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The map label for this gene is lpdA [H]

Identifier: 148241714

GI number: 148241714

Start: 548684

End: 550123

Strand: Direct

Name: lpdA [H]

Synonym: SynRCC307_0615

Alternate gene names: 148241714

Gene position: 548684-550123 (Clockwise)

Preceding gene: 148241713

Following gene: 148241715

Centisome position: 24.66

GC content: 62.36

Gene sequence:

>1440_bases
TTGACCGCGGCGATGGACTACGACCTGATCGTGATCGGGGCCGGCTATGGCGGCTTTGATGCAGCGAAGCACGCTGCTGA
GAAGGGCCTGAAGACGGCCATCATCGAGAGCCGCGAAATGGGCGGCACCTGCGTCAACCGCGGTTGTGTGCCCTCTAAAG
CGCTATTGGCTGCCAGCGGCCGGGTGCGTGAGCTCAGCGATGGTGAGCACCTCTCCTCTTTTGGCATCACGCCGGGCACC
GTTCAGTTCGATCGCCAGGCGATCGCTGATCACGCCACCCAGCTGGTGGAGAACATCCGCGCCAATCTCACCAAATCCTT
AGAGCGCGCTGGCGTCACCATCGTGCGCGGCACGGCCCAGTTGGCCGGCCCGCAGCAGGTGGCTGTGCGGCAGAGCAATG
GTGTGGAGCGGGTGCTCAGCGCCACCGATGTGCTGATTGCCACGGGCTCCGATCCCTTTGTGCCGCGCGGCATTGAAACC
GACGGACTCACGGTGTTCACCAGCGATGACGCCGTGCGCCTCGAAAGCCTGCCCCAGTGGCTGGCGATCATCGGCAGTGG
CTACATCGGCTTGGAATTTGCCGATGTCTATACAGCTTTGGGCTGTGAAGTCACGATGATCGAGGCCCTCGATCGGGTGA
TGCCCACCTTTGATCCCGACATCGCCAAATTGGCAGGCCGCAAGCTGATTGAAAGCCGCGATATCGACACCCGCTCCGGT
GTCTTTGCCTCCAAGGTGATCCCGGGCAGCCCGGTCAAGATCGAGCTGATTGATGCCGGCACCAAAGAATTGGTGGAAGT
GCTGGAAGTTGATGCGGTGCTGGTGGCCACCGGCCGAGTTCCCACCAGTGCTGATCTCAACTTGGCTGCTGTGGGGGTTG
AGAGCGAGCGCGGCTTTATTCCCGTGGATGACGGGCTGCGGGTGCTCGCTGGCGGCAATCCCGTGCCCCATCTCTGGGCC
GTTGGCGATGTGACCGGCAAGTTGATGCTGGCCCACACCGCAGCCGCCCAGGGTGTTGTGGCGGTTGAGAACATCTGCGG
CGGAAACCGCACCGTGGATTACCGCTCGATTCCAGCGGCCACCTTCACCCACCCTGAGATCAGCTCGGTCGGCCTCAGCG
AGGCCGATGCCAAAGCCATCGCTGCTGAGCAGGGCTTTGAACTGGGCAGCGTGCGCAGCTACTTCAAGGCCAACTCCAAG
GCCCTGGCGGAGCTGGAGAGCGATGGCTTGATGAAGCTGCTGTTCCGCAAAGACACCGGTGAGGTGCTCGGTGCCCACAT
CTTTGGTTTGCACGCCGCTGATCTGATTCAAGAGGTGGCCAATGCCGTGGCCCGCCGCCAGTCGGTGCGTGACCTCGTTT
ATGAGGTGCATACCCATCCCACCTTGAGTGAGGTGGTGGAATCGGCCTACAAGCAGGCCGCCCATGCCCTTGCTGCTTGA

Upstream 100 bases:

>100_bases
TGGAATCCCTCAATGTGGCCGTGGCAGCGGGCGTGCTGTTACTGGAGCGCGTAAGGCAGGGGCACGACAGGAGAGAATCG
GCGGCAACTGCGTTGGGCGC

Downstream 100 bases:

>100_bases
GCTCCGCCATGGAAATCCGTCGCCGGCCGCCCAACCCCAAGGTGCGTGTGGCCCATCTGGAATACGCCGTCCCCCACGAC
GATGAGGCCCCGCGCCACAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; LPD; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTAAMDYDLIVIGAGYGGFDAAKHAAEKGLKTAIIESREMGGTCVNRGCVPSKALLAASGRVRELSDGEHLSSFGITPGT
VQFDRQAIADHATQLVENIRANLTKSLERAGVTIVRGTAQLAGPQQVAVRQSNGVERVLSATDVLIATGSDPFVPRGIET
DGLTVFTSDDAVRLESLPQWLAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDIAKLAGRKLIESRDIDTRSG
VFASKVIPGSPVKIELIDAGTKELVEVLEVDAVLVATGRVPTSADLNLAAVGVESERGFIPVDDGLRVLAGGNPVPHLWA
VGDVTGKLMLAHTAAAQGVVAVENICGGNRTVDYRSIPAATFTHPEISSVGLSEADAKAIAAEQGFELGSVRSYFKANSK
ALAELESDGLMKLLFRKDTGEVLGAHIFGLHAADLIQEVANAVARRQSVRDLVYEVHTHPTLSEVVESAYKQAAHALAA

Sequences:

>Translated_479_residues
MTAAMDYDLIVIGAGYGGFDAAKHAAEKGLKTAIIESREMGGTCVNRGCVPSKALLAASGRVRELSDGEHLSSFGITPGT
VQFDRQAIADHATQLVENIRANLTKSLERAGVTIVRGTAQLAGPQQVAVRQSNGVERVLSATDVLIATGSDPFVPRGIET
DGLTVFTSDDAVRLESLPQWLAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDIAKLAGRKLIESRDIDTRSG
VFASKVIPGSPVKIELIDAGTKELVEVLEVDAVLVATGRVPTSADLNLAAVGVESERGFIPVDDGLRVLAGGNPVPHLWA
VGDVTGKLMLAHTAAAQGVVAVENICGGNRTVDYRSIPAATFTHPEISSVGLSEADAKAIAAEQGFELGSVRSYFKANSK
ALAELESDGLMKLLFRKDTGEVLGAHIFGLHAADLIQEVANAVARRQSVRDLVYEVHTHPTLSEVVESAYKQAAHALAA
>Mature_478_residues
TAAMDYDLIVIGAGYGGFDAAKHAAEKGLKTAIIESREMGGTCVNRGCVPSKALLAASGRVRELSDGEHLSSFGITPGTV
QFDRQAIADHATQLVENIRANLTKSLERAGVTIVRGTAQLAGPQQVAVRQSNGVERVLSATDVLIATGSDPFVPRGIETD
GLTVFTSDDAVRLESLPQWLAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDIAKLAGRKLIESRDIDTRSGV
FASKVIPGSPVKIELIDAGTKELVEVLEVDAVLVATGRVPTSADLNLAAVGVESERGFIPVDDGLRVLAGGNPVPHLWAV
GDVTGKLMLAHTAAAQGVVAVENICGGNRTVDYRSIPAATFTHPEISSVGLSEADAKAIAAEQGFELGSVRSYFKANSKA
LAELESDGLMKLLFRKDTGEVLGAHIFGLHAADLIQEVANAVARRQSVRDLVYEVHTHPTLSEVVESAYKQAAHALAA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=471, Percent_Identity=34.8195329087049, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI50301238, Length=479, Percent_Identity=30.062630480167, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI33519430, Length=502, Percent_Identity=28.2868525896414, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI33519428, Length=502, Percent_Identity=28.2868525896414, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI33519426, Length=502, Percent_Identity=28.2868525896414, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI148277065, Length=502, Percent_Identity=28.2868525896414, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI148277071, Length=502, Percent_Identity=28.2868525896414, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI22035672, Length=482, Percent_Identity=29.4605809128631, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI291045266, Length=499, Percent_Identity=28.8577154308617, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI291045268, Length=495, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=33.3333333333333, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI87082354, Length=484, Percent_Identity=29.1322314049587, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789915, Length=458, Percent_Identity=28.3842794759825, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI87081717, Length=475, Percent_Identity=27.1578947368421, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32565766, Length=477, Percent_Identity=33.5429769392034, Blast_Score=227, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17557007, Length=493, Percent_Identity=28.1947261663286, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=475, Percent_Identity=29.2631578947368, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=475, Percent_Identity=29.2631578947368, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71982272, Length=502, Percent_Identity=28.0876494023904, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=496, Percent_Identity=32.258064516129, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=29.8538622129436, Blast_Score=173, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=479, Percent_Identity=28.1837160751566, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21358499, Length=481, Percent_Identity=34.3035343035343, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24640549, Length=491, Percent_Identity=29.1242362525458, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640553, Length=493, Percent_Identity=29.0060851926978, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640551, Length=491, Percent_Identity=29.1242362525458, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17737741, Length=501, Percent_Identity=27.3453093812375, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50429; Mature: 50298

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAMDYDLIVIGAGYGGFDAAKHAAEKGLKTAIIESREMGGTCVNRGCVPSKALLAASG
CCCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHCCCCCCCHHHHHCCC
RVRELSDGEHLSSFGITPGTVQFDRQAIADHATQLVENIRANLTKSLERAGVTIVRGTAQ
CCEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHH
LAGPQQVAVRQSNGVERVLSATDVLIATGSDPFVPRGIETDGLTVFTSDDAVRLESLPQW
HCCCHHEEEECCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHH
LAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDIAKLAGRKLIESRDIDTRSG
HHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
VFASKVIPGSPVKIELIDAGTKELVEVLEVDAVLVATGRVPTSADLNLAAVGVESERGFI
CEECCCCCCCCCEEEEECCCHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEEECCCCCEE
PVDDGLRVLAGGNPVPHLWAVGDVTGKLMLAHTAAAQGVVAVENICGGNRTVDYRSIPAA
ECCCCCEEEECCCCCCCEEEECCCCCEEEEEEHHHHCCCEEEHHHCCCCCEEEECCCCCC
TFTHPEISSVGLSEADAKAIAAEQGFELGSVRSYFKANSKALAELESDGLMKLLFRKDTG
CCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCH
EVLGAHIFGLHAADLIQEVANAVARRQSVRDLVYEVHTHPTLSEVVESAYKQAAHALAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAAMDYDLIVIGAGYGGFDAAKHAAEKGLKTAIIESREMGGTCVNRGCVPSKALLAASG
CCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHCCCCCCCHHHHHCCC
RVRELSDGEHLSSFGITPGTVQFDRQAIADHATQLVENIRANLTKSLERAGVTIVRGTAQ
CCEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHH
LAGPQQVAVRQSNGVERVLSATDVLIATGSDPFVPRGIETDGLTVFTSDDAVRLESLPQW
HCCCHHEEEECCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHH
LAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDIAKLAGRKLIESRDIDTRSG
HHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
VFASKVIPGSPVKIELIDAGTKELVEVLEVDAVLVATGRVPTSADLNLAAVGVESERGFI
CEECCCCCCCCCEEEEECCCHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEEECCCCCEE
PVDDGLRVLAGGNPVPHLWAVGDVTGKLMLAHTAAAQGVVAVENICGGNRTVDYRSIPAA
ECCCCCEEEECCCCCCCEEEECCCCCEEEEEEHHHHCCCEEEHHHCCCCCEEEECCCCCC
TFTHPEISSVGLSEADAKAIAAEQGFELGSVRSYFKANSKALAELESDGLMKLLFRKDTG
CCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCH
EVLGAHIFGLHAADLIQEVANAVARRQSVRDLVYEVHTHPTLSEVVESAYKQAAHALAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231; 9387233 [H]