAAATCGATGAGAACACCAAGGCCATCTATGTCGAGGCGATG
GGCAACCCCCGCTTCAACATCCCCGATTTCAAAGCTTTGGCTGCCTTGGCCCATGACCATGGCATTCCTCTGATCGTTGA
CAACACCCTCGGCGCCGCTGGTGCCCTGATTCGCCCCATCGAGCATGGCGCTGATGTGGTGGTGGAAAGCGCCACCAAGT
GGATTGGTGGTCATGGCACCAGCCTGGGCGGGGTGATTGTTGATGCCGGCACCTTCGATTGGGGCTCAGGCAAATTCCCT
TTGATGAGTCAGCCCAGCGCTGCCTATCACGGCCTTGTGCACTGGGATGCGTTTGGTTTTGGCAGCGATATCTGCGGCAT
GCTCGGCCTGCCGGCTGATCGCAACATCGCCTTTGCCCTGCGGGCTCGCATTGAGGGCTTGCGCGATTGGGGCCCGGCCC
AAAGCCCCTTCAACTCCTTCATGCTGTTGCAGGGGCTTGAAACCCTCAGCCTTCGGGTGGAGCGCCATGCGTCCAATGCC
ATGGCCTTGGCCACTTGGCTGCAATCTCAGCCTCAGGTGGAGAGTGTTAGCTATCCCGGCTTGGCTGGTGATCCGTATCA
CGAACGCGCTAAGACCTACTGCACCTCACGCGGCATGGGTTGCATGTTGATGTTCACCCTCAAAGGTGGATTCGACGATG
CGGTGTCGTTCATTAATGGTTTGGAATTGGCCAGTCATTTGGCCAATGTGGGTGATGCCAAAACCCTGGTGATTCACCCG
GCCTCCACCACCCACCAGCAGCTCAGCGAACACGAGCAGCGTTCGGCTGGCGTCACGCCAACCATGGTGCGCGTTTCGGT
GGGCATTGAGCACATTGATGACATCGTTGCCGACTTCAGCCAGGCCCTCGCGGGCCTGAGTTGA

Upstream 100 bases:

>100_bases
TTGCACTGCATCGAGCGCTTCAGCGATGCCGACCGCAAACGCTTCGCCCAGCGGCAGCAGCAGCTCGAGAGGGGGGATAT
CTGGAGGCTAGAAAGGGGCG

Downstream 100 bases:

>100_bases
CGACGAGGGCAAGAGACCGTGGCGCTCATTCTGCCGAAGAACTATCACAAAGTTGCGGCGATTGAGCGCAATGGGATCTC
CTGGATTGAGCCTGAATTGG

Product: O-Acetyl homoserine sulfhydrylase

Products: L-methionine; acetate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSAQALMSQRFETLQLHAGQQADPTTNARAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRVAALEG
GVAAVATASGQSAQFLAITNFMQAGDNLVSTSFLYGGTYNQFKVQFPRLGIQVKFAEGDDPDSFKAQIDENTKAIYVEAM
GNPRFNIPDFKALAALAHDHGIPLIVDNTLGAAGALIRPIEHGADVVVESATKWIGGHGTSLGGVIVDAGTFDWGSGKFP
LMSQPSAAYHGLVHWDAFGFGSDICGMLGLPADRNIAFALRARIEGLRDWGPAQSPFNSFMLLQGLETLSLRVERHASNA
MALATWLQSQPQVESVSYPGLAGDPYHERAKTYCTSRGMGCMLMFTLKGGFDDAVSFINGLELASHLANVGDAKTLVIHP
ASTTHQQLSEHEQRSAGVTPTMVRVSVGIEHIDDIVADFSQALAGLS

Sequences:

>Translated_447_residues
MSAQALMSQRFETLQLHAGQQADPTTNARAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRVAALEG
GVAAVATASGQSAQFLAITNFMQAGDNLVSTSFLYGGTYNQFKVQFPRLGIQVKFAEGDDPDSFKAQIDENTKAIYVEAM
GNPRFNIPDFKALAALAHDHGIPLIVDNTLGAAGALIRPIEHGADVVVESATKWIGGHGTSLGGVIVDAGTFDWGSGKFP
LMSQPSAAYHGLVHWDAFGFGSDICGMLGLPADRNIAFALRARIEGLRDWGPAQSPFNSFMLLQGLETLSLRVERHASNA
MALATWLQSQPQVESVSYPGLAGDPYHERAKTYCTSRGMGCMLMFTLKGGFDDAVSFINGLELASHLANVGDAKTLVIHP
ASTTHQQLSEHEQRSAGVTPTMVRVSVGIEHIDDIVADFSQALAGLS
>Mature_446_residues
SAQALMSQRFETLQLHAGQQADPTTNARAVPIYQTSSYVFNDAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRVAALEGG
VAAVATASGQSAQFLAITNFMQAGDNLVSTSFLYGGTYNQFKVQFPRLGIQVKFAEGDDPDSFKAQIDENTKAIYVEAMG
NPRFNIPDFKALAALAHDHGIPLIVDNTLGAAGALIRPIEHGADVVVESATKWIGGHGTSLGGVIVDAGTFDWGSGKFPL
MSQPSAAYHGLVHWDAFGFGSDICGMLGLPADRNIAFALRARIEGLRDWGPAQSPFNSFMLLQGLETLSLRVERHASNAM
ALATWLQSQPQVESVSYPGLAGDPYHERAKTYCTSRGMGCMLMFTLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPA
STTHQQLSEHEQRSAGVTPTMVRVSVGIEHIDDIVADFSQALAGLS

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=439, Percent_Identity=30.751708428246, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI299473759, Length=439, Percent_Identity=28.4738041002278, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI25453487, Length=438, Percent_Identity=27.6255707762557, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1790375, Length=436, Percent_Identity=30.045871559633, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789383, Length=445, Percent_Identity=22.247191011236, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71980708, Length=459, Percent_Identity=27.6688453159041, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17560056, Length=436, Percent_Identity=28.4403669724771, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17538055, Length=436, Percent_Identity=28.6697247706422, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323333, Length=452, Percent_Identity=52.8761061946903, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6319307, Length=443, Percent_Identity=30.2483069977427, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321170, Length=360, Percent_Identity=26.3888888888889, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19922554, Length=438, Percent_Identity=28.0821917808219, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.49

Molecular weight: Translated: 47944; Mature: 47812

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQALMSQRFETLQLHAGQQADPTTNARAVPIYQTSSYVFNDAEHGANLFGLKEFGNIY
CCHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEEECCCCEECCHHCCCCEEEHHHHHHHH
TRLMNPTTDVFEKRVAALEGGVAAVATASGQSAQFLAITNFMQAGDNLVSTSFLYGGTYN
HHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHCCCCCEEEEEEECCCCC
QFKVQFPRLGIQVKFAEGDDPDSFKAQIDENTKAIYVEAMGNPRFNIPDFKALAALAHDH
EEEEEECCCCEEEEECCCCCCCCHHHEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHCC
GIPLIVDNTLGAAGALIRPIEHGADVVVESATKWIGGHGTSLGGVIVDAGTFDWGSGKFP
CCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
LMSQPSAAYHGLVHWDAFGFGSDICGMLGLPADRNIAFALRARIEGLRDWGPAQSPFNSF
CCCCCCHHHCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
MLLQGLETLSLRVERHASNAMALATWLQSQPQVESVSYPGLAGDPYHERAKTYCTSRGMG
HHHHCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
CMLMFTLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTHQQLSEHEQRSAGVTP
EEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCC
TMVRVSVGIEHIDDIVADFSQALAGLS
EEEEEECCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAQALMSQRFETLQLHAGQQADPTTNARAVPIYQTSSYVFNDAEHGANLFGLKEFGNIY
CHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEEECCCCEECCHHCCCCEEEHHHHHHHH
TRLMNPTTDVFEKRVAALEGGVAAVATASGQSAQFLAITNFMQAGDNLVSTSFLYGGTYN
HHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHCCCCCEEEEEEECCCCC
QFKVQFPRLGIQVKFAEGDDPDSFKAQIDENTKAIYVEAMGNPRFNIPDFKALAALAHDH
EEEEEECCCCEEEEECCCCCCCCHHHEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHCC
GIPLIVDNTLGAAGALIRPIEHGADVVVESATKWIGGHGTSLGGVIVDAGTFDWGSGKFP
CCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
LMSQPSAAYHGLVHWDAFGFGSDICGMLGLPADRNIAFALRARIEGLRDWGPAQSPFNSF
CCCCCCHHHCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
MLLQGLETLSLRVERHASNAMALATWLQSQPQVESVSYPGLAGDPYHERAKTYCTSRGMG
HHHHCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
CMLMFTLKGGFDDAVSFINGLELASHLANVGDAKTLVIHPASTTHQQLSEHEQRSAGVTP
EEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCC
TMVRVSVGIEHIDDIVADFSQALAGLS
EEEEEECCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: O-acetyl-L-homoserine; methanethiol

Specific reaction: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]