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The map label for this gene is bioB

Identifier: 148241892

GI number: 148241892

Start: 724104

End: 725084

Strand: Direct

Name: bioB

Synonym: SynRCC307_0793

Alternate gene names: 148241892

Gene position: 724104-725084 (Clockwise)

Preceding gene: 148241891

Following gene: 148241893

Centisome position: 32.55

GC content: 62.28

Gene sequence:

>981_bases
ATGTCCGCTCTGATTCGCCACGACTGGGATCTCGCTGAAATCGAAGCCTTGCTGGAGCTGCCGTTGGTGGATCTGCTTTG
GCACGCTCAGCAGGTGCATCGCGACGCGCATCCAGCCGGTTATCACGTGCAGTTGGCGTCTTTGCTGAGCGTCAAAACCG
GTGGCTGTGAAGAGGACTGCGCCTATTGCCCGCAATCCATGCATCACAGCAGTGATGTGACGGCGCAACCGGAGCTGCAA
TTGCAAGTGGATGGTGTGCTGCAGCAGGCCCGCTCGGCACGGGATGCGGGGGCCCATCGCTTTTGCATGGGTTGGGCCTG
GCGTGAGATCCGTGAGGGCGCACCGTTTGACGCGATGCTCGCCATGGTGCGGGGTGTGCGCGAGCTGGGCATGGAAGCCT
GCGTCACCGCTGGCATGCTCACCGATTCGCAAGCCGAGCGCCTCGCCCAAGCCGGACTGACGTCGTACAACCACAACCTC
GATACCAGCCCCGAGCATTACGACCAAATCATCAGCACCCGCACCTATCAAGAGCGCCTCGACACCCTGCAGCGGGCCCG
TGATGCTGGCTTGAGCCTGTGCTGCGGCGGCATCATCGGCATGGGCGAATCACGCCGTGATCGCGCCTCCCTTTTGCAGG
TGTTGGCTGGGTTGAATCCCCATCCTGAAAGCGTTCCCATCAATGGCTTGGTAGCCGTGGAGGGAACGCCTTTGGAAGAT
CAAGAGCCCTGGGAACCGCTGGAGTTGGTGCGCATGGTGGCGGTGGCCAGGATCCTGATGCCCGAGGCCCGGGTGCGACT
CAGTGCAGGCCGGGAAAGGTTGAGCCAGGAGGCTCAGATCCTGTGCTTGCTGGCCGGTGCTGATTCGATCTTCTATGGCG
ACAGCTTGCTCACCACCAGCAACCCAGCGGTTCAGGCTGATCAGGCGCTTCTGGCTGCAGCTGGAGTGAGCAGCAGTTTG
GAGGGCGTGGCAGCGGCCTGA

Upstream 100 bases:

>100_bases
ATGTGTTGTGGCCTGATTTTGATGCGACGGCCCTGACGCAGGCCCTGGTTGATTTCCAGGGCCGTCAGCGCCGTTTTGGT
GGCGTGCAAGAGGAGATCTG

Downstream 100 bases:

>100_bases
TGGCCCCGAACGGTCCATGGCTGGTGGCGGCGTTTTATCGCTTTGCGCCGTTGCAGAACTTGGACAACCTGCGCGCGGAG
CTGCAACAGCTTGGCGGGGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSALIRHDWDLAEIEALLELPLVDLLWHAQQVHRDAHPAGYHVQLASLLSVKTGGCEEDCAYCPQSMHHSSDVTAQPELQ
LQVDGVLQQARSARDAGAHRFCMGWAWREIREGAPFDAMLAMVRGVRELGMEACVTAGMLTDSQAERLAQAGLTSYNHNL
DTSPEHYDQIISTRTYQERLDTLQRARDAGLSLCCGGIIGMGESRRDRASLLQVLAGLNPHPESVPINGLVAVEGTPLED
QEPWEPLELVRMVAVARILMPEARVRLSAGRERLSQEAQILCLLAGADSIFYGDSLLTTSNPAVQADQALLAAAGVSSSL
EGVAAA

Sequences:

>Translated_326_residues
MSALIRHDWDLAEIEALLELPLVDLLWHAQQVHRDAHPAGYHVQLASLLSVKTGGCEEDCAYCPQSMHHSSDVTAQPELQ
LQVDGVLQQARSARDAGAHRFCMGWAWREIREGAPFDAMLAMVRGVRELGMEACVTAGMLTDSQAERLAQAGLTSYNHNL
DTSPEHYDQIISTRTYQERLDTLQRARDAGLSLCCGGIIGMGESRRDRASLLQVLAGLNPHPESVPINGLVAVEGTPLED
QEPWEPLELVRMVAVARILMPEARVRLSAGRERLSQEAQILCLLAGADSIFYGDSLLTTSNPAVQADQALLAAAGVSSSL
EGVAAA
>Mature_325_residues
SALIRHDWDLAEIEALLELPLVDLLWHAQQVHRDAHPAGYHVQLASLLSVKTGGCEEDCAYCPQSMHHSSDVTAQPELQL
QVDGVLQQARSARDAGAHRFCMGWAWREIREGAPFDAMLAMVRGVRELGMEACVTAGMLTDSQAERLAQAGLTSYNHNLD
TSPEHYDQIISTRTYQERLDTLQRARDAGLSLCCGGIIGMGESRRDRASLLQVLAGLNPHPESVPINGLVAVEGTPLEDQ
EPWEPLELVRMVAVARILMPEARVRLSAGRERLSQEAQILCLLAGADSIFYGDSLLTTSNPAVQADQALLAAAGVSSSLE
GVAAA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=321, Percent_Identity=52.3364485981308, Blast_Score=337, Evalue=5e-94,
Organism=Saccharomyces cerevisiae, GI6321725, Length=317, Percent_Identity=41.0094637223975, Blast_Score=276, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_SYNR3 (A5GS37)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001227049.1
- ProteinModelPortal:   A5GS37
- SMR:   A5GS37
- STRING:   A5GS37
- GeneID:   5156357
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_0793
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35348; Mature: 35217

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALIRHDWDLAEIEALLELPLVDLLWHAQQVHRDAHPAGYHVQLASLLSVKTGGCEEDC
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
AYCPQSMHHSSDVTAQPELQLQVDGVLQQARSARDAGAHRFCMGWAWREIREGAPFDAML
HHCCHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
AMVRGVRELGMEACVTAGMLTDSQAERLAQAGLTSYNHNLDTSPEHYDQIISTRTYQERL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DTLQRARDAGLSLCCGGIIGMGESRRDRASLLQVLAGLNPHPESVPINGLVAVEGTPLED
HHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC
QEPWEPLELVRMVAVARILMPEARVRLSAGRERLSQEAQILCLLAGADSIFYGDSLLTTS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCEEECCEEEECC
NPAVQADQALLAAAGVSSSLEGVAAA
CCCHHHHHHHHHHHCCCHHCCHHCCC
>Mature Secondary Structure 
SALIRHDWDLAEIEALLELPLVDLLWHAQQVHRDAHPAGYHVQLASLLSVKTGGCEEDC
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
AYCPQSMHHSSDVTAQPELQLQVDGVLQQARSARDAGAHRFCMGWAWREIREGAPFDAML
HHCCHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
AMVRGVRELGMEACVTAGMLTDSQAERLAQAGLTSYNHNLDTSPEHYDQIISTRTYQERL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DTLQRARDAGLSLCCGGIIGMGESRRDRASLLQVLAGLNPHPESVPINGLVAVEGTPLED
HHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC
QEPWEPLELVRMVAVARILMPEARVRLSAGRERLSQEAQILCLLAGADSIFYGDSLLTTS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCEEECCEEEECC
NPAVQADQALLAAAGVSSSLEGVAAA
CCCHHHHHHHHHHHCCCHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA